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package jgi;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.PriorityQueue;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import stream.SamLine;
import structures.ListNum;
import tracker.ReadStats;
/**
* @author Brian Bushnell
* @date June 1, 2016
*
*/
public class PartitionReads {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer();
PartitionReads x=new PartitionReads(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public PartitionReads(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
boolean setInterleaved=false; //Whether interleaved was explicitly set.
//Set shared static variables
Shared.setBufferLen(Tools.max(400, Shared.bufferLen()));
Shared.capBuffers(2);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
SamLine.SET_FROM_OK=true;
//Create a parser object
Parser parser=new Parser();
String qfout1=null, qfout2=null;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("ways")){
ways=Integer.parseInt(b);
}else if(a.equals("splitbybp") || a.equals("bybp") || a.equals("bp")){
splitByBP=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("pacbio") || a.equals("subreads")){
pacBioMode=Parse.parseBoolean(b);
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
setInterleaved=parser.setInterleaved;
in1=parser.in1;
in2=parser.in2;
qfin1=parser.qfin1;
qfin2=parser.qfin2;
out1=parser.out1;
out2=parser.out2;
qfout1=parser.qfout1;
qfout2=parser.qfout2;
extin=parser.extin;
extout=parser.extout;
}
assert(ways>0) : "Ways must be at least 1.";
//Do input file # replacement
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
//Do qfin # replacement
if(qfin1!=null && qfin2==null && qfin1.indexOf('#')>-1 && !new File(qfin1).exists()){
qfin2=qfin1.replace("#", "2");
qfin1=qfin1.replace("#", "1");
}
//Do output file # replacement
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
//Do output file # replacement
if(qfout1!=null && qfout2==null && qfout1.indexOf('#')>-1){
qfout2=qfout1.replace("#", "2");
qfout1=qfout1.replace("#", "1");
}
//Adjust interleaved detection based on the number of input files
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){outstream.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
//Ensure there is an input file
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
//Ensure out2 is not set without out1
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
//Adjust interleaved settings based on number of output files
if(!setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}
}
}
assert(out1==null || out1.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
assert(out2==null || out2.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
assert(qfout1==null || qfout1.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
assert(qfout2==null || qfout2.contains("%")) : "Output filename must contain '%' symbol, which will be replaced by affix.";
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
outstream.println((out1==null)+", "+(out2==null)+", "+out1+", "+out2);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1, in2)){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, in2, out1, out2)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
//Create output FileFormat objects
if(out1==null){
ffout1=ffout2=null;
qfout1Array=qfout2Array=null;
}else{
ffout1=new FileFormat[ways];
ffout2=new FileFormat[ways];
qfout1Array=new String[ways];
qfout2Array=new String[ways];
for(int i=0; i<ways; i++){
String temp1=out1==null ? null : out1.replaceFirst("%", ""+i);
String temp2=out2==null ? null : out2.replaceFirst("%", ""+i);
ffout1[i]=FileFormat.testOutput(temp1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2[i]=FileFormat.testOutput(temp2, FileFormat.FASTQ, extout, true, overwrite, append, false);
qfout1Array[i]=qfout1==null ? null : qfout1.replaceFirst("%", ""+i);
qfout2Array[i]=qfout2==null ? null : qfout2.replaceFirst("%", ""+i);
}
}
//Create input FileFormat objects
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
useSharedHeader=(ffin1.samOrBam() && ffout1!=null && ffout1.length>0 && ffout1[0]!=null && ffout1[0].samOrBam());
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Create a read input stream
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2, qfin1, qfin2);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
}
boolean paired=cris.paired();
if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
//Optionally create a read output stream
final ConcurrentReadOutputStream ros[];
if(ffout1!=null && ffout1.length>0){
ros=new ConcurrentReadOutputStream[ways];
final int buff=1;
for(int i=0; i<ways; i++){
ros[i]=ConcurrentReadOutputStream.getStream(ffout1[i], ffout2[i], qfout1Array[i], qfout2Array[i], buff, null, useSharedHeader);
ros[i].start(); //Start the stream
}
}else{ros=null;}
//Reset counters
readsProcessed=0;
basesProcessed=0;
//Process the read stream
if(!splitByBP) {processInner(cris, ros);}
else {processInner_heap(cris, ros);}
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Close the read streams
errorState|=ReadWrite.closeStreams(cris, ros);
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/** Iterate through the reads */
void processInner(final ConcurrentReadInputStream cris, final ConcurrentReadOutputStream ros[]){
//Do anything necessary prior to processing
{
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//Grab the actual read list from the ListNum
ArrayList<Read> reads=(ln!=null ? ln.list : null);
//Check to ensure pairing is as expected
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
ArrayList<Read>[] outLists=new ArrayList[ways];
for(int i=0; i<ways; i++){
outLists[i]=new ArrayList<Read>();
}
int nextIndex=0;
//As long as there is a nonempty read list...
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
//Loop through each read in the list
for(Read r1 : reads){
final Read r2=r1.mate;
//Track the initial length for statistics
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
//Increment counters
readsProcessed+=r1.pairCount();
basesProcessed+=initialLength1+initialLength2;
if(pacBioMode){
int zmw=Parse.parseZmw(r1.id);
assert(zmw>=0) : "Invalid zmw in "+r1.id;
nextIndex=(zmw%ways);
}
outLists[nextIndex].add(r1);
nextIndex=(nextIndex+1)%ways;
}
//Output reads to the output stream
for(int i=0; i<ways; i++){
if(ros!=null){ros[i].add(outLists[i], ln.id);}
outLists[i]=new ArrayList<Read>();
}
//Notify the input stream that the list was used
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
//Fetch a new list
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
//Do anything necessary after processing
}
/** Iterate through the reads; outputs data trying to keep an equal number of bp per file. */
void processInner_heap(final ConcurrentReadInputStream cris, final ConcurrentReadOutputStream ros[]){
//Do anything necessary prior to processing
PriorityQueue<Partition> queue=new PriorityQueue<Partition>(ways);
for(int i=0; i<ways; i++){
queue.add(new Partition(i));
}
{
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//Grab the actual read list from the ListNum
ArrayList<Read> reads=(ln!=null ? ln.list : null);
//Check to ensure pairing is as expected
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
ArrayList<Read>[] outLists=new ArrayList[ways];
for(int i=0; i<ways; i++){
outLists[i]=new ArrayList<Read>();
}
//As long as there is a nonempty read list...
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
//Loop through each read in the list
for(Read r1 : reads){
final Read r2=r1.mate;
//Track the initial length for statistics
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
//Increment counters
readsProcessed+=r1.pairCount();
basesProcessed+=initialLength1+initialLength2;
Partition p=queue.poll();
outLists[p.id].add(r1);
p.bp+=r1.pairLength();
queue.add(p);
}
//Output reads to the output stream
for(int i=0; i<ways; i++){
if(ros!=null){ros[i].add(outLists[i], ln.id);}
outLists[i]=new ArrayList<Read>();
}
//Notify the input stream that the list was used
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
//Fetch a new list
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
//Do anything necessary after processing
}
private static class Partition implements Comparable<Partition> {
public Partition(final int id_) {id=id_;}
@Override
public int compareTo(Partition o) {
long dif=bp-o.bp;
return (dif>0 ? 1 : dif<0 ? -1 : id-o.id);
}
public final int id;
public long bp=0;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file path */
private String in1=null;
/** Secondary input file path */
private String in2=null;
private String qfin1=null;
private String qfin2=null;
/** Primary output file path */
private String out1=null;
/** Secondary output file path */
private String out2=null;
private String[] qfout1Array=null;
private String[] qfout2Array=null;
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
/** Split data into this many output files */
private int ways=-1;
/** Keep PacBio subreads together in the same file */
private boolean pacBioMode=false;
/** Optimize for similar numbers of bp instead of sequences */
private boolean splitByBP=false;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file */
private final FileFormat ffin1;
/** Secondary input file */
private final FileFormat ffin2;
/** Primary output file */
private final FileFormat[] ffout1;
/** Secondary output file */
private final FileFormat[] ffout2;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
private boolean useSharedHeader=false;
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
/** This flag has no effect on singlethreaded programs */
private final boolean ordered=false;
}
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