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/*
* ryanscommand.cpp
* Mothur
*
* Created by westcott on 9/23/10.
* Copyright 2010 Schloss Lab. All rights reserved.
*
*/
#include "clusterfragmentscommand.h"
#include "needlemanoverlap.hpp"
//**********************************************************************************************************************
//sort by unaligned
inline bool comparePriority(seqRNode first, seqRNode second) {
bool better = false;
if (first.length > second.length) {
better = true;
}else if (first.length == second.length) {
if (first.numIdentical > second.numIdentical) {
better = true;
}
}
return better;
}
//**********************************************************************************************************************
vector<string> ClusterFragmentsCommand::setParameters(){
try {
CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","fasta-name",false,true,true); parameters.push_back(pfasta);
CommandParameter pname("name", "InputTypes", "", "", "namecount", "none", "none","name",false,false,true); parameters.push_back(pname);
CommandParameter pcount("count", "InputTypes", "", "", "namecount", "none", "none","count",false,false,true); parameters.push_back(pcount);
CommandParameter pdiffs("diffs", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pdiffs);
CommandParameter ppercent("percent", "Number", "", "0", "", "", "","",false,false); parameters.push_back(ppercent);
CommandParameter pseed("seed", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pseed);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
abort = false; calledHelp = false;
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
outputTypes["name"] = tempOutNames;
outputTypes["count"] = tempOutNames;
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string ClusterFragmentsCommand::getHelpString(){
try {
string helpString = "";
helpString += "The cluster.fragments command groups sequences that are part of a larger sequence.\n";
helpString += "The cluster.fragments command outputs a new fasta and name or count file.\n";
helpString += "The cluster.fragments command parameters are fasta, name, count, diffs and percent. The fasta parameter is required, unless you have a valid current file. \n";
helpString += "The names parameter allows you to give a list of seqs that are identical. This file is 2 columns, first column is name or representative sequence, second column is a list of its identical sequences separated by commas.\n";
helpString += "The diffs parameter allows you to set the number of differences allowed, default=0. \n";
helpString += "The percent parameter allows you to set percentage of differences allowed, default=0. percent=2 means if the number of difference is less than or equal to two percent of the length of the fragment, then cluster.\n";
helpString += "You may use diffs and percent at the same time to say something like: If the number or differences is greater than 1 or more than 2% of the fragment length, don't merge. \n";
helpString += "The cluster.fragments command should be in the following format: \n";
helpString += "cluster.fragments(fasta=yourFastaFile, names=yourNamesFile) \n";
helpString += "Example cluster.fragments(fasta=amazon.fasta).\n";
;
return helpString;
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string ClusterFragmentsCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "fasta") { pattern = "[filename],fragclust.fasta"; }
else if (type == "name") { pattern = "[filename],fragclust.names"; }
else if (type == "count") { pattern = "[filename],fragclust.count_table"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->setControl_pressed(true); }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
ClusterFragmentsCommand::ClusterFragmentsCommand(string option) : Command() {
try {
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else if(option == "category") { abort = true; calledHelp = true; }
else {
OptionParser parser(option, setParameters());
map<string, string> parameters = parser.getParameters();
ValidParameters validParameter;
fastafile = validParameter.validFile(parameters, "fasta");
if (fastafile == "not found") {
fastafile = current->getFastaFile();
if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter.\n"); }
else { m->mothurOut("You have no current fastafile and the fasta parameter is required.\n"); abort = true; }
}
else if (fastafile == "not open") { fastafile = ""; abort = true; }
else { current->setFastaFile(fastafile); }
if (outputdir == ""){ outputdir = util.hasPath(fastafile); }
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
namefile = validParameter.validFile(parameters, "name");
if (namefile == "not found") { namefile = ""; }
else if (namefile == "not open") { namefile = ""; abort = true; }
else { util.readNames(namefile, names, sizes); current->setNameFile(namefile); }
countfile = validParameter.validFile(parameters, "count");
if (countfile == "not open") { abort = true; countfile = ""; }
else if (countfile == "not found") { countfile = ""; }
else { ct.readTable(countfile, true, false); current->setCountFile(countfile); }
if ((countfile != "") && (namefile != "")) { m->mothurOut("When executing a cluster.fragments command you must enter ONLY ONE of the following: count or name.\n"); abort = true; }
string temp;
temp = validParameter.valid(parameters, "diffs"); if (temp == "not found"){ temp = "0"; }
util.mothurConvert(temp, diffs);
temp = validParameter.valid(parameters, "percent"); if (temp == "not found"){ temp = "0"; }
util.mothurConvert(temp, percent);
}
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "ClusterFragmentsCommand");
exit(1);
}
}
//**********************************************************************************************************************
int ClusterFragmentsCommand::execute(){
try {
if (abort) { if (calledHelp) { return 0; } return 2; }
long start = time(nullptr);
//reads fasta file and return number of seqs
int numSeqs = readFASTA(); //fills alignSeqs and makes all seqs active
if (m->getControl_pressed()) { return 0; }
if (numSeqs == 0) { m->mothurOut("Error reading fasta file...please correct.\n"); return 0; }
//sort seqs by length of unaligned sequence
sort(alignSeqs.begin(), alignSeqs.end(), comparePriority);
int count = 0;
//think about running through twice...
for (int i = 0; i < numSeqs; i++) {
if (alignSeqs[i].active) { //this sequence has not been merged yet
string iBases = alignSeqs[i].seq.getUnaligned();
//try to merge it with all smaller seqs
for (int j = i+1; j < numSeqs; j++) {
if (m->getControl_pressed()) { return 0; }
if (alignSeqs[j].active) { //this sequence has not been merged yet
string jBases = alignSeqs[j].seq.getUnaligned();
if (isFragment(iBases, jBases)) {
if (countfile != "") {
ct.mergeCounts(alignSeqs[i].names, alignSeqs[j].names);
}else {
//merge
alignSeqs[i].names += ',' + alignSeqs[j].names;
alignSeqs[i].numIdentical += alignSeqs[j].numIdentical;
}
alignSeqs[j].active = 0;
alignSeqs[j].numIdentical = 0;
count++;
}
}//end if j active
}//end if i != j
//remove from active list
alignSeqs[i].active = 0;
}//end if active i
if(i % 100 == 0) { m->mothurOutJustToScreen(toString(i) + "\t" + toString(numSeqs - count) + "\t" + toString(count)+"\n"); }
}
if(numSeqs % 100 != 0) { m->mothurOutJustToScreen(toString(numSeqs) + "\t" + toString(numSeqs - count) + "\t" + toString(count)+"\n"); }
string fileroot = outputdir + util.getRootName(util.getSimpleName(fastafile));
map<string, string> variables;
variables["[filename]"] = fileroot;
string newFastaFile = getOutputFileName("fasta", variables);
string newNamesFile = getOutputFileName("name", variables);
if (countfile != "") { newNamesFile = getOutputFileName("count", variables); }
if (m->getControl_pressed()) { return 0; }
m->mothurOut("\nTotal number of sequences before cluster.fragments was " + toString(alignSeqs.size()) + ".\n");
m->mothurOut("cluster.fragments removed " + toString(count) + " sequences.\n\n");
printData(newFastaFile, newNamesFile);
m->mothurOut("It took " + toString(time(nullptr) - start) + " secs to cluster " + toString(numSeqs) + " sequences.\n");
if (m->getControl_pressed()) { util.mothurRemove(newFastaFile); util.mothurRemove(newNamesFile); return 0; }
m->mothurOut("\nOutput File Names: \n");
m->mothurOut(newFastaFile); m->mothurOutEndLine();
m->mothurOut(newNamesFile); m->mothurOutEndLine();
outputNames.push_back(newFastaFile); outputNames.push_back(newNamesFile); outputTypes["fasta"].push_back(newFastaFile); outputTypes["name"].push_back(newNamesFile);
m->mothurOutEndLine();
//set fasta file as new current fastafile
string currentName = "";
itTypes = outputTypes.find("fasta");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setFastaFile(currentName); }
}
itTypes = outputTypes.find("name");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setNameFile(currentName); }
}
itTypes = outputTypes.find("count");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setCountFile(currentName); }
}
return 0;
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "execute");
exit(1);
}
}
//***************************************************************************************************************
bool ClusterFragmentsCommand::isFragment(string seq1, string seq2){
try {
bool fragment = false;
//exact match
int pos = seq1.find(seq2);
if (pos != string::npos) { return true; }
//no match, no diffs wanted
else if ((diffs == 0) && (percent == 0)) { return false; }
else { //try aligning and see if you can find it
//find number of acceptable differences for this sequence fragment
int totalDiffs = 0;
if (diffs == 0) { //you didnt set diffs you want a percentage
totalDiffs = floor((seq2.length() * (percent / 100.0)));
}else if (percent == 0) { //you didn't set percent you want diffs
totalDiffs = diffs;
}else if ((percent != 0) && (diffs != 0)) { //you want both, set total diffs to smaller of 2
totalDiffs = diffs;
int percentDiff = floor((seq2.length() * (percent / 100.0)));
if (percentDiff < totalDiffs) { totalDiffs = percentDiff; }
}
Alignment* alignment = new NeedlemanOverlap(-1.0, 1.0, -1.0, (seq1.length()+totalDiffs+1));
//use needleman to align
alignment->align(seq2, seq1);
string tempSeq2 = alignment->getSeqAAln();
string temp = alignment->getSeqBAln();
delete alignment;
//chop gap ends
int startPos = 0;
int endPos = tempSeq2.length()-1;
for (int i = 0; i < tempSeq2.length(); i++) { if (isalpha(tempSeq2[i])) { startPos = i; break; } }
for (int i = tempSeq2.length()-1; i >= 0; i--) { if (isalpha(tempSeq2[i])) { endPos = i; break; } }
//count number of diffs
int numDiffs = 0;
for (int i = startPos; i <= endPos; i++) {
if (tempSeq2[i] != temp[i]) { numDiffs++; }
}
if (numDiffs <= totalDiffs) { fragment = true; }
}
return fragment;
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "isFragment");
exit(1);
}
}
/**************************************************************************************************/
int ClusterFragmentsCommand::readFASTA(){
try {
ifstream inFasta;
util.openInputFile(fastafile, inFasta);
while (!inFasta.eof()) {
if (m->getControl_pressed()) { inFasta.close(); return 0; }
Sequence seq(inFasta); gobble(inFasta);
if (seq.getName() != "") { //can get "" if commented line is at end of fasta file
if (namefile != "") {
itSize = sizes.find(seq.getName());
if (itSize == sizes.end()) { m->mothurOut(seq.getName() + " is not in your names file, please correct.\n"); exit(1); }
else{
seqRNode tempNode(itSize->second, seq, names[seq.getName()], seq.getUnaligned().length());
alignSeqs.push_back(tempNode);
}
}else if(countfile != "") {
seqRNode tempNode(ct.getNumSeqs(seq.getName()), seq, seq.getName(), seq.getUnaligned().length());
alignSeqs.push_back(tempNode);
}else { //no names file, you are identical to yourself
seqRNode tempNode(1, seq, seq.getName(), seq.getUnaligned().length());
alignSeqs.push_back(tempNode);
}
}
}
inFasta.close();
return alignSeqs.size();
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "readFASTA");
exit(1);
}
}
/**************************************************************************************************/
void ClusterFragmentsCommand::printData(string newfasta, string newname){
try {
ofstream outFasta;
ofstream outNames;
util.openOutputFile(newfasta, outFasta);
if (countfile == "") { util.openOutputFile(newname, outNames); }
for (int i = 0; i < alignSeqs.size(); i++) {
if (alignSeqs[i].numIdentical != 0) {
alignSeqs[i].seq.printSequence(outFasta);
if (countfile == "") { outNames << alignSeqs[i].seq.getName() << '\t' << alignSeqs[i].names << endl; }
}
}
outFasta.close();
if (countfile == "") { outNames.close(); }
else { ct.printTable(newname); }
}
catch(exception& e) {
m->errorOut(e, "ClusterFragmentsCommand", "printData");
exit(1);
}
}
/**************************************************************************************************/
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