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/*
* summaryqualcommand.cpp
* Mothur
*
* Created by westcott on 11/28/11.
* Copyright 2011 Schloss Lab. All rights reserved.
*
*/
#include "summaryqualcommand.h"
#include "counttable.h"
//**********************************************************************************************************************
vector<string> SummaryQualCommand::setParameters(){
try {
CommandParameter pqual("qfile", "InputTypes", "", "", "none", "none", "none","summary",false,true,true); parameters.push_back(pqual);
CommandParameter pname("name", "InputTypes", "", "", "namecount", "none", "none","",false,false,true); parameters.push_back(pname);
CommandParameter pcount("count", "InputTypes", "", "", "namecount", "none", "none","",false,false,true); parameters.push_back(pcount);
CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false,true); parameters.push_back(pprocessors);
CommandParameter pseed("seed", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pseed);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
vector<string> tempOutNames;
outputTypes["summary"] = tempOutNames;
abort = false; calledHelp = false;
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string SummaryQualCommand::getHelpString(){
try {
string helpString = "";
helpString += "The summary.qual command reads a quality file and an optional name or count file, and summarizes the quality information.\n";
helpString += "The summary.qual command parameters are qfile, name, count and processors. qfile is required, unless you have a valid current quality file.\n";
helpString += "The name parameter allows you to enter a name file associated with your quality file. \n";
helpString += "The count parameter allows you to enter a count file associated with your quality file. \n";
helpString += "The summary.qual command should be in the following format: \n";
helpString += "summary.qual(qfile=yourQualityFile) \n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string SummaryQualCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "summary") { pattern = "[filename],qual.summary"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->setControl_pressed(true); }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "getOutputPattern");
exit(1);
}
}
//***************************************************************************************************************
SummaryQualCommand::SummaryQualCommand(string option) : Command() {
try {
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else if(option == "category") { abort = true; calledHelp = true; }
else {
OptionParser parser(option, setParameters());
map<string,string> parameters = parser.getParameters();
ValidParameters validParameter;
qualfile = validParameter.validFile(parameters, "qfile");
if (qualfile == "not open") { qualfile = ""; abort = true; }
else if (qualfile == "not found") {
qualfile = current->getQualFile();
if (qualfile != "") { m->mothurOut("Using " + qualfile + " as input file for the qfile parameter.\n"); }
else { m->mothurOut("You have no current quality file and the qfile parameter is required.\n"); abort = true; }
}else { current->setQualFile(qualfile); }
namefile = validParameter.validFile(parameters, "name");
if (namefile == "not open") { namefile = ""; abort = true; }
else if (namefile == "not found") { namefile = ""; }
else { current->setNameFile(namefile); }
countfile = validParameter.validFile(parameters, "count");
if (countfile == "not open") { abort = true; countfile = ""; }
else if (countfile == "not found") { countfile = ""; }
else { current->setCountFile(countfile); }
if ((countfile != "") && (namefile != "")) { m->mothurOut("You must enter ONLY ONE of the following: count or name.\n"); abort = true; }
if (outputdir == ""){ outputdir += util.hasPath(qualfile); }
string temp = validParameter.valid(parameters, "processors"); if (temp == "not found"){ temp = current->getProcessors(); }
processors = current->setProcessors(temp);
}
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "SummaryQualCommand");
exit(1);
}
}
//***************************************************************************************************************
int SummaryQualCommand::execute(){
try{
if (abort) { if (calledHelp) { return 0; } return 2; }
long start = time(nullptr);
long long numSeqs = 0;
hasNameMap = false;
vector<int> position;
vector<int> averageQ;
vector< vector<int> > scores;
if (m->getControl_pressed()) { return 0; }
if (namefile != "") { hasNameMap = true; nameMap = util.readNames(namefile); }
else if (countfile != "") {
CountTable ct;
ct.readTable(countfile, false, false);
nameMap = ct.getNameMap();
hasNameMap = true;
}
numSeqs = createProcessesCreateSummary(position, averageQ, scores, qualfile);
if (m->getControl_pressed()) { return 0; }
//print summary file
map<string, string> variables;
variables["[filename]"] = outputdir + util.getRootName(util.getSimpleName(qualfile));
string summaryFile = getOutputFileName("summary",variables);
printQual(summaryFile, position, averageQ, scores);
if (m->getControl_pressed()) { util.mothurRemove(summaryFile); return 0; }
//output results to screen
cout.setf(ios::fixed, ios::floatfield); cout.setf(ios::showpoint);
m->mothurOut("\nPosition\tNumSeqs\tAverageQ\n");
for (int i = 0; i < position.size(); i+=100) {
float average = averageQ[i] / (float) position[i];
cout << i << '\t' << position[i] << '\t' << average << '\n';
m->mothurOutJustToLog(toString(i) + "\t" + toString(position[i]) + "\t" + toString(average)+"\n");
}
outputNames.push_back(summaryFile); outputTypes["summary"].push_back(summaryFile);
m->mothurOut("\nIt took " + toString(time(nullptr) - start) + " secs to create the summary file for " + toString(numSeqs) + " sequences.\n\n");
m->mothurOut("Output File Names: \n");
m->mothurOut(summaryFile+"\n\n");
return 0;
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "execute");
exit(1);
}
}
/**************************************************************************************************/
//custom data structure for threads to use.
// This is passed by void pointer so it can be any data type
// that can be passed using a single void pointer (LPVOID).
struct seqSumQualData {
vector<int> position;
vector<int> averageQ;
vector< vector<int> > scores;
string filename;
unsigned long long start;
unsigned long long end;
int count, numSeqs;
MothurOut* m;
bool hasNameMap;
map<string, int> nameMap;
Utils util;
~seqSumQualData(){}
seqSumQualData(string f, unsigned long long st, unsigned long long en, bool n, map<string, int> nam) {
filename = f;
m = MothurOut::getInstance();
start = st;
end = en;
hasNameMap = n;
nameMap = nam;
count = 0;
}
};
/**************************************************************************************/
void driverCreateSummary(seqSumQualData* params) {
try {
ifstream in; params->util.openInputFile(params->filename, in);
in.seekg(params->start);
//adjust start if null strings
if (params->start == 0) { params->util.zapGremlins(in); gobble(in); }
bool done = false;
params->count = 0;
int count = 0;
while (!done) {
if (params->m->getControl_pressed()) { in.close(); break; }
QualityScores current(in); gobble(in);
if (current.getName() != "") {
int num = 1;
if (params->hasNameMap) {
//make sure this sequence is in the namefile, else error
map<string, int>::iterator it = params->nameMap.find(current.getName());
if (it == params->nameMap.end()) { params->m->mothurOut("[ERROR]: " + current.getName() + " is not in your namefile, please correct.\n"); params->m->setControl_pressed(true); }
else { num = it->second; }
}
vector<int> thisScores = current.getScores();
//resize to num of positions setting number of seqs with that size to 1
if (params->position.size() < thisScores.size()) { params->position.resize(thisScores.size(), 0); }
if (params->averageQ.size() < thisScores.size()) { params->averageQ.resize(thisScores.size(), 0); }
if (params->scores.size() < thisScores.size()) {
params->scores.resize(thisScores.size());
for (int i = 0; i < params->scores.size(); i++) { params->scores[i].resize(41, 0); }
}
//increase counts of number of seqs with this position
//average is really the total, we will average in execute
for (int i = 0; i < thisScores.size(); i++) {
params->position[i] += num;
params->averageQ[i] += (thisScores[i] * num); //weighting for namesfile
if (thisScores[i] > 41) { params->m->mothurOut("[WARNING]: " + current.getName() + " has a quality scores of " + toString(thisScores[i]) + ", expecting values to be less than 40. Setting to 40.\n"); thisScores[i] = 40;
}
else { params->scores[i][thisScores[i]] += num; }
}
params->count += num; //totalSeqs
count++; //uniqueSeqs
}
#if defined NON_WINDOWS
unsigned long long pos = in.tellg();
if ((pos == -1) || (pos >= params->end)) { break; }
#else
if ((count == params->end) || (in.eof())) { break; }
#endif
}
in.close();
}
catch(exception& e) {
params->m->errorOut(e, "SummaryQualCommand", "driverCreateSummary");
exit(1);
}
}
/**************************************************************************************************/
long long SummaryQualCommand::createProcessesCreateSummary(vector<int>& position, vector<int>& averageQ, vector< vector<int> >& scores, string filename) {
try {
long long numSeqs = 0;
vector<double> positions;
vector<linePair> lines;
#if defined NON_WINDOWS
positions = util.divideFile(filename, processors);
for (int i = 0; i < (positions.size()-1); i++) { lines.push_back(linePair(positions[i], positions[(i+1)])); }
#else
positions = util.setFilePosFasta(qualfile, numSeqs);
if (numSeqs < processors) { processors = numSeqs; }
//figure out how many sequences you have to process
int numSeqsPerProcessor = numSeqs / processors;
for (int i = 0; i < processors; i++) {
int startIndex = i * numSeqsPerProcessor;
if(i == (processors - 1)){ numSeqsPerProcessor = numSeqs - i * numSeqsPerProcessor; }
lines.push_back(linePair(positions[startIndex], numSeqsPerProcessor));
}
#endif
//create array of worker threads
vector<std::thread*> workerThreads;
vector<seqSumQualData*> data;
//string f, unsigned long long st, unsigned long long en, bool n, map<string, int> nam
//Lauch worker threads
for (int i = 0; i < processors-1; i++) {
seqSumQualData* dataBundle = new seqSumQualData(filename, lines[i+1].start, lines[i+1].end, hasNameMap, nameMap);
data.push_back(dataBundle);
workerThreads.push_back(new std::thread(driverCreateSummary, dataBundle));
}
seqSumQualData* dataBundle = new seqSumQualData(filename, lines[0].start, lines[0].end, hasNameMap, nameMap);
driverCreateSummary(dataBundle);
numSeqs = dataBundle->count;
position = dataBundle->position;
averageQ = dataBundle->averageQ;
scores = dataBundle->scores;
for (int i = 0; i < processors-1; i++) {
workerThreads[i]->join();
numSeqs += data[i]->count;
int tempNum = data[i]->position.size();
if (position.size() < tempNum) { position.resize(tempNum, 0); }
if (averageQ.size() < tempNum) { averageQ.resize(tempNum, 0); }
if (scores.size() < tempNum) {
scores.resize(tempNum);
for (int i = 0; i < scores.size(); i++) { scores[i].resize(41, 0); }
}
for (int k = 0; k < tempNum; k++) { position[k] += data[i]->position[k]; }
for (int k = 0; k < tempNum; k++) { averageQ[k] += data[i]->averageQ[k]; }
for (int k = 0; k < tempNum; k++) { for (int j = 0; j < 41; j++) { scores[k][j] += data[i]->scores[k][j]; } }
delete data[i];
delete workerThreads[i];
}
delete dataBundle;
return numSeqs;
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "createProcessesCreateSummary");
exit(1);
}
}
/**************************************************************************************************/
int SummaryQualCommand::printQual(string sumFile, vector<int>& position, vector<int>& averageQ, vector< vector<int> >& scores) {
try {
ofstream out;
util.openOutputFile(sumFile, out);
out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
outputNames.push_back(sumFile); outputTypes["summary"].push_back(sumFile);
//print headings
out << "Position\tnumSeqs\tAverageQ";
for (int i = 0; i < 41; i++) { out << '\t' << "q" << i; }
out << endl;
for (int i = 0; i < position.size(); i++) {
if (m->getControl_pressed()) { out.close(); return 0; }
double average = averageQ[i] / (float) position[i];
out << i << '\t' << position[i] << '\t' << average;
for (int j = 0; j < 41; j++) {
out << '\t' << scores[i][j];
}
out << endl;
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "SummaryQualCommand", "printQual");
exit(1);
}
}
/**************************************************************************************/
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