1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
|
<?xml version="1.0" ?>
<multi-test>
<load-document index="doc" url="primer3/primer3_xml/start_codon/test0.fa" io="local_file" format="fasta"/>
<find-object-by-name index="seq" doc="doc" name="test0" type="OT_SEQUENCE"/>
<plugin_primer_3 sequence="seq">
<plugin_primer_3_in
PRIMER_PRODUCT_SIZE_RANGE="75-20000"
PRIMER_MAX_END_STABILITY="10"
PRIMER_EXPLAIN_FLAG="1"
PRIMER_MIN_SIZE="19"
PRIMER_MAX_TM="75"
PRIMER_NUM_RETURN="1"
SEQUENCE_START_CODON_POSITION="-3"/>
<plugin_primer_3_out
PRIMER_STOP_CODON_POSITION="75"
PRIMER_LEFT_NUM_RETURNED="1"
PRIMER_RIGHT_NUM_RETURNED="1"
PRIMER_INTERNAL_NUM_RETURNED="0"
PRIMER_PAIR_NUM_RETURNED="1"
PRIMER_PAIR_0_PENALTY="69.797698"
PRIMER_LEFT_0_PENALTY="68.113745"
PRIMER_RIGHT_0_PENALTY="1.683953"
PRIMER_LEFT_0_SEQUENCE="ATTGAGAATAGTCTTAGATGTGTCGAA"
PRIMER_RIGHT_0_SEQUENCE="ACTGAGCAAGGAAGCTCCG"
PRIMER_LEFT_0="0,27"
PRIMER_RIGHT_0="75,19"
PRIMER_LEFT_0_TM="58.886"
PRIMER_RIGHT_0_TM="60.684"
PRIMER_LEFT_0_GC_PERCENT="33.333"
PRIMER_RIGHT_0_GC_PERCENT="57.895"
PRIMER_LEFT_0_SELF_ANY="6.00"
PRIMER_RIGHT_0_SELF_ANY="4.00"
PRIMER_LEFT_0_SELF_END="2.00"
PRIMER_RIGHT_0_SELF_END="2.00"
PRIMER_LEFT_0_POSITION_PENALTY="60.000000"
PRIMER_RIGHT_0_POSITION_PENALTY="0.000000"
PRIMER_LEFT_0_END_STABILITY="8.7000"
PRIMER_RIGHT_0_END_STABILITY="9.9000"
PRIMER_PAIR_0_COMPL_ANY="3.00"
PRIMER_PAIR_0_COMPL_END="2.00"
PRIMER_PAIR_0_PRODUCT_SIZE="76"
PRIMER_PAIR_0_PRODUCT_TM="70.9"/>
</plugin_primer_3>
</multi-test>
|
