PRIMER_MAX_SELF_ANY=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_END=8
P3_FILE_FLAG=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_TASK=pick_pcr_primers
SEQUENCE_ID=pcr_primers
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
SEQUENCE_TARGET=21,122
PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 3, ok 3
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=3.432274
PRIMER_LEFT_0_PENALTY=1.117633
PRIMER_RIGHT_0_PENALTY=2.314641
PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_0=0,21
PRIMER_RIGHT_0=160,18
PRIMER_LEFT_0_TM=60.118
PRIMER_RIGHT_0_TM=59.685
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=12.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=12.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=161
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=4.086374
PRIMER_LEFT_1_PENALTY=1.771733
PRIMER_RIGHT_1_PENALTY=2.314641
PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=160,18
PRIMER_LEFT_1_TM=58.228
PRIMER_RIGHT_1_TM=59.685
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=160
PRIMER_PAIR_1_PRODUCT_TM=74.3
PRIMER_PAIR_2_PENALTY=5.046339
PRIMER_LEFT_2_PENALTY=2.731698
PRIMER_RIGHT_2_PENALTY=2.314641
PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=160,18
PRIMER_LEFT_2_TM=57.268
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=161
PRIMER_PAIR_2_PRODUCT_TM=74.3
=
SEQUENCE_ID=pcr_primers_and_io
PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 30, high tm 29, ok 12
PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 7, high tm 44, ok 23
PRIMER_INTERNAL_EXPLAIN=considered 393, too many Ns 280, low tm 30, high tm 46, long poly-x seq 3, ok 34
PRIMER_PAIR_EXPLAIN=considered 5, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=2.334195
PRIMER_LEFT_0_PENALTY=2.101896
PRIMER_RIGHT_0_PENALTY=0.232299
PRIMER_INTERNAL_0_PENALTY=4.798289
PRIMER_LEFT_0_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_0_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_0_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_0=9,22
PRIMER_RIGHT_0=247,20
PRIMER_INTERNAL_0=141,18
PRIMER_LEFT_0_TM=59.898
PRIMER_RIGHT_0_TM=60.232
PRIMER_INTERNAL_0_TM=62.798
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=4.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=239
PRIMER_PAIR_0_PRODUCT_TM=75.7
PRIMER_PAIR_1_PENALTY=2.773493
PRIMER_LEFT_1_PENALTY=2.101896
PRIMER_RIGHT_1_PENALTY=0.671597
PRIMER_INTERNAL_1_PENALTY=4.798289
PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
PRIMER_INTERNAL_1_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=246,20
PRIMER_INTERNAL_1=141,18
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.328
PRIMER_INTERNAL_1_TM=62.798
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=66.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=238
PRIMER_PAIR_1_PRODUCT_TM=75.8
PRIMER_PAIR_2_PENALTY=2.846821
PRIMER_LEFT_2_PENALTY=2.101896
PRIMER_RIGHT_2_PENALTY=0.744925
PRIMER_INTERNAL_2_PENALTY=4.798289
PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
PRIMER_INTERNAL_2_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=248,20
PRIMER_INTERNAL_2=141,18
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.745
PRIMER_INTERNAL_2_TM=62.798
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_2_GC_PERCENT=66.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=240
PRIMER_PAIR_2_PRODUCT_TM=75.7
PRIMER_PAIR_3_PENALTY=3.098430
PRIMER_LEFT_3_PENALTY=2.866131
PRIMER_RIGHT_3_PENALTY=0.232299
PRIMER_INTERNAL_3_PENALTY=4.798289
PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_3_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_3=11,21
PRIMER_RIGHT_3=247,20
PRIMER_INTERNAL_3=141,18
PRIMER_LEFT_3_TM=58.134
PRIMER_RIGHT_3_TM=60.232
PRIMER_INTERNAL_3_TM=62.798
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=237
PRIMER_PAIR_3_PRODUCT_TM=75.7
PRIMER_PAIR_4_PENALTY=3.235559
PRIMER_LEFT_4_PENALTY=3.003259
PRIMER_RIGHT_4_PENALTY=0.232299
PRIMER_INTERNAL_4_PENALTY=4.798289
PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_4_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=247,20
PRIMER_INTERNAL_4=141,18
PRIMER_LEFT_4_TM=58.997
PRIMER_RIGHT_4_TM=60.232
PRIMER_INTERNAL_4_TM=62.798
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=238
PRIMER_PAIR_4_PRODUCT_TM=75.8
=
SEQUENCE_ID=left_only
PRIMER_TASK=pick_left_only
SEQUENCE_TARGET=100,20
SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 42, high tm 866, ok 152
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.040772
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=72,20
PRIMER_LEFT_0_TM=59.959
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_LEFT_1_PENALTY=0.271680
PRIMER_LEFT_1_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_LEFT_1=64,20
PRIMER_LEFT_1_TM=59.728
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_LEFT_2_PENALTY=0.271680
PRIMER_LEFT_2_SEQUENCE=CACCCTGCAGGTTTAGAAGG
PRIMER_LEFT_2=60,20
PRIMER_LEFT_2_TM=59.728
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_LEFT_3_PENALTY=0.312496
PRIMER_LEFT_3_SEQUENCE=AGCACACCCTGCAGGTTTAG
PRIMER_LEFT_3=56,20
PRIMER_LEFT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_LEFT_4_PENALTY=0.407941
PRIMER_LEFT_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_LEFT_4=65,20
PRIMER_LEFT_4_TM=59.592
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
=
SEQUENCE_ID=right_only
PRIMER_TASK=PICK_right_only
SEQUENCE_TARGET=90,10
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 230, high tm 145, high end compl 6, ok 174
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.291669
PRIMER_RIGHT_0_SEQUENCE=GTGCTTTGCGTTTTGGAAGT
PRIMER_RIGHT_0=125,20
PRIMER_RIGHT_0_TM=60.292
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_RIGHT_1_PENALTY=0.727969
PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTTCTATCAGGTCA
PRIMER_RIGHT_1=75,20
PRIMER_RIGHT_1_TM=59.272
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_RIGHT_2_PENALTY=0.782051
PRIMER_RIGHT_2_SEQUENCE=TGCTTTGCGTTTTGGAAGTT
PRIMER_RIGHT_2=124,20
PRIMER_RIGHT_2_TM=60.782
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_RIGHT_3_PENALTY=1.125062
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_RIGHT_3=76,21
PRIMER_RIGHT_3_TM=60.125
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_RIGHT_4_PENALTY=1.255675
PRIMER_RIGHT_4_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_RIGHT_4=79,21
PRIMER_RIGHT_4_TM=60.256
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_RIGHT_4_END_STABILITY=8.2000
=
SEQUENCE_ID=hyb_probe_only
PRIMER_TASK=pick_HYB_probe_ONly
SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20
SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_INTERNAL_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 44, high tm 804, ok 132
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.271680
PRIMER_INTERNAL_0_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_INTERNAL_0=64,20
PRIMER_INTERNAL_0_TM=59.728
PRIMER_INTERNAL_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_INTERNAL_1_PENALTY=0.271680
PRIMER_INTERNAL_1_SEQUENCE=CACCCTGCAGGTTTAGAAGG
PRIMER_INTERNAL_1=60,20
PRIMER_INTERNAL_1_TM=59.728
PRIMER_INTERNAL_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_INTERNAL_2_PENALTY=0.312496
PRIMER_INTERNAL_2_SEQUENCE=AGCACACCCTGCAGGTTTAG
PRIMER_INTERNAL_2=56,20
PRIMER_INTERNAL_2_TM=60.312
PRIMER_INTERNAL_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_SELF_ANY=8.00
PRIMER_INTERNAL_2_SELF_END=6.00
PRIMER_INTERNAL_3_PENALTY=0.375090
PRIMER_INTERNAL_3_SEQUENCE=CATCTGCACCCTGTAAACCC
PRIMER_INTERNAL_3=1,20
PRIMER_INTERNAL_3_TM=60.375
PRIMER_INTERNAL_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_INTERNAL_3_SELF_END=0.00
PRIMER_INTERNAL_4_PENALTY=0.407941
PRIMER_INTERNAL_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_INTERNAL_4=65,20
PRIMER_INTERNAL_4_TM=59.592
PRIMER_INTERNAL_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_INTERNAL_4_SELF_END=0.00
=
SEQUENCE_ID=left_only
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.040772
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.959
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
=
SEQUENCE_ID=right_only
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.040772
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.959
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
=
SEQUENCE_ID=hyb_probe_only
PRIMER_INTERNAL_MIN_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.040772
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.959
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
=