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#!/usr/bin/env python
#file test_parse.py
__author__ = "Rob Knight"
__copyright__ = "Copyright 2011, The QIIME Project"
__credits__ = ["Rob Knight", "Justin Kuczynski", "Greg Caporaso",\
"Cathy Lozupone", "Jens Reeder", "Daniel McDonald"] #remember to add yourself
__license__ = "GPL"
__version__ = "1.4.0"
__maintainer__ = "Greg Caporaso"
__email__ = "gregcaporaso@gmail.com"
__status__ = "Release"
from numpy import array, nan
from StringIO import StringIO
from cogent.util.unit_test import TestCase,main
from cogent.util.misc import remove_files
from qiime.util import get_tmp_filename
from qiime.parse import (group_by_field, group_by_fields,
parse_distmat, parse_rarefaction_record, parse_rarefaction, parse_coords,
parse_otu_table, make_envs_dict, fields_to_dict, parse_rarefaction_fname,
parse_qiime_parameters, parse_qiime_config_files,
parse_bootstrap_support, parse_sample_mapping, parse_distmat_to_dict,
sample_mapping_to_otu_table, parse_taxonomy, parse_mapping_file,
parse_metadata_state_descriptions, parse_rarefaction_data,
parse_illumina_line, parse_qual_score, parse_qual_scores, QiimeParseError,
parse_newick,parse_trflp,parse_taxa_summary_table, parse_prefs_file,
parse_mapping_file_to_dict, mapping_file_to_dict, MinimalQualParser,
parse_denoiser_mapping)
class TopLevelTests(TestCase):
"""Tests of top-level functions"""
def setUp(self):
"""define some top-level data"""
self.l19_data = array([
[7,1,0,0,0,0,0,0,0],
[4,2,0,0,0,1,0,0,0],
[2,4,0,0,0,1,0,0,0],
[1,7,0,0,0,0,0,0,0],
[0,8,0,0,0,0,0,0,0],
[0,7,1,0,0,0,0,0,0],
[0,4,2,0,0,0,2,0,0],
[0,2,4,0,0,0,1,0,0],
[0,1,7,0,0,0,0,0,0],
[0,0,8,0,0,0,0,0,0],
[0,0,7,1,0,0,0,0,0],
[0,0,4,2,0,0,0,3,0],
[0,0,2,4,0,0,0,1,0],
[0,0,1,7,0,0,0,0,0],
[0,0,0,8,0,0,0,0,0],
[0,0,0,7,1,0,0,0,0],
[0,0,0,4,2,0,0,0,4],
[0,0,0,2,4,0,0,0,1],
[0,0,0,1,7,0,0,0,0]
])
self.l19_sample_names = ['sam1', 'sam2', 'sam3', 'sam4', 'sam5','sam6',\
'sam7', 'sam8', 'sam9', 'sam_middle', 'sam11', 'sam12', 'sam13', \
'sam14', 'sam15', 'sam16', 'sam17', 'sam18', 'sam19']
self.l19_taxon_names = ['tax1', 'tax2', 'tax3', 'tax4', 'endbigtaxon',\
'tax6', 'tax7', 'tax8', 'tax9']
self.SampleMapping = ["OTU1\tsample1\t3", "OTU1\tsample3\t2", \
"OTU2\tsample1\t1", "OTU2\tsample2\t2"]
self.SampleMapping2 = ["OTU1\tsample1", "OTU1\tsample3", \
"OTU2\tsample1", "OTU2\tsample2"]
self.legacy_otu_table1 = legacy_otu_table1
self.otu_table1 = otu_table1
self.expected_lineages1 = expected_lineages1
self.taxa_summary1 = taxa_summary1
self.taxa_summary1_expected = taxa_summary1_expected
self.otu_table1_floats=otu_table1_floats
self.files_to_remove = []
self.denoiser_mapping1 = denoiser_mapping1.split('\n')
def tearDown(self):
remove_files(self.files_to_remove)
def test_parse_taxa_summary_table(self):
""" parse_taxa_summary_table functions as expected """
actual = parse_taxa_summary_table(self.taxa_summary1.split('\n'))
self.assertEqual(actual[0],self.taxa_summary1_expected[0])
self.assertEqual(actual[1],self.taxa_summary1_expected[1])
self.assertEqual(actual[2],self.taxa_summary1_expected[2])
self.assertEqual(actual,self.taxa_summary1_expected)
def test_parse_newick(self):
"""parse_newick correctly matches escaped tip names to otu ids
"""
# confirm that it works without escaped names
t1 = ('((((tax7:0.1,tax3:0.2):.98,tax8:.3, tax4:.3):.4,'
'((tax1:0.3, tax6:.09):0.43,tax2:0.4):0.5):.2,'
'(tax9:0.3, endbigtaxon:.08));')
expected1 = ['tax7','tax3','tax8','tax4','tax1',
'tax6','tax2','tax9','endbigtaxon']
self.assertEqual(set(parse_newick(t1).getTipNames()),set(expected1))
self.assertEqual(set([tip.Name for tip in parse_newick(t1).tips()]),
set(expected1))
# throw some screwed up names in
t2 = ('((((tax7:0.1,tax3:0.2):.98,tax8:.3, \'tax4\':.3):.4,'
"(('ta_______ x1':0.3, tax6:.09):0.43,tax2:0.4):0.5):.2,"
'(tax9:0.3, endbigtaxon:.08));')
expected2 = ['tax7','tax3','tax8','tax4','ta_______ x1',
'tax6','tax2','tax9','endbigtaxon']
self.assertEqual(set(parse_newick(t2).getTipNames()),set(expected2))
self.assertEqual(set([tip.Name for tip in parse_newick(t2).tips()]),
set(expected2))
def test_parse_mapping_file(self):
"""parse_mapping_file functions as expected"""
s1 = ['#sample\ta\tb', '#comment line to skip',\
'x \t y \t z ', ' ', '#more skip', 'i\tj\tk']
exp = ([['x','y','z'],['i','j','k']],\
['sample','a','b'],\
['comment line to skip','more skip'])
obs = parse_mapping_file(s1)
self.assertEqual(obs, exp)
# We don't currently support this, but we should soon...
# # check that first non-comment, non-blank line is used as
# # header
# s1 = ['sample\ta\tb', '#comment line to skip',\
# 'x \t y \t z ', ' ', '#more skip', 'i\tj\tk']
# exp = ([['x','y','z'],['i','j','k']],\
# ['sample','a','b'],\
# ['comment line to skip','more skip'])
# obs = parse_mapping_file(s1)
# self.assertEqual(obs, exp)
#check that we strip double quotes by default
s2 = ['#sample\ta\tb', '#comment line to skip',\
'"x "\t" y "\t z ', ' ', '"#more skip"', 'i\t"j"\tk']
obs = parse_mapping_file(s2)
self.assertEqual(obs, exp)
def test_mapping_file_to_dict(self):
"""parse_mapping_file functions as expected"""
s1 = ['#sample\ta\tb', '#comment line to skip',\
'x \t y \t z ', ' ', '#more skip', 'i\tj\tk']
exp = ([['x','y','z'],['i','j','k']],\
['sample','a','b'],\
['comment line to skip','more skip'])
mapres = parse_mapping_file(s1) # map_data, header, comments
mapdict = mapping_file_to_dict(*mapres[:2])
expdict = {'x':{'a':'y','b':'z'}, 'i':{'a':'j','b':'k'}}
self.assertEqual(mapdict, expdict)
def test_parse_mapping_file_to_dict(self):
"""parse_mapping_file functions as expected"""
s1 = ['#sample\ta\tb', '#comment line to skip',\
'x \t y \t z ', ' ', '#more skip', 'i\tj\tk']
exp = ([['x','y','z'],['i','j','k']],\
['sample','a','b'],\
['comment line to skip','more skip'])
mapdict, comments = parse_mapping_file_to_dict(s1)
expdict = {'x':{'a':'y','b':'z'}, 'i':{'a':'j','b':'k'}}
self.assertEqual(mapdict, expdict)
self.assertEqual(comments, ['comment line to skip','more skip'])
def test_parse_mapping_file_handles_filepath(self):
""" parse_mapping_file handles being passed a mapping filepath
"""
fp = get_tmp_filename(prefix='test_parse_mapping_file',
suffix='.txt')
self.files_to_remove.append(fp)
open(fp,'w').write('\n'.join(['#sample\ta\tb',
'#comment line to skip',
'x \t y \t z ', ' ',
'#more skip',
'i\tj\tk']))
obs = parse_mapping_file(fp)
exp = ([['x','y','z'],['i','j','k']],\
['sample','a','b'],\
['comment line to skip','more skip'])
self.assertEqual(obs, exp)
def test_parse_mapping_file_handles_file_handle(self):
""" parse_mapping_file handles being passed a mapping filepath
"""
fp = get_tmp_filename(prefix='test_parse_mapping_file',
suffix='.txt')
self.files_to_remove.append(fp)
open(fp,'w').write('\n'.join(['#sample\ta\tb',
'#comment line to skip',
'x \t y \t z ', ' ',
'#more skip',
'i\tj\tk']))
obs = parse_mapping_file(open(fp))
exp = ([['x','y','z'],['i','j','k']],\
['sample','a','b'],\
['comment line to skip','more skip'])
self.assertEqual(obs, exp)
def test_parse_mapping_file_handles_errors(self):
"""parse_mapping_file handles bad mapping files"""
# Empty file
self.assertRaises(QiimeParseError,
parse_mapping_file,
[])
# string
self.assertRaises(QiimeParseError,
parse_mapping_file,
'my_mapping_file.txt')
# invalid format (no header line with leading # sign)
self.assertRaises(QiimeParseError,
parse_mapping_file,
['sampleID\ta\tb',
'1\tf\t43',
'2\tt\t44'])
# invalid format (no non-header lines)
self.assertRaises(QiimeParseError,
parse_mapping_file,
['#sampleID\ta\tb'])
# invalid format (no header line)
self.assertRaises(QiimeParseError,
parse_mapping_file,
['1\tf\t43',
'2\tt\t44'])
def test_parse_prefs_file(self):
"""parse_prefs_file should correctly eval prefs string.
"""
#Test good input
ps1 = """{'bgcolor':'white','colors':
{'id':'blue','name':'green'},'list':[1,2,3]}"""
exp1 = {'bgcolor':'white','colors':{'id':'blue','name':'green'},\
'list':[1,2,3]}
self.assertEqual(parse_prefs_file(ps1),exp1)
#Test bad input
#list of valid input rather than multiline string should fail.
ps_bad_1 = ["{'bgcolor':'white',",
"'colors':{'id':'blue','name':'green'}",\
",'list':[1,2,3]}"]
self.assertRaises(QiimeParseError,parse_prefs_file,ps_bad_1)
#bad data. Can be evaluated but not a dict.
ps_bad_2 = "[1,2,3]"
self.assertRaises(QiimeParseError,parse_prefs_file,ps_bad_2)
def test_group_by_field(self):
"""group_by_field should group table by fields"""
t = [
['#sample', 'loc', 'age'],
['a','US','5'],
['b','US','10'],
['c','Mal','5'],
['d','Mal','10'],
['e','Ven','5'],
]
self.assertEqual(group_by_field(t, 'loc'), \
{'US':['a','b'], 'Mal':['c','d'], 'Ven':['e']})
self.assertEqual(group_by_field(t, 'age'), \
{'5':['a','c','e'], '10':['b','d']})
def test_group_by_fields(self):
"""group_by_fields should group table by fields"""
t = [
['#sample', 'loc', 'age', 'mal'],
['a','US','5','n'],
['b','US','10','n'],
['c','Mal','5','y'],
['d','Mal','10','n'],
['e','Mal','5','y'],
]
self.assertEqual(group_by_fields(t, ['age','loc']), \
{('5','US'):['a'], ('10','US'):['b'], ('5','Mal'):['c','e'],
('10','Mal'):['d']})
def test_parse_distmat(self):
"""parse_distmat should read distmat correctly"""
lines = """\ta\tb\tc
a\t0\t1\t2
b\t1\t0\t3.5
c\t1\t3.5\t0
""".splitlines()
exp = (['a','b','c'], array([[0,1,2],[1,0,3.5],[1,3.5,0]]))
obs = parse_distmat(lines)
self.assertEqual(obs, exp)
def test_parse_distmat_to_dict(self):
"""parse_distmat should return dict of distmat"""
lines = """\ta\tb\tc
a\t0\t1\t2
b\t1\t0\t3.5
c\t1\t3.5\t0
""".splitlines()
exp = {'a': {'a': 0.0, 'c': 2.0, 'b': 1.0},
'c': {'a': 1.0, 'c': 0.0, 'b': 3.5},
'b': {'a': 1.0, 'c': 3.5, 'b': 0.0}}
obs = parse_distmat_to_dict(lines)
self.assertEqual(obs, exp)
#should raise error because row and column headers don't match
wrong_dist_mat ="""\ta\ty\tx
a\t0\t1\t2
b\t1\t0\t3.5
c\t1\t3.5\t0
""".splitlines()
self.failUnlessRaises(AssertionError, parse_distmat_to_dict, wrong_dist_mat)
def test_parse_bootstrap_support(self):
"""parse_distmat should read distmat correctly"""
input_txt = """#\ta\tb\tc.
#more comments here
node2\t0
17node\t0.11922
"""
lines = input_txt.splitlines()
exp = {'17node':0.11922, 'node2':0.00}
obs = parse_bootstrap_support(lines)
self.assertFloatEqual(obs, exp)
def test_parse_rarefaction_data(self):
self.data = {}
self.data['headers'] = ['PD_whole_tree.txt', 'Antibiotics']
self.data['error'] = {'NA': [0.099969643842700004], \
'Y': [0.105669693476, 1.08546135424, 1.5626248357999999], \
'N': [0.101173002974]}
self.data['options'] = ['Y', 'NA', 'N']
self.data['xaxis'] = [10.0, 310.0, 610.0, 910.0, 1210.0, 1510.0, \
1810.0, 2110.0, 2410.0, 2710.0, 3010.0]
self.data['series'] = {'NA': [0.88581050485400004], \
'Y': [0.918845147059, 7.1758656176500004, 9.9186072941199992], \
'N': [0.92636763785999998]}
self.data['color'] = {'NA': '#00ff00', 'Y': '#ff0000', 'N': '#0000ff'}
self.rarefaction_series_data = ['# PD_whole_tree.txt',
'# Antibiotics',
'xaxis: 10.0\t310.0\t610.0\t910.0\t1210.0\t1510.0\t1810.0\t2110.0\
\t2410.0\t2710.0\t3010.0\t',
'xmax: 3310.0',
'>> Y',
'color #ff0000',
'series 0.918845147059\t7.17586561765\t9.91860729412\t',
'error 0.105669693476\t1.08546135424\t1.5626248358\t',
'>> NA',
'color #00ff00',
'series 0.885810504854\t',
'error 0.0999696438427\t',
'>> N',
'color #0000ff',
'series 0.92636763786\t',
'error 0.101173002974'
]
test = parse_rarefaction_data(self.rarefaction_series_data)
self.assertEqual(test, self.data)
def test_parse_rarefaction_record(self):
self.rarefactionline1 = 'rare10.txt\t10\t0\t1.99181\t0.42877\t2.13996'
test1 = parse_rarefaction_record(self.rarefactionline1)
self.rarefactiondata1 = ('rare10.txt', [10.0, 0.0, \
1.9918100000000001, 0.42876999999999998, 2.1399599999999999])
self.assertEqual(self.rarefactiondata1, test1)
self.rarefactionline2 = 'rare10.txt\t10\t0\t1.99181\t0.42877\tNA'
test2 = parse_rarefaction_record(self.rarefactionline2)
self.rarefactiondata2 = ('rare10.txt', [10.0, 0.0, 1.9918100000000001,\
0.42876999999999998, nan])
self.assertEqual(self.rarefactiondata2, test2)
def test_parse_rarefaction_fname(self):
""" parse_rarefaction_fname should return base, seqs/sam, iters, etc."""
fname = "alpha_rarefaction_900_3.txt"
base, seqs, iter, ext = parse_rarefaction_fname(fname)
self.assertEqual((base, seqs, iter, ext),
("alpha_rarefaction", 900, 3, ".txt"))
def test_parse_rarefaction(self):
self.rarefactionfile = ['\tsequences per sample\titeration\t123\t234\t345',
'rare10.txt\t10\t0\t1.99181\t0.42877\t2.13996',
'rare10.txt\t10\t1\t2.07163\t0.42877\t2.37055',
'rare310.txt\t310\t0\t8.83115\t0.42877\t11.00725',
'rare310.txt\t310\t1\t10.05242\t0.42877\t8.24474',
'rare610.txt\t610\t0\t12.03067\t0.42877\t11.58928',
'rare610.txt\t610\t1\t12.9862\t0.42877\t11.58642']
self.col_headers = ['', 'sequences per sample', 'iteration', '123', '234', '345']
self.comments = []
self.rarefaction_fns = ['rare10.txt', 'rare10.txt', 'rare310.txt', 'rare310.txt', 'rare610.txt', 'rare610.txt']
self.rarefaction_data = [[10.0, 0.0, 1.9918100000000001, 0.42876999999999998, 2.1399599999999999], [10.0, 1.0, 2.0716299999999999, 0.42876999999999998, 2.3705500000000002], [310.0, 0.0, 8.8311499999999992, 0.42876999999999998, 11.007250000000001], [310.0, 1.0, 10.05242, 0.42876999999999998, 8.2447400000000002], [610.0, 0.0, 12.030670000000001, 0.42876999999999998, 11.58928], [610.0, 1.0, 12.9862, 0.42876999999999998, 11.58642]]
test_col_headers, test_comments, test_rarefaction_fns, test_rarefaction_data = parse_rarefaction(self.rarefactionfile)
self.assertEqual(test_col_headers, self.col_headers)
self.assertEqual(test_comments, self.comments)
self.assertEqual(test_rarefaction_fns, self.rarefaction_fns)
self.assertEqual(test_rarefaction_data, self.rarefaction_data)
# def test_parse_rarefaction(self):
# """parse_rarefaction should handle multiple recs"""
# recs ="""#HEADER 97.0 NFkeyRightShift 1000
# #CHAO1 288.12903 241.27093 371.41733
# #ACE 294.74813 252.75930 361.30606 0.68654
# #SHANNON 3.71822 3.61146 3.82499
# #SIMPSON 0.08021
# #n rare rare_lci rare_hci
# 1 1.000000 1.000000 1.000000
# 51 26.878000 26.032290 27.723710
# #HEADER 97.0 DMkeySpace 1000
# #CHAO1 90.20000 53.81120 196.98011
# #ACE 122.66234 68.48901 264.46888 1.53571
# #SHANNON 1.35156 1.12549 1.57762
# #SIMPSON 0.56002
# #n rare rare_lci rare_hci
# 1 1.000000 1.000000 1.000000
# 51 10.707000 10.085986 11.328014
# 101 17.547000 17.046632 18.047368
# 151 23.410000 23.009805 23.810195
# #HEADER 97.0 RKkeyW 1000
# #CHAO1 251.57143 176.86438 401.83780
# #ACE 264.44294 192.23364 395.08515 0.89714
# #SHANNON 1.56521 1.44411 1.68630
# #SIMPSON 0.54772
# #n rare rare_lci rare_hci
# 1 1.000000 1.000000 1.000000
# 51 11.739000 11.060658 12.417342
# 101 19.054000 18.458137 19.649863
# 151 24.994000 24.447174 25.540826
# """.splitlines()
# obs = parse_rarefaction(recs)
# self.assertEqual(set(obs.keys()), \
# set(['NFkeyRightShift','DMkeySpace','RKkeyW']))
def test_parse_coords(self):
"""parse_coords should handle coords file"""
coords = """pc vector number\t1\t2\t3
A\t0.11\t0.09\t0.23
B\t0.03\t0.07\t-0.26
C\t0.12\t0.06\t-0.32
eigvals\t4.94\t1.79\t1.50
% variation explained\t14.3\t5.2\t4.3
""".splitlines()
obs = parse_coords(coords)
exp = (['A','B','C'],
array([[.11,.09,.23],[.03,.07,-.26],[.12,.06,-.32]]),
array([4.94,1.79,1.50]),
array([14.3,5.2,4.3]))
self.assertEqual(obs, exp)
def test_parse_otu_table_legacy(self):
"""parse_otu_table functions as expected with legacy OTU table
"""
data = self.legacy_otu_table1
data_f = (data.split('\n'))
obs = parse_otu_table(data_f)
exp = (['Fing','Key','NA'],
['0','1','2','3','4'],
array([[19111,44536,42],[1216,3500,6],[1803,1184,2],
[1722,4903,17], [589,2074,34]]),
self.expected_lineages1)
self.assertEqual(obs, exp)
def test_parse_otu_table(self):
"""parse_otu_table functions as expected with new-style OTU table
"""
data = self.otu_table1
data_f = (data.split('\n'))
obs = parse_otu_table(data_f)
exp = (['Fing','Key','NA'],
['0','1','2','3','4'],
array([[19111,44536,42],[1216,3500,6],[1803,1184,2],
[1722,4903,17], [589,2074,34]]),
self.expected_lineages1)
self.assertEqual(obs, exp)
def test_parse_otu_table_floats_in_table(self):
"""parse_otu_table functions using an OTU table containing floats
but cast as int....this will automatically cast into floats"""
data = self.otu_table1_floats
data_f = (data.split('\n'))
obs = parse_otu_table(data_f)
exp = (['Fing','Key','NA'],
['0','1','2','3','4'],
array([[19111.0,44536.0,42.0],[1216.0,3500.0,6.0],
[1803.0,1184.0,2.0],[1722.1,4903.2,17.0],
[589.6,2074.4,34.5]]),
self.expected_lineages1)
self.assertEqual(obs, exp)
def test_parse_otu_table_float_counts(self):
"""parse_otu_table should return correct result from small table"""
data = """#Full OTU Counts
#OTU ID Fing Key NA Consensus Lineage
0 19111 44536 42 Bacteria; Actinobacteria; Actinobacteridae; Propionibacterineae; Propionibacterium
1 1216 3500 6 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Lactobacillales; Lactobacillales; Streptococcaceae; Streptococcus
2 1803 1184 2 Bacteria; Actinobacteria; Actinobacteridae; Gordoniaceae; Corynebacteriaceae
3 1722 4903 17 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Staphylococcaceae
4 589 2074 34 Bacteria; Cyanobacteria; Chloroplasts; vectors"""
data_f = (data.split('\n'))
obs = parse_otu_table(data_f,count_map_f=float)
exp = (['Fing','Key','NA'],
['0','1','2','3','4'],
array([[19111.,44536.,42.],[1216.,3500.,6.],[1803.,1184.,2.],\
[1722.,4903.,17.], [589,2074.,34.]]),
[['Bacteria','Actinobacteria','Actinobacteridae','Propionibacterineae','Propionibacterium'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Lactobacillales','Lactobacillales','Streptococcaceae','Streptococcus'],
['Bacteria','Actinobacteria','Actinobacteridae','Gordoniaceae','Corynebacteriaceae'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Staphylococcaceae'],
['Bacteria','Cyanobacteria','Chloroplasts','vectors']])
self.assertEqual(obs, exp)
def test_parse_otu_table_file(self):
"""parse_otu_table should return correct result on fileio format object"""
data = """#Full OTU Counts
#OTU ID Fing Key NA Consensus Lineage
0 19111 44536 42 Bacteria; Actinobacteria; Actinobacteridae; Propionibacterineae; Propionibacterium
1 1216 3500 6 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Lactobacillales; Lactobacillales; Streptococcaceae; Streptococcus
2 1803 1184 2 Bacteria; Actinobacteria; Actinobacteridae; Gordoniaceae; Corynebacteriaceae
3 1722 4903 17 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Staphylococcaceae
4 589 2074 34 Bacteria; Cyanobacteria; Chloroplasts; vectors"""
data_f = StringIO(data)
obs = parse_otu_table(data_f)
exp = (['Fing','Key','NA'],
['0','1','2','3','4'],
array([[19111,44536,42],[1216,3500,6],[1803,1184,2],\
[1722,4903,17], [589,2074,34]]),
[['Bacteria','Actinobacteria','Actinobacteridae','Propionibacterineae','Propionibacterium'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Lactobacillales','Lactobacillales','Streptococcaceae','Streptococcus'],
['Bacteria','Actinobacteria','Actinobacteridae','Gordoniaceae','Corynebacteriaceae'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Staphylococcaceae'],
['Bacteria','Cyanobacteria','Chloroplasts','vectors']])
self.assertEqual(obs, exp)
def test_parse_otu_table_consensus_lineage(self):
"""parse_otu_table should accept 'consensusLineage'"""
data = """#Full OTU Counts
#OTU ID Fing Key NA consensusLineage
0 19111 44536 42 Bacteria; Actinobacteria; Actinobacteridae; Propionibacterineae; Propionibacterium
1 1216 3500 6 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Lactobacillales; Lactobacillales; Streptococcaceae; Streptococcus
2 1803 1184 2 Bacteria; Actinobacteria; Actinobacteridae; Gordoniaceae; Corynebacteriaceae
3 1722 4903 17 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Staphylococcaceae
4 589 2074 34 Bacteria; Cyanobacteria; Chloroplasts; vectors"""
data_f = StringIO(data)
obs = parse_otu_table(data_f)
exp = (['Fing','Key','NA'],
['0','1','2','3','4'],
array([[19111,44536,42],[1216,3500,6],[1803,1184,2],\
[1722,4903,17], [589,2074,34]]),
[['Bacteria','Actinobacteria','Actinobacteridae','Propionibacterineae','Propionibacterium'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Lactobacillales','Lactobacillales','Streptococcaceae','Streptococcus'],
['Bacteria','Actinobacteria','Actinobacteridae','Gordoniaceae','Corynebacteriaceae'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Staphylococcaceae'],
['Bacteria','Cyanobacteria','Chloroplasts','vectors']])
self.assertEqual(obs, exp)
def test_make_envs_dict(self):
""" make_envs_dict should have the same abundance for each taxon
as the matrix that made the dict"""
envs = make_envs_dict(self.l19_data, self.l19_sample_names,
self.l19_taxon_names)
for key in envs.keys():
col_idx = self.l19_taxon_names.index(key)
self.assertEqual(sum(envs[key].values()),
self.l19_data[:,col_idx].sum())
def test_fields_to_dict(self):
"""fields_to_dict should make first field key, rest val"""
test_data = \
"""0 R27DLI_4812 R27DLI_600 R27DLI_727 U1PLI_403 U1PLI_8969 U1PLI_9080 U1PLI_9526 W3Cecum_6642 W3Cecum_8992
1 U1PLI_7889
2 W3Cecum_4858
3 R27DLI_3243 R27DLI_4562 R27DLI_6828 R27DLI_9097 U1PLI_2780 U1PLI_67 U9PSI_10475 U9PSI_4341 W3Cecum_5191""".splitlines() #output from cd-hit
obs = fields_to_dict(test_data)
exp = {'0':['R27DLI_4812','R27DLI_600','R27DLI_727','U1PLI_403','U1PLI_8969','U1PLI_9080','U1PLI_9526','W3Cecum_6642','W3Cecum_8992'],
'1':['U1PLI_7889'],
'2':['W3Cecum_4858'],
'3':['R27DLI_3243','R27DLI_4562','R27DLI_6828','R27DLI_9097','U1PLI_2780','U1PLI_67','U9PSI_10475','U9PSI_4341','W3Cecum_5191']}
self.assertEqual(obs, exp)
def test_parse_qiime_parameters(self):
"""parse_qiime_parameters: functions with valid input """
lines = ["#Don't edit this file!",\
"pick_otus:similarity 0.94",\
"pick_otus:otu_picking_method\tcdhit",\
"align_seqs:verbose",\
"assign_taxonomy:use_rdp\ttRuE",\
"assign_taxonomy:something\tNone",\
"",\
"#some_script:fake_parameter\t99.0"]
actual = parse_qiime_parameters(lines)
expected = {'pick_otus':\
{'similarity':'0.94', 'otu_picking_method':'cdhit'},\
'assign_taxonomy':\
{'use_rdp':None}}
self.assertEqual(actual,expected)
# default dict functions as expected -- looking up non-existant key
# returns empty dict
self.assertEqual(actual['some_other_script'],{})
def test_parse_sample_mapping(self):
"""parse_sample_mapping works"""
lines = self.SampleMapping
OTU_sample_info, all_sample_names = parse_sample_mapping(lines)
self.assertEqual(OTU_sample_info, {'OTU2': {'sample1': '1', 'sample3': '0', 'sample2': '2'}, 'OTU1': {'sample1': '3', 'sample3': '2', 'sample2': '0'}})
self.assertEqual(all_sample_names, set(['sample1', 'sample3', 'sample2']))
#test that it works if no sample counts in there
lines = self.SampleMapping2
OTU_sample_info, all_sample_names = parse_sample_mapping(lines)
self.assertEqual(OTU_sample_info, {'OTU2': {'sample1': '1', 'sample3': '0', 'sample2': '1'}, 'OTU1': {'sample1': '1', 'sample3': '1', 'sample2': '0'}})
self.assertEqual(all_sample_names, set(['sample1', 'sample3', 'sample2']))
def test_sample_mapping_to_otu_table(self):
"""sample_mapping_to_otu_table works"""
lines = self.SampleMapping
result = sample_mapping_to_otu_table(lines)
self.assertEqual(result, ['#Full OTU Counts',\
'#OTU ID\tsample1\tsample2\tsample3', 'OTU2\t1\t2\t0', \
'OTU1\t3\t0\t2'])
def test_parse_taxonomy(self):
""" should parse taxonomy example, keeping otu id only"""
example_tax = \
"""412 PC.635_647 Root;Bacteria;Firmicutes;"Clostridia";Clostridiales 0.930
319 PC.355_281 Root;Bacteria;Bacteroidetes 0.970
353 PC.634_154 Root;Bacteria;Bacteroidetes 0.830
17 PC.607_302 Root;Bacteria;Bacteroidetes 0.960
13 PC.481_1214 Root;Bacteria;Firmicutes;"Clostridia";Clostridiales 0.870
338 PC.593_1314 Root;Bacteria 0.990 42556 Additional fields ignored"""
res = parse_taxonomy(example_tax.split('\n'))
self.assertEqual(res['412'],
"Root;Bacteria;Firmicutes;\"Clostridia\";Clostridiales")
self.assertEqual(res['338'],
"Root;Bacteria")
def test_parse_qiime_config_files(self):
""" parse_qiime_config_files functions as expected """
fake_file1 = ['key1\tval1','key2 val2']
fake_file2 = ['key2\tval3']
actual = parse_qiime_config_files([fake_file1,fake_file2])
expected = {'key1':'val1','key2':'val3'}
self.assertEqual(actual,expected)
# looking up a non-existant value returns None
self.assertEqual(actual['fake_key'],None)
# empty dict on empty input
self.assertEqual(parse_qiime_config_files([]),{})
def test_parse_metadata_state_descriptions(self):
"""parse_metadata_state_descriptions should return correct states from string."""
s = ''
self.assertEqual(parse_metadata_state_descriptions(s), {})
s = 'Study:Twin,Hand,Dog;BodySite:Palm,Stool'
self.assertEqual(parse_metadata_state_descriptions(s), {'Study':set(['Twin','Hand','Dog']),
'BodySite':set(['Palm','Stool'])})
def test_parse_illumina_line_barcode_in_header(self):
"""parse_illumina_line: handles barcode in header correctly """
illumina_line0 = illumina_read1[0]
illumina_line1 = illumina_read1[1]
actual = parse_illumina_line(
illumina_line0,barcode_length=6,rev_comp_barcode=True)
expected = {\
'Full description':'HWI-6X_9267:1:1:4:1699#ACCACCC/1',\
'Machine Name':'HWI-6X_9267',\
'Channel Number':1,\
'Tile Number':1,\
'X Position':4,\
'Y Position':1699,\
'Barcode':'GGTGGT',\
'Full Y Position Field':'1699#ACCACCC/1',\
'Sequence':\
'TACGGAGGGTGCGAGCGTTAATCGCCCCCCCCCCCCCCCCCCCCCCCCCCCC'+\
'CCCCCCCCCCCCCCCCCCCCCCCGAAAAAAAAAAAAAAAAAAAAAAA',\
'Quality Score':\
'abbbbbbbbbb`_`bbbbbb`bb^aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa'+\
'aaaaaaaaaaaaaDaabbBBBBBBBBBBBBBBBBBBB'}
self.assertEqual(actual,expected)
actual = parse_illumina_line(
illumina_line0,barcode_length=6,rev_comp_barcode=False)
expected['Barcode'] = 'ACCACC'
actual = parse_illumina_line(
illumina_line1,barcode_length=6,rev_comp_barcode=True)
expected = {\
'Full description':'HWI-6X_9267:1:1:4:390#ACCTCCC/1',\
'Machine Name':'HWI-6X_9267',\
'Channel Number':1,\
'Tile Number':1,\
'X Position':4,\
'Y Position':390,\
'Barcode':'GGAGGT',\
'Full Y Position Field':'390#ACCTCCC/1',\
'Sequence':\
'GACAGGAGGAGCAAGTGTTATTCAAATTATGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGG'+\
'GGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA',\
'Quality Score':\
'aaaaaaaaaa```aa\^_aa``aVaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa'+\
'aaaaaaaaaaaaaaaaaaaaaaaaaaaaaBaaaaa'}
self.assertEqual(actual,expected)
actual = parse_illumina_line(
illumina_line1,barcode_length=6,rev_comp_barcode=False)
expected['Barcode'] = 'ACCTCC'
def test_parse_illumina_line_barcode_in_sequence(self):
"""parse_illumina_line: handles barcode in sequence correctly """
illumina_line0 = illumina_read3[0]
actual = parse_illumina_line(
illumina_line0,barcode_length=12,
rev_comp_barcode=False,barcode_in_sequence=True)
expected = {\
'Full description':'HWI-EAS440_0386:1:23:19516:1031#0/1',
'Machine Name':'HWI-EAS440_0386',
'Channel Number':1,
'Tile Number':23,
'X Position':19516,
'Y Position':1031,
'Barcode':'ACAGCTAGCTTG',
'Full Y Position Field':'1031#0/1',
'Sequence':
'TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATT'
'GTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGT'
'ACAGTAGAGGCAGGCGGAATTCGTGGGG',
'Quality Score':
'fffcGddd\_``_gggggggggggfgggggegggggcgggggggggggeeffafdcdfgbdggdbe]fbf'
'dddddbdadadcddaf`abb`cVNRNUScaa``aOY]]]_[_BBBBBBBBBBB'
'BBBBBBBBBBBBBBBBBBBBBBBBBBBBB'}
self.assertEqual(actual,expected)
def test_parse_qual_score(self):
"""qual_score should return dict of {id: qual_scores}"""
scores = StringIO('>x\n5 10 5\n12\n>y\n30 40')
self.assertEqual(parse_qual_score(scores),
{'x':[5,10,5,12],'y':[30,40]})
#Check that a bad file, e.g. a fast raises Error
bad_scores = StringIO('>x\nabcbd\n12\n>y\GATC')
self.assertRaises(QiimeParseError, parse_qual_score, bad_scores)
def test_parse_qual_scores(self):
"""qual_scores should return dict of {id:qual_scores}"""
scores = StringIO('>x\n5 10 5\n12\n>y\n30 40')
scores2= StringIO('>a\n5 10 5\n12\n>b\n30 40')
self.assertEqual(parse_qual_scores([scores, scores2]),
{'x':[5,10,5,12],'y':[30,40],'a':[5,10,5,12],'b':[30,40]})
def test_MinimalQualParser(self):
"""MinimalQualParser should yield (id_, quals)"""
scores = ['>x','5 10 5','12',
'>y','30 40',
'>a','5 10 5','12',
'>b','30 40']
gen = MinimalQualParser(scores)
self.assertEqual(list(gen), [('x',[5,10,5,12]),
('y',[30,40]),
('a',[5,10,5,12]),
('b',[30,40])])
def test_parse_trflp(self):
""" should return a header and otu_table lists"""
data = \
""" Bin (10bp) Bin (20bp) Bin (30bp) Bin (40 bp)
Samp-le 1 1000 2000 3000 4000
Sample 2 2000 3000 4000
Sample 3 3000 4000
Sample 4 4000
Sample 5 25 """
samples, otus, data = parse_trflp(data.split('\n'))
samples_exp = ['Samp.le.1', 'Sample.2', 'Sample.3', 'Sample.4', 'Sample.5']
otus_exp = ['Bin__10bp_', 'Bin__20bp_', 'Bin__30bp_', 'Bin__40_bp_']
data_exp = array([[1000, 0, 0, 0, 25],\
[2000, 2000, 0, 0, 0],\
[3000, 3000, 3000, 0, 0],\
[4000, 4000, 4000, 4000, 0]])
self.assertEqual(samples, samples_exp)
self.assertEqual(otus, otus_exp)
self.assertEqual(data, data_exp)
def test_parse_trflp_headerless(self):
""" should return a header and otu_table lists"""
data = \
"""Samp-le 1 1000 2000 3000 4000
Sample 2 2000 3000 4000
Sample 3 3000 4000
Sample 4 4000
Sample_5__ 25 """
samples, otus, data = parse_trflp(data.split('\n'))
samples_exp = ['Samp.le.1', 'Sample.2', 'Sample.3', 'Sample.4', 'Sample.5..']
otus_exp = ['Bin__0', 'Bin__1', 'Bin__2', 'Bin__3']
data_exp = array([[1000, 0, 0, 0, 25],\
[2000, 2000, 0, 0, 0],\
[3000, 3000, 3000, 0, 0],\
[4000, 4000, 4000, 4000, 0]])
self.assertEqual(samples, samples_exp)
self.assertEqual(otus, otus_exp)
self.assertEqual(data, data_exp)
def test_parse_trflp_headerless_diff_row_len(self):
""" should return a header and otu_table lists"""
data = \
"""Samp-le 1 1000 2000
Sample 2 2000
Sample 3 3000
Sample 4 4000
Sample 5 25
"""
samples, otus, data = parse_trflp(data.split('\n'))
samples_exp = ['Samp.le.1', 'Sample.2', 'Sample.3', 'Sample.4', 'Sample.5']
otus_exp = ['Bin__0', 'Bin__1', 'Bin__2', 'Bin__3']
data_exp = array([[1000, 0, 0, 0, 25],\
[2000, 2000, 0, 0, 0],\
[0, 0, 3000, 0, 0],\
[0, 0, 0, 4000, 0]])
self.assertEqual(samples, samples_exp)
self.assertEqual(otus, otus_exp)
self.assertEqual(data, data_exp)
def test_parse_denoiser_mapping(self):
""" parse_denoiser_mapping creates {} from denoiser mapping file
"""
actual = parse_denoiser_mapping(self.denoiser_mapping1)
expected = {'Read1':['Read1','Read4','Read5 some comment'],
'Read2':['Read2'],
'Read3':['Read3','Read6']}
self.assertEqual(actual,expected)
illumina_read1 = """HWI-6X_9267:1:1:4:1699#ACCACCC/1:TACGGAGGGTGCGAGCGTTAATCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGAAAAAAAAAAAAAAAAAAAAAAA:abbbbbbbbbb`_`bbbbbb`bb^aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaDaabbBBBBBBBBBBBBBBBBBBB
HWI-6X_9267:1:1:4:390#ACCTCCC/1:GACAGGAGGAGCAAGTGTTATTCAAATTATGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA:aaaaaaaaaa```aa\^_aa``aVaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaBaaaaa""".split('\n')
illumina_read2 = """HWI-6X_9267:1:1:4:1699#ACCACCC/2:TTTTAAAAAAAAGGGGGGGGGGGCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTTTTAAAAAAAAACCCCCCCGGGGGGGGTTTTTTTAATTATTC:aaaaaaaaaaaaaaaaaaaaaaaaaaaaabbbbbbbbbbbbbbbbbbbbbbbbbcccccccccccccccccBcccccccccccccccc```````BBBB
HWI-6X_9267:1:1:4:390#ACCTCCC/2:ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACG:aaaaaaaaaaaaaaaaaaaaaaaaaabbbbbbbbbbbbbbbbbbbbbbbbbbbbbaaaaaaaaaaaaaaaaaaaaabbbbbbbbbbbbbbbbbbbbbbb""".split('\n')
illumina_read3 = """HWI-EAS440_0386:1:23:19516:1031#0/1:ACAGCTAGCTTGTACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGCGGAATTCGTGGGG:gggggggeggcffffcGddd\_``_gggggggggggfgggggegggggcgggggggggggeeffafdcdfgbdggdbe]fbfdddddbdadadcddaf`abb`cVNRNUScaa``aOY]]]_[_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
HWI-EAS440_0386:1:23:19660:1034#0/1:CATATCGCAGTTTACGNAAGGTCCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTAGGCGGGTAAGCAAGTTAGAAGTGAAATCCTATAGCTCAACTATAGTAAGCTTTTAAAACTGCTCATCTTGAGGTATGGAAGGGAAAGTGGAATTCCTAGTTA:fhhghhhhfhghhhhcHdcddccddhhhhhhfhhhhghhhdghhhhhhhhhhfhhhdhghgghhhhhhbfdfdbagdgdgfffafa]dad_acdabZcaabad[a__^_`cbddefb_cd^]_L\]U_]^aaZ___]bBBBBBBBBBBBBBBBBBBBBBBBBBB""".split('\n')
legacy_otu_table1 = """# some comment goes here
#OTU ID Fing Key NA Consensus Lineage
0 19111 44536 42 Bacteria; Actinobacteria; Actinobacteridae; Propionibacterineae; Propionibacterium
1 1216 3500 6 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Lactobacillales; Lactobacillales; Streptococcaceae; Streptococcus
2 1803 1184 2 Bacteria; Actinobacteria; Actinobacteridae; Gordoniaceae; Corynebacteriaceae
3 1722 4903 17 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Staphylococcaceae
4 589 2074 34 Bacteria; Cyanobacteria; Chloroplasts; vectors
"""
otu_table1 = """# Some comment
OTU ID Fing Key NA Consensus Lineage
0 19111 44536 42 Bacteria; Actinobacteria; Actinobacteridae; Propionibacterineae; Propionibacterium
# some other comment
1 1216 3500 6 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Lactobacillales; Lactobacillales; Streptococcaceae; Streptococcus
2 1803 1184 2 Bacteria; Actinobacteria; Actinobacteridae; Gordoniaceae; Corynebacteriaceae
# comments
# everywhere!
3 1722 4903 17 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Staphylococcaceae
4 589 2074 34 Bacteria; Cyanobacteria; Chloroplasts; vectors
"""
otu_table1_floats = """# Some comment
OTU ID Fing Key NA Consensus Lineage
0 19111.0 44536.0 42.0 Bacteria; Actinobacteria; Actinobacteridae; Propionibacterineae; Propionibacterium
# some other comment
1 1216.0 3500.0 6.0 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Lactobacillales; Lactobacillales; Streptococcaceae; Streptococcus
2 1803.0 1184.0 2.0 Bacteria; Actinobacteria; Actinobacteridae; Gordoniaceae; Corynebacteriaceae
# comments
# everywhere!
3 1722.1 4903.2 17 Bacteria; Firmicutes; Alicyclobacillaceae; Bacilli; Staphylococcaceae
4 589.6 2074.4 34.5 Bacteria; Cyanobacteria; Chloroplasts; vectors
"""
taxa_summary1 = """#Full OTU Counts
Taxon Even1 Even2 Even3
Bacteria;Actinobacteria;Actinobacteria(class);Actinobacteridae 0.0880247251673 0.0721968465746 0.081371761759
Bacteria;Bacteroidetes/Chlorobigroup;Bacteroidetes;Bacteroidia 0.192137761955 0.191095101593 0.188504131885
Bacteria;Firmicutes;Bacilli;Lactobacillales 0.0264895739603 0.0259942669171 0.0318460745596
# some comment
Bacteria;Firmicutes;Clostridia;Clostridiales 0.491800007824 0.526186212556 0.49911159984
Bacteria;Firmicutes;Erysipelotrichi;Erysipelotrichales 0.0311411916592 0.0184083913576 0.0282325481054
Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales 0.166137214246 0.163087129528 0.168923372865
No blast hit;Other 0.00426952518811 0.00303205147361 0.0020105109874"""
taxa_summary1_expected = (['Even1','Even2','Even3'],
['Bacteria;Actinobacteria;Actinobacteria(class);Actinobacteridae',
'Bacteria;Bacteroidetes/Chlorobigroup;Bacteroidetes;Bacteroidia',
'Bacteria;Firmicutes;Bacilli;Lactobacillales',
'Bacteria;Firmicutes;Clostridia;Clostridiales',
'Bacteria;Firmicutes;Erysipelotrichi;Erysipelotrichales',
'Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales',
'No blast hit;Other'],
array([[0.0880247251673, 0.0721968465746, 0.081371761759],
[0.192137761955, 0.191095101593, 0.188504131885],
[0.0264895739603, 0.0259942669171, 0.0318460745596],
[0.491800007824, 0.526186212556, 0.49911159984],
[0.0311411916592, 0.0184083913576, 0.0282325481054],
[0.166137214246, 0.163087129528, 0.168923372865],
[0.00426952518811, 0.00303205147361, 0.0020105109874]]))
expected_lineages1 = [['Bacteria','Actinobacteria','Actinobacteridae','Propionibacterineae','Propionibacterium'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Lactobacillales','Lactobacillales','Streptococcaceae','Streptococcus'],
['Bacteria','Actinobacteria','Actinobacteridae','Gordoniaceae','Corynebacteriaceae'],
['Bacteria','Firmicutes','Alicyclobacillaceae','Bacilli','Staphylococcaceae'],
['Bacteria','Cyanobacteria','Chloroplasts','vectors']]
denoiser_mapping1 = """Read1:\tRead4\tRead5 some comment
Read2:
Read3:\tRead6"""
if __name__ =='__main__':
main()
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