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package jgi;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.KillSwitch;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* Replaces read headers with other headers.
*
* @author Brian Bushnell
* @date May 23, 2016
*
*/
public class ReplaceHeaders {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer();
ReplaceHeaders x=new ReplaceHeaders(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public ReplaceHeaders(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
boolean setInterleaved=false; //Whether interleaved was explicitly set.
//Set shared static variables
Shared.setBufferLen(1);//Tools.min(200, Shared.bufferLen());
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
//Create a parser object
Parser parser=new Parser();
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else if(a.equals("hin") || a.equals("hin1") || a.equals("inh") || a.equals("inh1") || a.equals("headerin") || a.equals("headerin1")){
hin1=b;
}else if(a.equals("hin2") || a.equals("inh2") || a.equals("headerin2")){
hin2=b;
}else if(a.equals("prefix") || a.equals("addprefix")){
prefix=Parse.parseBoolean(b);
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
setInterleaved=parser.setInterleaved;
in1=parser.in1;
in2=parser.in2;
qfin1=parser.qfin1;
qfin2=parser.qfin2;
out1=parser.out1;
out2=parser.out2;
qfout1=parser.qfout1;
qfout2=parser.qfout2;
extin=parser.extin;
extout=parser.extout;
}
//Do input file # replacement
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
//Do header input file # replacement
if(hin1!=null && hin2==null && hin1.indexOf('#')>-1 && !new File(hin1).exists()){
hin2=hin1.replace("#", "2");
hin1=hin1.replace("#", "1");
}
//Do output file # replacement
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
//Adjust interleaved detection based on the number of input files
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){outstream.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
//Ensure there is an input file
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
//Ensure there is an input file
if(hin1==null){throw new RuntimeException("Error - at least one header input file is required.");}
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
//Ensure out2 is not set without out1
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
//Adjust interleaved settings based on number of output files
if(!setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}
}
}
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
outstream.println((out1==null)+", "+(out2==null)+", "+out1+", "+out2);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1, in2, hin1, hin2)){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, in2, hin1, hin2, out1, out2)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
//Create output FileFormat objects
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, append, false);
//Create input FileFormat objects
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
//Create input FileFormat objects
ffhin1=FileFormat.testInput(hin1, FileFormat.HEADER, null, true, true);
ffhin2=FileFormat.testInput(hin2, FileFormat.HEADER, null, true, true);
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Create a read input stream
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2, qfin1, qfin2);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
}
final boolean paired=cris.paired();
if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
//Create a read input stream for headers
final ConcurrentReadInputStream hcris;
{
hcris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffhin1, ffhin2, null, null);
hcris.start(); //Start the stream
boolean paired2=hcris.paired();
if(verbose){outstream.println("Started hcris");}
if(paired!=paired2){
KillSwitch.kill("Reads and heades must agree on pairedness: reads="+paired+", headers="+paired2);
}
}
//Optionally create a read output stream
final ConcurrentReadOutputStream ros;
if(ffout1!=null){
final int buff=4;
if(cris.paired() && out2==null && (in1!=null && !ffin1.samOrBam() && !ffout1.samOrBam())){
outstream.println("Writing interleaved.");
}
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, qfout1, qfout2, buff, null, false);
ros.start(); //Start the stream
}else{ros=null;}
//Reset counters
readsProcessed=0;
basesProcessed=0;
//Process the read stream
processInner(cris, hcris, ros);
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Close the read streams
errorState|=ReadWrite.closeStreams(hcris);
errorState|=ReadWrite.closeStreams(cris, ros);
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/** Iterate through the reads */
void processInner(final ConcurrentReadInputStream cris, final ConcurrentReadInputStream hcris, final ConcurrentReadOutputStream ros){
//Do anything necessary prior to processing
{
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//Grab the actual read list from the ListNum
ArrayList<Read> reads=(ln!=null ? ln.list : null);
//Grab the first ListNum of headers
ListNum<Read> hln=hcris.nextList();
//Grab the actual read list from the ListNum
ArrayList<Read> hreads=(hln!=null ? hln.list : null);
if(hreads==null || hreads.size()!=reads.size()){
KillSwitch.kill("Wrong number of headers for the reads: "+reads.size()+"!="+hreads.size());
}
//Check to ensure pairing is as expected
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
Read h=hreads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
assert((r.mate!=null) == (h.mate!=null));
}
//As long as there is a nonempty read list...
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){
outstream.println("Fetched "+reads.size()+" reads: "+reads.get(0).id+"\n"+reads.get(0).length());
outstream.println("Fetched "+hreads.size()+" headers.");
}
//Loop through each read in the list
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
final Read r2=r1.mate;
final Read h1=hreads.get(idx);
final Read h2=h1.mate;
//Track the initial length for statistics
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
//Increment counters
readsProcessed+=r1.pairCount();
basesProcessed+=initialLength1+initialLength2;
boolean keep=processReadPair(r1, r2, h1, h2);
if(!keep){reads.set(idx, null);}
}
//Output reads to the output stream
if(ros!=null){ros.add(reads, ln.id);}
//Notify the input stream that the list was used
cris.returnList(ln);
if(verbose){outstream.println("Returned a read list.");}
//Notify the input stream that the list was used
hcris.returnList(hln.id, hln.list.isEmpty());
if(verbose){outstream.println("Returned a header list.");}
//Fetch a new list
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
//Fetch a new header list
hln=hcris.nextList();
hreads=(hln!=null ? hln.list : null);
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
//Notify the input stream that the final header list was used
if(hln!=null){
hcris.returnList(hln.id, hln.list==null || hln.list.isEmpty());
}
}
//Do anything necessary after processing
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
/**
* Process a single read pair.
* @param r1 Read 1
* @param r2 Read 2 (may be null)
* @return True if the reads should be kept, false if they should be discarded.
*/
boolean processReadPair(final Read r1, final Read r2, final Read h1, final Read h2){
if(prefix){
r1.id=h1.id+" "+r1.id;
if(r2!=null){r2.id=h2.id+" "+r2.id;}
}else{
r1.id=h1.id;
if(r2!=null){r2.id=h2.id;}
}
return true;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file path */
private String in1=null;
/** Secondary input file path */
private String in2=null;
/** Primary header input file path */
private String hin1=null;
/** Secondary header input file path */
private String hin2=null;
private String qfin1=null;
private String qfin2=null;
/** Primary output file path */
private String out1=null;
/** Secondary output file path */
private String out2=null;
private String qfout1=null;
private String qfout2=null;
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
/** Prepend the new name to the old name */
private boolean prefix=false;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file */
private final FileFormat ffin1;
/** Secondary input file */
private final FileFormat ffin2;
/** Primary header input file */
private final FileFormat ffhin1;
/** Secondary header input file */
private final FileFormat ffhin2;
/** Primary output file */
private final FileFormat ffout1;
/** Secondary output file */
private final FileFormat ffout2;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
}
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