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# Copyright 2010-2013 by Peter Cock.
# All rights reserved.
# This code is part of the Biopython distribution and governed by its
# license. Please see the LICENSE file that should have been included
# as part of this package.
"""Test code for working with BGZF files (used in BAM files).
See also the doctests in bgzf.py which are called via run_tests.py
"""
import unittest
import gzip
import os
from random import shuffle
from Bio._py3k import _as_bytes, _as_string
_empty_bytes_string = _as_bytes("")
from Bio import bgzf
def _have_bug17666():
"""Debug function to check if Python's gzip is broken (PRIVATE).
Checks for http://bugs.python.org/issue17666 expected in Python 2.7.4,
3.2.4 and 3.3.1 only.
"""
from io import BytesIO
h = gzip.GzipFile(fileobj=BytesIO(bgzf._bgzf_eof))
try:
data = h.read()
h.close()
assert not data, "Should be zero length, not %i" % len(data)
return False
except TypeError as err:
# TypeError: integer argument expected, got 'tuple'
return True
if _have_bug17666():
from Bio import MissingPythonDependencyError
raise MissingPythonDependencyError("Your Python has a broken gzip library, see "
"http://bugs.python.org/issue17666 for details")
class BgzfTests(unittest.TestCase):
def setUp(self):
self.temp_file = "temp.bgzf"
if os.path.isfile(self.temp_file):
os.remove(self.temp_file)
def tearDown(self):
if os.path.isfile(self.temp_file):
os.remove(self.temp_file)
def rewrite(self, compressed_input_file, output_file):
h = gzip.open(compressed_input_file, "rb")
data = h.read()
h.close()
with bgzf.BgzfWriter(output_file, "wb") as h:
h.write(data)
self.assertFalse(h.seekable())
self.assertFalse(h.isatty())
self.assertEqual(h.fileno(), h._handle.fileno())
# Context manager should call close(),
# Gives empty BGZF block as BAM EOF marker
h = gzip.open(output_file)
new_data = h.read()
h.close()
# Check the decompressed files agree
self.assertTrue(new_data, "Empty BGZF file?")
self.assertEqual(len(data), len(new_data))
self.assertEqual(data, new_data)
def check_blocks(self, old_file, new_file):
h = open(old_file, "rb")
old = list(bgzf.BgzfBlocks(h))
h.close()
h = open(new_file, "rb")
new = list(bgzf.BgzfBlocks(h))
h.close()
self.assertEqual(len(old), len(new))
self.assertEqual(old, new)
def check_text(self, old_file, new_file):
"""Check text mode using explicit open/close"""
h = open(old_file) # text mode!
old_line = h.readline()
old = old_line + h.read()
h.close()
h = bgzf.BgzfReader(new_file, "r") # Text mode!
new_line = h.readline()
new = new_line + h.read(len(old))
h.close()
self.assertEqual(old_line, new_line)
self.assertEqual(len(old), len(new))
self.assertEqual(old, new)
def check_text_with(self, old_file, new_file):
"""Check text mode using context manager (with statement)"""
with open(old_file) as h: # text mode!
old_line = h.readline()
old = old_line + h.read()
with bgzf.BgzfReader(new_file, "r") as h: # Text mode!
new_line = h.readline()
new = new_line + h.read(len(old))
self.assertEqual(old_line, new_line)
self.assertEqual(len(old), len(new))
self.assertEqual(old, new)
def check_by_line(self, old_file, new_file, old_gzip=False):
for mode in ["r", "rb"]:
if old_gzip:
h = gzip.open(old_file, mode)
else:
h = open(old_file, mode)
old = h.read()
# Seems gzip can return bytes even if mode="r",
# perhaps a bug in Python 3.2?
if "b" in mode:
old = _as_bytes(old)
else:
old = _as_string(old)
h.close()
for cache in [1, 10]:
h = bgzf.BgzfReader(new_file, mode, max_cache=cache)
if "b" in mode:
new = _empty_bytes_string.join(line for line in h)
else:
new = "".join(line for line in h)
h.close()
self.assertEqual(len(old), len(new))
self.assertEqual(old[:10], new[:10],
"%r vs %r, mode %r" % (old[:10], new[:10], mode))
self.assertEqual(old, new)
def check_by_char(self, old_file, new_file, old_gzip=False):
for mode in ["r", "rb"]:
if old_gzip:
h = gzip.open(old_file, mode)
else:
h = open(old_file, mode)
old = h.read()
# Seems gzip can return bytes even if mode="r",
# perhaps a bug in Python 3.2?
if "b" in mode:
old = _as_bytes(old)
else:
old = _as_string(old)
h.close()
for cache in [1, 10]:
h = bgzf.BgzfReader(new_file, mode, max_cache=cache)
temp = []
while True:
char = h.read(1)
if not char:
break
temp.append(char)
if "b" in mode:
new = _empty_bytes_string.join(temp)
else:
new = "".join(temp)
del temp
h.close()
self.assertEqual(len(old), len(new))
# If bytes vs unicode mismatch, give a short error message:
self.assertEqual(old[:10], new[:10],
"%r vs %r, mode %r" % (old[:10], new[:10], mode))
self.assertEqual(old, new)
def check_random(self, filename):
"""Check BGZF random access by reading blocks in forward & reverse order"""
h = gzip.open(filename, "rb")
old = h.read()
h.close()
h = open(filename, "rb")
blocks = list(bgzf.BgzfBlocks(h))
h.close()
# Forward, using explicit open/close
new = _empty_bytes_string
h = bgzf.BgzfReader(filename, "rb")
self.assertTrue(h.seekable())
self.assertFalse(h.isatty())
self.assertEqual(h.fileno(), h._handle.fileno())
for start, raw_len, data_start, data_len in blocks:
h.seek(bgzf.make_virtual_offset(start, 0))
data = h.read(data_len)
self.assertEqual(len(data), data_len)
# self.assertEqual(start + raw_len, h._handle.tell())
self.assertEqual(len(new), data_start)
new += data
h.close()
self.assertEqual(len(old), len(new))
self.assertEqual(old, new)
# Reverse, using with statement
new = _empty_bytes_string
with bgzf.BgzfReader(filename, "rb") as h:
for start, raw_len, data_start, data_len in blocks[::-1]:
h.seek(bgzf.make_virtual_offset(start, 0))
data = h.read(data_len)
self.assertEqual(len(data), data_len)
# self.assertEqual(start + raw_len, h._handle.tell())
new = data + new
self.assertEqual(len(old), len(new))
self.assertEqual(old, new)
# Jump back - non-sequential seeking
if len(blocks) >= 3:
h = bgzf.BgzfReader(filename, "rb", max_cache=1)
# Seek to a late block in the file,
# half way into the third last block
start, raw_len, data_start, data_len = blocks[-3]
voffset = bgzf.make_virtual_offset(start, data_len // 2)
h.seek(voffset)
self.assertEqual(voffset, h.tell())
data = h.read(1000)
self.assertTrue(data in old)
self.assertEqual(old.find(data), data_start + data_len // 2)
# Now seek to an early block in the file,
# half way into the second block
start, raw_len, data_start, data_len = blocks[1]
h.seek(bgzf.make_virtual_offset(start, data_len // 2))
voffset = bgzf.make_virtual_offset(start, data_len // 2)
h.seek(voffset)
self.assertEqual(voffset, h.tell())
# Now read all rest of this block and start of next block
data = h.read(data_len + 1000)
self.assertTrue(data in old)
self.assertEqual(old.find(data), data_start + data_len // 2)
h.close()
# Check seek/tell at block boundaries
v_offsets = []
for start, raw_len, data_start, data_len in blocks:
for within_offset in [0, 1, data_len // 2, data_len - 1]:
if within_offset < 0 or data_len <= within_offset:
continue
voffset = bgzf.make_virtual_offset(start, within_offset)
real_offset = data_start + within_offset
v_offsets.append((voffset, real_offset))
shuffle(v_offsets)
h = bgzf.BgzfReader(filename, "rb", max_cache=1)
for voffset, real_offset in v_offsets:
h.seek(0)
self.assertTrue(voffset >= 0 and real_offset >= 0)
self.assertEqual(h.read(real_offset), old[:real_offset])
self.assertEqual(h.tell(), voffset)
for voffset, real_offset in v_offsets:
h.seek(voffset)
self.assertEqual(h.tell(), voffset)
h.close()
def test_random_bam_ex1(self):
"""Check random access to SamBam/ex1.bam"""
self.check_random("SamBam/ex1.bam")
def test_random_bam_ex1_refresh(self):
"""Check random access to SamBam/ex1_refresh.bam"""
self.check_random("SamBam/ex1_refresh.bam")
def test_random_bam_ex1_header(self):
"""Check random access to SamBam/ex1_header.bam"""
self.check_random("SamBam/ex1_header.bam")
def test_random_wnts_xml(self):
"""Check random access to Blast/wnts.xml.bgz"""
self.check_random("Blast/wnts.xml.bgz")
def test_random_example_fastq(self):
"""Check random access to Quality/example.fastq.bgz (Unix newlines)"""
self.check_random("Quality/example.fastq.bgz")
def test_random_example_dos_fastq(self):
"""Check random access to Quality/example_dos.fastq.bgz (DOS newlines)"""
self.check_random("Quality/example_dos.fastq.bgz")
def test_random_example_cor6(self):
"""Check random access to GenBank/cor6_6.gb.bgz"""
self.check_random("GenBank/cor6_6.gb.bgz")
def test_text_wnts_xml(self):
"""Check text mode access to Blast/wnts.xml.bgz"""
self.check_text("Blast/wnts.xml", "Blast/wnts.xml.bgz")
self.check_text_with("Blast/wnts.xml", "Blast/wnts.xml.bgz")
def test_text_example_fastq(self):
"""Check text mode access to Quality/example.fastq.bgz"""
self.check_text("Quality/example.fastq", "Quality/example.fastq.bgz")
self.check_text_with("Quality/example.fastq", "Quality/example.fastq.bgz")
def test_iter_wnts_xml(self):
"""Check iteration over Blast/wnts.xml.bgz"""
self.check_by_line("Blast/wnts.xml", "Blast/wnts.xml.bgz")
self.check_by_char("Blast/wnts.xml", "Blast/wnts.xml.bgz")
def test_iter_example_fastq(self):
"""Check iteration over Quality/example.fastq.bgz"""
self.check_by_line("Quality/example.fastq", "Quality/example.fastq.bgz")
self.check_by_char("Quality/example.fastq", "Quality/example.fastq.bgz")
def test_iter_example_cor6(self):
"""Check iteration over GenBank/cor6_6.gb.bgz"""
self.check_by_line("GenBank/cor6_6.gb", "GenBank/cor6_6.gb.bgz")
self.check_by_char("GenBank/cor6_6.gb", "GenBank/cor6_6.gb.bgz")
def test_iter_example_gb(self):
"""Check iteration over GenBank/NC_000932.gb.bgz"""
self.check_by_line("GenBank/NC_000932.gb", "GenBank/NC_000932.gb.bgz")
self.check_by_char("GenBank/NC_000932.gb", "GenBank/NC_000932.gb.bgz")
def test_bam_ex1(self):
"""Reproduce BGZF compression for BAM file"""
temp_file = self.temp_file
# Note this example is from an old version of samtools
# and all the blocks are full (except the last one)
self.rewrite("SamBam/ex1.bam", temp_file)
# Now check the blocks agree (using the fact that
# this example BAM file has simple block usage)
self.check_blocks("SamBam/ex1.bam", temp_file)
def test_iter_bam_ex1(self):
"""Check iteration over SamBam/ex1.bam"""
self.check_by_char("SamBam/ex1.bam", "SamBam/ex1.bam", True)
def test_example_fastq(self):
"""Reproduce BGZF compression for a FASTQ file"""
temp_file = self.temp_file
self.rewrite("Quality/example.fastq.gz", temp_file)
self.check_blocks("Quality/example.fastq.bgz", temp_file)
def test_example_gb(self):
"""Reproduce BGZF compression for NC_000932 GenBank file"""
temp_file = self.temp_file
self.rewrite("GenBank/NC_000932.gb.bgz", temp_file)
self.check_blocks("GenBank/NC_000932.gb.bgz", temp_file)
def test_example_cor6(self):
"""Reproduce BGZF compression for cor6_6.gb GenBank file"""
temp_file = self.temp_file
self.rewrite("GenBank/cor6_6.gb.bgz", temp_file)
self.check_blocks("GenBank/cor6_6.gb.bgz", temp_file)
def test_example_wnts_xml(self):
"""Reproduce BGZF compression for wnts.xml BLAST file"""
temp_file = self.temp_file
self.rewrite("Blast/wnts.xml.bgz", temp_file)
self.check_blocks("Blast/wnts.xml.bgz", temp_file)
def test_write_tell(self):
"""Check offset works during BGZF writing"""
temp_file = self.temp_file
h = bgzf.open(temp_file, "w") # Text mode!
# When opening new file, offset should be 0
self.assertEqual(h.tell(), 0)
h.write("X" * 100000)
offset = h.tell()
self.assertNotEqual(offset, 100000) # Should be a virtual offset!
# After writing the same data two times, size of the first and the second
# write should be equal also in terms of offsets
# (This is because the flush ensures two identical blocks written)
h.flush()
offset1 = h.tell()
# Note 'offset' and 'offset1' effectively the same, but not equal
# due to the flush - 'offet' is at the end of the first BGZF block,
# while 'offset1' is at the start of the second BGZF block. In terms
# of the decompressed data, they point to the same location!
self.assertNotEqual(offset, offset1) # New block started
h.write("Magic" + "Y" * 100000)
h.flush()
offset2 = h.tell()
h.write("Magic" + "Y" * 100000)
h.flush()
offset3 = h.tell()
self.assertEqual(((offset3 << 16) - (offset2 << 16)),
((offset2 << 16) - (offset1 << 16)))
# Flushing should change the offset
h.flush()
self.assertNotEqual(offset3, h.tell())
h.close()
h = bgzf.open(temp_file, "r") # Text mode!
h.seek(offset) # i.e. End of first BGZF block
self.assertEqual(offset1, h.tell()) # Note *not* seek offset
# Now at start of second BGZF block
self.assertEqual(h.read(5), "Magic")
h.seek(offset2)
self.assertEqual(offset2, h.tell())
self.assertEqual(h.read(5), "Magic")
# Now go back in the file,
h.seek(offset1)
self.assertEqual(offset1, h.tell())
self.assertEqual(h.read(5), "Magic")
h.close()
if __name__ == "__main__":
runner = unittest.TextTestRunner(verbosity=2)
unittest.main(testRunner=runner)
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