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package jgi;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.HashSet;
import dna.Data;
import fileIO.ByteFile1;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextStreamWriter;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* Grab reads with specified numbers from a file.
* TODO Note that much of this is ripped directly from ReformatReads, but is incorrect, because this class does not support dual output files.
* @author Brian Bushnell
* @date Jul 10, 2013
*
*/
public class GetReads {
public static void main(String[] args){
GetReads x=new GetReads(args);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public GetReads(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Timer t=new Timer();
Parser parser=new Parser();
String in1=null;
String in2=null;
String qfin1=null;
String qfin2=null;
String out1=null;
String out2=null;
String qfout1=null;
String qfout2=null;
boolean errorState=false;
long maxReads=-1;
int passes=1;
boolean testsize=false;
boolean overwrite=false, append=false;
float samplerate=1f;
long sampleseed=1;
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
HashSet<Long> table=new HashSet<Long>();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(parser.parseInterleaved(arg, a, b)){
//do nothing
}else if(a.equals("id") || a.equals("number")){
assert(b!=null) : "Bad parameter: "+arg;
String[] b2=b.split(",");
for(String c : b2){
final long x, y;
if(c.indexOf('-')>=0){
String[] c2=c.split("-");
assert(c2.length==2) : c;
x=Long.parseLong(c2[0]);
y=Long.parseLong(c2[1]);
}else{
x=y=Long.parseLong(c);
}
for(long z=x; z<=y; z++){
table.add(z);
}
}
}else if(a.equals("passes")){
passes=Integer.parseInt(b);
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
}else if(a.equals("reads") || a.startsWith("maxreads")){
maxReads=Parse.parseKMG(b);
}else if(a.equals("build") || a.equals("genome")){
Data.setGenome(Integer.parseInt(b));
}else if(a.equals("in") || a.equals("input") || a.equals("in1") || a.equals("input1")){
assert(b!=null) : "Bad parameter: "+arg;
in1=b;
if(b.indexOf('#')>-1 && !new File(b).exists()){
in1=b.replace("#", "1");
in2=b.replace("#", "2");
}
}else if(a.equals("in2") || a.equals("input2")){
in2=b;
}else if(a.equals("out") || a.equals("output") || a.equals("out1") || a.equals("output1")){
assert(b!=null) : "Bad parameter: "+arg;
out1=b;
if(b.indexOf('#')>-1){
out1=b.replace("#", "1");
out2=b.replace("#", "2");
}
}else if(a.equals("out2") || a.equals("output2")){
out2=b;
}else if(a.equals("qfin") || a.equals("qfin1")){
qfin1=b;
}else if(a.equals("qfout") || a.equals("qfout1")){
qfout1=b;
}else if(a.equals("qfin2")){
qfin2=b;
}else if(a.equals("qfout2")){
qfout2=b;
}else if(a.equals("testsize")){
testsize=Parse.parseBoolean(b);
}else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
}else if(a.equals("samplerate")){
samplerate=Float.parseFloat(b);
assert(samplerate<=1f && samplerate>=0f) : "samplerate="+samplerate+"; should be between 0 and 1";
}else if(a.equals("sampleseed")){
sampleseed=Long.parseLong(b);
}else if(a.startsWith("minscaf") || a.startsWith("mincontig")){
stream.FastaReadInputStream.MIN_READ_LEN=Integer.parseInt(b);
}else if(in1==null && i==0 && Tools.looksLikeInputStream(arg)){
in1=arg;
if(arg.indexOf('#')>-1 && !new File(arg).exists()){
in1=arg.replace("#", "1");
in2=arg.replace("#", "2");
}
}else{
System.err.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
}
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(out1==null){
if(out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
out1="stdout";
}
if(!parser.setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}
}
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(out2!=null && out2.equalsIgnoreCase("null")){out2=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
FileFormat ffin=FileFormat.testInput(in1, 0, null, true, true);
FileFormat ffout=FileFormat.testOutput(out1, 0, null, true, overwrite, append, false);
final boolean useSharedHeader=(ffin!=null && ffout!=null && ffin.samOrBam() && ffout.samOrBam());
if(ffin!=null && ffout!=null && ffin.samOrBam() && (ffout.samOrBam() || ffout.bread())){
throw new RuntimeException("\nDirect conversion of sam to sam or bread are not currently supported.\nAll other conversions are possible.");
}
ConcurrentReadInputStream cris;
{
FileFormat ff1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
FileFormat ff2=FileFormat.testInput(in2, FileFormat.FASTQ, null, true, true);
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, useSharedHeader, ff1, ff2);
}
cris.setSampleRate(samplerate, sampleseed);
outstream.println("Input is "+(cris.paired() ? "paired" : "unpaired"));
cris.start(); //4567
TextStreamWriter tsw=new TextStreamWriter(out1, overwrite, false, false);
tsw.start();
long readsProcessed=0;
long basesProcessed=0;
for(int pass=1; pass<=passes; pass++){
// outstream.println("pass="+pass);
if(pass>1){
FileFormat ff1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
FileFormat ff2=FileFormat.testInput(in2, FileFormat.FASTQ, null, true, true);
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, useSharedHeader, ff1, ff2);
cris.setSampleRate(samplerate, sampleseed);
cris.start();
}
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert(ffin==null || ffin.samOrBam() || (r.mate!=null)==cris.paired());//ffin cannot be null
}
while(reads!=null && reads.size()>0 && !table.isEmpty() && ln!=null){//ln!=null is implied
for(Read r1 : reads){
{
readsProcessed++;
basesProcessed+=r1.length();
}
Read r2=r1.mate;
if(r2!=null){
readsProcessed++;
basesProcessed+=r2.length();
}
if(table.remove(r1.numericID)){
tsw.println(r1);
if(r2!=null){tsw.println(r2);}
if(table.isEmpty()){break;}
}
}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
cris.returnList(ln);
errorState|=ReadWrite.closeStream(cris);
}
if(tsw!=null){
tsw.poisonAndWait();
}
errorState|=(cris.errorState());
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
if(testsize){
long bytesProcessed=(new File(in1).length()+(in2==null ? 0 : new File(in2).length()))*passes;
double xpnano=bytesProcessed/(double)(t.elapsed);
String xpstring=(bytesProcessed<100000 ? ""+bytesProcessed : bytesProcessed<100000000 ? (bytesProcessed/1000)+"k" : (bytesProcessed/1000000)+"m");
while(xpstring.length()<8){xpstring=" "+xpstring;}
outstream.println("Bytes Processed: "+xpstring+" \t"+Tools.format("%.2fm bytes/sec", xpnano*1000));
}
if(errorState){
throw new RuntimeException("GetReads terminated in an error state; the output may be corrupt.");
}
}
private PrintStream outstream=System.err;
public static boolean verbose=false;
}
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