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/*******************************************************************************
* $Id: FileSML.cpp,v 1.22 2004/04/26 21:13:58 darling Exp $
* This file is copyright 2002-2007 Aaron Darling and authors listed in the AUTHORS file.
* Please see the file called COPYING for licensing, copying, and modification
* Please see the file called COPYING for licensing details.
* **************
******************************************************************************/
#include "libMems/FileSML.h"
// for CreateTempFileName():
#include "libMems/Aligner.h"
#include "libGenome/gnFilter.h"
#include "libGenome/gnRAWSource.h"
#include <algorithm>
#include <cmath>
#include "boost/filesystem/operations.hpp"
using namespace std;
using namespace genome;
namespace mems {
FileSML& FileSML::operator=(const FileSML& sa)
{
SortedMerList::operator=( sa );
filename = sa.filename;
sarray_start_offset = sa.sarray_start_offset;
seq_coords = sa.seq_coords;
sarfile.open(filename.c_str(), ios::binary | ios::in );
if(!sarfile.is_open()){
DebugMsg("FileSML::=: Unable to open suffix array file.\n");
sarfile.clear();
return *this;
}
return *this;
}
void FileSML::Clear() {
SortedMerList::Clear();
filename = "";
sarfile.close();
sarray_start_offset = 0;
seq_coords.clear();
}
void FileSML::LoadFile(const string& fname){
filename = fname;
sarfile.open(fname.c_str(), ios::binary | ios::in );
if(!sarfile.is_open()){
sarfile.clear();
Throw_gnExMsg( FileNotOpened(), "Unable to open file.\n");
}
// read the header into a temporary header struct just
// in case it's bogus
SMLHeader tmp_header;
sarfile.read((char*)&tmp_header, sizeof(struct SMLHeader));
if(sarfile.gcount() < (int)sizeof(struct SMLHeader)){
sarfile.clear();
Throw_gnExMsg( FileUnreadable(), "Unable to read file.");
}
if(tmp_header.version != FormatVersion()){
Throw_gnExMsg( FileUnreadable(), "Unsupported file format.");
}
header = tmp_header;
SetMerMaskSize( header.seed_weight );
seed_mask = mer_mask;
SetMerMaskSize( header.seed_length );
//header is ok. read the sequence.
gnSeqI seq_len = header.length;
if(header.circular)
seq_len += header.seed_length - 1;
binary_seq_len = ((uint64)seq_len * (uint64)header.alphabet_bits) / 32;
if(((uint64)seq_len * (uint64)header.alphabet_bits) % 32 != 0)
binary_seq_len++;
binary_seq_len+=2; //fix for access violations.
if(sequence != NULL)
delete[] sequence;
sequence = new uint32[binary_seq_len];
sarfile.read((char*)sequence, binary_seq_len*sizeof(uint32));
if(sarfile.gcount() < (int64)(binary_seq_len*sizeof(uint32))){
sarfile.clear();
Throw_gnExMsg( FileUnreadable(), "Error reading sequence data.");
}
sarray_start_offset = sarfile.tellg();
sarfile.seekg(sarray_start_offset + sizeof(gnSeqI) * header.length);
if(!sarfile.good()){
sarfile.clear();
Throw_gnExMsg( FileUnreadable(), "Premature end of file.");
}
filename = fname;
// create a memory-map to the data of interest
sardata.open(fname);
// check whether there is a .coords mask file to read
string coordfile = filename + ".coords";
ifstream coord_in( coordfile.c_str() );
if( coord_in.is_open() ){
seq_coords.clear();
int64 cur_coord;
while( coord_in >> cur_coord ){
seq_coords.push_back( cur_coord );
}
}
}
void FileSML::OpenForWriting( boolean truncate ){
// Open smlfile for writing
boolean was_open = sarfile.is_open();
if(was_open)
sarfile.close();
if( truncate )
sarfile.open(filename.c_str(), ios::binary | ios::in | ios::out | ios::trunc );
else
sarfile.open(filename.c_str(), ios::binary | ios::in | ios::out );
if(!sarfile.is_open() || !sarfile.good()){
sarfile.clear();
if(was_open)
sarfile.open(filename.c_str(), ios::binary | ios::in );
Throw_gnExMsg(FileNotOpened(), "Unable to open file for writing.");
}
}
boolean FileSML::WriteHeader(){
if(!sarfile.is_open()){
Throw_gnExMsg(IOStreamFailed(), "File is not valid.");
}
boolean success = true;
const char* errormsg = "";
// Open sarfile for writing and write new header.
OpenForWriting( false );
sarfile.write((char*)&header, sizeof(struct SMLHeader));
if(!sarfile.good()){
errormsg = "Error writing header to disk.";
success = false;
}
// reopen the sorted mer list file read-only
sarfile.close();
sarfile.open(filename.c_str(), ios::binary | ios::in );
if(!sarfile.is_open()){
errormsg = "Error opening sorted mer list file.";
success = false;
}
if(!success)
Throw_gnExMsg(IOStreamFailed(), errormsg);
return success;
}
gnSeqI FileSML::UniqueMerCount(){
gnSeqI tmp_count = header.unique_mers;
SortedMerList::UniqueMerCount();
if(tmp_count != header.unique_mers)
WriteHeader();
return header.unique_mers;
}
//change the description in memory and on disk
void FileSML::SetDescription(const string& d){
strncpy(header.description, d.c_str(), DESCRIPTION_SIZE-1);
WriteHeader();
}
void FileSML::SetID(const sarID_t d){
header.id = d;
WriteHeader();
}
extern "C" {
#include "libMems/dmSML/dmsort.h"
}
char** FileSML::tmp_paths = NULL;
void FileSML::registerTempPath( const string& path ) {
string tmp_path = path;
// add trailing path separator if necessary
#ifdef WIN32
if( tmp_path[ tmp_path.size() - 1 ] != '\\' )
tmp_path += "\\";
#else
if( tmp_path[ tmp_path.size() - 1 ] != '/' )
tmp_path += "/";
#endif
if( tmp_paths == NULL ){
tmp_paths = new char*[1];
tmp_paths[ 0 ] = NULL;
}
int path_count = 0;
while( tmp_paths[ path_count ] != NULL )
path_count++;
// create a new array with room for another element
char** tmp_tmp_paths = new char*[ path_count+1 ];
// copy old elements
for( int pathI = 0; pathI < path_count; pathI++ )
tmp_tmp_paths[ pathI ] = tmp_paths[ pathI ];
// add new element
tmp_tmp_paths[ path_count ] = new char[ tmp_path.size() + 1 ];
strncpy( tmp_tmp_paths[ path_count ], tmp_path.c_str(), tmp_path.size() + 1 );
// set null terminator element
tmp_tmp_paths[ path_count + 1 ] = NULL;
// set new paths
char** old_paths = tmp_paths;
tmp_paths = tmp_tmp_paths;
// free old array
delete[] old_paths;
}
const char* FileSML::getTempPath( int pathI ){
return tmp_paths[ pathI ];
}
int FileSML::getTempPathCount(){
int path_count = 0;
while( tmp_paths && tmp_paths[ path_count ] != NULL )
path_count++;
return path_count;
}
void maskNNNNN( const gnSequence& in_seq, gnSequence& out_seq, vector< int64 >& seq_coords, int mask_n_length ) {
gnSeqI seqI = 1;
gnSeqI read_length = 1024*1024;
string cur_seq;
gnSeqI n_count = 0;
gnSeqI n_stretch_start = 0;
gnSeqI n_stretch_end = 1;
while( seqI <= in_seq.length() ){
read_length = seqI + read_length < in_seq.length() ? read_length : in_seq.length() - seqI + 1;
in_seq.ToString( cur_seq, read_length, seqI );
uint charI = 0;
for( ; charI < cur_seq.size(); charI++ ){
if( cur_seq[ charI ] == 'N' || cur_seq[ charI ] == 'n' ){
if( n_count == 0 ){
n_stretch_start = seqI + charI;
}
n_count++;
}else{
if( n_count > mask_n_length ){
if( n_stretch_start - n_stretch_end != 0 ){
// Add the sequence region to the output sequence
out_seq += in_seq.subseq( n_stretch_end, n_stretch_start - n_stretch_end );
// add the masked coordinates
seq_coords.push_back( n_stretch_end );
seq_coords.push_back( n_stretch_start - 1 );
}
// update n_stretch_end to the first non N character
n_stretch_end = seqI + charI;
}
n_count = 0;
}
}
seqI += read_length;
}
out_seq += in_seq.subseq( n_stretch_end, seqI - n_stretch_end );
// add the masked coordinates
seq_coords.push_back( n_stretch_end );
seq_coords.push_back( seqI - 1 );
}
// use dmSML to construct the SML
// then read it in using LoadFile()
void FileSML::dmCreate(const gnSequence& seq, const uint64 seed){
// Filter NNNNNs
gnSequence masked_seq;
seq_coords.clear();
maskNNNNN( seq, masked_seq, seq_coords, 0 );
// write a raw sequence to a tmp file stored in the first scratch path
string rawfile = CreateTempFileName("dm_rawseq");
gnRAWSource::Write( masked_seq, rawfile.c_str() );
// write a sequence coordinate file
if( seq_coords.size() > 0 ){
string coordfile = filename + ".coords";
ofstream coord_out( coordfile.c_str() );
if( !coord_out.is_open() ){
cerr << "Could not open " << coordfile << endl;
throw "";
}
for( int coordI = 0; coordI < seq_coords.size(); coordI+=2 ){
coord_out << seq_coords[ coordI ] << '\t' << seq_coords[ coordI + 1 ] << endl;
}
coord_out.close();
}
// run dmSML
const char* const* scratch_paths = (const char* const*)tmp_paths;
sarfile.close();
int rval = dmSML( rawfile.c_str(), filename.c_str(), scratch_paths, seed );
if( rval != 0 )
cerr << "Crap. It's broke, return value " << rval << endl;
boost::filesystem::remove( rawfile );
// load the sorted mer list
LoadFile( filename );
}
void FileSML::Create(const gnSequence& seq, const uint64 seed){
vector<bmer> sml_array;
bool is_spaced_seed = getSeedWeight(seed) != getSeedLength(seed);
OpenForWriting( true );
try{
SortedMerList::Create( seq, seed );
if( is_spaced_seed )
FillDnaSeedSML(seq, sml_array);
else
FillSML(seq, sml_array);
}catch(...){
// if there was a memory allocation error then
// try using dmSML to do an external sort
sarfile.clear();
sarfile.close();
sarfile.clear();
if( sequence != NULL )
delete[] sequence;
binary_seq_len = 0;
dmCreate( seq, seed );
}
// RadixSort(s_array);
sort(sml_array.begin(), sml_array.end(), &bmer_lessthan);
/* now write out the file header */
sarfile.write((char*)&header, sizeof(struct SMLHeader));
if(!sarfile.good()){
sarfile.clear();
Throw_gnExMsg( IOStreamFailed(), "Error writing sorted mer list header to disk.\n");
}
/* write out the actual sequence */
sarfile.write((char*)sequence, binary_seq_len*sizeof(uint32));
sarray_start_offset = sarfile.tellg();
/* write out the sorted mer list */
for(gnSeqI suffixI=0; suffixI < sml_array.size(); suffixI++)
sarfile.write((char*)&(sml_array[suffixI].position), sizeof(smlSeqI_t));
sarfile.flush();
if(!sarfile.good()){
sarfile.clear();
Throw_gnExMsg( IOStreamFailed(), "Error writing sorted mer list to disk.\n");
}
// reopen the sorted mer list file read-only
sarfile.close();
sarfile.open(filename.c_str(), ios::binary | ios::in );
if(!sarfile.is_open())
Throw_gnExMsg( FileNotOpened(), "FileSML::Create: Error opening sorted mer list file.\n");
sardata.open(filename);
}
bmer FileSML::operator[](gnSeqI index)
{
bmer tmp_mer;
tmp_mer.position = base()[index];
tmp_mer.mer = GetSeedMer(tmp_mer.position);
return tmp_mer;
}
boolean FileSML::Read(vector<bmer>& readVector, gnSeqI size, const gnSeqI offset)
{
if(!sarfile.is_open()){
DebugMsg("FileSML::Read: Error sar file not open.\n");
return false;
}
gnSeqI total_len = SMLLength();
if(offset >= total_len){
readVector.clear();
return false;
}
gnSeqI readlen = offset + size < total_len ? size : total_len - offset;
readVector.resize( readlen );
//copy data to the vector
for(gnSeqI j=0; j < readlen; j++){
bmer tmp_mer;
tmp_mer.position = base()[offset+j];
if( tmp_mer.position > header.length ){
string errmsg = "Corrupted SML, position ";
errmsg += tmp_mer.position + " is out of range\n";
ErrorMsg( errmsg );
cerr << errmsg;
}else
tmp_mer.mer = GetSeedMer(tmp_mer.position);
readVector[ j ] = tmp_mer;
}
return true;
}
void FileSML::BigCreate(const gnSequence& seq, const uint32 split_levels, const uint32 mersize){
// unsigned long freemem = wxGetFreeMemory(); //get the amount of free memory.
// unsigned long neededmem = GetNeededMemory(seq.length());
// if(neededmem >= freemem && neededmem > MEMORY_MINIMUM){ // divide and conquer
if(split_levels > 0){ // split_levels defines the number of times to divide and conquer
uint64 midpoint = seq.length() / 2;
midpoint = (midpoint * header.alphabet_bits) / 32;
midpoint = (midpoint / header.alphabet_bits) * 32;
gnSequence seqA = seq.subseq(1, midpoint);
gnSequence seqB = seq.subseq(1 + midpoint, seq.length() - midpoint);
seqA.setCircular(false);
seqB.setCircular(false);
cout << "Splitting " << seq.length() << " to " << seqA.length() << " and " << seqB.length() << "\n";
//create the first sar
string temp_sarfile = CreateTempFileName("bdsa_split");
FileSML* temp_sar = this->Clone();
temp_sar->filename = temp_sarfile.c_str();
temp_sar->BigCreate(seqA, split_levels - 1, mersize);
//create the second sar
string temp_sarfile2 = CreateTempFileName("bdsa_split");
FileSML* temp_sar2 = this->Clone();
temp_sar2->filename = temp_sarfile2.c_str();
temp_sar2->BigCreate(seqB, split_levels - 1, mersize);
//merge them to this file
cout << "Merging " << seqA.length() << " and " << seqB.length() << "\n";
Merge(*temp_sar, *temp_sar2);
//free up RAM
delete temp_sar;
delete temp_sar2;
//erase the temp files.
boost::filesystem::remove(temp_sarfile);
boost::filesystem::remove(temp_sarfile2);
}else{
Create(seq, mersize);
}
}
void FileSML::RadixSort(vector<bmer>& s_array){
vector<bmer> *source_buckets;
vector<bmer> *tmp_buckets;
vector<bmer> *buckets;
uint32 radix_size = 11;
uint64 radix_mask = 0xFFFFFFFF;
radix_mask <<= 32;
radix_mask |= 0xFFFFFFFF;
radix_mask >>= 64 - radix_size;
uint32 bucket_count = (uint32) pow((double)2, (double)radix_size);
uint32 cur_shift_bits = 0;
buckets = new vector<bmer>[bucket_count];
source_buckets = new vector<bmer>[bucket_count];
uint64 cur_bucket;
for(uint32 merI = 0; merI < s_array.size(); merI++){
cur_bucket = s_array[merI].mer & radix_mask;
source_buckets[cur_bucket].push_back(s_array[merI]);
}
s_array.clear();
cur_shift_bits += radix_size;
radix_mask <<= radix_size;
while(cur_shift_bits < 64){
for(uint32 bucketI = 0; bucketI < bucket_count; bucketI++){
for(uint32 merI = 0; merI < source_buckets[bucketI].size(); merI++){
cur_bucket = source_buckets[bucketI][merI].mer & radix_mask;
cur_bucket >>= cur_shift_bits;
buckets[cur_bucket].push_back(source_buckets[bucketI][merI]);
}
source_buckets[bucketI].clear();
}
cur_shift_bits += radix_size;
radix_mask <<= radix_size;
tmp_buckets = source_buckets;
source_buckets = buckets;
buckets = tmp_buckets;
}
s_array.clear();
for(uint32 bucketI = 0; bucketI < bucket_count; bucketI++){
for(uint32 merI = 0; merI < source_buckets[bucketI].size(); merI++){
s_array.push_back(source_buckets[bucketI][merI]);
}
source_buckets[bucketI].clear();
}
delete[] source_buckets;
delete[] buckets;
}
//Merges the supplied sorted mer lists into this one, overwriting the existing sml.
//KNOWN BUG: The first sorted mer list must have (length * alphabet_bits) / word_bits == 0
//for Merge to work properly.
void FileSML::Merge(SortedMerList& sa, SortedMerList& sa2){
STACK_TRACE_START
SMLHeader sa_head = sa.GetHeader();
SMLHeader sa_head2 = sa2.GetHeader();
//basic copying
header = sa_head;
//take the smaller mer_size
if(sa_head.seed_length < sa_head2.seed_length){
header.seed_length = sa_head.seed_length;
mer_mask = sa.GetMerMask();
}else{
header.seed_length = sa_head2.seed_length;
mer_mask = sa2.GetMerMask();
}
header.unique_mers = NO_UNIQUE_COUNT;
header.length += sa_head2.length;
//allocate some memory
const uint32 SEQ_BUFFER_SIZE = 200000;
Array<uint32> seq_buf ( SEQ_BUFFER_SIZE + header.seed_length );
//do some sanity checks on the sars we're merging.
if(sa_head.alphabet_bits != sa_head2.alphabet_bits ||
sa_head.version != sa_head2.version ||
memcmp(sa_head.translation_table, sa_head2.translation_table, UINT8_MAX)){
Throw_gnExMsg(SMLMergeError(), "Incompatible sorted mer lists.");
}
OpenForWriting( true );
//write the header
sarfile.write((char*)&header, sizeof(struct SMLHeader));
if(!sarfile.good()){
sarfile.clear();
sarfile.close();
sarfile.open(filename.c_str(), ios::binary | ios::in );
Throw_gnExMsg(IOStreamFailed(), "Error writing sorted mer list header to disk.");
}
//copy sequence data into memory.
uint32 binary_seq_len = (header.length * header.alphabet_bits) / 32;
if((header.length * header.alphabet_bits) % 32 > 0)
binary_seq_len++;
//The +1 is to avoid access violations when copying in the
//binary sequence before shifting.
if( sequence != NULL )
delete[] sequence;
sequence = new uint32[binary_seq_len+1];
sa.GetBSequence(sequence, sa_head.length, 0);
uint32 bseq_len1 = (sa_head.length * sa_head.alphabet_bits) / 32;
uint32 bseq_remainder = (sa_head.length * sa_head.alphabet_bits) % 32;
if(bseq_remainder > 0){
sa2.GetBSequence(&(sequence[bseq_len1]), sa_head2.length, 0);
//mask off the end of the first sequence
uint32 end_mask = 0xFFFFFFFF;
end_mask <<= bseq_remainder;
sequence[bseq_len1] &= end_mask;
//shift the second sequence over.
for(uint32 i=bseq_len1; i < binary_seq_len; i++){
uint32 tmp = sequence[i+1];
tmp >>= 32 - bseq_remainder;
sequence[i] |= tmp;
sequence[i+1] <<= bseq_remainder;
}
}else
sa2.GetBSequence(&(sequence[bseq_len1]), sa_head2.length, 0);
//write the sequence
sarfile.write((char*)sequence, binary_seq_len * sizeof(uint32));
sarray_start_offset = sarfile.tellg();
//get new mers in the middle
vector<bmer> middle_mers;
bmer mid_mer;
for(uint32 midI = sa_head.length - header.seed_length + 1; midI < sa_head.length; midI++){
mid_mer.position = midI;
mid_mer.mer = GetMer(midI);
middle_mers.push_back(mid_mer);
}
sort(middle_mers.begin(), middle_mers.end(), &bmer_lessthan);
//put a special mer at the end which will never go into the sorted mer list
//since every possible mer is less than it.
mid_mer.mer = 0xFFFFFFFF;
mid_mer.mer <<= 32;
mid_mer.mer |= 0xFFFFFFFF;
mid_mer.position = GNSEQI_END;
middle_mers.push_back(mid_mer);
//merge and write the sorted mer lists
vector<bmer> array1, array2;
uint32 SAR_BUFFER_SIZE = SEQ_BUFFER_SIZE/2; //actual size is this number * 13 bytes
uint32 k=0, l=0, midI=0;
uint32 m = 0, n = 0;
gnSeqI bufferI=0;
do{
//mergesort them
while(m < array1.size() && n < array2.size()){
if(array1[m].mer <= array2[n].mer){
if(array1[m].mer <= middle_mers[midI].mer){
seq_buf.data[bufferI] = array1[m].position;
m++;
bufferI++;
}else{
seq_buf.data[bufferI] = middle_mers[midI].position;
midI++;
bufferI++;
}
}else if(array2[n].mer <= middle_mers[midI].mer){
seq_buf.data[bufferI] = array2[n].position + sa_head.length;
n++;
bufferI++;
}else{
seq_buf.data[bufferI] = middle_mers[midI].position;
midI++;
bufferI++;
}
if(bufferI == SEQ_BUFFER_SIZE){
sarfile.write((char*)seq_buf.data, bufferI * sizeof(uint32));
bufferI = 0;
}
}
if(m == array1.size()){
sa.Read(array1, SAR_BUFFER_SIZE, k);
k += array1.size();
m = 0;
}
if(n == array2.size()){
sa2.Read(array2, SAR_BUFFER_SIZE, l);
l += array2.size();
n = 0;
}
}while(array1.size() != 0 && array2.size() != 0);
if(bufferI > 0)
sarfile.write((char*)seq_buf.data, (bufferI)*sizeof(uint32));
//consolidate the remaining mers to a known vector
vector<bmer> remaining_mers;
for(;m < array1.size(); m++)
remaining_mers.push_back(array1[m]);
for(;n < array2.size(); n++){
remaining_mers.push_back(array2[n]);
remaining_mers[remaining_mers.size()-1].position += sa_head.length;
}
for(;midI < middle_mers.size() - 1; midI++)
remaining_mers.push_back(middle_mers[midI]);
//merge them with the remaining middle_mers
sort(remaining_mers.begin(), remaining_mers.end(), &bmer_lessthan);
uint32 remI = 0;
for(;remI < remaining_mers.size(); remI++)
seq_buf.data[remI] = remaining_mers[remI].position;
if(remI > 0)
sarfile.write((char*)seq_buf.data, (remI)*sizeof(uint32));
if(!sarfile.good()){
sarfile.clear();
sarfile.close();
sarfile.open(filename.c_str(), ios::binary | ios::in );
Throw_gnExMsg(IOStreamFailed(), "Error writing position array.");
}
// reopen the sorted mer list file read-only
sarfile.close();
sarfile.open(filename.c_str(), ios::binary | ios::in );
if(!sarfile.is_open()){
sarfile.clear();
Throw_gnExMsg(FileNotOpened(), "Error opening sorted mer list file.");
}
STACK_TRACE_END
}
}
|