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LOCUS SYNPUC19CV 2686 bp DNA circular SYN 17-MAY-2019
DEFINITION Cloning vector pUC19c, complete sequence.
ACCESSION L09137
VERSION L09137.2
KEYWORDS .
SOURCE Cloning vector pUC19c
ORGANISM Cloning vector pUC19c
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2686)
AUTHORS Yanisch-Perron,C., Vieira,J. and Messing,J.
TITLE Improved M13 phage cloning vectors and host strains: nucleotide
sequences of the M13mp18 and pUC19 vectors
JOURNAL Gene 33 (1), 103-119 (1985)
PUBMED 2985470
REFERENCE 2 (bases 1 to 2686)
AUTHORS Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
TITLE The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
to facilitate the use of sonicated DNA for nucleotide sequencing
JOURNAL Gene 68 (1), 139-149 (1988)
PUBMED 2851488
REFERENCE 3 (bases 1 to 2686)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
REFERENCE 4 (bases 1 to 2686)
AUTHORS Messing,J.
TITLE Direct Submission
JOURNAL Submitted (27-APR-1993) Department of Biochemistry, University of
Minnesota, St. Paul, MN 55108, USA
REFERENCE 5 (bases 1 to 2686)
AUTHORS Messing,J.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2002) Rutgers, The State University of New
Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
Piscataway, NJ 08854-8020, USA
REMARK Sequence update by submitter
COMMENT On Apr 11, 2002 this sequence version replaced L09137.1. These data
and their annotation were supplied to GenBank by Will Gilbert
under the auspices of the GenBank Currator Program. pUC19c -
Cloning vector (beta-galactosidase mRNA on complementary strand)
ENTRY PUC19C #TYPE DNA CIRCULAR TITLE pUC19c -
Cloning vector
(beta-galactosidase mRNA on complementary strand)
DATE 03-FEB-1986
#sequence 16-DEC-1986
ACCESSION VB0033
SOURCE artificial
COLLECTION ATCC 37254
REFERENCE
#number
#authors Norrander J., Kempe T., Messing J.
#journal Gene (1983) 26: 101-106
REFERENCE
#number 1
#authors Yanisch-Perron C., Vieira J., Messing J.
#journal Gene (1985) 33: 103-119
#comment shows the complete compiled sequence
REFERENCE
#number 2
#authors Chambers,S.P., et al.
#journal Gene (1988) 68: 139-149
#describes mutation at nt1308 and its effect on copy number
REFERENCE
#number
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector I-A-iv-20
COMMENT
This Sequence was obtained 3-MAR-1986 from J. Messing, Waksman
Institute, NJ on floppy disk.
Revised 16-DEC-1986 by F. Pfeiffer:
1062/3 'AT' to 'TA' to match revised sequence of PBR322
The beta-galactosidase mRNA sequence including the multiple
cloning site of M13mp19 is on the strand complementary to that
shown.
KEYWORDS
CROSSREFERENCE
#complement
VecBase(3):pUC19
#prerevised
GenBank(50):M11662, EMBL(11):ARPuc19
#parent
VecBase(3):pUC13, VecBase(3):M13mp19, VecSource(3):bGal19
PARENT
Features of pUC19c (2686 bp)
residue source
1- 137 2074-2210 pBR322
138- 237 2252-2351 pBR322
238- 395 1461-1304 (c) Lac-Operon
396- 452 57- 1 (c) polylinker of M13mp19
455- 682 1298-1071 (c) Lac-Operon
683-2686 2352-4355 pBR322
Conflict (cfl) and Mutations (mut):
pUC19c source
mut 1308 A G 2977 pBR322 linked to increased copy number
mut 1942 A G 3611 pBR322
mut 2243 T C 3912 pBR322
FEATURE
1629-2417 789-1 (c) Ap-R
b-lactamase POLYLINKER
HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
#resistance Ap
#indicator beta-galactosidase
SUMMARY pUC19c #length 2686 #checksum 4465.
FEATURES Location/Qualifiers
source 1..2686
/organism="Cloning vector pUC19c"
/mol_type="genomic DNA"
/db_xref="taxon:174689"
promoter complement(514..543)
/standard_name="lac prom"
promoter complement(2528..2556)
/standard_name="amp prom"
misc_feature <2644..159
/standard_name="cross origin trunc"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
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