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BLASTP 2.0.11 [Jan-20-2000]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
(197 letters)
Database: data/swissprot
82,258 sequences; 29,652,561 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN 402 e-112
gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN I... 30 3.3
gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE 29 7.4
gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 1... 29 7.4
>gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
Length = 197
Score = 402 bits (1021), Expect = e-112
Identities = 197/197 (100%), Positives = 197/197 (100%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
Query: 181 RRFADRLMVKLNHIRIK 197
RRFADRLMVKLNHIRIK
Sbjct: 181 RRFADRLMVKLNHIRIK 197
>gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN IN
NMD5-HOM6 INTERGENIC REGION
Length = 1442
Score = 30.1 bits (66), Expect = 3.3
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 10/70 (14%)
Query: 107 TLMASKETLLKR--------LRTRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQ 158
TL+ K+ L R TR + K S AA D+ +EGLS P + +D +
Sbjct: 637 TLLTRKDPSLSRNLKQSAGDALTRKQEKRSKAA--FDQLLEGLSGPPLHVYYASDGGNAA 694
Query: 159 DVAENIAARA 168
++A+ +AARA
Sbjct: 695 NLAKRLAARA 704
>gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE
Length = 376
Score = 29.0 bits (63), Expect = 7.4
Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 2/37 (5%)
Query: 3 IWINGAFGSGKTQTAFELHRRLNP--SYVYDPEKMGF 37
++I+G G GKT T+ +L L P + VY PE M +
Sbjct: 52 VYIDGPHGVGKTTTSAQLMEALGPRDNIVYVPEPMTY 88
>gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 190
KD POLYPEPTIDE
Length = 1689
Score = 29.0 bits (63), Expect = 7.4
Identities = 12/38 (31%), Positives = 20/38 (52%)
Query: 75 GILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASK 112
G +++P+ HP +F+++ LR HHF L K
Sbjct: 78 GHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVK 115
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN 406 e-113
Sequences not found previously or not previously below threshold:
gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN I... 30 3.3
gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 1... 30 3.3
gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE 29 7.4
CONVERGED!
>gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
Length = 197
Score = 406 bits (1031), Expect = e-113
Identities = 197/197 (100%), Positives = 197/197 (100%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
Query: 181 RRFADRLMVKLNHIRIK 197
RRFADRLMVKLNHIRIK
Sbjct: 181 RRFADRLMVKLNHIRIK 197
>gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN IN
NMD5-HOM6 INTERGENIC REGION
Length = 1442
Score = 30.1 bits (66), Expect = 3.3
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 10/70 (14%)
Query: 107 TLMASKETLLKR--------LRTRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQ 158
TL+ K+ L R TR + K S AA D+ +EGLS P + +D +
Sbjct: 637 TLLTRKDPSLSRNLKQSAGDALTRKQEKRSKAA--FDQLLEGLSGPPLHVYYASDGGNAA 694
Query: 159 DVAENIAARA 168
++A+ +AARA
Sbjct: 695 NLAKRLAARA 704
>gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 190
KD POLYPEPTIDE
Length = 1689
Score = 30.1 bits (66), Expect = 3.3
Identities = 12/38 (31%), Positives = 20/38 (52%)
Query: 75 GILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASK 112
G +++P+ HP +F+++ LR HHF L K
Sbjct: 78 GHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVK 115
>gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE
Length = 376
Score = 28.9 bits (63), Expect = 7.4
Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 2/37 (5%)
Query: 3 IWINGAFGSGKTQTAFELHRRLNP--SYVYDPEKMGF 37
++I+G G GKT T+ +L L P + VY PE M +
Sbjct: 52 VYIDGPHGVGKTTTSAQLMEALGPRDNIVYVPEPMTY 88
Database: data/swissprot
Posted date: Feb 2, 2000 9:39 AM
Number of letters in database: 29,652,561
Number of sequences in database: 82,258
Lambda K H
0.318 0.134 0.412
Lambda K H
0.270 0.0471 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23669955
Number of Sequences: 82258
Number of extensions: 918938
Number of successful extensions: 2130
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2126
Number of HSP's gapped (non-prelim): 8
length of query: 197
length of database: 29,652,561
effective HSP length: 48
effective length of query: 149
effective length of database: 25,704,177
effective search space: 3829922373
effective search space used: 3829922373
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 62 (28.6 bits)
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