1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311
|
package jgi;
import java.util.ArrayList;
import align2.BandedAligner;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextStreamWriter;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentCollectionReadInputStream;
import stream.ConcurrentReadInputStream;
import stream.FASTQ;
import stream.Read;
import structures.ListNum;
/**
* Calculates an all-to-all identity matrix.
* @author Brian Bushnell
* @date Nov 23, 2014
*
*/
public class IdentityMatrix {
public static void main(String[] args){
Timer t=new Timer();
IdentityMatrix x=new IdentityMatrix(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public IdentityMatrix(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
FileFormat.PRINT_WARNING=false;
int maxEdits_=-1;
int maxWidth_=-1;
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){
//do nothing
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(a.equals("edits") || a.equals("maxedits")){
maxEdits_=Integer.parseInt(b);
}else if(a.equals("width") || a.equals("maxwidth")){
maxWidth_=Integer.parseInt(b);
}else if(a.equals("percent")){
percent=Parse.parseBoolean(b);
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
in1=parser.in1;
out1=parser.out1;
}
FASTQ.FORCE_INTERLEAVED=false;
FASTQ.TEST_INTERLEAVED=false;
maxEdits=maxEdits_==-1 ? BandedAligner.big : maxEdits_;
maxWidth=maxWidth_==-1 ? (int)(Tools.min(maxEdits, BandedAligner.big)*2L+1) : maxWidth_;
ffout1=FileFormat.testOutput(out1, FileFormat.TXT, null, true, true, false, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
}
void process(Timer t){
allReads=load();
Shared.setBufferLen(4);
ConcurrentCollectionReadInputStream cris=new ConcurrentCollectionReadInputStream(allReads, null, -1);
cris.start(); //4567
ArrayList<ProcessThread> threads=new ArrayList<ProcessThread>();
final int tmax=Tools.max(Shared.threads(), 1);
for(int i=0; i<tmax; i++){
threads.add(new ProcessThread(cris));
}
for(ProcessThread pt : threads){pt.start();}
for(ProcessThread pt : threads){
while(pt.getState()!=Thread.State.TERMINATED){
try {
pt.join();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
}
ReadWrite.closeStreams(cris);
final int numReads=allReads.size();
for(int i=1; i<numReads; i++){
Read r1=allReads.get(i);
assert(r1.numericID==i);
for(int j=0; j<i; j++){
Read r2=allReads.get(j);
assert(r2.numericID==j);
((float[])r2.obj)[i]=((float[])r1.obj)[j];
}
}
if(ffout1!=null){
TextStreamWriter tsw=new TextStreamWriter(ffout1);
tsw.start();
for(Read r : allReads){
float[] obj=(float[])r.obj;
tsw.print(r.id);
if(percent){
for(float f : obj){
tsw.print(Tools.format("\t%.2f", f));
}
}else{
for(float f : obj){
tsw.print(Tools.format("\t%.4f", f));
}
}
tsw.print("\n");
r.obj=null;
}
tsw.poisonAndWait();
}
t.stop();
outstream.println("Total Time: \t"+t);
outstream.println("Reads Processed: "+allReads.size()+" \t"+Tools.format("%.2fk alignments/sec", (allReads.size()*(long)(allReads.size())/(double)(t.elapsed))*1000000));
outstream.println("Min Similarity: "+Tools.format("%.5f", minID));
outstream.println("Max Similarity: "+Tools.format("%.5f", maxID));
outstream.println("Avg Similarity: "+Tools.format("%.5f", avgID));
}
private ArrayList<Read> load(){
Timer t=new Timer();
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, null);
if(verbose){outstream.println("Started cris");}
cris.start(); //4567
}
boolean paired=cris.paired();
assert(!paired) : "This program is not designed for paired reads.";
long readsProcessed=0;
int maxLen=0;
ArrayList<Read> bigList=new ArrayList<Read>();
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
bigList.add(r1);
maxLen=Tools.max(maxLen, r1.length());
readsProcessed++;
}
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
ReadWrite.closeStreams(cris);
if(verbose){outstream.println("Finished loading "+readsProcessed+" sequences.");}
longestSequence=maxLen;
t.stop();
outstream.println("Load Time: \t"+t);
return bigList;
}
/*--------------------------------------------------------------*/
private class ProcessThread extends Thread {
ProcessThread(ConcurrentReadInputStream cris_){
cris=cris_;
maxEdits2=Tools.min(maxEdits, longestSequence);
int width=Tools.min(maxEdits2*2+1, maxWidth);
bandy=BandedAligner.makeBandedAligner(width);
}
@Override
public void run(){
final int numReads=allReads.size();
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
double sum=0;
long compares=0;
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
float[] obj=new float[numReads];
r1.obj=obj;
for(Read r2 : allReads){
if(r2.numericID>r1.numericID){break;}
// int edits=bandy.alignQuadruple(r1.bases, r2.bases, maxEdits2, false);
int edits=bandy.alignQuadrupleProgressive(r1.bases, r2.bases, 10, maxEdits2, false);
System.err.println(r1.id+"->"+r2.id+": Edits="+edits);
float editRate=edits/(float)Tools.max(r1.length(), r2.length());
float similarity=1-editRate;
if(r1!=r2){
compares++;
sum+=similarity;
minID=Tools.min(minID, similarity);
maxID=Tools.max(maxID, similarity);
}
if(percent){
float id=100*similarity;
obj[(int)r2.numericID]=id;
}else{
obj[(int)r2.numericID]=similarity;
}
}
}
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
avgID=sum/compares;
}
private final ConcurrentReadInputStream cris;
private final BandedAligner bandy;
private final int maxEdits2;
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
private String in1=null;
private String out1=null;
private final FileFormat ffin1;
private final FileFormat ffout1;
private boolean percent=false;
private ArrayList<Read> allReads;
/*--------------------------------------------------------------*/
private long maxReads=-1;
private final int maxEdits;
private final int maxWidth;
private int longestSequence;
private double minID=1, maxID=0, avgID=0;
/*--------------------------------------------------------------*/
private java.io.PrintStream outstream=System.err;
public static boolean verbose=false;
}
|