1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289
|
#include <tcl.h>
#include "edUtils.h"
#include "tman_interface.h"
#include <io_lib/Read.h>
#include "misc.h"
#include "gap_globals.h"
#include "tclXkeylist.h"
#include "tcl_utils.h"
#include "tkTraceIO.h"
#include "mutlib.h"
/* FIXME: move to io_lib as it's used in convert_trace too */
static void rescale_heights(Read *r, int min_marker) {
double marker = 0;
int i, j, max, mtv = 0;
TRACE *tx[4];
tx[0] = r->traceA;
tx[1] = r->traceC;
tx[2] = r->traceG;
tx[3] = r->traceT;
for (i = 0; i < r->NPoints; i++) {
for (max = j = 0; j < 4; j++)
if (max < tx[j][i])
max = tx[j][i];
if (!marker) {
marker = max;
} else {
if (max >= marker) {
/* attack */
marker += (max - marker) / 20.0;
} else {
/* decay */
marker -= (marker - max) / 10.0;
}
}
if (marker < min_marker)
marker = min_marker;
for (j = 0; j < 4; j++) {
double new = tx[j][i] * 2000.0/marker;
tx[j][i] = new > 32767 ? 32767 : new;
if (mtv < tx[j][i])
mtv = tx[j][i];
}
}
r->maxTraceVal = mtv;
}
static Read *diff_readings(EdStruct *xx,
Read *r1, int seq1, int off1,
Read *r2, int seq2, int off2,
int *startp, int *start1p) {
int start1, end1, start2, end2, start, end;
Read *r;
tracediff_t td;
Read *norm1 = NULL, *norm2 = NULL;
/* One consensus trace works well, but not two. */
if (!seq1 && !seq2)
return NULL;
/* Sequences must be in the same orientation */
if (DB_Comp(xx, seq1) != DB_Comp(xx, seq2))
return NULL;
if (r1 == NULL || r2 == NULL)
return NULL;
/*
* Compute start and end of the overlap point in each reading. This is
* done using the non cutoff data only. We take 1 off the end position
* to ensure that we always have a next base to find the position of.
*/
/* This sets start and end to be positions in the consensus */
if (xx->diff_trace_size) {
start = positionInContig(xx, xx->cursorSeq, xx->cursorPos)
- xx->diff_trace_size;
start1 = MAX(start, DB_RelPos(xx, seq1) - DB_Start(xx, seq1)-1);
start2 = MAX(start, DB_RelPos(xx, seq2) - DB_Start(xx, seq2)-1);
end = positionInContig(xx, xx->cursorSeq, xx->cursorPos)
+ xx->diff_trace_size;
end1 = MIN(end, DB_RelPos(xx, seq1) - DB_Start(xx, seq1)-1
+ DB_Length2(xx, seq1) - 1);
end2 = MIN(end, DB_RelPos(xx, seq2) - DB_Start(xx, seq2)-1
+ DB_Length2(xx, seq2) - 1);
} else {
if (xx->reveal_cutoffs) {
start1 = DB_RelPos(xx, seq1) - DB_Start(xx, seq1)-1;
start2 = DB_RelPos(xx, seq2) - DB_Start(xx, seq2)-1;
end1 = DB_RelPos(xx, seq1) - DB_Start(xx, seq1)-1
+ DB_Length2(xx, seq1) - 1;
end2 = DB_RelPos(xx, seq2) - DB_Start(xx, seq2)-1
+ DB_Length2(xx, seq2) - 1;
} else {
start1 = DB_RelPos(xx, seq1);
start2 = DB_RelPos(xx, seq2);
end1 = DB_RelPos(xx, seq1) + DB_Length(xx, seq1) - 1;
end2 = DB_RelPos(xx, seq2) + DB_Length(xx, seq2) - 1;
}
}
start = MAX(start1, start2);
end = MIN(end1, end2);
start = MAX(start, 1);
end = MAX(end, 1);
start = MIN(start, DB_Length(xx, 0));
end = MIN(end, DB_Length(xx, 0));
if (end <= start) {
/*
* No overlap, but return a blank Read structure so that the
* trace display plots all traces correctly and lines up in 2x3 or 4x3
* column-x-row modes.
*/
return read_allocate(0, 0);
}
/* Now we convert these to positions in the full-length editor sequences */
start1 = start - (DB_RelPos(xx, seq1)-1) + DB_Start(xx, seq1);
start2 = start - (DB_RelPos(xx, seq2)-1) + DB_Start(xx, seq2);
end1 = end - (DB_RelPos(xx, seq1)-1) + DB_Start(xx, seq1);
end2 = end - (DB_RelPos(xx, seq2)-1) + DB_Start(xx, seq2);
/* Change from first/last used base to last/first clipped base */
start1--;
start2--;
end1++;
end2++;
/*
* And now we convert these to positions in the trace (orig orientation)
* When comparing against a consensus trace we just use the entire lot
* as this is how it's been generated in the first place (we hope).
*/
if (seq1) {
start1 = origpos(xx, seq1, start1);
end1 = origpos(xx, seq1, end1);
} else {
end1 -= start1;
start1 = 0;
}
if (seq2) {
start2 = origpos(xx, seq2, start2);
end2 = origpos(xx, seq2, end2);
} else {
end2 -= start2;
start2 = 0;
}
/*
* If complemented, change the start and end positions so that they map
* on to a trace counting with base 1 at the left, as the tracediff
* library does not have access to the trace display widget details (which
* contains the real numbering order).
*/
if (start1 > end1) {
start1 = r1->NBases - start1 + 1;
end1 = r1->NBases - end1 + 1;
}
if (start2 > end2) {
start2 = r2->NBases - start2 + 1;
end2 = r2->NBases - end2 + 1;
}
*startp = start;
/* Initialise Mark's trace diff code */
TraceDiffInit(&td);
if (xx->compare_trace_yscale) {
norm1 = read_dup(r1, NULL);
norm2 = read_dup(r2, NULL);
rescale_heights(norm1, 200);
rescale_heights(norm2, 200);
TraceDiffSetReference(&td, norm2, MUTLIB_STRAND_FORWARD, start2, end2);
TraceDiffSetInput(&td, norm1, MUTLIB_STRAND_FORWARD, start1, end1);
} else {
TraceDiffSetReference(&td, r1, MUTLIB_STRAND_FORWARD, start2, end2);
TraceDiffSetInput(&td, r2, MUTLIB_STRAND_FORWARD, start1, end1);
}
/* Do the difference without analysis of the results */
TraceDiffExecute(&td, TRACEDIFF_ALGORITHM_DEFAULT_DIFFERENCE_ONLY);
if (TraceDiffGetResultCode(&td)) {
verror(ERR_WARN, "diff_readings", "%s", TraceDiffGetResultString(&td));
return NULL;
}
/* Get a copy of the result and then destroy the TraceDiff instance */
r = TraceDiffGetDifference(&td,start1p, NULL);
if (!seq1) {
*start1p += start2-1;
}
if (r) {
r = read_dup(r, NULL);
/* set baseline and maxTraceVal */
/* diff_reset_zero(r); */
}
TraceDiffDestroy(&td);
if (norm1)
read_deallocate(norm1);
if (norm2)
read_deallocate(norm2);
return r;
}
/* ------------------------------------------------------------------------- */
/*
* Given two already loaded traces (ed1 and ed2), produce a new trace display
* containing the traces differences over the region that the traces overlap.
*/
DisplayContext *diff_edc_traces(EdStruct *xx, tman_dc *ed1, tman_dc *ed2) {
Tcl_CmdInfo info;
Read *r1, *r2, *r;
char *pname;
Tcl_Interp *interp = EDINTERP(xx->ed);
tman_dc *ed;
DisplayContext *dc;
static int diff_counter = 0;
int exists;
char buf[1024], name[1024];
int start = 0, start1;
/* Get the two read structures */
Tcl_GetCommandInfo(interp, ed1->dc->path, &info);
r1 = ((DNATrace *)info.clientData)->read;
Tcl_GetCommandInfo(interp, ed2->dc->path, &info);
r2 = ((DNATrace *)info.clientData)->read;
/* Produce a diff read structure */
r = diff_readings(xx, r1, ed1->seq, ed1->pos, r2, ed2->seq, ed2->pos,
&start, &start1);
if (r == NULL) {
bell();
return NULL;
}
/* Create a trace display */
pname = get_default_string(interp, gap_defs, "TRACE_DISPLAY.WIN");
if (ed1->seq)
sprintf(name, " {diffs: #%d #%d}",
DB_Number(xx, ed1->seq), DB_Number(xx, ed2->seq));
else
sprintf(name, " {diffs: =%d #%d}",
-DB_Number(xx, ed1->seq), DB_Number(xx, ed2->seq));
Tcl_VarEval(interp, "trace_create ",
Tk_PathName(EDTKWIN(xx->ed)), pname, " ",
Tk_PathName(EDTKWIN(xx->ed)),
name, NULL);
pname = Tcl_GetStringResult(interp);
/* Fill out the tman_dc and DisplayContext structures */
sprintf(buf, "Diffs %d", diff_counter++);
dc = getTDisplay(xx, buf, 0, 0, &exists);
strcpy(dc->path, pname);
ed = find_free_edc();
ed->dc = dc;
ed->pos = start-1;
ed->xx = xx;
ed->type = TRACE_TYPE_DIFF;
ed->derivative_seq = ed1->seq ? ed1->seq : ed2->seq;
ed->derivative_offset = start1;
/* Add the Read to the trace widget */
Tcl_GetCommandInfo(interp, Tcl_GetStringResult(interp), &info);
trace_memory_load((DNATrace *)info.clientData, r);
dc->tracePtr = (DNATrace *)info.clientData;
/* Adjust position */
{
int num, end;
int pos = positionInContig(xx, xx->cursorSeq, xx->cursorPos);
num = tman_get_trace_position(xx, ed, pos, &end);
repositionSeq(xx, dc, num);
}
return dc;
}
|