1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
|
#!/usr/bin/env perl
use strict;
use warnings;
use lib ("/usr/lib/trinityrnaseq/PerlLib");
use Fasta_reader;
use Nuc_translator;
my $usage = "\n\nusage: $0 transcripts.fa\n\n";
my $transcripts_file = $ARGV[0] or die $usage;
main: {
my $fasta_reader = new Fasta_reader($transcripts_file);
while (my $seq_obj = $fasta_reader->next()) {
my $acc = $seq_obj->get_accession();
my $seq = $seq_obj->get_sequence();
print ">$acc\n$seq\n";
my $revc_seq = &reverse_complement($seq);
print ">$acc-ANTI\n$revc_seq\n";
}
exit(0);
}
|
