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|
//
// renameseqscommand.cpp
// Mothur
//
// Created by SarahsWork on 5/28/13.
// Copyright (c) 2013 Schloss Lab. All rights reserved.
//
#include "renameseqscommand.h"
#include "sequence.hpp"
#include "groupmap.h"
#include "counttable.h"
#include "qualityscores.h"
#include "contigsreport.hpp"
#include "inputdata.h"
#include "fastqread.h"
//**********************************************************************************************************************
vector<string> RenameSeqsCommand::setParameters(){
try {
CommandParameter pfile("file", "InputTypes", "", "", "fileFasta-file", "fileFasta", "none","fasta",false,false,true); parameters.push_back(pfile);
CommandParameter pmap("map", "InputTypes", "", "", "none", "none", "none","fasta",false,false,true); parameters.push_back(pmap);
CommandParameter pfasta("fasta", "InputTypes", "", "", "fileFasta-file", "fileFasta", "none","fasta",false,false,true); parameters.push_back(pfasta);
CommandParameter pfastq("fastq", "InputTypes", "", "", "fileFasta-file", "fileFasta", "none","fasta",false,false,true); parameters.push_back(pfastq);
CommandParameter plist("list", "InputTypes", "", "", "fileFasta-file", "fileFasta", "none","fasta",false,false,true); parameters.push_back(plist);
CommandParameter pqfile("qfile", "InputTypes", "", "", "file", "none", "none","qfile",false,false,true); parameters.push_back(pqfile);
CommandParameter pcontigsreport("contigsreport", "InputTypes", "", "", "file", "none", "none","contigsreport",false,false,true); parameters.push_back(pcontigsreport);
CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "file", "none","taxonomy",false,false,true); parameters.push_back(ptaxonomy);
CommandParameter pname("name", "InputTypes", "", "", "NameCount-file", "none", "none","name",false,false,true); parameters.push_back(pname);
CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup-file", "none", "none","count",false,false,true); parameters.push_back(pcount);
CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup-file", "none", "none","group",false,false,true); parameters.push_back(pgroup);
CommandParameter pdelim("delim", "String", "", "_", "", "", "","",false,false); parameters.push_back(pdelim);
CommandParameter pplacement("placement", "Multiple", "front-back", "back", "", "", "","",false,false); parameters.push_back(pplacement);
CommandParameter pseed("seed", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pseed);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
abort = false; calledHelp = false;
vector<string> tempOutNames;
outputTypes["list"] = tempOutNames;
outputTypes["fasta"] = tempOutNames;
outputTypes["name"] = tempOutNames;
outputTypes["group"] = tempOutNames;
outputTypes["count"] = tempOutNames;
outputTypes["map"] = tempOutNames;
outputTypes["qfile"] = tempOutNames;
outputTypes["file"] = tempOutNames;
outputTypes["fastq"] = tempOutNames;
outputTypes["contigsreport"] = tempOutNames;
outputTypes["taxonomy"] = tempOutNames;
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string RenameSeqsCommand::getHelpString(){
try {
string helpString = "";
helpString += "The rename.seqs command renames sequences in the input files. By default, mothur will generate new names based on your inputs. Alternatively, you can provide a map file.\n";
helpString += "The rename.seqs command parameters are " + getCommandParameters() + ".\n";
helpString += "The list parameter allows you to provide an associated list file.\n";
helpString += "The fasta parameter allows you to provide an associated fasta file.\n";
helpString += "The qfile parameter allows you to provide an associated quality file.\n";
helpString += "The taxonomy parameter allows you to provide an associated taxonomy file.\n";
helpString += "The contigsreport allows you to provide an associated contigsreport file.\n";
helpString += "The file parameter is 2, 3 or 4 column file containing the forward fastq files in the first column and their matching reverse fastq files in the second column, or a groupName then forward fastq file and reverse fastq file, or forward fastq file then reverse fastq then forward index and reverse index file. If you only have one index file add 'none' for the other one. Mothur will process each pair and create a renamed fastq and file file.\n";
helpString += "The placement parameter allows you to indicate whether you would like the group name appended to the front or back of the sequence number. Options are front or back. Default=back.\n";
helpString += "The delim parameter allow you to enter the character or characters you would like to separate the sequence number from the group name. Default='_'.\n";
helpString += "The rename.seqs command should be in the following format: \n";
helpString += "The rename.seqs command should be in the following format: \n";
helpString += "rename.seqs(fasta=yourFastaFile, group=yourGroupFile) \n";
helpString += "Example rename.seqs(fasta=abrecovery.unique.fasta, group=abrecovery.group).\n";
;
return helpString;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string RenameSeqsCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "fasta") { pattern = "[filename],renamed,[extension]"; }
else if (type == "name") { pattern = "[filename],renamed,[extension]"; }
else if (type == "group") { pattern = "[filename],renamed,[extension]"; }
else if (type == "count") { pattern = "[filename],renamed,[extension]"; }
else if (type == "taxonomy") { pattern = "[filename],renamed,[extension]"; }
else if (type == "qfile") { pattern = "[filename],renamed,[extension]"; }
else if (type == "fastq") { pattern = "[filename],renamed,[extension]"; }
else if (type == "file") { pattern = "[filename],renamed,[extension]"; }
else if (type == "contigsreport") { pattern = "[filename],renamed,[extension]"; }
else if (type == "list") { pattern = "[filename],renamed,[extension]"; }
else if (type == "map") { pattern = "[filename],renamed_map"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->setControl_pressed(true); }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "getOutputPattern");
exit(1);
}
}
/**************************************************************************************/
RenameSeqsCommand::RenameSeqsCommand(string option) : Command() {
try {
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else if(option == "category") { abort = true; calledHelp = true; }
else {
OptionParser parser(option, setParameters());
map<string,string> parameters = parser.getParameters();
ValidParameters validParameter;
//check for required parameters
fastaFile = validParameter.validFile(parameters, "fasta");
if (fastaFile == "not open") { abort = true; }
else if (fastaFile == "not found") { fastaFile = ""; }
else { current->setFastaFile(fastaFile); }
fastqfile = validParameter.validFile(parameters, "fastq");
if (fastqfile == "not open") { abort = true; }
else if (fastqfile == "not found") { fastqfile = ""; }
fileFile = validParameter.validFile(parameters, "file");
if (fileFile == "not open") { abort = true; }
else if (fileFile == "not found") { fileFile = ""; }
else { current->setFileFile(fileFile); }
groupfile = validParameter.validFile(parameters, "group");
if (groupfile == "not open") { abort = true; }
else if (groupfile == "not found") { groupfile = ""; }
else { current->setGroupFile(groupfile); }
countfile = validParameter.validFile(parameters, "count");
if (countfile == "not open") { countfile = ""; abort = true; }
else if (countfile == "not found") { countfile = ""; }
else { current->setCountFile(countfile); }
nameFile = validParameter.validFile(parameters, "name");
if (nameFile == "not open") { abort = true; }
else if (nameFile == "not found"){ nameFile =""; }
else { current->setNameFile(nameFile); }
qualfile = validParameter.validFile(parameters, "qfile");
if (qualfile == "not open") { abort = true; }
else if (qualfile == "not found"){ qualfile =""; }
else { current->setQualFile(qualfile); }
listfile = validParameter.validFile(parameters, "list");
if (listfile == "not open") { abort = true; }
else if (listfile == "not found"){ listfile =""; }
else { current->setListFile(listfile); }
mapFile = validParameter.validFile(parameters, "map");
if (mapFile == "not open") { abort = true; }
else if (mapFile == "not found"){ mapFile = ""; }
contigsfile = validParameter.validFile(parameters, "contigsreport");
if (contigsfile == "not open") { abort = true; }
else if (contigsfile == "not found"){ contigsfile = ""; }
taxfile = validParameter.validFile(parameters, "taxonomy");
if (taxfile == "not open") { taxfile = ""; abort = true; }
else if (taxfile == "not found") { taxfile = ""; }
else { current->setTaxonomyFile(taxfile); }
if ((countfile != "") && (nameFile != "")) { m->mothurOut("[ERROR]: You must enter ONLY ONE of the following: count or name.\n"); abort = true; }
if ((fileFile != "") && (fastaFile != "")) { m->mothurOut("[ERROR]: You must enter ONLY ONE of the following: file or fasta.\n"); abort = true; }
if ((countfile != "") && (groupfile != "")) { m->mothurOut("[ERROR]: You must enter ONLY ONE of the following: count or group.\n"); abort = true; }
if ((fileFile != "") && ((listfile != "") || (nameFile != "") || (fastqfile != "") || (groupfile != "") || (qualfile != "") || (contigsfile != "") || (countfile != "") || (fastaFile != "")) ) {
m->mothurOut("[ERROR]: The file option cannot be used with any other files.\n"); abort = true;
}else if ((fileFile == "") && (listfile == "") && (nameFile == "") && (fastqfile == "") && (groupfile == "") && (qualfile == "") && (contigsfile == "") && (countfile == "") && (fastaFile == "")) {
m->mothurOut("[ERROR]: No input files provided, please correct.\n"); abort = true;
}
placement = validParameter.valid(parameters, "placement"); if (placement == "not found") { placement = "back"; }
if ((placement == "front") || (placement == "back")) { }
else { m->mothurOut("[ERROR]: " + placement + " is not a valid placement option. Valid placement options are front or back.\n"); abort = true; }
delim = validParameter.valid(parameters, "delim"); if (delim == "not found") { delim = "_"; }
}
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "RenameSeqsCommand");
exit(1);
}
}
/**************************************************************************************/
int RenameSeqsCommand::execute() {
try {
if (abort) { if (calledHelp) { return 0; } return 2; }
ignoreNew = false;
map<string, string> renameMap; bool printMap = false;
if (mapFile != "") { readMapFile(renameMap); ignoreNew = true; }
else { printMap = true; }
if (fileFile != "") { processFile(); }
else {
map<string, string> old2NewNameMap;
if ((nameFile != "") || (countfile != "") || (groupfile != "")) {
processNameGroupCountFiles(renameMap, old2NewNameMap);
}else if (mapFile != "") {
old2NewNameMap = renameMap;
}
renameMap.clear();
if (m->getControl_pressed()) { for (int i = 0; i < outputNames.size(); i++) { util.mothurRemove(outputNames[i]); } return 0; }
if (listfile != "") { readList(old2NewNameMap); }
if (fastaFile != "") { readFasta(old2NewNameMap); }
if (fastqfile != "") { readFastq(old2NewNameMap); }
if (qualfile != "") { readQual(old2NewNameMap); }
if (contigsfile != "") { readContigs(old2NewNameMap); }
if (taxfile != "") { readTax(old2NewNameMap); }
if (printMap) {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(fastaFile); }
map<string, string> variables;
string outMapFile = thisOutputDir + util.getRootName(util.getSimpleName(fastaFile));
variables["[filename]"] = outMapFile;
outMapFile = getOutputFileName("map", variables);
outputNames.push_back(outMapFile); outputTypes["map"].push_back(outMapFile);
ofstream outMap; util.openOutputFile(outMapFile, outMap);
//print map
for(map<string, string>::iterator it = old2NewNameMap.begin(); it != old2NewNameMap.end(); it++) {
outMap << it->second << '\t' << it->first << endl;
}
outMap.close();
}
}
if (m->getControl_pressed()) { for (int i = 0; i < outputNames.size(); i++) { util.mothurRemove(outputNames[i]); } return 0; }
m->mothurOut("\nOutput File Names:\n");
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
m->mothurOutEndLine();
//set fasta file as new current fastafile
string currentName = "";
itTypes = outputTypes.find("fasta");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setFastaFile(currentName); }
}
itTypes = outputTypes.find("list");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setListFile(currentName); }
}
itTypes = outputTypes.find("name");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setNameFile(currentName); }
}
itTypes = outputTypes.find("group");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setGroupFile(currentName); }
}
itTypes = outputTypes.find("count");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setCountFile(currentName); }
}
itTypes = outputTypes.find("qfile");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setQualFile(currentName); }
}
itTypes = outputTypes.find("file");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentName = (itTypes->second)[0]; current->setFileFile(currentName); }
}
return 0;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
void RenameSeqsCommand::processNameGroupCountFiles(map<string, string>& oldMap, map<string, string>& old2NewNameMap){
try {
bool oldMapEmpty = true;
if (oldMap.size() != 0) { oldMapEmpty = false; }
GroupMap* groupMap = nullptr;
CountTable* countTable = nullptr;
bool hasGroups = false;
vector<string> Groups;
if (groupfile != "") {
groupMap = new GroupMap(groupfile);
int groupError = groupMap->readMap();
if (groupError == 1) { delete groupMap; return; }
Groups = groupMap->getNamesOfGroups();
hasGroups = true;
}else if (countfile != "") {
countTable = new CountTable();
countTable->readTable(countfile, true, false);
hasGroups = countTable->hasGroupInfo();
if (hasGroups) { Groups = countTable->getNamesOfGroups(); Groups.push_back("Multi"); }
}
//set up for reads
map<string, int> counts; for (int i = 0; i < Groups.size(); i++) { counts[Groups[i]] = 1; }
string thisOutputDir = outputdir;
if (nameFile != "") {
map<string, vector<string> > nameMap;
thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(nameFile); }
string outNameFile = thisOutputDir + util.getRootName(util.getSimpleName(nameFile));
map<string, string> variables;
variables["[filename]"] = outNameFile;
variables["[extension]"] = util.getExtension(nameFile);
outNameFile = getOutputFileName("name", variables);
outputNames.push_back(outNameFile); outputTypes["name"].push_back(outNameFile);
ofstream outName; util.openOutputFile(outNameFile, outName);
util.readNames(nameFile, nameMap);
for (map<string, vector<string> >::iterator itNames = nameMap.begin(); itNames != nameMap.end(); itNames++) {
vector<string> dups = itNames->second;
if (m->getControl_pressed()) { break; }
if (oldMapEmpty) {
for (int i = 0; i < dups.size(); i++) {
string group = "";
if (groupfile != "") {
group = groupMap->getGroup(dups[i]);
}else if (countfile != "") {
if (hasGroups) {
vector<string> thisReadsGroups = countTable->getGroups(dups[i]);
if (thisReadsGroups.size() == 0) { group = "not found"; }
else if (thisReadsGroups.size() == 1) { group = thisReadsGroups[0]; }
else { group = "Multi"; }
}
}
if (group == "not found") { m->mothurOut("[ERROR]: " + dups[i] + " is not in your file, please correct.\n"); m->setControl_pressed(true); }
else {
string newName = toString(counts[group]); counts[group]++;
if ((placement == "back") && (group != "")) { newName += delim + group; }
else if (group != "") { newName = group + delim + newName; }
if (i == 0) { outName << newName << '\t' << newName; }
else { outName << "," << newName; }
oldMap[newName] = dups[i];
old2NewNameMap[dups[i]] = newName;
}
}
outName << endl;
}else {
for (int i = 0; i < dups.size(); i++) {
//get new name
string newName = "";
map<string, string>::iterator itMap = oldMap.find(dups[i]);
if (itMap == oldMap.end()) { m->mothurOut("[ERROR]: " + dups[i] + " is not in your map file, please correct.\n"); m->setControl_pressed(true);}
else { newName = itMap->second; }
if (i == 0) { outName << newName << '\t' << newName; }
else { outName << "," << newName; }
old2NewNameMap[dups[i]] = newName;
}
outName << endl;
}
}
outName.close();
}
if (groupfile != "") {
thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(groupfile); }
string outGroupFile = thisOutputDir + util.getRootName(util.getSimpleName(groupfile));
map<string, string> variables;
variables["[filename]"] = outGroupFile;
variables["[extension]"] = util.getExtension(groupfile);
outGroupFile = getOutputFileName("group", variables);
outputNames.push_back(outGroupFile); outputTypes["group"].push_back(outGroupFile);
ofstream outGroup; util.openOutputFile(outGroupFile, outGroup);
vector<string> namesOfSeqs = groupMap->getNamesSeqs();
for (int i = 0; i < namesOfSeqs.size(); i++) {
string group = groupMap->getGroup(namesOfSeqs[i]);
string newName = "";
if (group == "not found") { m->mothurOut("[ERROR]: " + namesOfSeqs[i] + " is not in your file, please correct.\n"); m->setControl_pressed(true); }
if (m->getControl_pressed()) { break; }
if (oldMapEmpty) {
map<string, string>::iterator itMap = old2NewNameMap.find(namesOfSeqs[i]);
if (itMap == old2NewNameMap.end()) {
newName = toString(counts[group]); counts[group]++;
if ((placement == "back") && (group != "")) { newName += delim + group; }
else if (group != "") { newName = group + delim + newName; }
}else {
newName = itMap->second; //newName = name from namefile
}
oldMap[newName] = namesOfSeqs[i];
}else {
map<string, string>::iterator itMap = oldMap.find(namesOfSeqs[i]);
if (itMap == oldMap.end()) { m->mothurOut("[ERROR]: " + namesOfSeqs[i] + " is not in your map file, please correct.\n"); m->setControl_pressed(true);}
else { newName = itMap->second; }
}
outGroup << newName << '\t' << group << endl;
old2NewNameMap[namesOfSeqs[i]] = newName;
}
outGroup.close();
}
if (countfile != "") {
thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(countfile); }
string outCountFile = thisOutputDir + util.getRootName(util.getSimpleName(countfile));
map<string, string> variables;
variables["[filename]"] = outCountFile;
variables["[extension]"] = util.getExtension(countfile);
outCountFile = getOutputFileName("count", variables);
outputNames.push_back(outCountFile); outputTypes["count"].push_back(outCountFile);
vector<string> namesOfSeqs = countTable->getNamesOfSeqs();
for (int i = 0; i < namesOfSeqs.size(); i++) {
if (m->getControl_pressed()) { break; }
string newName = "";
if (oldMapEmpty) {
map<string, string>::iterator itMap = old2NewNameMap.find(namesOfSeqs[i]);
if (itMap == old2NewNameMap.end()) {
string group = "";
if (hasGroups) {
vector<string> thisReadsGroups = countTable->getGroups(namesOfSeqs[i]);
if (thisReadsGroups.size() == 0) { group = "not found"; }
else if (thisReadsGroups.size() == 1) { group = thisReadsGroups[0]; }
else { group = "Multi"; }
}
if (group == "not found") { m->mothurOut("[ERROR]: " + namesOfSeqs[i] + " is not in your file, please correct.\n"); m->setControl_pressed(true); }
newName = toString(counts[group]); counts[group]++;
if ((placement == "back") && (group != "")) { newName += delim + group; }
else if (group != "") { newName = group + delim + newName; }
}else { newName = itMap->second; }
oldMap[newName] = namesOfSeqs[i];
}else {
map<string, string>::iterator itMap = oldMap.find(namesOfSeqs[i]);
if (itMap == oldMap.end()) { m->mothurOut("[ERROR]: " + namesOfSeqs[i] + " is not in your map file, please correct.\n"); m->setControl_pressed(true);}
else { newName = itMap->second; }
}
countTable->renameSeq(namesOfSeqs[i], newName);
old2NewNameMap[namesOfSeqs[i]] = newName;
}
countTable->printTable(outCountFile);
}
if (m->getControl_pressed()) { for (int i = 0; i < outputNames.size(); i++) { util.mothurRemove(outputNames[i]); } }
if (groupMap != nullptr) { delete groupMap; }
if (countTable != nullptr) { delete countTable; }
return;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
void RenameSeqsCommand::readFasta(map<string, string>& oldMap){
try {
//prepare filenames and open files
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(fastaFile); }
string outFastaFile = thisOutputDir + util.getRootName(util.getSimpleName(fastaFile));
map<string, string> variables;
variables["[filename]"] = outFastaFile;
variables["[extension]"] = util.getExtension(fastaFile);
outFastaFile = getOutputFileName("fasta", variables);
outputNames.push_back(outFastaFile); outputTypes["fasta"].push_back(outFastaFile);
ifstream in; util.openInputFile(fastaFile, in);
ofstream out; util.openOutputFile(outFastaFile, out);
map<string, string>::iterator it;
int count = 0;
while(!in.eof()){
if (m->getControl_pressed()) { break; }
Sequence seq(in); gobble(in);
it = oldMap.find(seq.getName());
if (it == oldMap.end()) { //not in other files, create name
if (!ignoreNew) {
oldMap[seq.getName()] = toString(count);
seq.setName(toString(count)); count++;
}
}else {
seq.setName(it->second);
}
seq.printSequence(out);
}
in.close(); out.close();
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readFasta");
exit(1);
}
}
//**********************************************************************************************************************
string RenameSeqsCommand::readFastq(map<string, string>& oldMap){
try {
bool oldMapEmpty = true;
if (oldMap.size() != 0) { oldMapEmpty = false; }
//prepare filenames and open files
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(fastqfile); }
string outFastqFile = thisOutputDir + util.getRootName(util.getSimpleName(fastqfile));
map<string, string> variables;
variables["[filename]"] = outFastqFile;
variables["[extension]"] = ".fastq";
outFastqFile = getOutputFileName("fastq", variables);
outputNames.push_back(outFastqFile); outputTypes["fastq"].push_back(outFastqFile);
//open input file
ifstream inFastq;
#ifdef USE_BOOST
boost::iostreams::filtering_istream inFastqBoost;
#endif
if (!gz) { util.openInputFile(fastqfile, inFastq); }
else { //compressed files
#ifdef USE_BOOST
util.openInputFileBinary(fastqfile, inFastq, inFastqBoost);
#endif
}
#ifdef USE_BOOST
#else
if (gz) { m->mothurOut("[ERROR]: Your files are in compressed .gz form and you do not have the boost library install, quitting.\n"); m->setControl_pressed(true); return 0; }
#endif
string format = "illumina1.8+";
ofstream out; util.openOutputFile(outFastqFile, out);
map<string, string>::iterator it;
int count = 0;
bool good = true;
while (good) {
if (m->getControl_pressed()) { break; }
bool tignore = false;
FastqRead* fread;
if (gz) {
#ifdef USE_BOOST
fread = new FastqRead(inFastqBoost, tignore, format); gobble(inFastqBoost);
#endif
}else {
fread = new FastqRead(inFastq, tignore, format); gobble(inFastq);
}
string newName = toString(count);
if (oldMapEmpty) {
if ((placement == "back") && (groupName != "")) { newName += delim + groupName; }
else if (groupName != "") { newName = groupName + delim + newName; }
oldMap[fread->getName()] = newName;
fread->setName(newName);
count++;
}else {
it = oldMap.find(fread->getName());
if (it == oldMap.end()) { //not in other files, create name
if (!ignoreNew) {
oldMap[fread->getName()] = newName; count++;
fread->setName(newName);
}
}else {
fread->setName(it->second);
}
}
fread->printFastq(out);
delete fread;
if (gz) {
#ifdef USE_BOOST
if (inFastqBoost.eof()) { good = false; break; }
#endif
}else {
if (inFastq.eof()) { good = false; break; }
}
}
if (gz) {
#ifdef USE_BOOST
inFastqBoost.pop();
#endif
}else {
inFastq.close();
}
out.close();
return outFastqFile;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readFastq");
exit(1);
}
}
//**********************************************************************************************************************
void RenameSeqsCommand::readQual(map<string, string>& oldMap){
try {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(qualfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(qualfile));
variables["[extension]"] = util.getExtension(qualfile);
string outputFileName = getOutputFileName("qfile", variables);
outputNames.push_back(outputFileName); outputTypes["qfile"].push_back(outputFileName);
ofstream out; util.openOutputFile(outputFileName, out);
ifstream in; util.openInputFile(qualfile, in);
map<string, string>::iterator it;
int count = 0;
while(!in.eof()){
if (m->getControl_pressed()) { break; }
QualityScores qual(in); gobble(in);
it = oldMap.find(qual.getName());
if (it == oldMap.end()) {
if (!ignoreNew) {
oldMap[qual.getName()] = toString(count);
qual.setName(toString(count)); count++;
}
}else {
qual.setName(it->second);
}
qual.printQScores(out);
}
in.close(); out.close();
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readQual");
exit(1);
}
}
//**********************************************************************************************************************
void RenameSeqsCommand::readTax(map<string, string>& oldMap){
try {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(taxfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(taxfile));
variables["[extension]"] = util.getExtension(taxfile);
string outputFileName = getOutputFileName("taxonomy", variables);
outputNames.push_back(outputFileName); outputTypes["taxonomy"].push_back(outputFileName);
ofstream out; util.openOutputFile(outputFileName, out);
ifstream in; util.openInputFile(taxfile, in);
string name, tax; int count = 0;
map<string, string>::iterator it;
while(!in.eof()){
if (m->getControl_pressed()) { break; }
in >> name; gobble(in);
tax = util.getline(in); gobble(in);
it = oldMap.find(name);
if (it == oldMap.end()) {
if (!ignoreNew) {
oldMap[name] = toString(count);
name = toString(count); count++;
}
}else {
name = it->second;
}
out << name << '\t' << tax << endl;
}
in.close(); out.close();
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readTax");
exit(1);
}
}
//**********************************************************************************************************************
void RenameSeqsCommand::readContigs(map<string, string>& oldMap){
try {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(contigsfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(contigsfile));
variables["[extension]"] = util.getExtension(contigsfile);
string outputFileName = getOutputFileName("contigsreport", variables);
ofstream out;
util.openOutputFile(outputFileName, out);
outputNames.push_back(outputFileName); outputTypes["contigsreport"].push_back(outputFileName);
ifstream in; util.openInputFile(contigsfile, in);
ContigsReport report;
report.readHeaders(in); gobble(in);
report.printHeaders(out);
map<string, string>::iterator it;
int count = 0;
while (!in.eof()) {
if (m->getControl_pressed()) { break; }
report.read(in); gobble(in);
it = oldMap.find(report.getName());
if (it != oldMap.end()) { report.setName(it->second); }
else {
if (!ignoreNew) {
oldMap[report.getName()] = toString(count);
report.setName(toString(count)); count++;
}
}
report.print(out);
}
in.close(); out.close();
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readContigs");
exit(1);
}
}
//**********************************************************************************************************************
void RenameSeqsCommand::readList(map<string, string>& oldMap){
try {
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(listfile); }
map<string, string> variables;
variables["[filename]"] = thisOutputDir + util.getRootName(util.getSimpleName(listfile));
variables["[extension]"] = util.getExtension(listfile);
string outputFileName = getOutputFileName("list", variables);
ofstream out; util.openOutputFile(outputFileName, out);
outputNames.push_back(outputFileName); outputTypes["list"].push_back(outputFileName);
InputData input(listfile, "list", nullVector);
set<string> processedLabels;
set<string> userLabels;
string lastLabel = "";
bool printHeaders = true;
ListVector* list = util.getNextList(input, true, userLabels, processedLabels, lastLabel);
while (list != nullptr) {
if (m->getControl_pressed()) { delete list; break; }
list->setPrintedLabels(printHeaders);
//process list
int count = 0;
for (int i = 0; i < list->getNumBins(); i++) {
string bin = list->get(i);
vector<string> names; util.splitAtComma(bin, names);
for (int j = 0; j < names.size(); j++) {
map<string, string>::iterator it = oldMap.find(names[j]);
if (it == oldMap.end()) {
if (!ignoreNew) {
string newName = toString(count); count++;
oldMap[names[j]] = newName;
names[j] = newName;
}
}else {
names[j] = it->second;
}
}
bin = util.getStringFromVector(names, ",");
list->set(i, bin);
}
//print list
list->print(out); printHeaders = false;
delete list;
list = util.getNextList(input, true, userLabels, processedLabels, lastLabel);
}
out.close();
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readList");
exit(1);
}
}
//**********************************************************************************************************************
int RenameSeqsCommand::readMapFile(map<string, string>& readMap){
try {
ifstream in; util.openInputFile(mapFile, in);
map<string, string>::iterator it;
string oldname, newname;
while (!in.eof()) {
if (m->getControl_pressed()) { break; }
in >> oldname; gobble(in);
in >> newname; gobble(in);
it = readMap.find(oldname);
if (it != readMap.end()) {
m->mothurOut("[ERROR]: " + oldname + " is already in your map file. Sequence names must be unique, quitting.\n"); m->setControl_pressed(true);
}else {
readMap[oldname] = newname;
}
}
in.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readMapFile");
exit(1);
}
}
//**********************************************************************************************************************
int RenameSeqsCommand::processFile(){
try {
ignoreNew = true; //if there are sequences only present in some files, ignore them
vector<string> file2Group; gz = false;
vector< vector<string> > files = readFiles(file2Group, gz);
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(fileFile); }
string outFileFile = thisOutputDir + util.getRootName(util.getSimpleName(fileFile));
map<string, string> variables;
variables["[filename]"] = outFileFile;
variables["[extension]"] = util.getExtension(fileFile);
outFileFile = getOutputFileName("file", variables);
outputNames.push_back(outFileFile); outputTypes["file"].push_back(outFileFile);
ofstream outFile; util.openOutputFile(outFileFile, outFile);
for (int i = 0; i < files.size(); i++) {
if (m->getControl_pressed()) { break; }
m->mothurOut("\n>>>>>\tRenaming file pair " + files[i][0] + " - " + files[i][1] + " (files " + toString(i+1) + " of " + toString(files.size()) + ")\t<<<<<\n");
map<string, string> old2NewNameMap; //each file pair gets a map file
string fileOutput = "";
groupName = file2Group[i]; //blank if no group is in file
if (groupName != "") { fileOutput += groupName + '\t'; }
fastqfile = files[i][0]; //forwardFastq
string renamedFile = readFastq(old2NewNameMap);
fileOutput += util.getSimpleName(renamedFile) + '\t';
fastqfile = files[i][1]; //reverseFastq
renamedFile = readFastq(old2NewNameMap);
fileOutput += util.getSimpleName(renamedFile) + '\t';
if (files[i][2] != "") { //blank if no forward index is in file
fastqfile = files[i][2]; //forwardIndex
renamedFile = readFastq(old2NewNameMap);
fileOutput += util.getSimpleName(renamedFile) + '\t';
}
if (files[i][3] != "") { //blank if no reverse index is in file
fastqfile = files[i][3]; //reverseIndex
renamedFile = readFastq(old2NewNameMap);
fileOutput += util.getSimpleName(renamedFile) + '\t';
}
string thisOutputDir = outputdir;
if (outputdir == "") { thisOutputDir += util.hasPath(files[i][0]); }
string outMapFile = thisOutputDir + util.getRootName(util.getSimpleName(files[i][0]));
map<string, string> variables;
variables["[filename]"] = outMapFile;
outMapFile = getOutputFileName("map", variables);
outputNames.push_back(outMapFile); outputTypes["map"].push_back(outMapFile);
ofstream outMap; util.openOutputFile(outMapFile, outMap);
//print map
for(map<string, string>::iterator it = old2NewNameMap.begin(); it != old2NewNameMap.end(); it++) {
outMap << it->second << '\t' << it->first << endl;
}
outMap.close();
//print renamed filenames to new file file
outFile << fileOutput << endl;
}
outFile.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "processFile");
exit(1);
}
}
//**********************************************************************************************************************
vector< vector<string> > RenameSeqsCommand::readFiles(vector<string>& file2Group, bool& isGZ){
try {
FileFile dataFile(fileFile, "contigs");
vector< vector<string> > dataFiles = dataFile.getFiles();
int dataFileFormat = dataFile.getFileFormat();
file2Group = dataFile.getGroupNames();
isGZ = dataFile.isGZ();
if (file2Group.size() == 0) { m->setControl_pressed(true); }
return dataFiles;
}
catch(exception& e) {
m->errorOut(e, "RenameSeqsCommand", "readFiles");
exit(1);
}
}
/**************************************************************************************/
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