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package jgi;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.BitSet;
import java.util.HashMap;
import java.util.HashSet;
import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextFile;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ArrayListSet;
import stream.ConcurrentGenericReadInputStream;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.MultiCros;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* @author Brian Bushnell
* @date Oct 9, 2014
*
*/
public class DemuxByName {
public static void main(String[] args){
final int oldCap=Shared.numBuffers(), oldZipThreads=ReadWrite.MAX_ZIP_THREADS(), oldZl=ReadWrite.ZIPLEVEL;
final boolean oldPigz=ReadWrite.USE_PIGZ, oldUnpigz=ReadWrite.USE_UNPIGZ;
Timer t=new Timer();
DemuxByName x=new DemuxByName(args);
x.process(t);
Shared.setBuffers(oldCap);
ReadWrite.ZIPLEVEL=oldZl;
ReadWrite.USE_PIGZ=oldPigz;
ReadWrite.USE_UNPIGZ=oldUnpigz;
ReadWrite.setZipThreads(oldZipThreads);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public DemuxByName(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
boolean setInterleaved=false; //Whether it was explicitly set.
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
ReadWrite.ZIPLEVEL=1;
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){
//do nothing
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
ByteFile2.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
ConcurrentGenericReadInputStream.verbose=verbose;
stream.FastqReadInputStream.verbose=verbose;
ReadWrite.verbose=verbose;
}else if(a.equals("names") || a.equals("name") || a.equals("affixes")){
if(b!=null){
String[] x=b.split(",");
for(String s : x){
names.add(s);
}
}
}else if(a.equalsIgnoreCase("length") || a.equalsIgnoreCase("len") || a.equalsIgnoreCase("affixlength") || a.equalsIgnoreCase("affixlen")){
fixedAffixLength=Integer.parseInt(b);
}else if(a.equals("prefixmode") || a.equals("prefix") || a.equals("pm")){
prefixMode=Parse.parseBoolean(b);
}else if(a.equals("suffixmode") || a.equals("suffix") || a.equals("sm")){
prefixMode=!Parse.parseBoolean(b);
}else if(a.equals("column")){
column=Integer.parseInt(b);
assert(column>0 || column==-1) : "Column is 1-based; must be 1+ or else -1 to disable.";
column--;
}else if(a.equals("substringmode") || a.equals("substring")){
substringMode=Parse.parseBoolean(b);
}else if(a.equals("outu") || a.equals("outu1")){
outu1=b;
}else if(a.equals("outu2")){
outu2=b;
}else if(a.equals("perheader") || a.equals("persequence") || a.equals("pername")){
perheader=Parse.parseBoolean(b);
}else if(a.equals("delimiter")){
delimiter=Parse.parseSymbol(b);
}else if(parser.in1==null && i==0 && Tools.looksLikeInputStream(arg)){
parser.in1=arg;
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
if(perheader){
ReadWrite.USE_PIGZ=false;
}else{
String[] x=names.toArray(new String[names.size()]);
names.clear();
for(String s : x){
File f=new File(s);
if(f.exists() && f.isFile()){
TextFile tf=new TextFile(s);
String[] lines=tf.toStringLines();
for(String s2 : lines){
names.add(s2);
}
}else{
names.add(s);
}
}
// for(String s : names){
// assert(affixLen<0 || affixLen==s.length()) : "All names must have the same length.";
// affixLen=s.length();
// }
// assert(affixLen>0) : "Must include at least one non-zero-length affix (name).";
{
BitSet bs=new BitSet();
if(fixedAffixLength>0){
bs.set(fixedAffixLength);
}
for(String s : names){
bs.set(s.length());
}
affixLengths=new int[bs.cardinality()];
for(int i=0, bit=-1; i<affixLengths.length; i++){
bit=bs.nextSetBit(bit+1);
affixLengths[i]=bit;
}
Arrays.sort(affixLengths);
Tools.reverseInPlace(affixLengths);
}
assert((affixLengths.length>0 && affixLengths[0]>0) || delimiter!=null || perheader) :
"Must include at least one non-zero-length affix (name), or a delimiter.";
ReadWrite.setZipThreads(Tools.max(1, Tools.min(ReadWrite.MAX_ZIP_THREADS(), (Shared.threads()*2-1)/Tools.max(1, names.size()))));
if(names.size()>8){ReadWrite.USE_PIGZ=false;}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
setInterleaved=parser.setInterleaved;
in1=parser.in1;
in2=parser.in2;
qfin1=parser.qfin1;
qfin2=parser.qfin2;
out1=parser.out1;
out2=parser.out2;
qfout1=parser.qfout1;
qfout2=parser.qfout2;
extin=parser.extin;
extout=parser.extout;
}
assert(out1==null || out1.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
assert(out2==null || out2.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
assert(qfout1==null || qfout1.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
assert(qfout2==null || qfout2.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
if(outu1!=null && outu2==null && outu1.indexOf('#')>-1){
outu2=outu1.replace("#", "2");
outu1=outu1.replace("#", "1");
}
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){outstream.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
if(!setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}
}
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(out2!=null && out2.equalsIgnoreCase("null")){out2=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2, outu1, outu2)){
outstream.println((out1==null)+", "+(out2==null)+", "+out1+", "+out2+", "+outu1+", "+outu2);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+", "+outu1+", "+outu2+"\n");
}
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
if(ffin1!=null && out1!=null && ffin1.samOrBam()){
useSharedHeader=FileFormat.isSamOrBamFile(out1);
}
if(perheader){
fixedAffixLength=-1;
substringMode=false;
affixLengths=null;
delimiter=null;
}
}
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, useSharedHeader, ffin1, ffin2, qfin1, qfin2);
if(verbose){outstream.println("Started cris");}
cris.start(); //4567
}
boolean paired=cris.paired();
if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
final MultiCros mcros;
if(out1!=null){
final int buff=4;
mcros=(fixedAffixLength>0 || delimiter!=null || perheader ?
new MultiCros(out1, out2, false, overwrite, append, true, useSharedHeader, FileFormat.FASTQ, buff) : null);
if(paired && out2==null && (in1==null || !in1.contains(".sam"))){
outstream.println("Writing interleaved.");
}
assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
assert(out2==null || (!out2.equalsIgnoreCase(in1) && !out2.equalsIgnoreCase(in2))) : "out1 and out2 have same name.";
for(String s : names){
String qf1=null, qf2=null;
if(qfout1!=null){qf1=qfout1.replace("%", s);}
if(qfout2!=null){qf2=qfout2.replace("%", s);}
FileFormat ffout1=FileFormat.testOutput(out1.replace("%", s), FileFormat.FASTQ, extout, true, overwrite, append, false);
FileFormat ffout2=(out2==null ? null : FileFormat.testOutput(out2.replace("%", s), FileFormat.FASTQ, extout, true, overwrite, append, false));
ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, qf1, qf2, buff, null, true);
ros.start();
nameToStream.put(s, ros);
}
}else{
mcros=null;
}
final ConcurrentReadOutputStream rosu;
if(outu1!=null){
final int buff=4;
FileFormat ffout1=FileFormat.testOutput(outu1, FileFormat.FASTQ, extout, true, overwrite, append, false);
FileFormat ffout2=(outu2==null ? null : FileFormat.testOutput(outu2, FileFormat.FASTQ, extout, true, overwrite, append, false));
rosu=ConcurrentReadOutputStream.getStream(ffout1, ffout2, null, null, buff, null, true);
rosu.start();
}else{
rosu=null;
}
long readsProcessed=0;
long basesProcessed=0;
long readsOut=0;
long basesOut=0;
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
// outstream.println("Fetched "+reads);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
for(String s : names){
nameToArray.put(s, new ArrayList<Read>());
}
final ArrayListSet als=(fixedAffixLength<1 && delimiter==null && !perheader ? null : new ArrayListSet(false));
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
ArrayList<Read> unmatched=new ArrayList<Read>();
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
final Read r2=r1.mate;
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
final String id=r1.id;
final int idlen=id.length();
ArrayList<Read> al2=null;
if(names.size()>0){
if(substringMode){
for(String s : names){
if(id.contains(s)){
al2=nameToArray.get(s);
break;
}
}
}else{
for(int affixLen : affixLengths){
final String sub=idlen>=affixLen ? prefixMode ? id.substring(0, affixLen) : id.substring(idlen-affixLen) : id;
al2=nameToArray.get(sub);
if(al2!=null){break;}
}
}
}
if(al2!=null || als!=null){
readsOut++;
basesOut+=initialLength1;
if(r2!=null){
readsOut++;
basesOut+=initialLength2;
}
if(al2!=null){
al2.add(r1);
{
readsOut++;
basesOut+=initialLength1;
}
if(r2!=null){
readsOut++;
basesOut+=initialLength2;
}
}else if(als!=null){
String sub=r1.id;
if(fixedAffixLength>0){
sub=(sub.length()<=fixedAffixLength ? sub : prefixMode ? id.substring(0, fixedAffixLength) : id.substring(idlen-fixedAffixLength));
}else if(delimiter!=null) {
String[] split=sub.split(delimiter);
assert(split.length>1) : "Delimiter '"+delimiter+"' was not found in name '"+sub+"'";
if(column>-1){
int col=Tools.min(column, split.length-1);
sub=split[col];
if(col!=column && !warned){
System.err.println("*** WARNING! ***\n"
+ "Only "+(col+1)+" columns for record "+r1.id+"\n"
+ "Further warnings will be suppressed.\n");
warned=true;
assert(errorState=true); //Change error state to true if assertions are enabled.
}
}else{
sub=split[prefixMode ? 0 : split.length-1];
}
}else{
assert(perheader);
//Use the full ID
}
als.add(r1, sub);
}
}else{
unmatched.add(r1);
}
{
readsProcessed++;
basesProcessed+=initialLength1;
}
if(r2!=null){
readsProcessed++;
basesProcessed+=initialLength2;
}
}
for(String s : names){
ArrayList<Read> listOut=nameToArray.put(s, new ArrayList<Read>());
ConcurrentReadOutputStream ros=nameToStream.get(s);
if(ros!=null){ros.add(listOut, ln.id);}
}
if(mcros!=null && als!=null){
if(als.size()+names.size()>8){ReadWrite.USE_PIGZ=false;}
mcros.add(als, ln.id);
}
if(rosu!=null){rosu.add(unmatched, ln.id);}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
errorState|=ReadStats.writeAll();
errorState|=ReadWrite.closeStream(cris);
for(String s : names){
ConcurrentReadOutputStream ros=nameToStream.get(s);
errorState|=ReadWrite.closeStream(ros);
}
if(mcros!=null){
errorState|=ReadWrite.closeStreams(mcros);
}
t.stop();
double rpnano=readsProcessed/(double)(t.elapsed);
double bpnano=basesProcessed/(double)(t.elapsed);
outstream.println("Time: "+t);
outstream.println("Reads Processed: "+readsProcessed+" \t"+Tools.format("%.2fk reads/sec", rpnano*1000000));
outstream.println("Bases Processed: "+basesProcessed+" \t"+Tools.format("%.2fm bases/sec", bpnano*1000));
outstream.println("Reads Out: "+readsOut);
outstream.println("Bases Out: "+basesOut);
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
private String in1=null;
private String in2=null;
private String qfin1=null;
private String qfin2=null;
private String out1=null;
private String out2=null;
private String outu1=null;
private String outu2=null;
private String qfout1=null;
private String qfout2=null;
private String extin=null;
private String extout=null;
/*--------------------------------------------------------------*/
private long maxReads=-1;
// private boolean exclude=true;
private String delimiter=null;
private boolean prefixMode=true;
private boolean substringMode=false;
private boolean perheader=false;
private int column=-1;
private boolean warned=false;
// private int affixLen=-1;
private int fixedAffixLength=-1;
private int[] affixLengths;
private HashSet<String> names=new HashSet<String>();
private HashMap<String, ArrayList<Read>> nameToArray=new HashMap<String, ArrayList<Read>>();
private HashMap<String, ConcurrentReadOutputStream> nameToStream=new HashMap<String, ConcurrentReadOutputStream>();
/*--------------------------------------------------------------*/
private final FileFormat ffin1;
private final FileFormat ffin2;
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
private boolean useSharedHeader=false;
}
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