1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255
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TBLASTX 2.2.26+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: NCBI Transcript Reference Sequences
2,903,055 sequences; 4,626,651,242 total letters
Query= gi|356995852:1-490 Mus musculus POU domain, class 5, transcription
factor 1 (Pou5f1), transcript variant 1, mRNA
Length=490
Score E
Sequences producing significant alignments: (Bits) Value N
ref|NM_013633.3| Mus musculus POU domain, class 5, transcription... 418 2e-115 1
ref|XR_141831.1| PREDICTED: Mus musculus predicted gene, 19553 (... 415 3e-114 1
ref|XR_107064.1| PREDICTED: Mus musculus hypothetical LOC1005051... 403 8e-111 1
ref|XR_105908.1| PREDICTED: Mus musculus hypothetical LOC1005051... 403 8e-111 1
ref|NM_001009178.2| Rattus norvegicus POU class 5 homeobox 1 (Po... 349 2e-94 1
ref|XM_528230.3| PREDICTED: Pan troglodytes POU class 5 homeobox... 126 2e-27 1
ref|XR_021762.2| PREDICTED: Pan troglodytes POU domain, class 5,... 125 4e-27 1
ref|NR_034180.1| Homo sapiens POU class 5 homeobox 1 pseudogene ... 125 4e-27 1
ref|XM_002752481.1| PREDICTED: Callithrix jacchus POU domain, cl... 125 5e-27 1
ref|XM_002746317.1| PREDICTED: Callithrix jacchus POU domain, cl... 125 5e-27 1
ref|XM_001135162.2| PREDICTED: Pan troglodytes POU class 5 homeo... 124 1e-26 1
ref|NM_001114955.1| Macaca mulatta POU class 5 homeobox 1 (POU5F... 124 1e-26 1
ref|NR_033594.1| Mus musculus predicted gene 5712 (Gm5712), non-... 124 1e-26 1
ref|NM_001252041.1| Pan troglodytes POU class 5 homeobox 1 (POU5... 124 1e-26 1
ref|XR_024993.2| PREDICTED: Pan troglodytes POU domain, class 5,... 124 1e-26 1
ref|NM_002701.4| Homo sapiens POU class 5 homeobox 1 (POU5F1), t... 124 1e-26 1
ref|XM_002809098.1| PREDICTED: Pongo abelii POU domain, class 5,... 122 5e-26 1
ref|XM_003505863.1| PREDICTED: Cricetulus griseus POU domain, cl... 121 1e-25 1
ref|NM_001173441.1| Felis catus POU class 5 homeobox 1 (POU5F1),... 120 1e-25 1
ref|NR_036440.1| Homo sapiens POU class 5 homeobox 1 pseudogene ... 120 2e-25 1
>ref|NM_013633.3| Mus musculus POU domain, class 5, transcription factor 1 (Pou5f1),
transcript variant 1, mRNA
Length=1353
Score = 418 bits (908), Expect = 2e-115
Identities = 163/163 (100%), Positives = 163/163 (100%), Gaps = 0/163 (0%)
Frame = +1/+1
Query 1 EVKPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*AS 180
EVKPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*AS
Sbjct 1 EVKPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*AS 180
Query 181 KGLQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHE 360
KGLQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHE
Sbjct 181 KGLQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHE 360
Query 361 WKATQREPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N 489
WKATQREPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N
Sbjct 361 WKATQREPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N 489
>ref|XR_141831.1| PREDICTED: Mus musculus predicted gene, 19553 (Gm19553), miscRNA
ref|XR_105837.2| PREDICTED: Mus musculus predicted gene, 19553 (Gm19553), miscRNA
ref|XR_141464.1| PREDICTED: Mus musculus predicted gene, 19553 (Gm19553), miscRNA
ref|XR_141446.1| PREDICTED: Mus musculus predicted gene, 19553 (Gm19553), miscRNA
Length=570
Score = 415 bits (900), Expect = 3e-114
Identities = 162/163 (99%), Positives = 162/163 (99%), Gaps = 0/163 (0%)
Frame = +1/-1
Query 1 EVKPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*AS 180
EVKPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*AS
Sbjct 570 EVKPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*AS 391
Query 181 KGLQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHE 360
KGLQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHE
Sbjct 390 KGLQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHE 211
Query 361 WKATQREPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N 489
WKATQREPPLSPVPTAPMP*SWRRWNQL RSPRT*KPCRRS*N
Sbjct 210 WKATQREPPLSPVPTAPMP*SWRRWNQLQRSPRT*KPCRRS*N 82
>ref|XR_107064.1| PREDICTED: Mus musculus hypothetical LOC100505104 (LOC100505104),
partial miscRNA
Length=505
Score = 403 bits (875), Expect = 8e-111
Identities = 157/158 (99%), Positives = 157/158 (99%), Gaps = 0/158 (0%)
Frame = +1/-1
Query 16 LGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKGLQV 195
LGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKGLQV
Sbjct 505 LGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKGLQV 326
Query 196 GLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWKATQ 375
GLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWKATQ
Sbjct 325 GLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWKATQ 146
Query 376 REPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N 489
REPPLSPVPTAPMP*SWRRWNQL RSPRT*KPCRRS*N
Sbjct 145 REPPLSPVPTAPMP*SWRRWNQLQRSPRT*KPCRRS*N 32
>ref|XR_105908.1| PREDICTED: Mus musculus hypothetical LOC100505104 (LOC100505104),
partial miscRNA
Length=505
Score = 403 bits (875), Expect = 8e-111
Identities = 157/158 (99%), Positives = 157/158 (99%), Gaps = 0/158 (0%)
Frame = +1/-1
Query 16 LGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKGLQV 195
LGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKGLQV
Sbjct 505 LGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKGLQV 326
Query 196 GLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWKATQ 375
GLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWKATQ
Sbjct 325 GLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWKATQ 146
Query 376 REPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N 489
REPPLSPVPTAPMP*SWRRWNQL RSPRT*KPCRRS*N
Sbjct 145 REPPLSPVPTAPMP*SWRRWNQLQRSPRT*KPCRRS*N 32
>ref|NM_001009178.2| Rattus norvegicus POU class 5 homeobox 1 (Pou5f1), mRNA
gb|BC158566.1| Rattus norvegicus POU class 5 homeobox 1, mRNA (cDNA clone MGC:187285
IMAGE:9092936), complete cds
Length=1388
Score = 349 bits (757), Expect = 2e-94
Identities = 138/161 (86%), Positives = 143/161 (89%), Gaps = 0/161 (0%)
Frame = +1/+1
Query 7 KPSLGEPSFHQAPGSGCPPSPWLDTWLQTSPSHPHQVGVMGQQGWSRAGWILEPG*ASKG 186
K SLGE SFHQAPGSG PPSPWLDTWLQTSPSHPH VGVMGQQGWSRAGW LEPG*AS+G
Sbjct 19 KQSLGESSFHQAPGSGSPPSPWLDTWLQTSPSHPHLVGVMGQQGWSRAGWTLEPG*ASRG 198
Query 187 LQVGLESDQAQRYWGSPHVRPHTSSAEGWHTVDLRLDWA*SPKLAWRLCSLRARQEHEWK 366
LQVGLESDQ QR WGSP V HTSS EGWHTVDLRLDWA*SPKLAWRLCS RARQEHEW+
Sbjct 199 LQVGLESDQVQRCWGSPRVPQHTSSVEGWHTVDLRLDWA*SPKLAWRLCSPRARQEHEWR 378
Query 367 ATQREPPLSPVPTAPMP*SWRRWNQLPRSPRT*KPCRRS*N 489
AT+REPPL PV AP P*SWRRWN +PRSPR *KPCRRS*+
Sbjct 379 ATRREPPLGPVLPAPAP*SWRRWNLVPRSPRI*KPCRRS*S 501
>ref|XM_528230.3| PREDICTED: Pan troglodytes POU class 5 homeobox 1B (POU5F1B),
mRNA
Length=1601
Score = 126 bits (274), Expect = 2e-27
Identities = 53/73 (73%), Positives = 59/73 (81%), Gaps = 0/73 (0%)
Frame = -1/-2
Query 433 TFSNFTALGRSAQGSEEVPSELLSTRAPAWPSGCKVSTPTWGTRPSPT*GPQYAIPPQNS 254
+FS+FTA G + QGS E PSELLST PA PSG +VS P WGT P+PT*GPQYAIPPQNS
Sbjct 640 SFSSFTAPGGTVQGSGEAPSELLSTPTPASPSG*EVSKPPWGTSPAPT*GPQYAIPPQNS 461
Query 253 YAGGHGEIPNTSE 215
Y GGHG IP+TSE
Sbjct 460 YGGGHGGIPHTSE 422
>ref|XR_021762.2| PREDICTED: Pan troglodytes POU domain, class 5, transcription
factor 1-like (LOC741863), miscRNA
Length=1218
Score = 125 bits (272), Expect = 4e-27
Identities = 53/73 (73%), Positives = 60/73 (82%), Gaps = 0/73 (0%)
Frame = -1/-2
Query 433 TFSNFTALGRSAQGSEEVPSELLSTRAPAWPSGCKVSTPTWGTRPSPT*GPQYAIPPQNS 254
+FS+FTA G + QGS EVPSELLST PA PSG +VS P WGT +PT*GPQYAIPPQNS
Sbjct 380 SFSSFTAPGGTVQGSREVPSELLSTLTPASPSG*EVSKPPWGTSRTPT*GPQYAIPPQNS 201
Query 253 YAGGHGEIPNTSE 215
Y+GGHG IP+TSE
Sbjct 200 YSGGHGGIPHTSE 162
>ref|NR_034180.1| Homo sapiens POU class 5 homeobox 1 pseudogene 4 (POU5F1P4),
non-coding RNA
Length=1083
Score = 125 bits (272), Expect = 4e-27
Identities = 53/73 (73%), Positives = 60/73 (82%), Gaps = 0/73 (0%)
Frame = -1/-1
Query 433 TFSNFTALGRSAQGSEEVPSELLSTRAPAWPSGCKVSTPTWGTRPSPT*GPQYAIPPQNS 254
+FS+FTA G + QGS EVPSELLST PA PSG +VS P WGT +PT*GPQYAIPPQNS
Sbjct 381 SFSSFTAPGGTVQGSREVPSELLSTLTPASPSG*EVSKPPWGTSRTPT*GPQYAIPPQNS 202
Query 253 YAGGHGEIPNTSE 215
Y+GGHG IP+TSE
Sbjct 201 YSGGHGGIPHTSE 163
>ref|XM_002752481.1| PREDICTED: Callithrix jacchus POU domain, class 5, transcription
factor 1-like (LOC100389924), mRNA
Length=1368
Score = 125 bits (271), Expect = 5e-27
Identities = 53/72 (74%), Positives = 58/72 (81%), Gaps = 0/72 (0%)
Frame = -1/-2
Query 433 TFSNFTALGRSAQGSEEVPSELLSTRAPAWPSGCKVSTPTWGTRPSPT*GPQYAIPPQNS 254
+FS+FTA G QGS E PSELLST APA PSG +VS P WGT P+PT*GPQYAIPPQNS
Sbjct 416 SFSSFTAPGGMVQGSGEAPSELLSTPAPASPSG*EVSKPPWGTSPTPT*GPQYAIPPQNS 237
Query 253 YAGGHGEIPNTS 218
Y GGHG IP+TS
Sbjct 236 YGGGHGGIPHTS 201
>ref|XM_002746317.1| PREDICTED: Callithrix jacchus POU domain, class 5, transcription
factor 1-like (LOC100384946), mRNA
Length=1417
Score = 125 bits (271), Expect = 5e-27
Identities = 53/72 (74%), Positives = 58/72 (81%), Gaps = 0/72 (0%)
Frame = -1/-1
Query 433 TFSNFTALGRSAQGSEEVPSELLSTRAPAWPSGCKVSTPTWGTRPSPT*GPQYAIPPQNS 254
+FS+FTA G QGS E PSELLST APA PSG +VS P WGT P+PT*GPQYAIPPQNS
Sbjct 466 SFSSFTAPGGMVQGSGEAPSELLSTPAPASPSG*EVSKPPWGTSPTPT*GPQYAIPPQNS 287
Query 253 YAGGHGEIPNTS 218
Y GGHG IP+TS
Sbjct 286 YGGGHGGIPHTS 251
Lambda K H
0.318 0.134 0.401
Effective search space used: 149315297940
Database: NCBI Transcript Reference Sequences
Posted date: Mar 1, 2012 8:32 PM
Number of letters in database: 4,626,651,242
Number of sequences in database: 2,903,055
Matrix: BLOSUM62
Neighboring words threshold: 13
Window for multiple hits: 40
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