1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
|
^SAMPLE=GSM700
!Sample_status = Public on Nov 28 2001
!Sample_title = SAGE_Duke_H247_Hypoxia
!Sample_type = sage
!Sample_anchor = NlaIII
!Sample_organism = Homo sapiens
!Sample_target_source = Brain, glioblastoma multiforme, cell-line H247
!Sample_tag_count = 72031
!Sample_description = This library represents a Cancer Genome Anatomy Project library, which was either produced through CGAP funding, or donated to CGAP.
!Sample_description = The Cancer Genome Anatomy Project (CGAP: http://cgap.nci.nih.gov) is an interdisciplinary program established and administered by the National Cancer Institute (NCI: http://www.nci.nih.gov) to generate the information and technological tools needed to decipher the molecular anatomy of the cancer cell.
!Sample_description = Cell line grown under 1.5% oxygen conditions for 24 hours prior to harvesting in zinc option media with 10% RBS and harvested at passage 102. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).
!Sample_description = Organ: brain
!Sample_description = Tissue_type: glioblastoma multiforme
!Sample_description = Cell_line: H247
!Sample_description = Lab host: DH10B
!Sample_description = Vector: pZErO-1
!Sample_description = Vector type: plasmid
!Sample_description = R. Site 1: Sph1
!Sample_description = R. Site 2: Sph1
!Sample_description = Library treatment: non-normalized
!Sample_description = Tissue description: Brain, Duke glioblastoma multiforme cell line, H247, grown under 1.5% oxygen conditions for 24 hours prior to harvesting.
!Sample_description = Tissue
!Sample_author = Gregory,J,Riggins
!Sample_author = Robert,L,Strausberg
!Sample_web_link = http://cgap.nci.nih.gov
!Sample_submission_date = Nov 28 2001
!Sample_submitter_name = Robert,L,Strausberg
!Sample_submitter_email = cgapbs-r@mail.nih.gov
!Sample_submitter_institute = National Cancer Institute
!Sample_submitter_department = Cancer Genome Anatomy Project
!Sample_submitter_city = Bethesda,MD,20892,USA
!Sample_submitter_phone = 301-496-1550
!Sample_submitter_web_link = http://cgap.nci.nih.gov/
!Sample_platform_id = GPL4
!Sample_series_id = GSE14
#TAG = Ten base SAGE tag, LINK_PRE:"http://www.ncbi.nlm.nih.gov/SAGE/SAGEtag.cgi?tag="
#COUNT = Absolute tag count
#TPM = Tags per million, or (1000000*COUNT)/(Total tags)
TAG COUNT TPM
TCCAAATCGA 520 7219.11
TACCATCAAT 434 6025.18
TTGGGGTTTC 389 5400.45
CCCATCGTCC 367 5095.03
GTGAAACCCC 365 5067.26
GGGGAAATCG 357 4956.2
CCTGTAATCC 346 4803.49
TGATTTCACT 334 4636.89
TGTGTTGAGA 315 4373.12
GCCCCCAATA 303 4206.52
CTAAGACTTC 279 3873.33
GCGACCGTCA 276 3831.68
TTGGTCCTCT 276 3831.68
CCTAGCTGGA 268 3720.62
GATGAGGAGA 251 3484.61
ACTTTTTCAA 244 3387.43
CCACTGCACT 223 3095.89
GTGTGTTTGT 223 3095.89
GAAATACAGT 218 3026.47
GCTTTATTTG 218 3026.47
|
