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BLASTP 2.2.26+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: minirefseq_prot
20 sequences; 6,406 total letters
Query= random_s00
Length=32
***** No hits found *****
Lambda K H
0.399 0.202 0.884
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 156650
Query= gi|16080617|ref|NP_391444.1| membrane bound lipoprotein [Bacillus
subtilis subsp. subtilis str. 168]
Length=102
Score E
Sequences producing significant alignments: (Bits) Value
gi|308175296|ref|YP_003922001.1| membrane bound lipoprotein [Ba... 139 2e-46
gi|375363999|ref|YP_005132038.1| lytA gene product [Bacillus am... 89.0 7e-27
gi|154687679|ref|YP_001422840.1| LytA [Bacillus amyloliquefacie... 89.0 8e-27
gi|311070071|ref|YP_003974994.1| unnamed protein product [Bacil... 83.2 1e-24
gi|332258565|ref|XP_003278367.1| PREDICTED: UPF0764 protein C16... 15.8 7.1
> gi|308175296|ref|YP_003922001.1| membrane bound lipoprotein [Bacillus
amyloliquefaciens DSM 7]
Length=100
Score = 139 bits (350), Expect = 2e-46, Method: Compositional matrix adjust.
Identities = 69/102 (68%), Positives = 81/102 (79%), Gaps = 2/102 (2%)
Query 1 MKKFIALLFFILLLSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVDNEPV 60
MKK LFFILLL+GCGV ++KSQGED + TKEGTYVGLADTHTIEVTVD+EPV
Sbjct 1 MKKIFGCLFFILLLAGCGVTNEKSQGEDAG--EKLVTKEGTYVGLADTHTIEVTVDHEPV 58
Query 61 SLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERAN 102
S DITEES D+ N+G+KVT+ Y+KN +GQL+LKDIE AN
Sbjct 59 SFDITEESADDVKNLNNGEKVTVKYQKNSKGQLVLKDIEPAN 100
> gi|375363999|ref|YP_005132038.1| lytA gene product [Bacillus
amyloliquefaciens subsp. plantarum CAU B946]
Length=105
Score = 89.0 bits (219), Expect = 7e-27, Method: Compositional matrix adjust.
Identities = 48/105 (46%), Positives = 69/105 (66%), Gaps = 5/105 (5%)
Query 1 MKKFIALLFFILL----LSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVD 56
MKK IA F ILL L+ CG Q +G S ++ + + YVG+ADTHTIEV VD
Sbjct 1 MKKTIAASFLILLFSVVLAACGTAEQSKKGSG-SSENQAQKETAYYVGMADTHTIEVKVD 59
Query 57 NEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERA 101
++PVS + +++ + L+KF+ DKV+ITY ND+GQ +K+IE+A
Sbjct 60 DQPVSFEFSDDFSDVLNKFSENDKVSITYFTNDKGQKEIKEIEKA 104
> gi|154687679|ref|YP_001422840.1| LytA [Bacillus amyloliquefaciens
FZB42]
Length=105
Score = 89.0 bits (219), Expect = 8e-27, Method: Compositional matrix adjust.
Identities = 48/105 (46%), Positives = 69/105 (66%), Gaps = 5/105 (5%)
Query 1 MKKFIALLFFILL----LSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVD 56
MKK IA F ILL L+ CG Q +G S ++ + + YVG+ADTHTIEV VD
Sbjct 1 MKKTIAASFLILLFSVVLAACGTADQSKKGSG-SSENQAQKETAYYVGMADTHTIEVKVD 59
Query 57 NEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERA 101
++PVS + +++ + L+KF+ DKV+ITY ND+GQ +K+IE+A
Sbjct 60 DQPVSFEFSDDFSDVLNKFSENDKVSITYFTNDKGQKEIKEIEKA 104
> gi|311070071|ref|YP_003974994.1| unnamed protein product [Bacillus
atrophaeus 1942]
Length=105
Score = 83.2 bits (204), Expect = 1e-24, Method: Compositional matrix adjust.
Identities = 45/104 (43%), Positives = 66/104 (63%), Gaps = 5/104 (5%)
Query 1 MKKFIALLFFILL----LSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVD 56
MKK +A F ILL L+ CG Q +G + S S ++ + YVG+ADTHTIEV +D
Sbjct 1 MKKNVASSFLILLFSIILAACGTAEQSKEG-NGSSSSQVQNETAYYVGMADTHTIEVKID 59
Query 57 NEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIER 100
++PVS + T++ + L++F DKV I+Y ND+GQ L +IE+
Sbjct 60 DQPVSFEFTDDFSEILNEFEENDKVNISYLTNDKGQKELTEIEK 103
> gi|332258565|ref|XP_003278367.1| PREDICTED: UPF0764 protein C16orf89-like
[Nomascus leucogenys]
Length=132
Score = 15.8 bits (29), Expect = 7.1, Method: Compositional matrix adjust.
Identities = 7/25 (28%), Positives = 11/25 (44%), Gaps = 0/25 (0%)
Query 60 VSLDITEESTSDLDKFNSGDKVTIT 84
V + + L+ SGD T+T
Sbjct 80 VEMGFLHVGQAGLELVTSGDPPTLT 104
Lambda K H
0.310 0.131 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 361344
Query= gi|11464971:4-101 pleckstrin [Mus musculus]
Length=98
Score E
Sequences producing significant alignments: (Bits) Value
gi|11464971|ref|NP_062422.1| pleckstrin [Mus musculus] 205 2e-69
gi|354480464|ref|XP_003502426.1| PREDICTED: pleckstrin-like [Cr... 205 3e-69
gi|156616273|ref|NP_002655.2| pleckstrin [Homo sapiens] 204 1e-68
gi|297667453|ref|XP_002811995.1| PREDICTED: pleckstrin-like [Po... 204 1e-68
gi|350596020|ref|XP_003360649.2| PREDICTED: pleckstrin-like [Su... 199 2e-68
gi|33188429|ref|NP_872601.1| histone demethylase UTY isoform 1 ... 18.5 0.88
gi|57113895|ref|NP_001009002.1| histone demethylase UTY [Pan tr... 18.5 0.88
> gi|11464971|ref|NP_062422.1| pleckstrin [Mus musculus]
Length=350
Score = 205 bits (522), Expect = 2e-69, Method: Compositional matrix adjust.
Identities = 98/98 (100%), Positives = 98/98 (100%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 43.5 bits (101), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 29/100 (29%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS--PCQD 58
I++G L+K+G WK +L ED + +Y P G + L+G +TS D
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAVHLRGCVVTSVESSHD 305
Query 59 FGK--RMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
K + +I T + ++ QAA +ER W++ I+ A
Sbjct 306 VKKSDEENLFEIITADEVHYYLQAATSKERTEWIKAIQVA 345
> gi|354480464|ref|XP_003502426.1| PREDICTED: pleckstrin-like [Cricetulus
griseus]
Length=350
Score = 205 bits (521), Expect = 3e-69, Method: Compositional matrix adjust.
Identities = 98/98 (100%), Positives = 98/98 (100%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 43.9 bits (102), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 50/100 (50%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDF- 59
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNHD 305
Query 60 GKRM---FVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
GK+ + +I T + ++ QAA +ER W++ I+ A
Sbjct 306 GKKSDDENLFEIITADEVHYYLQAAAPKERTEWIKAIQVA 345
> gi|156616273|ref|NP_002655.2| pleckstrin [Homo sapiens]
Length=350
Score = 204 bits (518), Expect = 1e-68, Method: Compositional matrix adjust.
Identities = 97/98 (99%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 47.4 bits (111), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN 305
Query 61 KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
R + +I T + +F QAA +ER W+R I+ A
Sbjct 306 GRKSEEENLFEIITADEVHYFLQAATPKERTEWIRAIQMA 345
> gi|297667453|ref|XP_002811995.1| PREDICTED: pleckstrin-like [Pongo
abelii]
Length=350
Score = 204 bits (518), Expect = 1e-68, Method: Compositional matrix adjust.
Identities = 97/98 (99%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 45.4 bits (106), Expect = 6e-10, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN 305
Query 61 KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
R + +I T + +F QAA +ER W++ I+ A
Sbjct 306 GRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 345
> gi|350596020|ref|XP_003360649.2| PREDICTED: pleckstrin-like [Sus
scrofa]
Length=228
Score = 199 bits (506), Expect = 2e-68, Method: Compositional matrix adjust.
Identities = 94/98 (96%), Positives = 96/98 (98%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGS+FNTWKPMWV+LLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSMFNTWKPMWVILLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERD WVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDGWVRDIKKAIK 101
Lambda K H
0.321 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 345626
Database: minirefseq_prot
Posted date: Jun 9, 2012 2:05 PM
Number of letters in database: 6,406
Number of sequences in database: 20
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
|
