File: GetReads.java

package info (click to toggle)
bbmap 39.20%2Bdfsg-3
  • links: PTS, VCS
  • area: main
  • in suites: forky, sid
  • size: 26,024 kB
  • sloc: java: 312,743; sh: 18,099; python: 5,247; ansic: 2,074; perl: 96; makefile: 39; xml: 38
file content (310 lines) | stat: -rwxr-xr-x 9,734 bytes parent folder | download | duplicates (2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
package jgi;

import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.HashSet;

import dna.Data;
import fileIO.ByteFile1;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextStreamWriter;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;

/**
 * Grab reads with specified numbers from a file.
 * TODO Note that much of this is ripped directly from ReformatReads, but is incorrect, because this class does not support dual output files.
 * @author Brian Bushnell
 * @date Jul 10, 2013
 *
 */
public class GetReads {
	
	public static void main(String[] args){
		GetReads x=new GetReads(args);
		
		//Close the print stream if it was redirected
		Shared.closeStream(x.outstream);
	}
	
	public GetReads(String[] args){
		
		{//Preparse block for help, config files, and outstream
			PreParser pp=new PreParser(args, getClass(), false);
			args=pp.args;
			outstream=pp.outstream;
		}
		
		Timer t=new Timer();

		Parser parser=new Parser();
		String in1=null;
		String in2=null;
		
		String qfin1=null;
		String qfin2=null;

		String out1=null;
		String out2=null;

		String qfout1=null;
		String qfout2=null;
		
		boolean errorState=false;
		long maxReads=-1;
		int passes=1;
		boolean testsize=false;
		boolean overwrite=false, append=false;
		float samplerate=1f;
		long sampleseed=1;

		
		
		ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
		
		HashSet<Long> table=new HashSet<Long>();
		
		for(int i=0; i<args.length; i++){
			String arg=args[i];
			String[] split=arg.split("=");
			String a=split[0].toLowerCase();
			String b=split.length>1 ? split[1] : null;
			
			if(Parser.parseCommonStatic(arg, a, b)){
				//do nothing
			}else if(Parser.parseZip(arg, a, b)){
				//do nothing
			}else if(Parser.parseQuality(arg, a, b)){
				//do nothing
			}else if(Parser.parseFasta(arg, a, b)){
				//do nothing
			}else if(parser.parseInterleaved(arg, a, b)){
				//do nothing
			}else if(a.equals("id") || a.equals("number")){
				assert(b!=null) : "Bad parameter: "+arg;
				String[] b2=b.split(",");
				for(String c : b2){
					final long x, y;
					if(c.indexOf('-')>=0){
						String[] c2=c.split("-");
						assert(c2.length==2) : c;
						x=Long.parseLong(c2[0]);
						y=Long.parseLong(c2[1]);
					}else{
						x=y=Long.parseLong(c);
					}
					for(long z=x; z<=y; z++){
						table.add(z);
					}
				}
			}else if(a.equals("passes")){
				passes=Integer.parseInt(b);
			}else if(a.equals("verbose")){
				verbose=Parse.parseBoolean(b);
				ByteFile1.verbose=verbose;
				stream.FastaReadInputStream.verbose=verbose;
			}else if(a.equals("reads") || a.startsWith("maxreads")){
				maxReads=Parse.parseKMG(b);
			}else if(a.equals("build") || a.equals("genome")){
				Data.setGenome(Integer.parseInt(b));
			}else if(a.equals("in") || a.equals("input") || a.equals("in1") || a.equals("input1")){
				assert(b!=null) : "Bad parameter: "+arg;
				in1=b;
				if(b.indexOf('#')>-1 && !new File(b).exists()){
					in1=b.replace("#", "1");
					in2=b.replace("#", "2");
				}
			}else if(a.equals("in2") || a.equals("input2")){
				in2=b;
			}else if(a.equals("out") || a.equals("output") || a.equals("out1") || a.equals("output1")){
				assert(b!=null) : "Bad parameter: "+arg;
				out1=b;
				if(b.indexOf('#')>-1){
					out1=b.replace("#", "1");
					out2=b.replace("#", "2");
				}
			}else if(a.equals("out2") || a.equals("output2")){
				out2=b;
			}else if(a.equals("qfin") || a.equals("qfin1")){
				qfin1=b;
			}else if(a.equals("qfout") || a.equals("qfout1")){
				qfout1=b;
			}else if(a.equals("qfin2")){
				qfin2=b;
			}else if(a.equals("qfout2")){
				qfout2=b;
			}else if(a.equals("testsize")){
				testsize=Parse.parseBoolean(b);
			}else if(a.equals("append") || a.equals("app")){
				append=ReadStats.append=Parse.parseBoolean(b);
			}else if(a.equals("overwrite") || a.equals("ow")){
				overwrite=Parse.parseBoolean(b);
			}else if(a.equals("samplerate")){
				samplerate=Float.parseFloat(b);
				assert(samplerate<=1f && samplerate>=0f) : "samplerate="+samplerate+"; should be between 0 and 1";
			}else if(a.equals("sampleseed")){
				sampleseed=Long.parseLong(b);
			}else if(a.startsWith("minscaf") || a.startsWith("mincontig")){
				stream.FastaReadInputStream.MIN_READ_LEN=Integer.parseInt(b);
			}else if(in1==null && i==0 && Tools.looksLikeInputStream(arg)){
				in1=arg;
				if(arg.indexOf('#')>-1 && !new File(arg).exists()){
					in1=arg.replace("#", "1");
					in2=arg.replace("#", "2");
				}
			}else{
				System.err.println("Unknown parameter "+args[i]);
				assert(false) : "Unknown parameter "+args[i];
				//				throw new RuntimeException("Unknown parameter "+args[i]);
			}
		}
		
		{//Process parser fields
			Parser.processQuality();
		}
		
		assert(FastaReadInputStream.settingsOK());
		
		if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
		
		if(out1==null){
			if(out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
			out1="stdout";
		}
		
		if(!parser.setInterleaved){
			assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
			if(in2!=null){ //If there are 2 input streams.
				FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
				outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
			}else{ //There is one input stream.
				if(out2!=null){
					FASTQ.FORCE_INTERLEAVED=true;
					FASTQ.TEST_INTERLEAVED=false;
					outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
				}
			}
		}

		if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
		if(out2!=null && out2.equalsIgnoreCase("null")){out2=null;}
		
		if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
			throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
		}
		

		FileFormat ffin=FileFormat.testInput(in1, 0, null, true, true);
		FileFormat ffout=FileFormat.testOutput(out1, 0, null, true, overwrite, append, false);
		
		
		final boolean useSharedHeader=(ffin!=null && ffout!=null && ffin.samOrBam() && ffout.samOrBam());
		
		if(ffin!=null && ffout!=null && ffin.samOrBam() && (ffout.samOrBam() || ffout.bread())){
			throw new RuntimeException("\nDirect conversion of sam to sam or bread are not currently supported.\nAll other conversions are possible.");
		}
		
		
		ConcurrentReadInputStream cris;
		{
			FileFormat ff1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
			FileFormat ff2=FileFormat.testInput(in2, FileFormat.FASTQ, null, true, true);
			cris=ConcurrentReadInputStream.getReadInputStream(maxReads, useSharedHeader, ff1, ff2);
		}
		
		cris.setSampleRate(samplerate, sampleseed);
		outstream.println("Input is "+(cris.paired() ? "paired" : "unpaired"));
		cris.start(); //4567

		TextStreamWriter tsw=new TextStreamWriter(out1, overwrite, false, false);
		tsw.start();
		
		
		long readsProcessed=0;
		long basesProcessed=0;

		for(int pass=1; pass<=passes; pass++){
//			outstream.println("pass="+pass);
			if(pass>1){
				FileFormat ff1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
				FileFormat ff2=FileFormat.testInput(in2, FileFormat.FASTQ, null, true, true);
				cris=ConcurrentReadInputStream.getReadInputStream(maxReads, useSharedHeader, ff1, ff2);
				cris.setSampleRate(samplerate, sampleseed);
				cris.start();
			}
			
			ListNum<Read> ln=cris.nextList();
			ArrayList<Read> reads=(ln!=null ? ln.list : null);

			if(reads!=null && !reads.isEmpty()){
				Read r=reads.get(0);
				assert(ffin==null || ffin.samOrBam() || (r.mate!=null)==cris.paired());//ffin cannot be null
			}

			while(reads!=null && reads.size()>0 && !table.isEmpty() && ln!=null){//ln!=null is implied

				for(Read r1 : reads){
					{
						readsProcessed++;
						basesProcessed+=r1.length();
					}
					Read r2=r1.mate;
					if(r2!=null){
						readsProcessed++;
						basesProcessed+=r2.length();
					}
					
					if(table.remove(r1.numericID)){
						tsw.println(r1);
						if(r2!=null){tsw.println(r2);}
						if(table.isEmpty()){break;}
					}
				}

				cris.returnList(ln);
				ln=cris.nextList();
				reads=(ln!=null ? ln.list : null);
			}
			cris.returnList(ln);
			errorState|=ReadWrite.closeStream(cris);
		}

		if(tsw!=null){
			tsw.poisonAndWait();
		}
		
		errorState|=(cris.errorState());
		
		t.stop();
		outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
		
		if(testsize){
			long bytesProcessed=(new File(in1).length()+(in2==null ? 0 : new File(in2).length()))*passes;
			double xpnano=bytesProcessed/(double)(t.elapsed);
			String xpstring=(bytesProcessed<100000 ? ""+bytesProcessed : bytesProcessed<100000000 ? (bytesProcessed/1000)+"k" : (bytesProcessed/1000000)+"m");
			while(xpstring.length()<8){xpstring=" "+xpstring;}
			outstream.println("Bytes Processed:    "+xpstring+" \t"+Tools.format("%.2fm bytes/sec", xpnano*1000));
		}
		
		if(errorState){
			throw new RuntimeException("GetReads terminated in an error state; the output may be corrupt.");
		}
		
	}

	private PrintStream outstream=System.err;
	public static boolean verbose=false;
	
}