1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304
|
#ifndef REF_READ_H_
#define REF_READ_H_
#include <iostream>
#include <cassert>
#include <vector>
#include <string>
#include <ctype.h>
#include <fstream>
#include <stdexcept>
#include <seqan/sequence.h>
#include "alphabet.h"
#include "assert_helpers.h"
#include "filebuf.h"
#include "word_io.h"
#include "endian_swap.h"
using namespace std;
using namespace seqan;
class RefTooLongException : public exception {
public:
RefTooLongException() {
#ifdef BOWTIE_64BIT_INDEX
// This should never happen!
msg = "Error: Reference sequence has more than 2^64-1 characters! "
"Please divide the reference into smaller chunks and index each "
"independently.";
#else
msg = "Error: Reference sequence has more than 2^32-1 characters! "
"Please build a large index by passing the --large-index option "
"to bowtie2-build";
#endif
}
~RefTooLongException() throw() {}
const char* what() const throw() {
return msg.c_str();
}
protected:
string msg;
};
/**
* Encapsulates a stretch of the reference containing only unambiguous
* characters. From an ordered list of RefRecords, one can (almost)
* deduce the "shape" of the reference sequences (almost because we
* lose information about stretches of ambiguous characters at the end
* of reference sequences).
*/
struct RefRecord {
RefRecord() : off(), len(), first() { }
RefRecord(TIndexOffU _off, TIndexOffU _len, bool _first) :
off(_off), len(_len), first(_first)
{ }
RefRecord(FILE *in, bool swap) {
assert(in != NULL);
if(!fread(&off, OFF_SIZE, 1, in)) {
cerr << "Error reading RefRecord offset from FILE" << endl;
throw 1;
}
if(swap) off = endianSwapU(off);
if(!fread(&len, OFF_SIZE, 1, in)) {
cerr << "Error reading RefRecord offset from FILE" << endl;
throw 1;
}
if(swap) len = endianSwapU(len);
first = fgetc(in) ? true : false;
}
void write(std::ostream& out, bool be) {
writeU<TIndexOffU>(out, off, be);
writeU<TIndexOffU>(out, len, be);
out.put(first ? 1 : 0);
}
TIndexOffU off; /// Offset of the first character in the record
TIndexOffU len; /// Length of the record
bool first; /// Whether this record is the first for a reference sequence
};
enum {
REF_READ_FORWARD = 0, // don't reverse reference sequence
REF_READ_REVERSE, // reverse entire reference sequence
REF_READ_REVERSE_EACH // reverse each unambiguous stretch of reference
};
/**
* Parameters governing treatment of references as they're read in.
*/
struct RefReadInParams {
RefReadInParams(bool col, int r, bool nsToA, bool bisulf) :
color(col), reverse(r), nsToAs(nsToA), bisulfite(bisulf) { }
// extract colors from reference
bool color;
// reverse each reference sequence before passing it along
int reverse;
// convert ambiguous characters to As
bool nsToAs;
// bisulfite-convert the reference
bool bisulfite;
};
extern RefRecord
fastaRefReadSize(
FileBuf& in,
const RefReadInParams& rparms,
bool first,
BitpairOutFileBuf* bpout = NULL);
extern std::pair<size_t, size_t>
fastaRefReadSizes(
vector<FileBuf*>& in,
vector<RefRecord>& recs,
vector<uint32_t>& plens,
const RefReadInParams& rparms,
BitpairOutFileBuf* bpout,
TIndexOff& numSeqs);
extern void
reverseRefRecords(
const vector<RefRecord>& src,
vector<RefRecord>& dst,
bool recursive = false,
bool verbose = false);
/**
* Reads the next sequence from the given FASTA file and appends it to
* the end of dst, optionally reversing it.
*/
template <typename TStr>
static RefRecord fastaRefReadAppend(FileBuf& in,
bool first,
TStr& dst,
RefReadInParams& rparms,
string* name = NULL)
{
typedef typename Value<TStr>::Type TVal;
int c;
static int lastc = '>';
if(first) {
c = in.getPastWhitespace();
if(c != '>') {
cerr << "Reference file does not seem to be a FASTA file" << endl;
throw 1;
}
lastc = c;
}
assert_neq(-1, lastc);
// RefRecord params
size_t len = 0;
size_t off = 0;
first = true;
size_t ilen = length(dst);
// Chew up the id line; if the next line is either
// another id line or a comment line, keep chewing
int lc = -1; // last-DNA char variable for color conversion
c = lastc;
if(c == '>' || c == '#') {
do {
while (c == '#') {
if((c = in.getPastNewline()) == -1) {
lastc = -1;
goto bail;
}
}
assert_eq('>', c);
while(true) {
c = in.get();
if(c == -1) {
lastc = -1;
goto bail;
}
if(c == '\n' || c == '\r') {
while(c == '\r' || c == '\n') c = in.get();
if(c == -1) {
lastc = -1;
goto bail;
}
break;
}
if (name) name->push_back(c);
}
// c holds the first character on the line after the name
// line
} while (c == '>' || c == '#');
} else {
ASSERT_ONLY(int cc = toupper(c));
assert(cc != 'A' && cc != 'C' && cc != 'G' && cc != 'T');
first = false;
}
// Skip over an initial stretch of gaps or ambiguous characters.
// For colorspace we skip until we see two consecutive unambiguous
// characters (i.e. the first unambiguous color).
while(true) {
int cat = dna4Cat[c];
if(rparms.nsToAs && cat == 2) {
c = 'A';
}
int cc = toupper(c);
if(rparms.bisulfite && cc == 'C') c = cc = 'T';
if(cat == 1) {
// This is a DNA character
if(rparms.color) {
if(lc != -1) {
// Got two consecutive unambiguous DNAs
break; // to read-in loop
}
// Keep going; we need two consecutive unambiguous DNAs
lc = charToDna5[(int)c];
// The 'if(off > 0)' takes care of the case where
// the reference is entirely unambiguous and we don't
// want to incorrectly increment off.
if(off > 0) off++;
} else {
break; // to read-in loop
}
} else if(cat == 2) {
if(lc != -1 && off == 0) {
off++;
}
lc = -1;
off++; // skip it
} else if(c == '>') {
lastc = '>';
goto bail;
}
c = in.get();
if(c == -1) {
lastc = -1;
goto bail;
}
}
if(first && rparms.color && off > 0) {
// Handle the case where the first record has ambiguous
// characters but we're in color space; one of those counts is
// spurious
off--;
}
assert(!rparms.color || lc != -1);
assert_eq(1, dna4Cat[c]);
// in now points just past the first character of a sequence
// line, and c holds the first character
while(true) {
// Note: can't have a comment in the middle of a sequence,
// though a comment can end a sequence
int cat = dna4Cat[c];
assert_neq(2, cat);
if(cat == 1) {
// Consume it
if(!rparms.color || lc != -1) len++;
// Add it to referenece buffer
if(rparms.color) {
appendValue(dst, (Dna)dinuc2color[charToDna5[(int)c]][lc]);
} else if(!rparms.color) {
appendValue(dst, (Dna)(char)c);
}
assert_lt((uint8_t)(Dna)dst[length(dst)-1], 4);
lc = charToDna5[(int)c];
}
c = in.get();
if(rparms.nsToAs && dna4Cat[c] == 2) c = 'A';
if (c == -1 || c == '>' || c == '#' || dna4Cat[c] == 2) {
lastc = c;
break;
}
if(rparms.bisulfite && toupper(c) == 'C') c = 'T';
}
bail:
// Optionally reverse the portion that we just appended.
// ilen = length of buffer before this last sequence was appended.
if(rparms.reverse == REF_READ_REVERSE_EACH) {
// Find limits of the portion we just appended
size_t nlen = length(dst);
assert_eq(nlen - ilen, len);
if(len > 0) {
size_t halfway = ilen + (len>>1);
// Reverse it in-place
for(size_t i = ilen; i < halfway; i++) {
size_t diff = i-ilen;
size_t j = nlen-diff-1;
TVal tmp = dst[i];
dst[i] = dst[j];
dst[j] = tmp;
}
}
}
return RefRecord((TIndexOffU)off, (TIndexOffU)len, first);
}
#endif /*ndef REF_READ_H_*/
|