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#!/usr/bin/perl
#-------------------------------------------------------------------------------
#
# Create an MA-plot
# Data is feed as two column of numbers
#
# Note: Any line that does not match a numeric regular expression, is filtered out).
#
# Pablo Cingolani
#-------------------------------------------------------------------------------
#-------------------------------------------------------------------------------
# Main
#-------------------------------------------------------------------------------
# Parse command line option (file base name)
$base = 'maPlot';
if( $ARGV[0] ne '' ) { $base = $ARGV[0]; }
$pngFile = "$base.png";
$txtFile = "$base.txt";
# Read STDIN and create an R table
open TXT, "> $txtFile" or die "Cannot open output file '$txtFile'\n";
print TXT "x\ty\n";
for( $ln = 0 ; $l = <STDIN> ; ) {
chomp $l;
($x, $y) = split /\t/, $l;
# Does the string contain exactly one number? (can be float)
if(( $x =~ /^[-+]?[0-9]*\.?[0-9]+([eE][-+]?[0-9]+)?$/ ) && ( $y =~ /^[-+]?[0-9]*\.?[0-9]+([eE][-+]?[0-9]+)?$/ )) { print TXT "$x\t$y\n"; }
}
close TXT;
#---
# Create an R program, save histogram plot as PNG image
#---
open R, "| R --vanilla --slave " or die "Cannot open R program\n";
print R <<EOF;
maPlot <- function( logr, logg, title ) {
m <- logr - logg;
a <- 1/2 * (logr + logg);
smoothScatter(a, m, main=title, ylab='M', xlab='A');
lines( lowess(a, m), col='orange' );
}
png('$pngFile', width = 1024, height = 1024);
data <- read.csv("$txtFile", sep='\\t', header = TRUE);
x <- data\$x
y <- data\$y
maPlot(x, y, "MA plot");
dev.off();
quit( save='no' )
EOF
close R;
#---
# Show figure
#---
$os = `uname`;
$show = "eog";
if( $os =~ "Darwin" ) { $show = "open"; }
`$show $pngFile`;
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