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/*
* mergegroupscommand.cpp
* mothur
*
* Created by westcott on 1/24/11.
* Copyright 2011 Schloss Lab. All rights reserved.
*
*/
#include "mergegroupscommand.h"
#include "counttable.h"
#include "removeseqscommand.h"
//**********************************************************************************************************************
vector<string> MergeGroupsCommand::setParameters(){
try {
CommandParameter pshared("shared", "InputTypes", "", "", "none", "sharedGroup", "none","shared",false,false,true); parameters.push_back(pshared);
CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup", "sharedGroup", "none","group",false,false,true); parameters.push_back(pgroup);
CommandParameter pcount("count", "InputTypes", "", "", "CountGroup", "sharedGroup", "countfasta","count",false,false,true); parameters.push_back(pcount);
CommandParameter pdesign("design", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(pdesign);
CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "countfasta","fasta",false,false,true); parameters.push_back(pfasta);
CommandParameter pmethod("method", "Multiple", "sum-average-median", "sum", "", "", "","",false,false, true); parameters.push_back(pmethod);
CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
CommandParameter pgroups("groups", "String", "", "", "", "", "","",false,false); parameters.push_back(pgroups);
CommandParameter pseed("seed", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pseed);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
abort = false; calledHelp = false; allLines = true;
vector<string> tempOutNames;
outputTypes["shared"] = tempOutNames;
outputTypes["group"] = tempOutNames;
outputTypes["count"] = tempOutNames;
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string MergeGroupsCommand::getHelpString(){
try {
string helpString = "";
helpString += "The merge.groups command input files are shared, group, count, fasta and a design file. It reads the design file and merges the groups in the other files accordingly.\n";
helpString += "The design parameter allows you to assign your groups to sets. It is required. \n";
helpString += "The fasta parameter allows you to provide a fasta file associated with your count file. This is used if you are using the median method, so that sequences that are entirely removed from the counttable will also be removed from the fasta file. \n";
helpString += "The groups parameter allows you to specify which of the groups in your shared or group file you would like included. The group names are separated by dashes. By default all groups are selected.\n";
helpString += "The label parameter allows you to select what distance levels you would like, and are also separated by dashes.\n";
helpString += "The groups parameter allows you to select groups you would like, and are also separated by dashes.\n";
helpString += "The method parameter allows you to select method you would like to use to merge the groups. Options are sum, average and median. Default=sum.\n";
helpString += "The merge.groups command should be in the following format: merge.groups(design=yourDesignFile, shared=yourSharedFile).\n";
helpString += "Example merge.groups(design=temp.design, groups=A-B-C, shared=temp.shared).\n";
helpString += "The default value for groups is all the groups in your sharedfile, and all labels in your inputfile will be used.\n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string MergeGroupsCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "shared") { pattern = "[filename],merge,[extension]"; }
else if (type == "group") { pattern = "[filename],merge,[extension]"; }
else if (type == "count") { pattern = "[filename],merge,[extension]"; }
else if (type == "fasta") { pattern = "[filename],merge,[extension]"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->setControl_pressed(true); }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
MergeGroupsCommand::MergeGroupsCommand(string option) : Command() {
try {
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else if(option == "category") { abort = true; calledHelp = true; }
else {
OptionParser parser(option, setParameters());
map<string,string> parameters = parser.getParameters();
ValidParameters validParameter;
//check for required parameters
designfile = validParameter.validFile(parameters, "design");
if (designfile == "not open") { abort = true; }
else if (designfile == "not found") {
//if there is a current shared file, use it
designfile = current->getDesignFile();
if (designfile != "") { m->mothurOut("Using " + designfile + " as input file for the design parameter.\n"); }
else { m->mothurOut("You have no current designfile and the design parameter is required.\n"); abort = true; }
}else { current->setDesignFile(designfile); }
sharedfile = validParameter.validFile(parameters, "shared");
if (sharedfile == "not open") { abort = true; sharedfile = ""; }
else if (sharedfile == "not found") { sharedfile = ""; }
else { current->setSharedFile(sharedfile); }
groupfile = validParameter.validFile(parameters, "group");
if (groupfile == "not open") { abort = true; groupfile = ""; }
else if (groupfile == "not found") { groupfile = ""; }
else { current->setGroupFile(groupfile); }
countfile = validParameter.validFile(parameters, "count");
if (countfile == "not open") { abort = true; countfile = ""; }
else if (countfile == "not found") { countfile = ""; }
else { current->setCountFile(countfile); }
fastafile = validParameter.validFile(parameters, "fasta");
if (fastafile == "not open") { abort = true; countfile = ""; }
else if (fastafile == "not found") { fastafile = ""; }
else { current->setFastaFile(fastafile); }
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
label = validParameter.valid(parameters, "label");
if (label == "not found") { label = ""; }
else {
if(label != "all") { util.splitAtDash(label, labels); allLines = false; }
else { allLines = true; }
}
groups = validParameter.valid(parameters, "groups");
if (groups == "not found") { groups = "all"; }
util.splitAtDash(groups, Groups);
if (Groups.size() != 0) { if (Groups[0]== "all") { Groups.clear(); } }
method = validParameter.valid(parameters, "method"); if(method == "not found"){ method = "sum"; }
if ((method != "sum") && (method != "average") && (method != "median")) { m->mothurOut(method + " is not a valid method. Options are sum, average and median. I will use sum.\n"); method = "sum"; }
if ((groupfile != "") && (countfile != "")) {
m->mothurOut("[ERROR]: you may only use one of the following: group or count.\n"); abort=true;
}
if ((sharedfile == "") && (groupfile == "") && (countfile == "")) {
//give priority to group, then shared
groupfile = current->getGroupFile();
if (groupfile != "") { m->mothurOut("Using " + groupfile + " as input file for the group parameter.\n"); }
else {
sharedfile = current->getSharedFile();
if (sharedfile != "") { m->mothurOut("Using " + sharedfile + " as input file for the shared parameter.\n"); }
else {
countfile = current->getCountFile();
if (countfile != "") { m->mothurOut("Using " + countfile + " as input file for the count parameter.\n"); }
else {
m->mothurOut("You have no current groupfile, countfile or sharedfile and one is required.\n"); abort = true;
}
}
}
}
if ((countfile == "") && (fastafile != "")) { m->mothurOut("[ERROR]: You may only use the fasta file with the count file, quitting.\n"); abort=true; }
else if ((countfile != "") && (method == "average")) { m->mothurOut("You may not use the average method with the count file. I will use the sum method.\n"); method = "sum"; }
else if ((countfile != "") && (method == "median") && (fastafile == "")) {
fastafile = current->getFastaFile();
if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter.\n"); }
else {
m->mothurOut("[ERROR]: Fasta file is required with the median method and a count file so that sequences removed from your count table can also be removed from your fasta file to avoid downstream file mismatches, quitting.\n"); abort=true;
}
}
}
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "MergeGroupsCommand");
exit(1);
}
}
//**********************************************************************************************************************
int MergeGroupsCommand::execute(){
try {
if (abort) { if (calledHelp) { return 0; } return 2; }
designMap = new DesignMap(designfile); if (m->getControl_pressed()) { delete designMap; return 0; }
if (method != "sum") {
string defaultClass = designMap->getDefaultClass();
vector<string> treatments = designMap->getCategory(defaultClass);
set<int> numGroupsPerTreatment;
for (int i = 0; i < treatments.size(); i++) {
if (m->getControl_pressed()) { break; }
map<string, vector<string> > checkTreatments;
vector<string> temp; temp.push_back(treatments[i]);
checkTreatments[defaultClass] = temp;
numGroupsPerTreatment.insert(designMap->getNumUnique(checkTreatments));
}
if (numGroupsPerTreatment.size() > 1) { m->mothurOut("[ERROR]: The median and average methods require you to have the same number of sequences in each treatment, quitting.\n"); delete designMap; return 0; }
}
if (groupfile != "") { processGroupFile(designMap); }
if (sharedfile != "") { processSharedFile(designMap); }
if (countfile != "") { processCountFile(designMap); }
//reset groups parameter
delete designMap;
if (m->getControl_pressed()) { for (int i = 0; i < outputNames.size(); i++) { util.mothurRemove(outputNames[i]); } return 0;}
//set shared file as new current sharedfile
string currentName = "";
itTypes = outputTypes.find("shared");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setSharedFile(currentName); }
}
itTypes = outputTypes.find("group");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setGroupFile(currentName); }
}
itTypes = outputTypes.find("count");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setCountFile(currentName); }
}
m->mothurOut("\nOutput File Names: \n");
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i] +"\n"); } m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
int MergeGroupsCommand::process(SharedRAbundVectors*& thisLookUp, ofstream& out, bool& printHeaders){
try {
vector<string> setNames = designMap->getCategory();
//create sharedRabundVectors
vector<SharedRAbundVector*> data = thisLookUp->getSharedRAbundVectors();
//create SharedRAbundVectors for the merged groups. Fill with blank rabundFloatVectors
SharedRAbundVectors* merged; merged = new SharedRAbundVectors();
for (int i = 0; i < setNames.size(); i++) {
SharedRAbundVector* myLookup = new SharedRAbundVector(thisLookUp->getNumBins());
myLookup->setLabel(thisLookUp->getLabel());
myLookup->setGroup(setNames[i]);
merged->push_back(myLookup);
}
//for each OTU
for (int j = 0; j < data[0]->getNumBins(); j++) {
if (m->getControl_pressed()) { break; }
map<string, vector<int> > otusGroupAbunds;
map<string, vector<int> >::iterator itAbunds;
//for each sample
for (int i = 0; i < data.size(); i++) {
string grouping = designMap->get(data[i]->getGroup()); //what set to your belong to
if (grouping == "not found") { m->mothurOut("[ERROR]: " + data[i]->getGroup() + " is not in your design file. Ignoring!\n"); grouping = "NOTFOUND"; }
else {
//Add this OTUs values to sets abunds
itAbunds = otusGroupAbunds.find(grouping);
if (itAbunds == otusGroupAbunds.end()) { //new group
vector<int> temp;
temp.push_back(data[i]->get(j));
otusGroupAbunds[grouping] = temp;
}else {
(itAbunds->second).push_back(data[i]->get(j));
}
}
}
//find results for this bin. Set merged value for this bin in the results
for (itAbunds = otusGroupAbunds.begin(); itAbunds != otusGroupAbunds.end(); itAbunds++) {
int abund = mergeAbund(itAbunds->second);
merged->set(j, abund, itAbunds->first);
}
}
//free memory
for (int i = 0; i < data.size(); i++) { delete data[i]; }
if (m->getControl_pressed()) { delete merged; return 0; }
merged->eliminateZeroOTUS(); // remove any zero OTUs created by median option.
//print new file
merged->print(out, printHeaders);
delete merged;
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "process");
exit(1);
}
}
//**********************************************************************************************************************
int MergeGroupsCommand::processSharedFile(DesignMap*& designMap){
try {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(sharedfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(sharedfile));
variables["[extension]"] = util.getExtension(sharedfile);
string outputFileName = getOutputFileName("shared", variables);
outputTypes["shared"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream out;
util.openOutputFile(outputFileName, out);
InputData input(sharedfile, "sharedfile", Groups);
set<string> processedLabels;
set<string> userLabels = labels;
string lastLabel = "";
SharedRAbundVectors* lookup = util.getNextShared(input, allLines, userLabels, processedLabels, lastLabel);
Groups = lookup->getNamesGroups();
bool printHeaders = true;
while (lookup != nullptr) {
if (m->getControl_pressed()) { delete lookup; break; }
process(lookup, out, printHeaders); delete lookup;
lookup = util.getNextShared(input, allLines, userLabels, processedLabels, lastLabel);
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "processSharedFile");
exit(1);
}
}
//**********************************************************************************************************************
int MergeGroupsCommand::processGroupFile(DesignMap*& designMap){
try {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(groupfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(groupfile));
variables["[extension]"] = util.getExtension(groupfile);
string outputFileName = getOutputFileName("group", variables);
outputTypes["group"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream out;
util.openOutputFile(outputFileName, out);
//read groupfile
GroupMap groupMap(groupfile);
groupMap.readMap();
vector<string> nameGroups = groupMap.getNamesOfGroups();
if (Groups.size() == 0) { Groups = nameGroups; }
vector<string> namesOfSeqs = groupMap.getNamesSeqs();
bool error = false;
for (int i = 0; i < namesOfSeqs.size(); i++) {
if (m->getControl_pressed()) { break; }
string thisGroup = groupMap.getGroup(namesOfSeqs[i]);
//are you in a group the user wants
if (util.inUsersGroups(thisGroup, Groups)) {
string thisGrouping = designMap->get(thisGroup);
if (thisGrouping == "not found") { m->mothurOut("[ERROR]: " + namesOfSeqs[i] + " is from group " + thisGroup + " which is not in your design file, please correct.\n"); error = true; }
else {
out << namesOfSeqs[i] << '\t' << thisGrouping << endl;
}
}
}
if (error) { m->setControl_pressed(true); }
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "processGroupFile");
exit(1);
}
}
//**********************************************************************************************************************
int MergeGroupsCommand::processCountFile(DesignMap*& designMap){
try {
CountTable countTable;
if (!countTable.testGroups(countfile)) { m->mothurOut("[ERROR]: your countfile contains no group information, please correct.\n"); m->setControl_pressed(true); return 0; }
//read countTable
countTable.readTable(countfile, true, false);
//fill Groups - checks for "all" and for any typo groups
vector<string> nameGroups = countTable.getNamesOfGroups();
if (Groups.size() == 0) { Groups = nameGroups; }
vector<string> dnamesGroups = designMap->getNamesGroups();
//sanity check
bool error = false;
if (nameGroups.size() == dnamesGroups.size()) { //at least there are the same number
//is every group in counttable also in designmap
for (int i = 0; i < nameGroups.size(); i++) {
if (m->getControl_pressed()) { break; }
if (!util.inUsersGroups(nameGroups[i], dnamesGroups)) { error = true; break; }
}
}
if (error) { m->mothurOut("[ERROR]: Your countfile does not contain the same groups as your design file, please correct\n"); m->setControl_pressed(true); return 0; }
//user selected groups - remove some groups from table
if (Groups.size() != nameGroups.size()) {
for (int i = 0; i < nameGroups.size(); i++) {
if (!util.inUsersGroups(nameGroups[i], Groups)) { countTable.removeGroup(nameGroups[i]); }
}
}
//ask again in case order changed
nameGroups = countTable.getNamesOfGroups();
int numGroups = nameGroups.size();
//create new table
CountTable newTable;
vector<string> treatments = designMap->getCategory();
map<string, vector<int> > clearedMap;
for (int i = 0; i < treatments.size(); i++) {
newTable.addGroup(treatments[i]);
vector<int> temp;
clearedMap[treatments[i]] = temp;
}
treatments = newTable.getNamesOfGroups();
set<string> namesToRemove;
vector<string> namesOfSeqs = countTable.getNamesOfSeqs();
for (int i = 0; i < namesOfSeqs.size(); i++) {
if (m->getControl_pressed()) { break; }
vector<int> thisSeqsCounts = countTable.getGroupCounts(namesOfSeqs[i]);
map<string, vector<int> > thisSeqsMap = clearedMap;
for (int j = 0; j < numGroups; j++) {
thisSeqsMap[designMap->get(nameGroups[j])].push_back(thisSeqsCounts[j]);
}
//create new counts for seq for new table
vector<int> newCounts; int totalAbund = 0;
for (int j = 0; j < treatments.size(); j++){
int abund = mergeAbund(thisSeqsMap[treatments[j]]);
newCounts.push_back(abund); //order matters, add in count for each treatment in new table.
totalAbund += abund;
}
//add seq to new table
if(totalAbund == 0) {
namesToRemove.insert(namesOfSeqs[i]);
}else { newTable.push_back(namesOfSeqs[i], newCounts); }
}
if (error) { m->setControl_pressed(true); return 0; }
//remove sequences zeroed out by median method
if (namesToRemove.size() != 0) {
//print names
ofstream out;
string accnosFile = "accnosFile.temp";
util.openOutputFile(accnosFile, out);
//output to .accnos file
for (set<string>::iterator it = namesToRemove.begin(); it != namesToRemove.end(); it++) {
if (m->getControl_pressed()) { out.close(); util.mothurRemove(accnosFile); return 0; }
out << *it << endl;
}
out.close();
//run remove.seqs
string inputString = "accnos=" + accnosFile + ", fasta=" + fastafile;
m->mothurOut("/******************************************/\n");
m->mothurOut("Running command: remove.seqs(" + inputString + ")\n");
current->setMothurCalling(true);
Command* removeCommand = new RemoveSeqsCommand(inputString);
removeCommand->execute();
map<string, vector<string> > filenames = removeCommand->getOutputFiles();
delete removeCommand;
current->setMothurCalling(false);
m->mothurOut("/******************************************/\n");
util.mothurRemove(accnosFile);
}
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(countfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(countfile));
variables["[extension]"] = util.getExtension(countfile);
string outputFileName = getOutputFileName("count", variables);
outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
newTable.printTable(outputFileName);
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "processCountFile");
exit(1);
}
}
//**********************************************************************************************************************
int MergeGroupsCommand::mergeAbund(vector<int> values){
try {
int abund = 0;
if (method == "sum") {
abund = util.sum(values);
}else if (method == "average") {
abund = util.average(values);
}else if (method == "median") {
abund = util.median(values);
}else {
m->mothurOut("[ERROR]: Invalid method. \n"); m->setControl_pressed(true); return 0;
}
return abund;
}
catch(exception& e) {
m->errorOut(e, "MergeGroupsCommand", "mergeAbund");
exit(1);
}
}
//**********************************************************************************************************************
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