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package jgi;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.regex.Pattern;
import dna.Data;
import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import hiseq.IlluminaHeaderParser2;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.ListNum;
import structures.Quantizer;
import tracker.ReadStats;
/**
* @author Brian Bushnell
* @date Aug 23, 2013
*
*/
public class RenameReads {
public static void main(String[] args){
Timer t=new Timer();
RenameReads x=new RenameReads(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public RenameReads(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Parser parser=new Parser();
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=Parse.splitOnFirst(arg, '=');
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(parser.parseInterleaved(arg, a, b)){
//do nothing
}else if(a.equals("passes")){
assert(false) : "'passes' is disabled.";
// passes=Integer.parseInt(b);
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
ByteFile2.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
ConcurrentGenericReadInputStream.verbose=verbose;
stream.FastqReadInputStream.verbose=verbose;
ReadWrite.verbose=verbose;
}else if(a.equalsIgnoreCase("fixSRA")){
fixSRA=Parse.parseBoolean(b);
}else if(a.equals("reads") || a.equals("maxreads")){
maxReads=Parse.parseKMG(b);
}else if(a.equals("build") || a.equals("genome")){
Data.setGenome(Integer.parseInt(b));
}else if(a.equals("in") || a.equals("input") || a.equals("in1") || a.equals("input1")){
in1=b;
}else if(a.equals("prefix") || a.equals("p")){
prefix=b;
}else if(a.equals("suffix")){
suffix=b;
}else if(a.equals("in2") || a.equals("input2")){
in2=b;
}else if(a.equals("out") || a.equals("output") || a.equals("out1") || a.equals("output1")){
out1=b;
}else if(a.equals("out2") || a.equals("output2")){
out2=b;
}else if(a.equals("qfin") || a.equals("qfin1")){
qfin1=b;
}else if(a.equals("qfout") || a.equals("qfout1")){
qfout1=b;
}else if(a.equals("qfin2")){
qfin2=b;
}else if(a.equals("qfout2")){
qfout2=b;
}else if(a.equals("extin")){
extin=b;
}else if(a.equals("extout")){
extout=b;
}else if(a.equals("trimright")){
trimRight=Integer.parseInt(b);
}else if(a.equals("trimleft")){
trimLeft=Integer.parseInt(b);
}else if(a.equals("trimbeforesymbol")){
trimBeforeSymbol=Integer.parseInt(b);
}else if(a.equals("symbol")){
String s=Parse.parseSymbol(b);
assert(s.length()==1) : "'"+s+"'";
symbol=s.charAt(0);
}
else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
}else if(a.equals("renamebyinsert")){
renameByInsert=Parse.parseBoolean(b);
}else if(a.equals("renamebytrim")){
renameByTrim=Parse.parseBoolean(b);
}else if(a.equals("renamebycoordinates") || a.equals("coordinates") || a.equals("coords")){
renameByCoords=Parse.parseBoolean(b);
}else if(a.equals("quantize") || a.equals("quantizesticky")){
quantizeQuality=Quantizer.parse(arg, a, b);
}else if(a.equals("addprefix")){
addPrefix=Parse.parseBoolean(b);
}else if(a.equals("addpairnum")){
addPairnum=Parse.parseBoolean(b);
}else if(a.equals("prefixonly")){
prefixOnly=Parse.parseBoolean(b);
}else if(a.equals("underscore") || a.equals("addunderscore")){
addUnderscore=Parse.parseBoolean(b);
}else if(a.startsWith("minscaf") || a.startsWith("mincontig")){
stream.FastaReadInputStream.MIN_READ_LEN=Integer.parseInt(b);
}else if(in1==null && i==0 && Tools.looksLikeInputStream(arg)){
in1=arg;
}else{
System.err.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
renameByMapping=FASTQ.TAG_CUSTOM;
}
if(addUnderscore && prefix!=null && !prefix.endsWith("_") && !prefixOnly){prefix+="_";}
if(!addPairnum){pairnums=new String[] {"",""};}
{//Process parser fields
Parser.processQuality();
}
// assert(false) : prefix;
if(prefix==null || prefix.length()<1){prefix="";}
// else if(!prefix.endsWith("_") && !prefixOnly){
// prefix=prefix+"_";
// }
if(renameByInsert){
prefix="insert=";
FASTQ.PARSE_CUSTOM=true;
}else if(renameByTrim){
prefix="";
FASTQ.PARSE_CUSTOM=true;
}
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){System.err.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
if(!parser.setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}
}
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(out2!=null && out2.equalsIgnoreCase("null")){out2=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
if(renameByMapping){
assert(ffout1==null || ffout1.fastq()) : "Currently renameByMapping requires fastq output.";
}
}
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2, qfin1, qfin2);
cris.start(); //4567
}
// TextStreamWriter tsw=new TextStreamWriter(args[2], false, false, true);
// tsw.start();
ConcurrentReadOutputStream ros=null;
if(out1!=null){
final int buff=4;
if(cris.paired() && out2==null && (in1==null || !in1.contains(".sam"))){
outstream.println("Writing interleaved.");
}
assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
assert(out2==null || (!out2.equalsIgnoreCase(in1) && !out2.equalsIgnoreCase(in2))) : "out1 and out2 have same name.";
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, qfout1, qfout2, buff, null, false);
ros.start();
}
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
IlluminaHeaderParser2 ihp=new IlluminaHeaderParser2();
ByteBuilder bb=new ByteBuilder();
long x=0;
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
for(Read r1 : reads){
final Read r2=r1.mate;
// assert(false) : trimBeforeSymbol+", '"+symbol+"'";
if(quantizeQuality){
final byte[] quals1=r1.quality, quals2=(r2==null ? null : r2.quality);
Quantizer.quantize(quals1);
Quantizer.quantize(quals2);
}
if(trimRight>0 || trimLeft>0 || trimBeforeSymbol>0) {
if(trimRight>0 || trimLeft>0){
r1.id=trim(r1.id, trimLeft, trimRight);
if(r2!=null) {r2.id=trim(r2.id, trimLeft, trimRight);}
}
if(trimBeforeSymbol>0){
r1.id=trimBeforeSymbol(r1.id, trimBeforeSymbol, symbol);
if(r2!=null) {r2.id=trimBeforeSymbol(r2.id, trimBeforeSymbol, symbol);}
}
}else if(fixSRA){
fixSRA(r1);
fixSRA(r2);
}else if(renameByCoords){
bb.clear().colon().colon().colon();
ihp.parse(r1);
ihp.appendCoordinates(bb).space().append(1).colon();
r1.id=bb.toString();
if(r2!=null) {
bb.set(bb.length-2, (byte)'2');
r2.id=bb.toString();
}
}else if(renameByMapping){
//Should be handled automatically, if output is fastq.
}else if(r2!=null && (renameByInsert || renameByTrim)){
r1.setMapped(true);
r2.setMapped(true);
x=Read.insertSizeMapped(r1, r2, false);
if(verbose){System.err.println("True Insert: "+x);}
if(renameByTrim){
r1.id=r1.numericID+"_"+r1.length()+"_"+Tools.min(x, r1.length())+pairnums[0];
r2.id=r2.numericID+"_"+r2.length()+"_"+Tools.min(x, r2.length())+pairnums[1];
}else{
String s=prefix+x;
r1.id=s+(addPairnum ? " 1:"+r1.numericID : "");
if(r2!=null){
r2.id=s+(addPairnum ? " 2:"+r1.numericID : "");
}
}
}else if(prefixOnly){
r1.id=prefix;
if(r2!=null){
r2.id=prefix;
}
x++;
}else if(addPrefix){
r1.id=prefix+r1.id;
if(r2!=null){
r2.id=prefix+r2.id;
}
x++;
}else if(suffix!=null) {
r1.id+="\t"+suffix;
if(r2!=null) {r2.id+="\t"+suffix;}
}else{
r1.id=prefix+x;
if(r2!=null){
r1.id=r1.id+pairnums[0];
r2.id=prefix+x+pairnums[1];
}
x++;
}
}
if(ros!=null){ros.add(reads, ln.id);}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
cris.returnList(ln);
errorState|=ReadWrite.closeStreams(cris, ros);
t.stop();
System.err.println("Time: "+t);
}
private void fixSRA(Read r){
//SRR1726611.11001 HWI-ST797:117:D091UACXX:4:1101:21093:8249 length=101
if(r==null){return;}
String id=r.id;
String[] split=spacePattern.split(id);
assert(split.length==3) : "Unrecognized format: "+id;
assert(split.length>0 && split[0].indexOf(':')<0) : "Unrecognized format: "+id;
if(split.length>1){
r.id=split[1]+pairnums[r.pairnum()];
}
}
private static String trim(String s, int left, int right) {
assert(left>=0 && right>=0) : left+", "+right;
assert(left>0 || right>0) : left+", "+right;
int len=s.length()-left-right;
if(len<0) {return "";}
else if(len>=s.length()) {return s;}
return s.substring(left, left+len);
}
private static String trimBeforeSymbol(String s, int right, char symbol) {
int pos=s.lastIndexOf(symbol);
// assert(false) : pos+", '"+symbol+"', "+right;
if(pos<0) {return s;}
int len=Tools.max(0, pos-right);
// assert(false) : pos+", "+len+", "+right;
String ret=s.substring(0, len)+s.substring(pos);
// assert(false) : pos+", "+len+", "+right+"\n"+s+"\n"+ret+"\n";
return ret;
}
private PrintStream outstream=System.err;
private String in1=null;
private String in2=null;
private String qfin1=null;
private String qfin2=null;
private String out1=null;
private String out2=null;
private String qfout1=null;
private String qfout2=null;
private String extin=null;
private String extout=null;
private String prefix=null;
private String suffix=null;
private final FileFormat ffin1;
private final FileFormat ffin2;
private final FileFormat ffout1;
private final FileFormat ffout2;
private boolean overwrite=true;
private boolean append=false;
private boolean verbose=false;
private long maxReads=-1;
public boolean errorState=false;
public int trimBeforeSymbol=0;
public char symbol;
public int trimRight=0;
public int trimLeft=0;
public boolean addUnderscore=true;
public boolean renameByMapping=false;
public boolean renameByInsert=false;
public boolean renameByTrim=false;
public boolean renameByCoords=false;
public boolean addPrefix=false;
public boolean prefixOnly=false;
public boolean fixSRA=false;
public boolean addPairnum=true;
public boolean quantizeQuality=false;
private String[] pairnums={" 1:", " 2:"};
private static final Pattern spacePattern=Pattern.compile("\\s+");
private static final Pattern whitespacePattern=Pattern.compile(" ");
}
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