File: sixFrameTranslation.pl

package info (click to toggle)
trinityrnaseq 2.11.0%2Bdfsg-6
  • links: PTS, VCS
  • area: main
  • in suites: bullseye
  • size: 417,528 kB
  • sloc: perl: 48,420; cpp: 17,749; java: 12,695; python: 3,124; sh: 1,030; ansic: 983; makefile: 688; xml: 62
file content (37 lines) | stat: -rwxr-xr-x 766 bytes parent folder | download | duplicates (2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
#!/usr/bin/env perl

use strict;
use warnings;

use lib ("/usr/lib/trinityrnaseq/PerlLib");
use Fasta_reader;
use Nuc_translator;


my $usage = "\nn\\tusage: $0 nucFasta  \n\n";

my $fasta_file = $ARGV[0] or die $usage;

main: {
	my $fasta_reader = new Fasta_reader($fasta_file);
	while (my $seq_obj = $fasta_reader->next()) {
		
		my $accession = $seq_obj->get_accession();
		my $sequence = $seq_obj->get_sequence();
		my $header = $seq_obj->get_header();
		
		for my $frame (1..6) {
            my $translation = &translate_sequence($sequence, $frame);
            
            $translation =~ s/(\S{60})/$1\n/g;
            chomp $translation;
            
            print ">$accession.F${frame}_trans $header\n$translation\n";
        }
    }
	
	exit(0);
}