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//
// mergeotuscommand.cpp
// Mothur
//
// Created by Sarah Westcott on 12/10/18.
// Copyright © 2018 Schloss Lab. All rights reserved.
//
#include "mergeotuscommand.hpp"
//**********************************************************************************************************************
vector<string> MergeOTUsCommand::setParameters(){
try {
CommandParameter pconstaxonomy("constaxonomy", "InputTypes", "", "", "none", "none", "none","constaxonomy",false,true, true); parameters.push_back(pconstaxonomy);
CommandParameter pshared("shared", "InputTypes", "", "", "none", "none", "none","shared",false,true,true); parameters.push_back(pshared);
CommandParameter prelabund("relabund", "InputTypes", "", "", "none", "none", "none","relabund",false,true,true); parameters.push_back(prelabund);
CommandParameter plist("list", "InputTypes", "", "", "none", "none", "none","list",false,true,true); parameters.push_back(plist);
CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
CommandParameter ptaxlevel("taxlevel", "Number", "", "-1", "", "", "","",false,false,true); parameters.push_back(ptaxlevel);
CommandParameter pseed("seed", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pseed);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
abort = false; calledHelp = false; allLines = true;
vector<string> tempOutNames;
outputTypes["shared"] = tempOutNames;
outputTypes["list"] = tempOutNames;
outputTypes["relabund"] = tempOutNames;
outputTypes["constaxonomy"] = tempOutNames;
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string MergeOTUsCommand::getHelpString(){
try {
string helpString = "";
helpString += "The merge.otus command parameters are shared, list, relabund, constaxonomy, taxlevel and label. constaxonomy is a required, unless you have a valid current file.\n";
helpString += "The taxlevel parameter allows you to specify the taxonomy level you would like to use when merging. Default=maxlevel.\n";
helpString += "Example merge.otus(shared=yourSharedFile, constaxonomy=yourConsTaxonomyFile).\n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string MergeOTUsCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "shared") { pattern = "[filename],merge,[extension]"; }
else if (type == "list") { pattern = "[filename],merge,[extension]"; }
else if (type == "relabund") { pattern = "[filename],merge,[extension]"; }
else if (type == "constaxonomy") { pattern = "[filename],[label],merge,cons.taxonomy"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->setControl_pressed(true); }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
MergeOTUsCommand::MergeOTUsCommand(string option) : Command() {
try {
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else if(option == "category") { abort = true; calledHelp = true; }
else {
OptionParser parser(option, setParameters());
map<string,string> parameters = parser.getParameters();
ValidParameters validParameter;
sharedfile = validParameter.validFile(parameters, "shared");
if (sharedfile == "not found") { sharedfile = ""; }
else if (sharedfile == "not open") { sharedfile = ""; abort = true; }
else { current->setSharedFile(sharedfile); }
listfile = validParameter.validFile(parameters, "list");
if (listfile == "not found") { listfile = ""; }
else if (listfile == "not open") { listfile = ""; abort = true; }
else { current->setListFile(listfile); }
relabundfile = validParameter.validFile(parameters, "relabund");
if (relabundfile == "not found") { relabundfile = ""; }
else if (relabundfile == "not open") { relabundfile = ""; abort = true; }
else { current->setRelAbundFile(relabundfile); }
constaxfile = validParameter.validFile(parameters, "constaxonomy"); //required
if (constaxfile == "not found") {
constaxfile = current->getConsTaxonomyFile();
if (constaxfile != "") { m->mothurOut("Using " + constaxfile + " as input file for the constaxonomy parameter.\n"); }
else { m->mothurOut("[ERROR]: You have no current constaxonomy file and the constaxonomy parameter is required.\n"); abort = true; }
}
else if (constaxfile == "not open") { constaxfile = ""; abort = true; }
else { current->setConsTaxonomyFile(constaxfile); }
if ((relabundfile == "") && (listfile == "") && (sharedfile == "")) { //no files to merge provided, look for currents
//is there are current file available for any of these?
//give priority to shared, then list, then relabund
//if there is a current shared file, use it
sharedfile = current->getSharedFile();
if (sharedfile != "") { m->mothurOut("Using " + sharedfile + " as input file for the shared parameter.\n"); }
else {
listfile = current->getListFile();
if (listfile != "") { m->mothurOut("Using " + listfile + " as input file for the list parameter.\n"); }
else {
relabundfile = current->getRelAbundFile();
if (relabundfile != "") { m->mothurOut("Using " + relabundfile + " as input file for the rabund parameter.\n"); }
else {
m->mothurOut("[ERROR]: No valid current files. You must provide a list, relabund or shared file.\n"); abort = true;
}
}
}
}
if (outputdir == ""){
outputdir += util.hasPath(constaxfile);
}
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
label = validParameter.valid(parameters, "label");
if (label == "not found") { label = ""; }
else {
if(label != "all") { util.splitAtDash(label, labels); allLines = false; }
else { allLines = true; }
}
string temp = validParameter.valid(parameters, "taxlevel"); if (temp == "not found") { temp = "-1"; }
util.mothurConvert(temp, taxLevelCutoff); //-1 means use max level
}
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "MergeOTUsCommand");
exit(1);
}
}
//**********************************************************************************************************************
MergeOTUsCommand::~MergeOTUsCommand(){}
//**********************************************************************************************************************
int MergeOTUsCommand::execute(){
try {
if (abort) { if (calledHelp) { return 0; } return 2; }
//read in consensus taxonomy
vector<consTax> conTaxes = util.readConsTax(constaxfile);
PhyloTree tree;
//add consensus taxonomy to phylo tree
for (size_t i = 0; i < conTaxes.size(); i++) {
tree.addSeqToTree(conTaxes[i].name, conTaxes[i].taxonomy);
}
//build tree
tree.assignHeirarchyIDs(0);
//get max level of phylotree
int maxlevel = tree.getMaxLevel();
//is the taxlevel parameter valid - not greater than the max level in the file. If greater reduce to maxlevel.
if (taxLevelCutoff == -1) { //default, use maxlevel
taxLevelCutoff = maxlevel;
}else if ( taxLevelCutoff > maxlevel) { //invalid taxlevel, use maxlevel
m->mothurOut("[WARNING]: The taxlevel selected is larger than the maxlevel in your constaxonomy file, disregarding. Using the max level of " + toString(maxlevel) + " for the taxlevel parameter.\n");
taxLevelCutoff = maxlevel;
}
for (size_t i = 0; i < conTaxes.size(); i++) {
string otuTax = conTaxes[i].taxonomy;
if (taxLevelCutoff != maxlevel) { otuTax = util.trimTax(otuTax, taxLevelCutoff); }
otuLabel2ConsTax[conTaxes[i].name] = otuTax;
if (listfile != "") { otuLabel2ConsSize[conTaxes[i].name] = conTaxes[i].abundance; }
}
conTaxes.clear();
//extract tree nodes at taxlevel
vector<TaxNode> thisLevelsNodes = tree.getNodes(taxLevelCutoff);
//merge otus at each node at this level
if (listfile != "") { mergeListOTUs(thisLevelsNodes); }
else if (sharedfile != "") { mergeSharedOTUs(thisLevelsNodes); }
else if (relabundfile != "") { mergeRelabundOTUs(thisLevelsNodes); }
if (m->getControl_pressed()) { for (int i = 0; i < outputNames.size(); i++) { util.mothurRemove(outputNames[i]); } return 0; }
string currentName = "";
itTypes = outputTypes.find("list");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setListFile(currentName); }
}
itTypes = outputTypes.find("shared");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setSharedFile(currentName); }
}
itTypes = outputTypes.find("relabund");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setRelAbundFile(currentName); }
}
//set constaxonomy file as new current constaxonomyfile
itTypes = outputTypes.find("constaxonomy");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setConsTaxonomyFile(currentName); }
}
m->mothurOut("\nOutput File Names:\n");
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); } m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
int MergeOTUsCommand::mergeSharedOTUs(vector<TaxNode>& nodes){
try {
string numNodes = toString(nodes.size());
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(sharedfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(sharedfile));
variables["[extension]"] = util.getExtension(sharedfile);
string outputFileName = getOutputFileName("shared", variables);
outputTypes["shared"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream out;
util.openOutputFile(outputFileName, out);
InputData input(sharedfile, "sharedfile", Groups);
set<string> processedLabels;
set<string> userLabels = labels;
string lastLabel = "";
SharedRAbundVectors* lookup = util.getNextShared(input, allLines, userLabels, processedLabels, lastLabel);
Groups = lookup->getNamesGroups();
bool printHeaders = true;
while (lookup != nullptr) {
if (m->getControl_pressed()) { delete lookup; break; }
process(lookup, out, printHeaders, nodes); delete lookup;
lookup = util.getNextShared(input, allLines, userLabels, processedLabels, lastLabel);
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "mergeSharedOTUs");
exit(1);
}
}
//**********************************************************************************************************************
int MergeOTUsCommand::process(SharedRAbundVectors*& thisLookUp, ofstream& out, bool& printHeaders, vector<TaxNode>& nodes){
try {
vector<string> groups = thisLookUp->getNamesGroups();
//create SharedRAbundVectors for the merged groups. Fill with blank rabundFloatVectors
SharedRAbundVectors* merged; merged = new SharedRAbundVectors();
for (int i = 0; i < groups.size(); i++) {
SharedRAbundVector* myLookup = new SharedRAbundVector();
myLookup->setLabel(thisLookUp->getLabel());
myLookup->setGroup(groups[i]);
merged->push_back(myLookup);
}
//translate otuNames to bin numbers
map<string, int> otuName2BinNumber;
map<string, int>::iterator it;
for (int j = 0; j < thisLookUp->getNumBins(); j++) {
if (m->getControl_pressed()) { break; }
otuName2BinNumber[thisLookUp->getOTUName(j)] = j;
}
if (m->getControl_pressed()) { delete merged; return 0; }
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(constaxfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(constaxfile));
variables["[label]"] = thisLookUp->getLabel();
string outputFileName = getOutputFileName("constaxonomy", variables);
outputTypes["constaxonomy"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream outCons;
util.openOutputFile(outputFileName, outCons);
outCons << "OTU\tSize\tTaxonomy\n";
for (int i = 0; i < nodes.size(); i++) {
if (m->getControl_pressed()) { break; }
vector<string> otuNames = nodes[i].accessions; //otus to merge
string newOTUName = otuNames[0];
vector<int> abunds; abunds.resize(groups.size(), 0);
for (int j = 0; j < otuNames.size(); j++) {
it = otuName2BinNumber.find(otuNames[j]); //do we have this otu in the shared file
if (it != otuName2BinNumber.end()) { //we found it
vector<int> thisOtusAbunds = thisLookUp->getOTU(it->second);
for (int k = 0; k < thisOtusAbunds.size(); k++) { abunds[k] += thisOtusAbunds[k]; } //add this otus abunds to merged otu abunds
}else { m->mothurOut("[ERROR]: missing otu " + otuNames[j] + " from shared file, cannot continue.\n"); m->setControl_pressed(true); break; }
}
merged->push_back(abunds, newOTUName);
//merge consensus taxonomy results
int sumOtu = util.sum(abunds);
outCons << newOTUName << '\t' << sumOtu << '\t' << otuLabel2ConsTax[newOTUName] << endl;
}
if (m->getControl_pressed()) { delete merged; return 0; }
merged->eliminateZeroOTUS(); // remove any zero OTUs created by median option.
//print new file
merged->print(out, printHeaders);
delete merged;
outCons.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "process");
exit(1);
}
}
//**********************************************************************************************************************
int MergeOTUsCommand::mergeListOTUs(vector<TaxNode>& nodes){
try {
string numNodes = toString(nodes.size());
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(listfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(listfile));
variables["[extension]"] = util.getExtension(listfile);
string outputFileName = getOutputFileName("list", variables);
outputTypes["list"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream out;
util.openOutputFile(outputFileName, out);
InputData input(listfile, "list", Groups);
set<string> processedLabels;
set<string> userLabels = labels;
string lastLabel = "";
ListVector* list = util.getNextList(input, allLines, userLabels, processedLabels, lastLabel);
bool printHeaders = true;
while (list != nullptr) {
if (m->getControl_pressed()) { delete list; break; }
process(list, out, printHeaders, nodes); delete list;
list = util.getNextList(input, allLines, userLabels, processedLabels, lastLabel);
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "mergeListOTUs");
exit(1);
}
}
//**********************************************************************************************************************
int MergeOTUsCommand::process(ListVector*& list, ofstream& out, bool& printHeaders, vector<TaxNode>& nodes){
try {
ListVector* merged; merged = new ListVector();
merged->setLabel(list->getLabel());
//translate otuNames to bin numbers
map<string, int> otuName2BinNumber;
map<string, int>::iterator it;
for (int j = 0; j < list->getNumBins(); j++) {
if (m->getControl_pressed()) { break; }
otuName2BinNumber[list->getOTUName(j)] = j;
}
if (m->getControl_pressed()) { delete merged; return 0; }
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(constaxfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(constaxfile));
variables["[label]"] = list->getLabel();
string outputFileName = getOutputFileName("constaxonomy", variables);
outputTypes["constaxonomy"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream outCons;
util.openOutputFile(outputFileName, outCons);
outCons << "OTU\tSize\tTaxonomy\n";
for (int i = 0; i < nodes.size(); i++) {
if (m->getControl_pressed()) { break; }
vector<string> otuNames = nodes[i].accessions; //otus to merge
string newOTUName = otuNames[0];
string mergedNames = "";
it = otuName2BinNumber.find(newOTUName); //do we have this otu in the shared file
int sizeOtu = 0;
if (it != otuName2BinNumber.end()) { //we found it
mergedNames = list->get(it->second);
sizeOtu += otuLabel2ConsSize[newOTUName];
}else { m->mothurOut("[ERROR]: missing otu " + newOTUName + " from list file, cannot continue.\n"); m->setControl_pressed(true); }
for (int j = 1; j < otuNames.size(); j++) {
it = otuName2BinNumber.find(otuNames[j]); //do we have this otu in the shared file
if (it != otuName2BinNumber.end()) { //we found it
string bin = list->get(it->second);
mergedNames += "," + bin;
sizeOtu += otuLabel2ConsSize[otuNames[j]];
}else { m->mothurOut("[ERROR]: missing otu " + otuNames[j] + " from list file, cannot continue.\n"); m->setControl_pressed(true); break; }
}
int sumOtu = util.getNumNames(mergedNames);
merged->push_back(mergedNames, sumOtu, newOTUName);
outCons << newOTUName << '\t' << sizeOtu << '\t' << otuLabel2ConsTax[newOTUName] << endl;
}
if (m->getControl_pressed()) { delete merged; return 0; }
//print new file
merged->print(out, printHeaders);
delete merged;
outCons.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "process");
exit(1);
}
}
//**********************************************************************************************************************
int MergeOTUsCommand::mergeRelabundOTUs(vector<TaxNode>& nodes){
try {
string numNodes = toString(nodes.size());
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(relabundfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(relabundfile));
variables["[extension]"] = util.getExtension(relabundfile);
string outputFileName = getOutputFileName("relabund", variables);
outputTypes["relabund"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream out; util.openOutputFile(outputFileName, out);
InputData input(relabundfile, "relabund", Groups);
set<string> processedLabels;
set<string> userLabels = labels;
string lastLabel = "";
SharedRAbundFloatVectors* lookup = util.getNextRelabund(input, allLines, userLabels, processedLabels, lastLabel);
Groups = lookup->getNamesGroups();
bool printHeaders = true;
while (lookup != nullptr) {
if (m->getControl_pressed()) { delete lookup; break; }
process(lookup, out, printHeaders, nodes); delete lookup;
lookup = util.getNextRelabund(input, allLines, userLabels, processedLabels, lastLabel);
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "mergeRelabundOTUs");
exit(1);
}
}
//**********************************************************************************************************************
int MergeOTUsCommand::process(SharedRAbundFloatVectors*& thisLookUp, ofstream& out, bool& printHeaders, vector<TaxNode>& nodes){
try {
vector<string> groups = thisLookUp->getNamesGroups();
//create SharedRAbundVectors for the merged groups. Fill with blank rabundFloatVectors
SharedRAbundFloatVectors* merged; merged = new SharedRAbundFloatVectors();
for (int i = 0; i < groups.size(); i++) {
SharedRAbundFloatVector* myLookup = new SharedRAbundFloatVector();
myLookup->setLabel(thisLookUp->getLabel());
myLookup->setGroup(groups[i]);
merged->push_back(myLookup);
}
//translate otuNames to bin numbers
map<string, int> otuName2BinNumber;
map<string, int>::iterator it;
for (int j = 0; j < thisLookUp->getNumBins(); j++) {
if (m->getControl_pressed()) { break; }
otuName2BinNumber[thisLookUp->getOTUName(j)] = j;
}
if (m->getControl_pressed()) { delete merged; return 0; }
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(constaxfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(constaxfile));
variables["[label]"] = thisLookUp->getLabel();
string outputFileName = getOutputFileName("constaxonomy", variables);
outputTypes["constaxonomy"].push_back(outputFileName); outputNames.push_back(outputFileName);
ofstream outCons;
util.openOutputFile(outputFileName, outCons);
outCons << "OTU\tSize\tTaxonomy\n";
for (int i = 0; i < nodes.size(); i++) {
if (m->getControl_pressed()) { break; }
vector<string> otuNames = nodes[i].accessions; //otus to merge
string newOTUName = otuNames[0];
vector<float> abunds; abunds.resize(groups.size(), 0);
for (int j = 0; j < otuNames.size(); j++) {
it = otuName2BinNumber.find(otuNames[j]); //do we have this otu in the shared file
if (it != otuName2BinNumber.end()) { //we found it
vector<float> thisOtusAbunds = thisLookUp->getOTU(it->second);
for (int k = 0; k < thisOtusAbunds.size(); k++) { abunds[k] += thisOtusAbunds[k]; } //add this otus abunds to merged otu abunds
}else { m->mothurOut("[ERROR]: missing otu " + otuNames[j] + " from relabund file, cannot continue.\n"); m->setControl_pressed(true); break; }
}
merged->push_back(abunds, newOTUName);
//merge consensus taxonomy results
float sumOtu = util.sum(abunds);
outCons << newOTUName << '\t' << sumOtu << '\t' << otuLabel2ConsTax[newOTUName] << endl;
}
if (m->getControl_pressed()) { delete merged; return 0; }
merged->eliminateZeroOTUS(); // remove any zero OTUs created by median option.
//print new file
merged->print(out, printHeaders);
delete merged;
outCons.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeOTUsCommand", "process");
exit(1);
}
}
//**********************************************************************************************************************
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