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package jgi;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import dna.AminoAcid;
import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.KillSwitch;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* @author Brian Bushnell
* @date Sep 11, 2012
*
*/
public class FakeReads {
public static void main(String[] args){
Timer t=new Timer();
FakeReads x=new FakeReads(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public FakeReads(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(a.equals("passes")){
assert(false) : "'passes' is disabled.";
// passes=Integer.parseInt(b);
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
ByteFile2.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
ConcurrentGenericReadInputStream.verbose=verbose;
stream.FastqReadInputStream.verbose=verbose;
ReadWrite.verbose=verbose;
}else if(a.equals("addspacer") || a.equals("addspace") || a.equals("usespacer")){
addSpacer=Parse.parseBoolean(b);
}else if(a.equals("reads") || a.equals("maxreads")){
maxReads=Parse.parseKMG(b);
}else if(a.equals("t") || a.equals("threads")){
Shared.setThreads(b);
}else if(a.equals("in") || a.equals("input") || a.equals("in1") || a.equals("input1")){
in1=b;
}else if(a.equals("out") || a.equals("output") || a.equals("out1") || a.equals("output1")){
out1=b;
}else if(a.equals("out2") || a.equals("output2")){
out2=b;
}else if(a.equals("identifier") || a.equals("id")){
identifier=b;
}else if(a.equals("qfin") || a.equals("qfin1")){
qfin1=b;
}else if(a.equals("qfout") || a.equals("qfout1")){
qfout1=b;
}else if(a.equals("qfout2")){
qfout2=b;
}else if(a.equals("extin")){
extin=b;
}else if(a.equals("extout")){
extout=b;
}else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
}else if(a.startsWith("minscaf") || a.startsWith("mincontig")){
stream.FastaReadInputStream.MIN_READ_LEN=Integer.parseInt(b);
}else if(a.equals("ml") || a.equals("minlen") || a.equals("minlength")){
minReadLength=Integer.parseInt(b);
}else if(a.equals("length") || a.equals("maxlen") || a.equals("length")){
desiredLength=Integer.parseInt(b);
}else if(a.equals("split")){
SPLITMODE=Parse.parseBoolean(b);
}else if(a.equals("overlap")){
SPLITMODE=true;
overlap=Integer.parseInt(b);
}else if(in1==null && i==0 && Tools.looksLikeInputStream(arg)){
in1=arg;
if(arg.indexOf('#')>-1 && !new File(arg).exists() && b!=null){//b!=null is implied
in1=b.replace("#", "1");
}
}else{
System.err.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
}
if(identifier==null){identifier="";}
else{identifier=identifier+"_";}
if(!addSpacer){spacer="";}
// if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
// in2=in1.replace("#", "2");
// in1=in1.replace("#", "1");
// }
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
// if(in2!=null){
// if(FASTQ.FORCE_INTERLEAVED){System.err.println("Reset INTERLEAVED to false because paired input files were specified.");}
// FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
// }
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
// if(ReadWrite.isCompressed(in1)){ByteFile.FORCE_MODE_BF2=true;}
ByteFile.FORCE_MODE_BF2=true;
}
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(out2!=null && out2.equalsIgnoreCase("null")){out2=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
}
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, null, qfin1, null);
if(verbose){System.err.println("Started cris");}
cris.start(); //4567
}
boolean paired=cris.paired();
if(verbose){System.err.println("Input is "+(paired ? "paired" : "unpaired"));}
ConcurrentReadOutputStream ros=null;
if(out1!=null){
final int buff=4;
if(cris.paired() && out2==null && (in1==null || !in1.contains(".sam"))){
outstream.println("Writing interleaved.");
}
assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
assert(out2==null || (!out2.equalsIgnoreCase(in1) && !out2.equalsIgnoreCase(out1))) : "out1 and out2 have same name.";
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, qfout1, qfout2, buff, null, false);
ros.start();
}
long readsProcessed=0;
long basesProcessed=0;
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
ArrayList<Read> fake=new ArrayList<Read>(reads.size());
for(int idx=0; idx<reads.size(); idx++){
Read r=reads.get(idx);
{
readsProcessed++;
basesProcessed+=r.length();
}
assert(r.mate==null);
boolean remove=r.length()<minReadLength || (minReadLength+overlap)<2;
if(remove){
//Do nothing
}else{
int len=Tools.min(r.length(), desiredLength);
if(SPLITMODE){len=Tools.min(r.length(), (r.length()+overlap+1)/2);}
byte[] bases1=KillSwitch.copyOfRange(r.bases, 0, len);
byte[] bases2=KillSwitch.copyOfRange(r.bases, r.length()-len, r.length());
AminoAcid.reverseComplementBasesInPlace(bases2);
byte[] qual1=null;
byte[] qual2=null;
if(r.quality!=null){
qual1=KillSwitch.copyOfRange(r.quality, 0, len);
qual2=KillSwitch.copyOfRange(r.quality, r.quality.length-len, r.quality.length);
Tools.reverseInPlace(qual2);
}
// public Read(byte[] s_, int chrom_, int start_, int stop_, String id_, byte[] quality_, long numericID_, int flags_){
Read a=new Read(bases1, qual1, identifier+r.numericID+spacer+"/1", r.numericID, 0);
Read b=new Read(bases2, qual2, identifier+r.numericID+spacer+"/2", r.numericID, Read.PAIRNUMMASK);
a.mate=b;
b.mate=a;
fake.add(a);
}
}
if(ros!=null){ros.add(fake, ln.id);}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
errorState|=ReadWrite.closeStreams(cris, ros);
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
if(errorState){
throw new RuntimeException("FakeReads terminated in an error state; the output may be corrupt.");
}
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
public boolean errorState=false;
public String identifier=null;
private String in1=null;
private boolean addSpacer=true;
private String spacer=" ";
private String qfin1=null;
private String out1=null;
private String out2=null;
private String qfout1=null;
private String qfout2=null;
private String extin=null;
private String extout=null;
private boolean overwrite=true;
private boolean append=false;
private long maxReads=-1;
private int minReadLength=1;
private int desiredLength=250;
private int overlap=50;
private boolean SPLITMODE=false;
private final FileFormat ffin1;
private final FileFormat ffout1;
private final FileFormat ffout2;
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
}
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