File: data.rst

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.. _data:

Data
======

You can find the links to all preprocessed data used in our manuscript at Zenodo `for the SGNEx data <https://doi.org/10.5281/zenodo.4604945>`_ and `for the others samples <https://doi.org/10.5281/zenodo.4587661>`_.
All the raw fast5 and fastq files are also available at `ENA <https://www.ebi.ac.uk/ena/browser/view/PRJEB40872>`_ and `SGNEx <https://github.com/GoekeLab/sg-nex-data>`_. Please refer to our Supplementary Table S7 in our manuscript for full details of data download.


Note that all HEK293T-KO samples can be used as unmodified (m6A) controls for any other data set generated with the same RNA kit (SQK-RNA001).
If the cells are genetically different, we recommend to perform variant calling before finalising the list of differentially modified sites in order to remove false positives.