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#include <tk.h>
#include <string.h>
#include <stdlib.h>
#include <float.h>
#include "io-reg.h"
#include "template_display.h"
#include "IO1.h"
#include "canvas_box.h"
#include "gap_canvas_box.h"
#include "newgap_cmds.h"
#include "gap_globals.h"
#include "misc.h"
#include "reading_coverage.h"
#include "readpair_coverage.h"
#include "tclXkeylist.h"
#include "text_output.h"
#include "tcl_utils.h"
#include "consen.h"
#include "qual.h"
#include "consistency_display.h"
#include "text_output.h"
#include "ruler_display.h"
#include "template.h"
/*
* Maintains readpair_coverage with the contig registration scheme.
* The histogram is drawn as part of a consistency display
*/
/*
*---------------------------------------------------------------------------
* Prototypes
*---------------------------------------------------------------------------
*/
static void readpair_coverage_callback(GapIO *io, int contig, void *fdata,
reg_data *jdata);
/*
*---------------------------------------------------------------------------
* Internal functions
*---------------------------------------------------------------------------
*/
/*
*/
void plot_readpair_coverage(Tcl_Interp *interp,
int *histogram,
int seq_len,
char *c_win,
GapIO *io,
int offset,
int width,
char *colour,
int min,
int max)
{
int i, start;
char cmd[1024];
start = 1;
for (i = 2; i <= seq_len; i++) {
#ifdef DEBUG
printf("h[%d]=%d\n", i, histogram[i]);
#endif
if (histogram[i] != histogram[i-1]) {
/* horizonal line */
sprintf(cmd, "%s create line %d %d %d %d -fill %s -width %d",
c_win, start+offset-1, max - histogram[start] + min,
i-1+offset-1, max - histogram[i-1] + min, colour, width);
#ifdef DEBUG
printf("%s\n", cmd);
#endif
Tcl_Eval(interp, cmd);
/* vertical line */
sprintf(cmd, "%s create line %d %d %d %d -fill %s -width %d",
c_win, i-1+offset-1, max - histogram[i-1] + min,
i+offset-1, max - histogram[i] + min, colour, width);
#ifdef DEBUG
printf("%s\n", cmd);
#endif
Tcl_Eval(interp, cmd);
start = i;
}
}
/* last horizonal line */
sprintf(cmd, "%s create line %d %d %d %d -fill %s -width %d",
c_win, start+offset-1, max - histogram[start] + min,
i-1+offset-1, max - histogram[i-1] + min, colour, width);
#ifdef DEBUG
printf("%s\n", cmd);
#endif
Tcl_Eval(interp, cmd);
}
int calc_readpair_coverage(GapIO *io,
int contig,
int start,
int end,
int *histogram, /* out */
int *min, /* out */
int *max) /* out */
{
int i, j;
int x1, x2, tx1, tx2;
template_c *t;
template_c **tarr;
int status;
int ntemplates = Ntemplates(io);
item_t *item;
gel_cont_t *gc;
int tmp;
tx1 = INT_MAX;
tx2 = INT_MIN;
if (ntemplates == 0)
return -1;
/* Initialise templates */
if (NULL == (tarr = init_template_checks(io, 1, &contig, 1)))
return -1;
check_all_templates(io, tarr);
/* for each template */
for (i = 1; i <= ntemplates; i++){
t = tarr[i];
/* if the template is in the contig */
if (t) {
for (item = head(t->gel_cont); item; item=item->next) {
gc = (gel_cont_t *)(item->data);
if (gc->contig != contig)
continue;
tmp = t->consistency;
get_template_positions(io, t, gc->contig);
t->consistency |= tmp;
status = getStatus(t);
if (status == FORW_REV_READINGS) {
x1 = MIN(MIN(t->start, t->end), t->min);
x2 = MAX(MAX(t->start, t->end), t->max);
#ifdef DEBUG
printf("FORW_REV_READINGS %d x1 %d x2 %d\n", i, x1, x2);
#endif
for (j = x1; j <= x2; j++) {
if (j >= start && j <= end) {
histogram[j-start+1]++;
if (histogram[j-start] > *max)
*max = histogram[j-start];
if (histogram[j-start] < *min)
*min = histogram[j-start];
}
}
}
break;
}
}
}
if (tarr)
uninit_template_checks(io, tarr);
/* always set minimum to 0 */
*min = 0;
return 0;
}
/*
* plot the vertical ruler
*/
void plot_readpair_coverage_ruler(Tcl_Interp *interp,
obj_reading_coverage *rcov,
CanvasPtr *canvas,
WorldPtr *world)
{
display_ruler_v(interp, canvas, rcov->ruler, rcov->t_min, rcov->t_max);
scaleSingleCanvas(interp, world, canvas, rcov->ruler->window, 'y', "all");
}
/*
* readpair_coverage histogram
*/
void display_readpair_coverage(GapIO *io, obj_reading_coverage *rcov) {
char cmd[1024];
int i;
obj_consistency_disp *c;
int length;
int win_num;
c = result_data(io, rcov->cons_id, 0);
sprintf(cmd, "%s delete all", rcov->c_win);
Tcl_Eval(c->interp, cmd);
win_num = get_consistency_win_num(c, rcov->id);
for (i = 0; i < c->num_contigs; i++) {
if (c->num_contigs > 1) {
length = ABS(io_clength(io, c->contigs[i]));
} else {
length = c->end - c->start + 1;
}
plot_readpair_coverage(c->interp, rcov->histogram1[i], length,
rcov->c_win, io,
c->start+c->contig_offset[c->contigs[i]].offset,
rcov->linewidth, rcov->colour1, rcov->t_min,
rcov->t_max);
}
plot_readpair_coverage_ruler(c->interp, rcov, c->win_list[win_num]->canvas,
c->win_list[win_num]->world);
scaleCanvas(c->interp, &c->win_list[win_num], 1, "all",
c->win_list[win_num]->world->visible,
c->win_list[win_num]->canvas);
scrollRegion(c->interp, &c->win_list[win_num], 1,
c->win_list[win_num]->world->total,
c->win_list[win_num]->canvas);
consistency_update_cursors(io, c, 0);
}
/*
* Removes the readpair_coverage (and unplots etc).
*/
static void readpair_coverage_shutdown(GapIO *io, obj_reading_coverage *rcov) {
char cmd[1024];
int i;
obj_consistency_disp *c;
int win_num;
if (NULL != (c = result_data(io, rcov->cons_id, 0))) {
win_num = get_consistency_win_num(c, rcov->id);
if (win_num != -1) {
delete_consistency_window(c, win_num);
}
}
for (i = 0; i < c->num_contigs; i++) {
contig_deregister(io, c->contigs[i], readpair_coverage_callback,
(void *)rcov);
}
sprintf(cmd, "DeleteReadPairCoverage %d %s %s %d\n", *handle_io(io),
rcov->frame, rcov->c_win, rcov->cons_id);
if (TCL_ERROR == Tcl_Eval(c->interp, cmd))
printf("readpair_coverage_shutdown: %s\n", Tcl_GetStringResult(c->interp));
if (rcov->histogram1) {
for (i = 0; i < c->num_contigs; i++) {
xfree(rcov->histogram1[i]);
}
xfree(rcov->histogram1);
}
if (rcov->min)
xfree(rcov->min);
if (rcov->max)
xfree(rcov->max);
free_ruler_struct(rcov->ruler);
xfree(rcov);
if (c->num_wins == 0)
consistency_shutdown(io, c);
}
/*
* Updates an obj_reading_coverage structure.
* This includes recalculating the length, reallocing the memory used,
* and recalculating the consensus sequence.
*
* Returns 0 for success, -1 for failure.
*/
static int update_obj_readpair_coverage(GapIO *io,
obj_reading_coverage *rcov,
int contig) {
int i, j, ret, newl;
obj_consistency_disp *c;
int start, end;
int win_num;
#ifdef DEBUG
printf("UPDATE_OBJ_READPAIR_COVERAGE\n");
#endif
c = result_data(io, rcov->cons_id, 0);
/* find contig index */
for (i = 0; i < c->num_contigs; i++) {
if (c->contigs[i] == contig) {
break;
}
}
/*
* if single contig, start & end are a range otherwise set start & end
* to length of contig
*/
if (c->num_contigs == 1) {
start = c->start;
end = c->end;
} else {
start = 1;
end = ABS(io_clength(io, c->contigs[i]));
}
newl = end - start + 1;
if (rcov->histogram1[i]) {
if (NULL == (rcov->histogram1[i] =
(int *)xrealloc(rcov->histogram1[i], (newl+1)*sizeof(int))))
return -1;
}
/* re-initialise */
rcov->max[i] = INT_MIN;
rcov->min[i] = INT_MAX;
rcov->t_max = INT_MIN;
rcov->t_min = INT_MAX;
for (j = 0; j <= newl; j++) {
rcov->histogram1[i][j] = 0;
}
ret = calc_readpair_coverage(io, c->contigs[i], start, end,
rcov->histogram1[i], &rcov->min[i],
&rcov->max[i]);
/* find total min and max over all contigs */
for (i = 0; i < c->num_contigs; i++) {
if (rcov->t_max < rcov->max[i])
rcov->t_max = rcov->max[i];
}
rcov->t_min = 0;
/* need to reset y coords */
win_num = get_consistency_win_num(c, rcov->id);
c->win_list[win_num]->world->total->y1 = rcov->t_min;
c->win_list[win_num]->world->total->y2 = rcov->t_max;
c->win_list[win_num]->world->visible->y1 = rcov->t_min;
c->win_list[win_num]->world->visible->y2 = rcov->t_max;
return ret;
}
#if 0
static void readpair_coverage_list(GapIO *io,
obj_consistency_disp *c,
obj_reading_coverage *rcov,
int contig)
{
int i, j;
int length;
for (i = 0; i < c->num_contigs; i++) {
if (c->num_contigs > 1) {
length = ABS(io_clength(io, c->contigs[i]));
} else {
length = c->end - c->start + 1;
}
vmessage("Contig %s (#%d)\n",
get_contig_name(io, contig), io_clnbr(io, contig));
for (j = 0; j < length; j++) {
vmessage("%d %d\n", c->start+j, rcov->histogram1[i][j]);
}
vmessage("\n");
}
}
#endif
/* scroll the readpair_coverage ruler */
static void readpair_coverageScrollY(Tcl_Interp *interp,
obj_reading_coverage *rcov,
CanvasPtr *canvas,
d_box *visible,
char *scroll_args)
{
char cmd[1024];
double wx;
sprintf(cmd, "eval %s yview %s", rcov->ruler->window, scroll_args);
Tcl_Eval(interp, cmd);
/* find new top edge of canvas in canvasy coords */
Tcl_VarEval(interp, rcov->ruler->window, " canvasy 0", NULL);
canvas->y = atoi(Tcl_GetStringResult(interp));
/* find new top and bottom edges of canvas in world coords */
CanvasToWorld(canvas, 0, canvas->y, &wx, &visible->y1);
CanvasToWorld(canvas, 0, canvas->y + canvas->height, &wx, &visible->y2);
SetCanvasCoords(interp, visible->x1, visible->y1,
visible->x2, visible->y2, canvas);
}
static void readpair_coverage_join(GapIO *io,
obj_consistency_disp *c,
obj_reading_coverage *rcov,
int index)
{
int length;
length = c->num_contigs - index - 1;
memmove(&(rcov->histogram1)[index], &(rcov->histogram1)[index+1],
length * sizeof(int*));
memmove(&(rcov->min)[index], &(rcov->min)[index+1],
length * sizeof(int));
memmove(&(rcov->max)[index], &(rcov->max)[index+1],
length * sizeof(int));
}
/*
* The callback from the contig registration scheme.
*/
static void readpair_coverage_callback(GapIO *io, int contig, void *fdata,
reg_data *jdata) {
obj_reading_coverage *rcov = (obj_reading_coverage *)fdata;
obj_consistency_disp *c;
int win_num;
c = result_data(io, rcov->cons_id, 0);
switch(jdata->job) {
case REG_QUERY_NAME:
{
sprintf(jdata->name.line, "Readpair coverage histogram");
return;
}
case REG_COMPLEMENT:
case REG_LENGTH:
{
#ifdef DEBUG
printf("readpair_coverage COMPLEMENT LENGTH contig %d\n", contig);
#endif
if (update_obj_readpair_coverage(io, rcov, contig) == 0) {
display_readpair_coverage(io, rcov);
return;
}
/* else
* flow through to REG_QUIT
*/
}
case REG_QUIT:
case REG_DELETE:
{
#ifdef DEBUG
printf("readpair_coverage QUIT DELETE \n");
#endif
readpair_coverage_shutdown(io, rcov);
return;
}
case REG_GET_OPS:
{
jdata->get_ops.ops =
#if 0
"List\0"
"SEPARATOR\0"
#endif
"Remove\0";
return;
}
case REG_INVOKE_OP:
{
switch (jdata->invoke_op.op) {
#if 0
case 0: /* List Quality */
start_message();
readpair_coverage_list(io, c, rcov, contig);
end_message();
break;
#endif
case 0: /* Remove */
readpair_coverage_shutdown(io, rcov);
break;
}
return;
}
case REG_PARAMS:
{
puts("REG_PARAMS");
return;
}
case REG_GENERIC:
switch (jdata->generic.task) {
case TASK_CONS_ID:
jdata->generic.data = (void *)rcov->cons_id;
case TASK_DISPLAY_TICKS:
{
/* int *ticks = (int *)jdata->generic.data; */
char cmd[100];
int win_num;
#ifdef DEBUG
printf("Readpair_Coverage display_ticks\n");
#endif
/* get readpair_coverage window */
win_num = get_consistency_win_num(c, rcov->id);
sprintf(cmd, "%s delete all", rcov->ruler->window);
Tcl_Eval(c->interp, cmd);
plot_readpair_coverage_ruler(c->interp, rcov,
c->win_list[win_num]->canvas,
c->win_list[win_num]->world);
break;
}
case TASK_CANVAS_SCROLLY:
{
char *scroll = (char *)jdata->generic.data;
win_num = get_consistency_win_num(c, rcov->id);
/* scroll the ruler */
readpair_coverageScrollY(c->interp, rcov,
c->win_list[win_num]->canvas,
c->win_list[win_num]->world->visible,
scroll);
/* scroll the readpair_coverage plot */
consistencyScrollY(c->interp, rcov->c_win,
c->win_list[win_num]->scroll,
c->win_list[win_num]->world->visible,
c->win_list[win_num]->canvas, scroll);
break;
}
case TASK_CANVAS_REDRAW:
#ifdef DEBUG
printf("readpair_coverage TASK_CANVAS_REDRAW\n");
#endif
display_readpair_coverage(io, rcov);
break;
case TASK_CANVAS_RESIZE:
{
char cmd[1024];
int win_num;
/* resize readpair_coverage window */
#ifdef DEBUG
printf("Readpair_Coverage TASK_CANVAS_RESIZE\n");
#endif
win_num = get_consistency_win_num(c, rcov->id);
sprintf(cmd, "%s delete all", rcov->ruler->window);
Tcl_Eval(c->interp, cmd);
plot_readpair_coverage_ruler(c->interp, rcov,
c->win_list[win_num]->canvas,
c->win_list[win_num]->world);
}
break;
case TASK_CANVAS_WORLD:
{
task_world_t *tw = (task_world_t *)jdata->generic.data;
int cx = tw->canvasx;
double wx, wy;
#ifdef DEBUG
printf("READPAIR_COVERAGE TASK_CANVAS_WORLD\n");
#endif
win_num = get_consistency_win_num(c, rcov->id);
CanvasToWorld(c->win_list[win_num]->canvas, cx, 0, &wx, &wy);
tw->basex = wx - c->contig_offset[tw->cnum].offset;
break;
}
case TASK_CONS_WORLD:
{
d_box *cw = (d_box *)jdata->generic.data;
win_num = get_consistency_win_num(c, rcov->id);
cw->x1 = c->win_list[win_num]->world->total->x1;
cw->y1 = c->win_list[win_num]->world->total->y1;
cw->x2 = c->win_list[win_num]->world->total->x2;
cw->y2 = c->win_list[win_num]->world->total->y2;
break;
}
case TASK_CONS_JOIN:
{
#ifdef DEBUG
printf("readpair_coverage JOIN_TO contig %d join %d\n",
contig, jdata->join.contig);
#endif
readpair_coverage_join(io, c, rcov, (int)jdata->generic.data);
break;
}
case TASK_CONS_CURSOR_DELETE:
{
#ifdef DEBUG
printf("READPAIR_COVERAGE cursor_delete\n");
#endif
Tcl_VarEval(c->interp, rcov->c_win, " delete cursor_y", NULL);
break;
}
case TASK_CANVAS_CURSOR_Y:
{
char *label;
int *cy = (int *)jdata->generic.data;
double wx, wy;
int win_num;
win_num = get_consistency_win_num(c, rcov->id);
CanvasToWorld(c->win_list[win_num]->canvas, 0, *cy, &wx, &wy);
wy = rcov->t_max - wy + rcov->t_min;
label = get_default_string(c->interp, gap_defs,
"CONSISTENCY_DISPLAY.CURSORY");
canvasCursorY(c->interp, c->win_list[win_num]->canvas,
c->frame, label,
c->xhair.colour, c->xhair.width, *cy, wy,
c->win_list, c->num_wins);
break;
}
}
}
}
/*
* Registers and initialises a quality buffer for a particular contig.
*/
int readpair_coverage_reg(GapIO *io,
Tcl_Interp *interp,
char *frame,
char *rcov_win,
int cons_id,
ruler_s *ruler)
{
obj_reading_coverage *rcov;
obj_consistency_disp *c;
int id;
int i, j;
int start, end, length;
char *val;
c = result_data(io, cons_id, 0);
/* first check that there are enough available windows */
if (c->num_wins >= MAX_NUM_WINS)
return -1;
if (NULL == (rcov = (obj_reading_coverage*)xmalloc(sizeof(obj_reading_coverage))))
return -1;
if (NULL == (rcov->histogram1 = (int **)xmalloc(c->num_contigs*sizeof(int*))))
return -1;
if (NULL == (rcov->min = (int *)xmalloc(c->num_contigs*sizeof(int))))
return -1;
if (NULL == (rcov->max = (int *)xmalloc(c->num_contigs*sizeof(int))))
return -1;
id = register_id();
rcov->id = id;
rcov->cons_id = cons_id;
strcpy(rcov->c_win, rcov_win);
strcpy(rcov->frame, frame);
rcov->linewidth = get_default_int(interp, gap_defs,
"READPAIR_COVERAGE.LINEWIDTH");
val = get_default_string(interp, gap_defs, "READPAIR_COVERAGE.COLOUR");
strcpy(rcov->colour1, val);
rcov->t_max = INT_MIN;
rcov->t_min = INT_MAX;
rcov->ruler = ruler;
for (i = 0; i < c->num_contigs; i++) {
if (c->num_contigs > 1) {
length = ABS(io_clength(io, c->contigs[i]));
start = 1;
end = length;
} else {
length = c->end - c->start + 1;
start = c->start;
end = c->end;
}
if (NULL==(rcov->histogram1[i]=(int *)xmalloc((length+1)*sizeof(int)))) {
return -1;
}
/* initialise histogram and min and max values */
for (j = 0; j <= length; j++) {
rcov->histogram1[i][j] = 0;
}
rcov->max[i] = INT_MIN;
rcov->min[i] = INT_MAX;
calc_readpair_coverage(io, c->contigs[i], start, end,
rcov->histogram1[i],
&rcov->min[i], &rcov->max[i]);
/* find total min and max over all contigs */
if (rcov->t_max < rcov->max[i]) {
rcov->t_max = rcov->max[i];
}
rcov->t_min = 0;
}
if (rcov->t_max == INT_MIN) {
vmessage("No read pairs within contigs have been found\n");
readpair_coverage_shutdown(io, rcov);
return -2;
}
add_consistency_window(io, c, rcov_win, 'b', id, c->orig_total->x1,
rcov->t_min, c->orig_total->x2, rcov->t_max);
display_readpair_coverage(io, rcov);
for (i = 0; i < c->num_contigs; i++) {
contig_register(io, c->contigs[i], readpair_coverage_callback,
(void *)rcov, id,
REG_REQUIRED | REG_DATA_CHANGE | REG_OPS |
REG_CURSOR_NOTIFY | REG_GENERIC,
REG_TYPE_READPAIR_COVERAGE);
}
#ifdef DEBUG
printf("READPAIR_COVERAGE ID %d\n", id);
#endif
return id;
}
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