1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119
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########################################
# Program: needle
# Rundate: Fri 23 Jul 2021 22:45:41
# Commandline: needle
# -asequence seqa.fa
# -bsequence seqb.fa
# -datafile EBLOSUM62
# -gapopen 10
# -gapextend 0.5
# -nobrief
# -outfile stdout
# Align_format: srspair
# Report_file: stdout
########################################
#=======================================
#
# Aligned_sequences: 2
# 1: IXI_234
# 2: IXI_235
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 131
# Identity: 112/131 (85.5%)
# Similarity: 112/131 (85.5%)
# Gaps: 19/131 (14.5%)
# Score: 591.5
# Longest_Identity = 100.00%
# Longest_Similarity = 100.00%
# Shortest_Identity = 85.50%
# Shortest_Similarity = 85.50%
#
#=======================================
IXI_234 1 TSPASIRPPAGPSSRPAMVSSRRTRPSPPGPRRPTGRPCCSAAPRRPQAT 50
||||||||||||||| ||||||||||||||||||||||||||
IXI_235 1 TSPASIRPPAGPSSR---------RPSPPGPRRPTGRPCCSAAPRRPQAT 41
IXI_234 51 GGWKTCSGTCTTSTSTRHRGRSGWSARTTTAACLRASRKSMRAACSRSAG 100
|||||||||||||||||||||||| ||||||||||||||||
IXI_235 42 GGWKTCSGTCTTSTSTRHRGRSGW----------RASRKSMRAACSRSAG 81
IXI_234 101 SRPNRFAPTLMSSCITSTTGPPAWAGDRSHE 131
|||||||||||||||||||||||||||||||
IXI_235 82 SRPNRFAPTLMSSCITSTTGPPAWAGDRSHE 112
#=======================================
#
# Aligned_sequences: 2
# 1: IXI_234
# 2: IXI_236
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 131
# Identity: 120/131 (91.6%)
# Similarity: 120/131 (91.6%)
# Gaps: 4/131 ( 3.1%)
# Score: 618.0
# Longest_Identity = 94.49%
# Longest_Similarity = 94.49%
# Shortest_Identity = 91.60%
# Shortest_Similarity = 91.60%
#
#=======================================
IXI_234 1 TSPASIRPPAGPSSRPAMVSSRRTRPSPPGPRRPTGRPCCSAAPRRPQAT 50
|||||||||||||||||||||| |||||.||||.|||||||||.|||||
IXI_236 1 TSPASIRPPAGPSSRPAMVSSR--RPSPPPPRRPPGRPCCSAAPPRPQAT 48
IXI_234 51 GGWKTCSGTCTTSTSTRHRGRSGWSARTTTAACLRASRKSMRAACSRSAG 100
||||||||||||||||||||||||||||||||||||||||||||||| |
IXI_236 49 GGWKTCSGTCTTSTSTRHRGRSGWSARTTTAACLRASRKSMRAACSR--G 96
IXI_234 101 SRPNRFAPTLMSSCITSTTGPPAWAGDRSHE 131
|||.||||.|||||||||||||..|||||||
IXI_236 97 SRPPRFAPPLMSSCITSTTGPPPPAGDRSHE 127
#=======================================
#
# Aligned_sequences: 2
# 1: IXI_234
# 2: IXI_237
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 131
# Identity: 119/131 (90.8%)
# Similarity: 124/131 (94.7%)
# Gaps: 7/131 ( 5.3%)
# Score: 609.0
# Longest_Identity = 95.97%
# Longest_Similarity = 100.00%
# Shortest_Identity = 90.84%
# Shortest_Similarity = 94.66%
#
#=======================================
IXI_234 1 TSPASIRPPAGPSSRPAMVSSRRTRPSPPGPRRPTGRPCCSAAPRRPQAT 50
|||||:||||||||||||||||| ||||||||||| |||||||||||
IXI_237 1 TSPASLRPPAGPSSRPAMVSSRR-RPSPPGPRRPT----CSAAPRRPQAT 45
IXI_234 51 GGWKTCSGTCTTSTSTRHRGRSGWSARTTTAACLRASRKSMRAACSRSAG 100
||:||||||||||||||||||||:||||||||||||||||||||||| |
IXI_237 46 GGYKTCSGTCTTSTSTRHRGRSGYSARTTTAACLRASRKSMRAACSR--G 93
IXI_234 101 SRPNRFAPTLMSSCITSTTGPPAWAGDRSHE 131
||||||||||||||:||||||||:|||||||
IXI_237 94 SRPNRFAPTLMSSCLTSTTGPPAYAGDRSHE 124
#---------------------------------------
#---------------------------------------
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