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#!/usr/bin/env perl
use strict;
use warnings;
my $usage = "usage: $0 expr.RSEM\n\n";
my $expr_file = $ARGV[0] or die $usage;
open (my $fh, $expr_file) or die $!;
my $header = <$fh>;
my @fpkms;
while (<$fh>) {
chomp;
my @x = split(/\t/);
my $fpkm = $x[6];
push (@fpkms, $fpkm);
}
@fpkms = reverse sort {$a<=>$b} @fpkms;
my $min_fpkm_thresh = int($fpkms[0] + 0.5);
my $num_features = 1;
print "neg_min_fpkm\tnum_features\n";
shift @fpkms;
while (@fpkms) {
my $fpkm = shift @fpkms;
$fpkm = int($fpkm+0.5);
if ($fpkm < $min_fpkm_thresh) {
print "" . (-1*$min_fpkm_thresh) . "\t$num_features\n";
$min_fpkm_thresh = $fpkm;
}
$num_features++;
}
print "" . (-1*$min_fpkm_thresh) . "\t$num_features\n";
exit(0);
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