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PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=primer3_config/
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_DIVALENT=1.5
PRIMER_MAX_SELF_ANY=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_END=8
P3_FILE_FLAG=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_TASK=pick_pcr_primers
PRIMER_DNTP_CONC=0.0
SEQUENCE_ID=pcr_primers
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
SEQUENCE_TARGET=21,122
=
SEQUENCE_ID=pcr_primers_and_io
PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
=
SEQUENCE_ID=left_only
PRIMER_TASK=pick_left_only
SEQUENCE_TARGET=100,20
SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
=
SEQUENCE_ID=right_only
PRIMER_TASK=PICK_right_only
SEQUENCE_TARGET=90,10
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
=
SEQUENCE_ID=hyb_probe_only
PRIMER_TASK=pick_HYB_probe_ONly
SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20
SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
=
SEQUENCE_ID=left_only
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
=
SEQUENCE_ID=right_only
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
=
SEQUENCE_ID=hyb_probe_only
PRIMER_INTERNAL_MIN_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
=
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