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TBLASTX 2.2.26+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: minirefseq_mrna
23 sequences; 67,750 total letters
Query= random_s00
Length=128
***** No hits found *****
Effective search space used: 0
Query= gi|296147483:1-350 Saccharomyces cerevisiae S288c Mon2p (MON2) mRNA,
complete cds
Length=350
Score E
Sequences producing significant alignments: (Bits) Value N
gi|296147483|ref|NM_001183135.1| Saccharomyces cerevisiae S288c... 289 2e-81 1
gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS 42... 152 5e-42 2
gi|366988334|ref|XM_003673886.1| Naumovozyma castellii CBS 4309... 143 3e-37 1
gi|255710474|ref|XM_002551475.1| Lachancea thermotolerans CBS 6... 141 8e-37 1
gi|254579534|ref|XM_002495708.1| Zygosaccharomyces rouxii hypot... 141 1e-36 1
gi|350596019|ref|XM_003360601.2| PREDICTED: Sus scrofa pleckstr... 25.4 0.091 1
gi|332865372|ref|XM_003318468.1| PREDICTED: Pan troglodytes zin... 23.5 0.33 1
gi|145479850|ref|XM_001425911.1| Paramecium tetraurelia hypothe... 21.7 1.2 1
gi|94721341|ref|NM_001040441.1| Homo sapiens zinc finger and BT... 21.2 1.3 2
gi|332237160|ref|XM_003267724.1| PREDICTED: Nomascus leucogenys... 19.9 4.1 1
> gi|296147483|ref|NM_001183135.1| Saccharomyces cerevisiae S288c
Mon2p (MON2) mRNA, complete cds
Length=4911
Score = 289 bits (626), Expect = 2e-81
Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
Frame = +2/+2
Query 2 WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISH 181
WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISH
Sbjct 2 WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISH 181
Query 182 SLSCWLANQGMLK*RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K 349
SLSCWLANQGMLK*RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K
Sbjct 182 SLSCWLANQGMLK*RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K 349
Score = 278 bits (602), Expect = 5e-78
Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
Frame = -2/-3
Query 349 LLIESPSRDE*PQ*RHPKFQTAGFEE*MERLTVPVGIALPGSSF*HSLIGKPTRKGVRNP 170
LLIESPSRDE*PQ*RHPKFQTAGFEE*MERLTVPVGIALPGSSF*HSLIGKPTRKGVRNP
Sbjct 349 LLIESPSRDE*PQ*RHPKFQTAGFEE*MERLTVPVGIALPGSSF*HSLIGKPTRKGVRNP 170
Query 169 DVFLAPQNYVLFSISQWIYH*HGEWLNFFFSIRKIKNAILLQVAFAWSQTLQCSWP 2
DVFLAPQNYVLFSISQWIYH*HGEWLNFFFSIRKIKNAILLQVAFAWSQTLQCSWP
Sbjct 169 DVFLAPQNYVLFSISQWIYH*HGEWLNFFFSIRKIKNAILLQVAFAWSQTLQCSWP 2
Score = 274 bits (593), Expect = 8e-77
Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
Frame = -3/-1
Query 348 F*LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIR 169
F*LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIR
Sbjct 348 F*LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIR 169
Query 168 MSF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH 1
MSF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH
Sbjct 168 MSF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH 1
Score = 270 bits (583), Expect = 2e-75
Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
Frame = +3/+3
Query 3 GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIEKST*F*GARKTSGFRT 182
GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIEKST*F*GARKTSGFRT
Sbjct 3 GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIEKST*F*GARKTSGFRT 182
Query 183 PFRVGLPIKEC*NDDPGNAMPTGTVNRSIYSSKPAV*NFGCLH*GYSSRDGDSIKS 350
PFRVGLPIKEC*NDDPGNAMPTGTVNRSIYSSKPAV*NFGCLH*GYSSRDGDSIKS
Sbjct 183 PFRVGLPIKEC*NDDPGNAMPTGTVNRSIYSSKPAV*NFGCLH*GYSSRDGDSIKS 350
Score = 229 bits (495), Expect = 3e-63
Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
Frame = -1/-2
Query 350 TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTKgsaks 171
TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTKGSAKS
Sbjct 350 TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTKGSAKS 171
Query 170 gclssssklCTLFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESNPPVFMA 3
GCLSSSSKLCTLFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESNPPVFMA
Sbjct 170 GCLSSSSKLCTLFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESNPPVFMA 3
Score = 197 bits (425), Expect(2) = 2e-58
Identities = 85/85 (100%), Positives = 85/85 (100%), Gaps = 0/85 (0%)
Frame = +1/+1
Query 94 TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI 273
TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI
Sbjct 94 TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI 273
Query 274 PRSRLSEILDAFIEATHLAMEIQLK 348
PRSRLSEILDAFIEATHLAMEIQLK
Sbjct 274 PRSRLSEILDAFIEATHLAMEIQLK 348
Score = 36.3 bits (73), Expect(2) = 2e-58
Identities = 14/14 (100%), Positives = 14/14 (100%), Gaps = 0/14 (0%)
Frame = +1/+1
Query 1 MAMNTGGFDSMQRQ 42
MAMNTGGFDSMQRQ
Sbjct 1 MAMNTGGFDSMQRQ 42
Score = 18.9 bits (35), Expect = 7.8
Identities = 6/11 (55%), Positives = 9/11 (82%), Gaps = 0/11 (0%)
Frame = +2/-3
Query 293 KFWMPSLRLLI 325
K W+P ++LLI
Sbjct 373 KKWVPPVKLLI 341
> gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS
421 hypothetical protein (NDAI0A06120), mRNA
Length=4932
Score = 152 bits (327), Expect(2) = 5e-42
Identities = 62/85 (73%), Positives = 73/85 (86%), Gaps = 0/85 (0%)
Frame = +1/+1
Query 94 TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI 273
TI+HASDKSI+ILK + + EEL RHPDF P VLAC SRNAK+T++AMQCLQGL++VPSI
Sbjct 88 TIKHASDKSIDILKTIQNIEELVRHPDFVTPLVLACSSRNAKLTSIAMQCLQGLASVPSI 267
Query 274 PRSRLSEILDAFIEATHLAMEIQLK 348
P SR+ E+LD FIEAT LAMEIQLK
Sbjct 268 PESRIPEVLDGFIEATQLAMEIQLK 342
Score = 26.3 bits (51), Expect(2) = 5e-42
Identities = 11/14 (79%), Positives = 11/14 (79%), Gaps = 0/14 (0%)
Frame = +3/+3
Query 69 FRIEKKKFNHSPC* 110
FRI KKKFNH C*
Sbjct 63 FRI*KKKFNH*TC* 104
Score = 68.0 bits (142), Expect(2) = 1e-24
Identities = 34/55 (62%), Positives = 38/55 (69%), Gaps = 0/55 (0%)
Frame = -1/-2
Query 350 TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTK 186
TFN ISIA *VASM S+ S GI+GT +PCRHC+A V LAFLD QA TK
Sbjct 344 TFNCISIAS*VASMNPSRTSGILDSGIEGTDANPCRHCMAIEVNLAFLDEQARTK 180
Score = 52.8 bits (109), Expect(2) = 1e-24
Identities = 24/39 (62%), Positives = 29/39 (74%), Gaps = 0/39 (0%)
Frame = -1/-2
Query 137 LFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESN 21
+F IS+DLSLA MVEFLL DSE++E NS S+C E N
Sbjct 131 VFRISIDLSLACLMVEFLLLDSEERECNSWSNCFRKEVN 15
Score = 61.1 bits (127), Expect = 2e-12
Identities = 36/103 (35%), Positives = 52/103 (50%), Gaps = 0/103 (0%)
Frame = +2/+2
Query 41 NLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISHSLSCWLANQGMLK 220
N +N +L N KEEIQP ML N +*+ + + ++ I L W ++ M
Sbjct 35 NWTRNYTPFLQNLKEEIQPLNMLATNQSIF*RLFKI*KNL*GTLILLHLWSWPVHREMPN 214
Query 221 *RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K 349
* CNAY+D +P+ + KF SL+ L RWR+N*K
Sbjct 215 *LQLPCNAYKDWRPYLQYQSQESQKF*TDSLKPLS*RWRYN*K 343
Score = 42.8 bits (87), Expect = 5e-07
Identities = 28/65 (43%), Positives = 36/65 (55%), Gaps = 0/65 (0%)
Frame = -3/-1
Query 261 G*QSL*ALHCQGRHFSIP*LASQHERECEIRMSF*LLKTMYSFQYLNGFITSMANG*ISS 82
G QSL*ALH F I Q +R +IR+ + + S ++ F+ SM NG*ISS
Sbjct 255 GRQSL*ALHGN*S*FGISR*TGQDQRCNKIRVPYKFFYILNSL*NID*FVASMFNG*ISS 76
Query 81 FRFGR 67
FRF R
Sbjct 75 FRF*R 61
Score = 24.0 bits (46), Expect(2) = 0.028
Identities = 11/17 (65%), Positives = 11/17 (65%), Gaps = 0/17 (0%)
Frame = -2/-3
Query 118 IYH*HGEWLNFFFSIRK 68
I *H WLNFFF I K
Sbjct 112 ICR*HV*WLNFFF*ILK 62
Score = 23.5 bits (45), Expect(2) = 0.028
Identities = 9/17 (53%), Positives = 11/17 (65%), Gaps = 0/17 (0%)
Frame = -3/-2
Query 153 LKTMYSFQYLNGFITSM 103
L +SF + NGF TSM
Sbjct 3263 LSASHSFSFKNGFNTSM 3213
Score = 21.7 bits (41), Expect = 1.2
Identities = 9/30 (30%), Positives = 16/30 (53%), Gaps = 0/30 (0%)
Frame = +2/-2
Query 167 IRISHSLSCWLANQGMLK*RPWQCNAYRDC 256
I I SL+C + +L +CN++ +C
Sbjct 122 ISIDLSLACLMVEFLLLDSEERECNSWSNC 33
Score = 18.9 bits (35), Expect = 7.8
Identities = 6/11 (55%), Positives = 9/11 (82%), Gaps = 0/11 (0%)
Frame = +3/+3
Query 306 LH*GYSSRDGD 338
+H* +S+ DGD
Sbjct 300 IH*SHSASDGD 332
> gi|366988334|ref|XM_003673886.1| Naumovozyma castellii CBS 4309
hypothetical protein (NCAS0A09950) mRNA, complete cds
Length=4938
Score = 143 bits (306), Expect = 3e-37
Identities = 58/85 (68%), Positives = 71/85 (84%), Gaps = 0/85 (0%)
Frame = +1/+1
Query 94 TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI 273
+I+HASDKSIEILK + + E+L HPDF P V +C SRNAK+T++AMQCLQGL++ PSI
Sbjct 94 SIKHASDKSIEILKTIQNIEDLASHPDFVTPLVESCLSRNAKLTSIAMQCLQGLASAPSI 273
Query 274 PRSRLSEILDAFIEATHLAMEIQLK 348
P SRLS +LD FIEATHLA+EIQLK
Sbjct 274 PESRLSGVLDGFIEATHLAIEIQLK 348
Score = 62.5 bits (130), Expect(2) = 1e-20
Identities = 30/54 (56%), Positives = 36/54 (67%), Gaps = 0/54 (0%)
Frame = -1/-2
Query 350 TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANT 189
TF+ ISIAR VAS+ S+ DS L G+DG PC+HCIA V LAFLD +T
Sbjct 350 TFSCISIARCVASINPSRTPDSLLSGMDGADAKPCKHCIAIEVNLAFLDRHDST 189
Score = 44.6 bits (91), Expect(2) = 1e-20
Identities = 20/33 (61%), Positives = 24/33 (73%), Gaps = 0/33 (0%)
Frame = -1/-2
Query 137 LFNISMDLSLAWRMVEFLLFDSEDKERNSASSC 39
+F IS+DLSLA + FLL DSED E NS S+C
Sbjct 137 VFKISIDLSLACLIEVFLLLDSEDNECNSRSNC 39
Score = 54.2 bits (112), Expect = 2e-10
Identities = 38/115 (33%), Positives = 58/115 (50%), Gaps = 0/115 (0%)
Frame = -3/-1
Query 345 *LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIRM 166
*L + +M + I+N R A R W +L* LH S+P* ++ +IRM
Sbjct 345 *LYFNCKMCRFYKSIENSR*SAFRYGWCRC*AL*TLHSNRGQLSVP**T*FYQWSDKIRM 166
Query 165 SF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH 1
+ + + SF L+ +T M + + SFRFGR QF +L L G+K S++ H
Sbjct 165 ACQIFNVLDSF*DLDRLVTCMLDRSVPSFRFGRQ*MQF*VQLFLKGLKTCSLNSH 1
Score = 29.5 bits (58), Expect = 0.005
Identities = 18/45 (40%), Positives = 22/45 (49%), Gaps = 0/45 (0%)
Frame = +2/+2
Query 2 WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*K 136
W L+ L P K N NC+ LPN KE +M V + R *K
Sbjct 2 WLFKLQVLRPFKNNWT*NCIHCLPNLKEGTLLSSMQVTSLSRS*K 136
Score = 29.0 bits (57), Expect = 0.007
Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 0/44 (0%)
Frame = +3/+3
Query 3 GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIE 134
G ++R * +K RIAF +FRI KK+ + C * ++ D++
Sbjct 3 GCSNYRF*DLSKTIGPRIAFIVFRI*KKEHFYQACK*QVYRDLK 134
Score = 22.1 bits (42), Expect = 0.84
Identities = 10/17 (59%), Positives = 11/17 (65%), Gaps = 0/17 (0%)
Frame = -2/-3
Query 85 FFSIRKIKNAILLQVAF 35
FF IRK NAIL + F
Sbjct 85 FF*IRKTMNAILGPIVF 35
Score = 20.3 bits (38), Expect = 3.0
Identities = 6/13 (46%), Positives = 8/13 (62%), Gaps = 0/13 (0%)
Frame = +2/-2
Query 278 EAGCLKFWMPSLR 316
+ CL FW+ S R
Sbjct 4217 QTSCLNFWVMSSR 4179
> gi|255710474|ref|XM_002551475.1| Lachancea thermotolerans CBS
6340 KLTH0A01342p (KLTH0A01342g) mRNA, complete cds
Length=4845
Score = 141 bits (303), Expect = 8e-37
Identities = 55/84 (65%), Positives = 71/84 (85%), Gaps = 0/84 (0%)
Frame = +1/+1
Query 97 IRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIP 276
+RHASDKSIEILK VH FE+L RHPDF PFVL+C S+NAK+T++++QCLQ +S+V IP
Sbjct 94 VRHASDKSIEILKTVHEFEDLPRHPDFVTPFVLSCASKNAKLTSVSVQCLQKMSSVNCIP 273
Query 277 RSRLSEILDAFIEATHLAMEIQLK 348
R+ ++LDAFI++THLA EIQLK
Sbjct 274 EDRIEDVLDAFIDSTHLAAEIQLK 345
Score = 45.1 bits (92), Expect = 1e-07
Identities = 25/55 (45%), Positives = 29/55 (53%), Gaps = 0/55 (0%)
Frame = -1/-2
Query 350 TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTK 186
T + IS AR V SM AS S G+ T D CRHC +V LAFL+ TK
Sbjct 347 TLSCISAARWVESMNASSTSSILSSGMQLTDDIFCRHCTETLVSLAFLEAHERTK 183
Score = 29.0 bits (57), Expect = 0.007
Identities = 22/70 (31%), Positives = 33/70 (47%), Gaps = 0/70 (0%)
Frame = -3/-1
Query 249 L*ALHCQGRHFSIP*LASQHERECEIRMSF*LLKTMYSFQYLNGFITSMANG*ISSFRFG 70
L*ALH S+ + ER ++ M + + + M SFQ L +T M + + R
Sbjct 246 L*ALH*DACELSVFGGTRKDERRYKVWMPWKVFEFMNSFQDLY*LVTCMPHLRAAPLRLR 67
Query 69 R*RTQFCFKL 40
R QFC +L
Sbjct 66 RKCVQFCEEL 37
Score = 21.2 bits (40), Expect = 1.6
Identities = 6/8 (75%), Positives = 7/8 (88%), Gaps = 0/8 (0%)
Frame = +2/+2
Query 233 QCNAYRDC 256
QCNAY+ C
Sbjct 230 QCNAYKRC 253
Score = 18.9 bits (35), Expect = 7.8
Identities = 5/10 (50%), Positives = 6/10 (60%), Gaps = 0/10 (0%)
Frame = -2/-2
Query 31 WSQTLQCSWP 2
WS + SWP
Sbjct 2951 WSDIMLISWP 2922
> gi|254579534|ref|XM_002495708.1| Zygosaccharomyces rouxii hypothetical
protein (ZYRO0C02266g) mRNA, complete cds
Length=4866
Score = 141 bits (302), Expect = 1e-36
Identities = 57/84 (68%), Positives = 72/84 (86%), Gaps = 0/84 (0%)
Frame = +1/+1
Query 97 IRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIP 276
IR+ASDKSIEILK VHS+EEL RHPDF +P V++C S+NAK+TT++MQC Q L+TVP IP
Sbjct 97 IRNASDKSIEILKVVHSYEELSRHPDFIVPLVMSCASKNAKLTTISMQCFQKLATVPCIP 276
Query 277 RSRLSEILDAFIEATHLAMEIQLK 348
+LS++LDAFIEA LAM+I+LK
Sbjct 277 VDKLSDVLDAFIEANQLAMDIKLK 348
Lambda K H
0.318 0.134 0.401
Effective search space used: 1954618
Database: minirefseq_mrna
Posted date: Jun 9, 2012 2:05 PM
Number of letters in database: 67,750
Number of sequences in database: 23
Matrix: BLOSUM62
Neighboring words threshold: 13
Window for multiple hits: 40
|
