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<?xml version="1.0" ?>
<multi-test>
<load-document index="doc" url="primer3/primer3_xml/internal/boundary_test_00.fa" io="local_file" format="fasta"/>
<find-object-by-name index="seq" doc="doc" name="boundary_test_00" type="OT_SEQUENCE"/>
<plugin_primer_3 sequence="seq">
<plugin_primer_3_in
PRIMER_MAX_POLY_X="0"
PRIMER_INTERNAL_MAX_POLY_X="0"
PRIMER_EXPLAIN_FLAG="1"
PRIMER_PRODUCT_SIZE_RANGE="50-100"
PRIMER_MIN_TM="0.0"
PRIMER_MAX_TM="100.0"
PRIMER_MIN_SIZE="25"
PRIMER_MAX_SIZE="25"
PRIMER_INTERNAL_MIN_SIZE="25"
PRIMER_INTERNAL_MAX_SIZE="25"
PRIMER_INTERNAL_OPT_SIZE="25"
PRIMER_OPT_SIZE="25"
PRIMER_MAX_GC="100"
PRIMER_MIN_GC="0"
PRIMER_MAX_SELF_END="100"
PRIMER_MAX_SELF_ANY="100"
PRIMER_PAIR_MAX_COMPL_ANY="100"
PRIMER_PAIR_MAX_COMPL_END="100"
PRIMER_INTERNAL_MAX_SELF_ANY="100"
PRIMER_INTERNAL_MAX_SELF_END="100"
PRIMER_INTERNAL_MAX_TM="100"
PRIMER_INTERNAL_MIN_TM="30"
PRIMER_INTERNAL_MAX_GC="100"
PRIMER_INTERNAL_MIN_GC="0"
PRIMER_PICK_INTERNAL_OLIGO="1"/>
<plugin_primer_3_out
PRIMER_LEFT_NUM_RETURNED="1"
PRIMER_RIGHT_NUM_RETURNED="1"
PRIMER_INTERNAL_NUM_RETURNED="1"
PRIMER_PAIR_NUM_RETURNED="1"
PRIMER_PAIR_0_PENALTY="3.381274"
PRIMER_LEFT_0_PENALTY="2.731673"
PRIMER_RIGHT_0_PENALTY="0.649600"
PRIMER_INTERNAL_0_PENALTY="11.495378"
PRIMER_LEFT_0_SEQUENCE="CACGTCATCTCATCAAATTTGATTG"
PRIMER_RIGHT_0_SEQUENCE="GAATTGTTTGGAAATGACAGTGAGT"
PRIMER_INTERNAL_0_SEQUENCE="ACGTACGTACGTTGCAGTCGACGCA"
PRIMER_LEFT_0="2,25"
PRIMER_RIGHT_0="81,25"
PRIMER_INTERNAL_0="30,25"
PRIMER_LEFT_0_TM="62.732"
PRIMER_RIGHT_0_TM="60.650"
PRIMER_INTERNAL_0_TM="71.495"
PRIMER_LEFT_0_GC_PERCENT="36.000"
PRIMER_RIGHT_0_GC_PERCENT="36.000"
PRIMER_INTERNAL_0_GC_PERCENT="56.000"
PRIMER_LEFT_0_SELF_ANY="12.00"
PRIMER_RIGHT_0_SELF_ANY="4.00"
PRIMER_INTERNAL_0_SELF_ANY="12.00"
PRIMER_LEFT_0_SELF_END="10.00"
PRIMER_RIGHT_0_SELF_END="3.00"
PRIMER_INTERNAL_0_SELF_END="8.00"
PRIMER_LEFT_0_END_STABILITY="6.9000"
PRIMER_RIGHT_0_END_STABILITY="6.4000"
PRIMER_PAIR_0_COMPL_ANY="7.00"
PRIMER_PAIR_0_COMPL_END="1.00"
PRIMER_PAIR_0_PRODUCT_SIZE="80"
PRIMER_PAIR_0_PRODUCT_TM="68.8"/>
</plugin_primer_3>
</multi-test>
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