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package jgi;
import java.util.ArrayList;
import shared.Parse;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.ListNum;
import template.BBTool_ST;
/**
* Fuses sequences together, with N-padding in between.
* @author Brian Bushnell
* @date Jan 20, 2015
*
*/
public final class FuseSequence extends BBTool_ST {
public static void main(String[] args){
Timer t=new Timer();
FuseSequence fs=new FuseSequence(args);
fs.process(t);
}
public FuseSequence(String[] args){
super(args);
reparse(args);
if(PAD_SYMBOL==0){PAD_SYMBOL='N';}
if(amino){
Shared.AMINO_IN=true;
if(PAD_SYMBOL=='N'){PAD_SYMBOL='X';}
}
}
@Override
protected void setDefaults(){
npad=300;
defaultQuality=30;
fusePairs=false;
PAD_SYMBOL=0;
amino=false;
}
@Override
public boolean parseArgument(String arg, String a, String b) {
if(a.equals("pad") || a.equals("npad") || a.equals("ns")){
npad=Integer.parseInt(b);
return true;
}else if(a.equals("q") || a.equals("quality")){
defaultQuality=Byte.parseByte(b);
return true;
}else if(a.equals("fp") || a.equals("fusepairs")){
fusePairs=Parse.parseBoolean(b);
return true;
}else if(a.equals("maxlen")){
maxlen=(int)Tools.min(Parse.parseKMG(b), Shared.MAX_ARRAY_LEN);
return true;
}else if(a.equals("rename") || a.equals("name") || a.equals("prefix")){
name=(b==null ? "" : b);
return true;
}else if(a.equals("addnumber")){
addNumber=Parse.parseBoolean(b);
return true;
}else if(a.equals("padsymbol")){
PAD_SYMBOL=b.charAt(0);
return true;
}else if(a.equals("amino")){
amino=Parse.parseBoolean(b);
return true;
}
return false;
}
@Override
protected void processInner(final ConcurrentReadInputStream cris, final ConcurrentReadOutputStream ros){
readsProcessed=0;
basesProcessed=0;
long outNum=0;
long lastListID=0;
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
lastListID=ln.id;
ArrayList<Read> readsOut=new ArrayList<Read>(reads.size());
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
final Read r2=r1.mate;
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
{
readsProcessed++;
basesProcessed+=initialLength1;
}
if(r2!=null){
readsProcessed++;
basesProcessed+=initialLength2;
}
if(!fusePairs && maxlen>0 && bases.length>0 && bases.length+initialLength1+initialLength2+npad>maxlen){
Read r=bufferToRead(outNum);
outNum++;
readsOut.add(r);
}
processReadPair(r1, r2);
if(fusePairs){
readsOut.add(r1);
}else if(maxlen>=0 && bases.length>=maxlen){
Read r=bufferToRead(outNum);
outNum++;
readsOut.add(r);
}
}
if(ros!=null && (fusePairs || maxlen>0)){ros.add(readsOut, ln.id);}
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
if(ros!=null && bases.length()>0){
ArrayList<Read> readsOut=new ArrayList<Read>(1);
Read r=bufferToRead(outNum);
outNum++;
readsOut.add(r);
ros.add(readsOut, lastListID);
}
}
Read bufferToRead(long id){
Read r=new Read(bases.toBytes(), (quals==null ? null : quals.toBytes()), 0);
bases.clear();
if(quals!=null){quals.clear();}
r.id=(name==null || name.length()==0 ? ""+id : (addNumber ? name+" "+id : name));
addNumber=true;
return r;
}
/* (non-Javadoc)
* @see jgi.BBTool_ST#processReadPair(stream.Read, stream.Read)
*/
@Override
protected boolean processReadPair(Read r1, Read r2) {
if(fusePairs){
fusePair(r1, r2);
return true;
}
if(r1!=null && r1.length()>0){processRead(r1);}
if(r2!=null && r2.length()>0){processRead(r2);}
return false;
}
private void fusePair(Read r1, Read r2) {
if(r2==null){return;}
r2.reverseComplement();
final int len=r1.length()+r2.length()+npad;
byte[] bases=new byte[len];
byte[] quals=(r1.quality==null || r2.quality==null ? null : new byte[len]);
for(int i=0, max=r1.length(); i<max; i++){
bases[i]=r1.bases[i];
if(quals!=null){quals[i]=r1.quality[i];}
}
for(int i=0, j=r1.length(); i<npad; i++, j++){
bases[j]=(byte)PAD_SYMBOL;
}
for(int i=0, j=r1.length()+npad, max=r2.length(); i<max; i++, j++){
bases[j]=r2.bases[i];
if(quals!=null){quals[j]=r2.quality[i];}
}
r1.mate=r2.mate=null;
r1.bases=bases;
r1.quality=quals;
}
private void processRead(Read r) {
if(name==null){
name=r.id;
}
if(bases.length>0){
for(int i=0; i<npad; i++){
bases.append(PAD_SYMBOL);
quals.append((byte)0);
}
}
bases.append(r.bases);
if(r.quality!=null){
quals.append(r.quality);
}else{
for(int i=0, max=r.length(); i<max; i++){
quals.append(defaultQuality);
}
}
}
@Override
protected void startupSubclass() {}
@Override
protected void shutdownSubclass() {}
@Override
protected final boolean useSharedHeader(){return false;}
@Override
protected void showStatsSubclass(Timer t, long readsIn, long basesIn) {}
int maxlen=Shared.MAX_ARRAY_LEN;
int npad;
byte defaultQuality;
boolean fusePairs;
ByteBuilder bases=new ByteBuilder();
ByteBuilder quals=new ByteBuilder();
String name;
// boolean prefix=true;
boolean addNumber=false;
char PAD_SYMBOL='N';
boolean amino=false;
}
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