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#include "stdafx.h"
/*
This file is a part of KMC software distributed under GNU GPL 3 licence.
The homepage of the KMC project is http://sun.aei.polsl.pl/kmc
Authors: Sebastian Deorowicz, Agnieszka Debudaj-Grabysz, Marek Kokot
Version: 3.1.1
Date : 2019-05-19
*/
#include <algorithm>
#include "defs.h"
#include "fastq_reader.h"
#include "bam_utils.h"
//************************************************************************************************************
// CFastqReader - reader class
//************************************************************************************************************
uint64 CFastqReader::OVERHEAD_SIZE = 1 << 16;
//----------------------------------------------------------------------------------
// Constructor of FASTA/FASTQ reader
// Parameters:
// * _mm - pointer to memory monitor (to check the memory limits)
CFastqReader::CFastqReader(CMemoryMonitor *_mm, CMemoryPoolWithBamSupport *_pmm_fastq, input_type _file_type, int _kmer_len,
CBinaryPackQueue* _binary_pack_queue, CMemoryPool* _pmm_binary_file_reader, CBamTaskManager* _bam_task_manager,
CPartQueue* _part_queue, CStatsPartQueue* _stats_part_queue, CMissingEOL_at_EOF_counter* _missingEOL_at_EOF_counter)
{
binary_pack_queue = _binary_pack_queue;
missingEOL_at_EOF_counter = _missingEOL_at_EOF_counter;
bam_task_manager = _bam_task_manager;
part_queue = _part_queue;
stats_part_queue = _stats_part_queue;
mm = _mm;
pmm_fastq = _pmm_fastq;
pmm_binary_file_reader = _pmm_binary_file_reader;
file_type = _file_type;
kmer_len = _kmer_len;
// Size and pointer for the buffer
part_size = 1 << 23;
part = nullptr;
containsNextChromosome = false;
data_src.SetQueue(binary_pack_queue, pmm_binary_file_reader);
}
//----------------------------------------------------------------------------------
// Destructor - close the files
CFastqReader::~CFastqReader()
{
if (part)
pmm_fastq->free(part);
}
//----------------------------------------------------------------------------------
// Set part size of the buffer
bool CFastqReader::SetPartSize(uint64 _part_size)
{
if (_part_size < (1 << 20) || _part_size >(1 << 30))
return false;
part_size = _part_size;
return true;
}
void CFastqReader::ProcessBamBinaryPart(uchar* data, uint64 size, uint32 id, uint32 file_no)
{
pmm_fastq->bam_reserve_gunzip(part, id);
part_filled = 0;
uint64_t inpos = 0;
while (inpos < size)
{
inpos += 16;
uint16_t BGZF_block_size_tmp;
read_uint16_t(BGZF_block_size_tmp, data, inpos);
uint64_t BGZF_block_size = BGZF_block_size_tmp + 1ull; //This integer gives the size of the containing BGZF block minus one.
uint64_t ISIZE_pos = inpos - 18 + BGZF_block_size - 4;
uint32_t ISIZE;
read_uint32_t(ISIZE, data, ISIZE_pos);
if (part_filled + ISIZE > part_size)
{
if (!bam_task_manager->PushGunzippedPart(part, part_filled, id, file_no))
{
pmm_fastq->free(part);
part = nullptr;
return;
}
uchar* prepare_for_splitter_data;
uint64 prepare_for_splitter_size;
uint32 prepare_for_splitter_id;
uint32 prepare_for_splitter_file_no;
while (bam_task_manager->TakeNextPrepareForSplitterTaskIfExists(prepare_for_splitter_data, prepare_for_splitter_size, prepare_for_splitter_id, prepare_for_splitter_file_no))
{
PreparePartForSplitter(prepare_for_splitter_data, prepare_for_splitter_size, prepare_for_splitter_id, prepare_for_splitter_file_no);
bam_task_manager->NotifySplitterPrepareTaskDone();
}
pmm_fastq->bam_reserve_gunzip(part, id);
part_filled = 0;
}
z_stream stream;
stream.zalloc = Z_NULL;
stream.zfree = Z_NULL;
stream.opaque = Z_NULL;
stream.avail_in = BGZF_block_size - 18;
stream.next_in = data + inpos;
stream.avail_out = part_size - part_filled;
stream.next_out = part + part_filled;
if (inflateInit2(&stream, -15) != Z_OK)
{
cerr << "Error: inflateInit2\n";
exit(1);
}
int ret = inflate(&stream, Z_NO_FLUSH);
switch (ret)
{
case Z_NEED_DICT:
ret = Z_DATA_ERROR; /* and fall through */
case Z_DATA_ERROR:
cerr << "Some error (Z_DATA_ERROR) while reading bam file, please contact authors, CODE: FastqReader_" << __LINE__ << "\n";
exit(1);
case Z_MEM_ERROR:
inflateEnd(&stream);
exit(ret);
}
if (ret == Z_STREAM_END)
inflateEnd(&stream);
else
{
cerr << "Error: Some error in bam file decompression, please contact authors, CODE: FastqReader_" << __LINE__ << "\n";
exit(1);
}
if (stream.avail_in != 8)
{
//cerr << "Err avail_in != 8:" << stream.avail_in << "\n";
cerr << "Error: Some error in bam file decompression, please contact authors, CODE: FastqReader_" << __LINE__ << "\n";
exit(1);
}
part_filled += ISIZE;
inpos += BGZF_block_size_tmp - 18 + 1;
//TODO: check CRC? in the future I may want to use libdeflate for bam decompression and crc checks
uint64_t CRC_pos = inpos - 8;
uint32_t CRC;
read_uint32_t(CRC, data, CRC_pos);
uLong crc = crc32(0L, Z_NULL, 0);
crc = crc32(crc, part + part_filled - ISIZE, ISIZE);
if (crc != CRC)
{
cerr << "Error: BGZF CRC check error. Input file may be corrupted\n";
exit(1);
}
}
if (!bam_task_manager->PushGunzippedPart(part, part_filled, id, file_no))
pmm_fastq->free(part);
part = nullptr;
}
void CFastqReader::PreparePartForSplitter(uchar* data, uint64 size, uint32 /*id*/, uint32 file_no)
{
auto& state = bam_task_manager->splitter_prepare_state;
uint64_t bpos = 0;
//if first in file, skip header
if (file_no != state.current_file_no)
{
state.current_file_no = file_no;
if (strncmp((const char*)data, "BAM\1", 4) != 0)
{
cerr << "BAM\\1 magic string missed\n";
exit(1);
}
int32_t l_text = 0;
bpos = 4;
read_int32_t(l_text, data, bpos);
bpos += l_text;
int32_t n_ref = 0;
read_int32_t(n_ref, data, bpos);
for (int32_t i = 0; i < n_ref; ++i)
{
int32_t l_name = 0;
read_int32_t(l_name, data, bpos);
bpos += l_name;
bpos += 4;
}
uint64 skip_header_offset = bpos;
//if contain header, remove it
memmove(data, data + skip_header_offset, size - skip_header_offset);
size -= skip_header_offset;
skip_header_offset = 0;
bpos = 0;
}
if (state.prev_part_size)
{
//assure block size present in prev_part
if (state.prev_part_size < 4)
{
memcpy(state.prev_part_data + state.prev_part_size, data, 4 - state.prev_part_size);
bpos += 4 - state.prev_part_size;
state.prev_part_size += 4 - state.prev_part_size;
}
int32_t prev_block_size;
uint64_t prev_bpos = 0;
read_int32_t(prev_block_size, state.prev_part_data, prev_bpos);
uint64_t missing = prev_block_size - (state.prev_part_size - 4);
memcpy(state.prev_part_data + state.prev_part_size, data + bpos, missing);
state.prev_part_size += missing;
bpos += missing;
if (part_queue)
{
part_queue->push(state.prev_part_data, state.prev_part_size, ReadType::na);
}
else if (stats_part_queue)
{
if (!stats_part_queue->push(state.prev_part_data, state.prev_part_size, ReadType::na))
{
pmm_fastq->free(state.prev_part_data);
pmm_fastq->free(data);
state.prev_part_data = nullptr;
state.prev_part_size = 0;
bam_task_manager->IgnoreRest(pmm_fastq, pmm_binary_file_reader);
return;
}
}
else
{
cerr << "Error: Should never be here, please contact authors, CODE: FastqReader_" << __LINE__ << "\n";
exit(1);
}
//TODO: this memmove could be avoided, the part queue could store starting pos, apropriate changes will be required in splitters
//The amount of moved data here may be quite large (close to size)
//on the other hand bam records should rare span multiple bgzf blocks and in case of newer BAM files it should not happen
//so for now I will let it be this way
memmove(data, data + bpos, size - bpos);
size -= bpos;
bpos = 0;
state.prev_part_data = nullptr;
state.prev_part_size = 0;
}
while (true)
{
if (bpos == size) // this pack contains full records, may be passed
{
if (part_queue)
{
part_queue->push(data, size, ReadType::na);
}
else if (stats_part_queue)
{
if (!stats_part_queue->push(data, size, ReadType::na))
{
pmm_fastq->free(data);
bam_task_manager->IgnoreRest(pmm_fastq, pmm_binary_file_reader);
return;
}
}
else
{
cerr << "Error: Should never be here, please contact authors, CODE: FastqReader_" << __LINE__ << "\n";
exit(1);
}
break;
}
bool span_multiple_bgzf_blocks = false;
int32_t block_size;
if (bpos + 4 > size) //part of block_size is in the next gunzipped part
{
span_multiple_bgzf_blocks = true;
}
else
{
read_int32_t(block_size, data, bpos);
if (bpos + block_size > size)
{
span_multiple_bgzf_blocks = true;
bpos -= 4;
}
}
if (span_multiple_bgzf_blocks)
{
pmm_fastq->reserve(state.prev_part_data);
memcpy(state.prev_part_data, data + bpos, size - bpos);
state.prev_part_size = size - bpos;
if (part_queue)
{
part_queue->push(data, bpos, ReadType::na);
}
else if (stats_part_queue)
{
if (!stats_part_queue->push(data, bpos, ReadType::na))
{
pmm_fastq->free(data);
pmm_fastq->free(state.prev_part_data);
state.prev_part_data = nullptr;
state.prev_part_size = 0;
bam_task_manager->IgnoreRest(pmm_fastq, pmm_binary_file_reader);
return;
}
}
else
{
cerr << "Error: Should never be here, please contact authors, CODE: FastqReader_" << __LINE__ << "\n";
exit(1);
}
break;
}
bpos += block_size;
}
}
//----------------------------------------------------------------------------------
// Read a part of the file in bam file format
void CFastqReader::ProcessBam()
{
CBamTaskManager::TaskType taskType;
uchar* data = nullptr;
uint64 size = 0;
uint32 id = (uint32)(-1);
uint32 file_no = (uint32)(-1);
while (bam_task_manager->PopTask(taskType, data, size, id, file_no))
{
if (taskType == CBamTaskManager::TaskType::Gunzip)
{
ProcessBamBinaryPart(data, size, id, file_no);
pmm_binary_file_reader->free(data);
bam_task_manager->NotifyIdFinished(id);
pmm_fastq->bam_notify_id_finished(id);
}
else if (taskType == CBamTaskManager::TaskType::PrepareForSplitter)
{
PreparePartForSplitter(data, size, id, file_no);
bam_task_manager->NotifySplitterPrepareTaskDone();
}
else
{
cerr << "Error: should never be here, plase contact authors, CODE: FastqReader_" << __LINE__ << "\n";
}
}
}
//----------------------------------------------------------------------------------
// Read a part of the file in multi line fasta format
bool CFastqReader::GetPartFromMultilneFasta(uchar *&_part, uint64 &_size)
{
uint64 readed = 0;
if (!containsNextChromosome)
{
if (data_src.Finished())
return false;
}
bool last_in_file;
readed = data_src.read(part + part_filled, (part_size - 1) - part_filled, last_in_file); //part_size - 1 to eventually append EOL if not present at EOF
int64 total_filled = part_filled + readed;
if (last_in_file)
FixEOLIfNeeded(part, total_filled);
int64 last_header_pos = 0;
int64 pos = 0;
for (int64 i = 0; i < total_filled; ++i)//find last '>' and remove EOLs
{
while (part[i] == '>') //issue 116, (if -> while) USE CASE: when there is empty seqence (only header, no data)
{
int64 tmp = i;
bool next_line = SkipNextEOL(part, i, total_filled);
if (!next_line)
i = total_filled;
copy(part + tmp, part + i, part + pos);
last_header_pos = pos;
pos += i - tmp;
if (!next_line)
break;
}
if (part[i] != '\n' && part[i] != '\r')
{
part[pos++] = part[i];
}
}
_part = part;
if (last_header_pos == 0)//data in block belong to one seq ('>' is the first symbol or do not occur at all)
{
part_filled = kmer_len - 1;
_size = pos;
if (_size < part_filled) //fixes 96
part_filled = 0;
pmm_fastq->reserve(part);
copy(_part + _size - part_filled, _part + _size, part);
containsNextChromosome = false;
}
else//next seq starts at last_header_pos
{
_size = last_header_pos;
part_filled = pos - last_header_pos;
pmm_fastq->reserve(part);
copy(_part + last_header_pos, _part + pos, part);
containsNextChromosome = true;
}
return true;
}
FORCE_INLINE void CFastqReader::FixEOLIfNeeded(uchar* part, int64& size)
{
uchar c = part[size - 1];
if (c != '\n' && c != '\r')
{
//std::cerr << "Warning: missing end of line character after last line in file\n";
missingEOL_at_EOF_counter->RegisterMissingEOL();
part[size++] = '\n'; //append fake EOL
}
}
FORCE_INLINE bool CFastqReader::GetNextSymbOfLongReadRecord(uchar& res, int64& p, int64& size)
{
if (p == size)
{
bool last_in_file;
size = data_src.read(part, part_size - 1, last_in_file);//part_size - 1 to eventually append EOL if not present at EOF
if(last_in_file)
FixEOLIfNeeded(part, size);
p = 0;
if (size == 0)
return false;
}
res = part[p++];
return true;
}
void CFastqReader::CleanUpAfterLongFastqRead(uint32 number_of_lines_to_skip)
{
pmm_fastq->reserve(part);
uchar symb;
int64 in_part = 0;
int64 skip_pos = 0;
uint32 state = 0; // 0 - skipping remaining EOLs, 1 - skipping line
while (GetNextSymbOfLongReadRecord(symb, skip_pos, in_part))
{
if (state == 0)
{
if (symb == '\n' || symb == '\r')
;
else
{
if (number_of_lines_to_skip)
state = 1;
else
{
if (symb != '@')
{
cerr << "Error: Wrong input file!\n";
exit(1);
}
std::copy(part + skip_pos - 1, part + in_part, part);
part_filled = in_part - (skip_pos - 1);
return;
}
}
}
else if (state == 1)
{
if (symb == '\n' || symb == '\r')
{
--number_of_lines_to_skip;
state = 0;
}
}
}
//the file has ended
part_filled = 0;
}
bool CFastqReader::GetPartNew(uchar *&_part, uint64 &_size, ReadType& read_type)
{
if (file_type == multiline_fasta)
{
read_type = ReadType::na;
return GetPartFromMultilneFasta(_part, _size);
}
if (data_src.Finished())
return false;
uint64 readed;
// Read data
bool last_in_file;
readed = data_src.read(part + part_filled, (part_size - 1) - part_filled, last_in_file);//part_size - 1 to eventually append EOL if not present at EOF
//std::cerr.write((char*)part + part_filled, readed);
int64 total_filled = part_filled + readed;
if (last_in_file)
FixEOLIfNeeded(part, total_filled);
int64 i;
if (data_src.Finished() && !long_read_in_progress)
{
_part = part;
_size = total_filled;
part = nullptr;
return true;
}
if (!total_filled) //better fix for issue 95
{
_part = part;
_size = 0;
} // Look for the end of the last complete record in a buffer
else if (file_type == fasta) // FASTA files
{
// Looking for a FASTA record at the end of the area
i = total_filled - 1;
int64 start, end;
int64 line_start[4], line_end[4];
int readed_lines = 0;
bool success = false;
int k;
while (i >= 0 && readed_lines < 4)
{
GetFullLineFromEnd(start, end, part, i);
line_start[4 - readed_lines - 1] = start;
line_end[4 - readed_lines - 1] = end;
++readed_lines;
if (readed_lines >= 2)
{
k = 4 - readed_lines;
if (part[line_start[k]] == '>')
{
success = true;
break;
}
}
}
// Looking for a FASTQ record at the end of the area
if (!success)
{
cerr << "Error: Wrong input file!\n";
exit(1);
}
_part = part;
_size = line_end[k + 1];
read_type = ReadType::normal_read;
}
else
{
if (long_read_in_progress)
{
//check if there is EOL in the data
int64 pos = 0;
for (; pos < total_filled; ++pos)
{
if (part[pos] == '\n' || part[pos] == '\r')
{
long_read_in_progress = false;
break;
}
}
if (!long_read_in_progress)
{
_part = part;
_size = pos;
//count the number of lines to skip after the part that is currently in the buffer (after read)
uint32 no_lines = 2;//qual header and qual
uint32 state = 0; //0 - skipping remaining EOLs, 1 - skipping line content
for (; pos < total_filled; ++pos)
{
if (state == 0)
{
if (part[pos] == '\n' || part[pos] == '\r')
state = 1;
}
else if (state == 1)
{
if (part[pos] == '\n' || part[pos] == '\r')
{
--no_lines;
state = 0;
}
}
}
CleanUpAfterLongFastqRead(no_lines);
}
else
{
_part = part;
_size = total_filled;
pmm_fastq->reserve(part);
std::copy(_part + total_filled - kmer_len + 1, _part + total_filled, part);
part_filled = kmer_len - 1;
}
return true;
}
i = total_filled - 1;
int64 start, end;
int64 line_start[8], line_end[8];
int readed_lines = 0;
bool success = false;
int k;
while (i >= 0 && readed_lines < 8)
{
GetFullLineFromEnd(start, end, part, i);
line_start[8 - readed_lines - 1] = start;
line_end[8 - readed_lines - 1] = end;
++readed_lines;
if (readed_lines >= 4)
{
k = 8 - readed_lines;
if (part[line_start[k]] == '@' && part[line_start[k + 2]] == '+')
{
if (part[line_start[k + 2] + 1] == '\n' || part[line_start[k + 2] + 1] == '\r')
{
success = true;
break;
}
if (line_start[k + 1] - line_start[k] == line_start[k + 3] - line_start[k + 2] &&
memcmp(part + line_start[k] + 1, part + line_start[k + 2] + 1, line_start[k + 3] - line_start[k + 2] - 1) == 0)
{
success = true;
break;
}
}
}
}
if (!success) //wrong input file or very long read
{
if (readed_lines == 4) //because if successfully reade full 4 lines in the worst case there is only one read that begins at the buffer start, and there is only onle fastq record
{
std::cerr << "Error: some error while reading fastq file, please contact authors\n";
exit(1);
}
k = 8 - readed_lines;
if (line_start[k] != 0)
{
std::cerr << "Error: some error while reading fastq file, please contact authors\n";
exit(1);
}
if (part[0] != '@')
{
cerr << "Error: Wrong input file!\n";
exit(1);
}
if (readed_lines == 1)
{
long_read_in_progress = true;
_part = part;
_size = total_filled;
read_type = ReadType::long_read;
//copy last k-1 symbols
pmm_fastq->reserve(part);
copy(_part + total_filled - kmer_len + 1, _part + total_filled, part);
part_filled = kmer_len - 1;
return true;
}
else //whole read had beed read
{
//readed_lines == 1 -> read header, and part of read
//readed_lines == 2 -> read header + read, and (possibly empty) part of qual header
//readed_lines == 3 -> read header + read + qual header, and (possibly empty) part of qual
//readed_lines == 4 -> should not be possible here
//so here only read_lines == 2 or 3 is possible
long_read_in_progress = false;
_part = part;
_size = line_end[k + 1];
read_type = ReadType::long_read;
//Some clean up to assure in next function call the buffer starts with @ or nothing (file finished)
uint32 number_of_lines_to_skip;
if (readed_lines == 2)
number_of_lines_to_skip = 2; // means inside qual header
else if (readed_lines == 3)
number_of_lines_to_skip = 1; //means inside qual
else //shold not be possible
{
std::cerr << "Error: some error while reading fastq file, please contact authors\n";
exit(1);
}
CleanUpAfterLongFastqRead(number_of_lines_to_skip);
return true;
}
}
_part = part;
_size = line_end[k + 3];
read_type = ReadType::normal_read;
}
// Allocate new memory for the buffer
pmm_fastq->reserve(part);
copy(_part + _size, _part + total_filled, part);
part_filled = total_filled - _size;
return true;
}
//----------------------------------------------------------------------------------
// Skip to next EOL from the current position in a buffer
bool CFastqReader::SkipNextEOL(uchar *part, int64 &pos, int64 max_pos)
{
int64 i;
for (i = pos; i < max_pos - 2; ++i)
if ((part[i] == '\n' || part[i] == '\r') && !(part[i + 1] == '\n' || part[i + 1] == '\r'))
break;
if (i >= max_pos - 2)
return false;
pos = i + 1;
return true;
}
void CFastqReader::GetFullLineFromEnd(int64& line_sart, int64& line_end, uchar* buff, int64& pos)
{
while (pos >= 0 && buff[pos] != '\n' && buff[pos] != '\r')
--pos;
line_end = pos + 1;
if (pos >= 0 && (buff[pos] == '\n' || buff[pos] == '\r'))
{
--pos;
if (pos >= 0 && buff[pos] != buff[pos + 1] && (buff[pos] == '\n' || buff[pos] == '\r'))
--pos;
}
while (pos >= 0 && buff[pos] != '\n' && buff[pos] != '\r')
--pos;
line_sart = pos + 1;
}
//************************************************************************************************************
// CFastqReaderDataSrc - data source for FastqReader
//************************************************************************************************************
void CFastqReaderDataSrc::init_stream()
{
switch (compression_type)
{
case CompressionType::plain:
in_data_pos = 0;
break;
case CompressionType::gzip:
stream.zalloc = Z_NULL;
stream.zfree = Z_NULL;
stream.opaque = Z_NULL;
stream.avail_in = 0;
stream.next_in = Z_NULL;
if (inflateInit2(&stream, 31) != Z_OK)
{
cerr << "Error while reading gz file\n";
exit(1);
}
stream.avail_in = (uint32)in_data_size;
stream.next_in = in_data;
break;
case CompressionType::bzip2:
_bz_stram.bzalloc = nullptr;
_bz_stram.bzfree = nullptr;
_bz_stram.opaque = nullptr;
_bz_stram.avail_in = 0;
_bz_stram.next_in = nullptr;
if (BZ2_bzDecompressInit(&_bz_stram, 0, 0) != BZ_OK)
{
cerr << "Error while reading bz2 file\n";
exit(1);
}
_bz_stram.avail_in = (uint32)in_data_size;
_bz_stram.next_in = (char*)in_data;
break;
default:
break;
}
}
//----------------------------------------------------------------------------------
void CFastqReaderDataSrc::SetQueue(CBinaryPackQueue* _binary_pack_queue, CMemoryPool *_pmm_binary_file_reader)
{
binary_pack_queue = _binary_pack_queue;
pmm_binary_file_reader = _pmm_binary_file_reader;
}
//----------------------------------------------------------------------------------
bool CFastqReaderDataSrc::Finished()
{
return end_reached;
}
//----------------------------------------------------------------------------------
uint64 CFastqReaderDataSrc::read(uchar* buff, uint64 size, bool& last_in_file)
{
last_in_file = false;
if (!in_progress)
{
if (!binary_pack_queue->pop(in_data, in_data_size, file_part, compression_type))
{
end_reached = true;
return 0;
}
in_progress = true;
init_stream();
}
//reading
if (compression_type == CompressionType::gzip)
{
stream.next_out = buff;
stream.avail_out = (uint32)size;
int ret;
do
{
if (!stream.avail_in)
{
pmm_binary_file_reader->free(in_data);
in_data = nullptr;
if (!binary_pack_queue->pop(in_data, in_data_size, file_part, compression_type))
return 0;
stream.avail_in = (uint32)in_data_size;
stream.next_in = in_data;
}
ret = inflate(&stream, Z_NO_FLUSH);
switch (ret)
{
case Z_NEED_DICT:
ret = Z_DATA_ERROR; /* and fall through */
case Z_DATA_ERROR:
cerr << "Some error while reading gzip file\n";
exit(1);
case Z_MEM_ERROR:
inflateEnd(&stream);
return ret;
}
if (ret == Z_STREAM_END)
{
uchar* tmp_data = nullptr;
uint64 tmp_size = 0;
bool multistream = stream.avail_in || binary_pack_queue->peek_next_pack(tmp_data, tmp_size);
bool garbage = false;
if (multistream)
{
if (stream.avail_in + tmp_size < 2)
{
cerr << "Some error while reading gzip file\n";
exit(1);
}
uchar b1, b2;
if (stream.avail_in >= 2)
{
b1 = stream.next_in[0];
b2 = stream.next_in[1];
}
else if (stream.avail_in == 1)
{
b1 = stream.next_in[0];
b2 = tmp_data[0];
}
else
{
b1 = tmp_data[0];
b2 = tmp_data[1];
}
garbage = b1 != 0x1f || b2 != 0x8b;
}
//bool multistream = stream.avail_in || !binary_pack_queue->is_next_last();
if (!multistream || garbage)
{
pmm_binary_file_reader->free(in_data);
in_data = nullptr;
inflateEnd(&stream);
in_progress = false;
//pull end
bool queue_end = !binary_pack_queue->pop(in_data, in_data_size, file_part, compression_type);
if (!queue_end && file_part != FilePart::End && !garbage)
{
cerr << "Error: An internal error occurred. Please contact authors\n";
}
if (garbage)
{
//ignore rest (garbage) data of a current file
FilePart tmpfilepart = file_part;
while (tmpfilepart != FilePart::End)
{
uchar *tmp;
uint64 tmpsize;
CompressionType tmpcomptype;
binary_pack_queue->pop(tmp, tmpsize, tmpfilepart, tmpcomptype);
}
}
last_in_file = true;
break;
}
else //multiple streams in one file
{
//equivalent of inflateReset
//inflateEnd(&stream);
//if (inflateInit2(&stream, 31) != Z_OK)
//{
// cerr << "Error while reading gzip file\n";
// exit(1);
//}
if (inflateReset(&stream) != Z_OK)
{
cerr << "Error while reading gzip file\n";
exit(1);
}
}
}
} while (stream.avail_out);
return size - stream.avail_out;
}
else if (compression_type == CompressionType::bzip2)
{
_bz_stram.next_out = (char*)buff;
_bz_stram.avail_out = (uint32)size;
int ret;
do
{
if (!_bz_stram.avail_in)
{
pmm_binary_file_reader->free(in_data);
in_data = nullptr;
binary_pack_queue->pop(in_data, in_data_size, file_part, compression_type);
_bz_stram.avail_in = (uint32)in_data_size;
_bz_stram.next_in = (char*)in_data;
}
ret = BZ2_bzDecompress(&_bz_stram);
if (ret == BZ_PARAM_ERROR || ret == BZ_DATA_ERROR || ret == BZ_DATA_ERROR_MAGIC || ret == BZ_MEM_ERROR)
{
BZ2_bzDecompressEnd(&_bz_stram);
cerr << "bz2 reading error\n";
}
if (ret == BZ_STREAM_END)
{
bool multistream = _bz_stram.avail_in || !binary_pack_queue->is_next_last();
if (!multistream)
{
pmm_binary_file_reader->free(in_data);
in_data = nullptr;
BZ2_bzDecompressEnd(&_bz_stram);
in_progress = false;
//pull end
bool queue_end = !binary_pack_queue->pop(in_data, in_data_size, file_part, compression_type);
if (!queue_end && file_part != FilePart::End)
{
cerr << "Error: An internal error occurred. Please contact authors\n";
}
last_in_file = true;
break;
}
else
{
BZ2_bzDecompressEnd(&_bz_stram);
if (BZ2_bzDecompressInit(&_bz_stram, 0, 0) != BZ_OK)
{
cerr << "Error while reading bz2 file\n";
exit(1);
}
}
}
} while (_bz_stram.avail_out);
return size - _bz_stram.avail_out;
}
else if (compression_type == CompressionType::plain)
{
uint64 out_pos = 0;
do
{
if (in_data_pos >= in_data_size)
{
pmm_binary_file_reader->free(in_data);
in_data = nullptr;
binary_pack_queue->pop(in_data, in_data_size, file_part, compression_type);
if (file_part == FilePart::End)
{
in_progress = false;
last_in_file = true;
break;
}
in_data_pos = 0;
}
uint64 in_left = in_data_size - in_data_pos;
uint64 out_left = size - out_pos;
uint64 n_copy = min(in_left, out_left);
memcpy(buff + out_pos, in_data + in_data_pos, n_copy);
in_data_pos += n_copy;
out_pos += n_copy;
} while (out_pos < size);
return out_pos;
}
else
{
cerr << "Error: unknown compression\n";
exit(1);
}
}
//************************************************************************************************************
// CWFastqReader - wrapper for multithreading purposes
//************************************************************************************************************
CWFastqReader::CWFastqReader(CKMCParams &Params, CKMCQueues &Queues, CBinaryPackQueue* _binary_pack_queue)
{
mm = Queues.mm;
pmm_fastq = Queues.pmm_fastq;
pmm_binary_file_reader = Queues.pmm_binary_file_reader;
//input_files_queue = Queues.input_files_queue;
binary_pack_queue = _binary_pack_queue;
missingEOL_at_EOF_counter = Queues.missingEOL_at_EOF_counter;
bam_task_manager = Queues.bam_task_manager;
part_size = Params.fastq_buffer_size;
part_queue = Queues.part_queue;
file_type = Params.file_type;
kmer_len = Params.p_k;
fqr = nullptr;
}
//----------------------------------------------------------------------------------
CWFastqReader::~CWFastqReader()
{
}
//----------------------------------------------------------------------------------
void CWFastqReader::operator()()
{
uchar *part;
uint64 part_filled;
fqr = new CFastqReader(mm, pmm_fastq, file_type, kmer_len, binary_pack_queue, pmm_binary_file_reader, bam_task_manager, part_queue, nullptr, missingEOL_at_EOF_counter);
fqr->SetPartSize(part_size);
if (file_type == bam)
{
fqr->ProcessBam();
}
else
{
fqr->Init();
ReadType read_type;
while (fqr->GetPartNew(part, part_filled, read_type))
part_queue->push(part, part_filled, read_type);
}
delete fqr;
part_queue->mark_completed();
}
//************************************************************************************************************
// CWStatsFastqReader - wrapper for multithreading purposes
//************************************************************************************************************
CWStatsFastqReader::CWStatsFastqReader(CKMCParams &Params, CKMCQueues &Queues, CBinaryPackQueue* _binary_pack_queue)
{
mm = Queues.mm;
pmm_fastq = Queues.pmm_fastq;
pmm_binary_file_reader = Queues.pmm_binary_file_reader;
binary_pack_queue = _binary_pack_queue;
bam_task_manager = Queues.bam_task_manager;
part_size = Params.fastq_buffer_size;
stats_part_queue = Queues.stats_part_queue;
file_type = Params.file_type;
kmer_len = Params.p_k;
missingEOL_at_EOF_counter = Queues.missingEOL_at_EOF_counter;
fqr = nullptr;
}
//----------------------------------------------------------------------------------
CWStatsFastqReader::~CWStatsFastqReader()
{
}
//----------------------------------------------------------------------------------
void CWStatsFastqReader::operator()()
{
uchar *part;
uint64 part_filled;
fqr = new CFastqReader(mm, pmm_fastq, file_type, kmer_len, binary_pack_queue, pmm_binary_file_reader, bam_task_manager, nullptr, stats_part_queue, missingEOL_at_EOF_counter);
fqr->SetPartSize(part_size);
if (file_type == bam)
{
fqr->ProcessBam();
}
else
{
fqr->Init();
bool finished = false;
ReadType read_type;
while (fqr->GetPartNew(part, part_filled, read_type) && !finished)
{
if (!stats_part_queue->push(part, part_filled, read_type))
{
finished = true;
pmm_fastq->free(part);
binary_pack_queue->ignore_rest();
fqr->IgnoreRest();
break;
}
}
}
delete fqr;
stats_part_queue->mark_completed();
}
// ***** EOF
|