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//
// sharedrabundvectors.cpp
// Mothur
//
// Created by Sarah Westcott on 5/15/17.
// Copyright © 2017 Schloss Lab. All rights reserved.
//
#include "sharedrabundvectors.hpp"
/***********************************************************************/
//reads a shared file
SharedRAbundVectors::SharedRAbundVectors(ifstream& f, vector<string>& userGroups, string& nextLabel, string& labelTag) : DataVector(){
try {
int num, count;
count = 0;
string holdLabel, groupN;
int numUserGroups = userGroups.size();
for (int i = 0; i < lookup.size(); i++) { if (lookup[i] != nullptr) { delete lookup[i]; lookup[i] = nullptr; } } lookup.clear();
//are we at the beginning of the file??
if (nextLabel == "") {
f >> label;
//is this a shared file that has headers
if (label == "label") {
//gets "group"
f >> label; gobble(f);
//gets "numOtus"
f >> label; gobble(f);
//eat rest of line
label = util.getline(f); gobble(f);
//parse labels to save
istringstream iStringStream(label);
while(!iStringStream.eof()){
if (m->getControl_pressed()) { break; }
string temp;
iStringStream >> temp; gobble(iStringStream);
currentLabels.push_back(temp);
}
if (currentLabels.size() != 0) {
string binLabelTag = currentLabels[0];
labelTag = "";
for (int i = 0; i < binLabelTag.length(); i++) { if (isalpha(binLabelTag[i])){ labelTag += binLabelTag[i]; } }
}
f >> label >> groupN >> num;
if (currentLabels.size() != 0) {
if (currentLabels.size() != num) {
m->mothurOut("[ERROR]: your shared file contains " + toString(currentLabels.size()) + " OTU labels, but your numOtus column indicates " + toString(num) + ". Cannot continue, please correct. This can be caused by editing your file incorrectly outside of mothur.\n"); m->setControl_pressed(true);
}
}
}else {
//read in first row since you know there is at least 1 group.
f >> groupN >> num;
//make binlabels because we don't have any
string snumBins = toString(num);
if (labelTag == "") { labelTag = "Otu"; }
for (int i = 0; i < num; i++) {
//if there is a bin label use it otherwise make one
string binLabel = labelTag;
string sbinNumber = toString(i+1);
if (sbinNumber.length() < snumBins.length()) {
int diff = snumBins.length() - sbinNumber.length();
for (int h = 0; h < diff; h++) { binLabel += "0"; }
}
binLabel += sbinNumber;
currentLabels.push_back(binLabel);
}
}
}else {
label = nextLabel;
//read in first row since you know there is at least 1 group.
f >> groupN >> num;
}
//reset labels, currentLabels may have gotten changed as otus were eliminated because of group choices or sampling
//m->setCurrentSharedBinLabels(m->getSharedBinLabelsInFile());
holdLabel = label;
numBins = num;
bool readData = false;
bool remove = false;
if (numUserGroups == 0) { //user has not specified groups, so we will use all of them
userGroups.push_back(groupN);
readData = true;
}else{
if (util.inUsersGroups(groupN, userGroups)) { readData = true; }
else { remove = true; }
}
if (readData) {
//add new vector to lookup
SharedRAbundVector* temp = new SharedRAbundVector(f, label, groupN, numBins);
push_back(temp);
} else { util.getline(f); }
gobble(f);
if (!(f.eof())) {
f >> nextLabel;
}
//read the rest of the groups info in
while ((nextLabel == holdLabel) && (f.eof() != true)) {
f >> groupN >> num;
bool readData = false;
if (numUserGroups == 0) { //user has not specified groups, so we will use all of them
userGroups.push_back(groupN);
readData = true;
}else{
if (util.inUsersGroups(groupN, userGroups)) { readData = true; }
else { remove = true; }// skipline because you are a group we dont care about
}
if (readData) {
//add new vector to lookup
SharedRAbundVector* temp = new SharedRAbundVector(f, label, groupN, numBins);
push_back(temp);
} else { util.getline(f); }
gobble(f);
if (f.eof() != true) { f >> nextLabel; }
}
if (remove) { eliminateZeroOTUS(); }
otuTag = labelTag;
//error in names of user inputted Groups
if (lookup.size() < userGroups.size()) { m->mothurOut("[ERROR]: requesting groups not present in files, aborting.\n"); m->setControl_pressed(true); }
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "SharedRAbundVectors");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::print(ostream& output, bool& printOTUHeaders){
try {
printHeaders(output, printOTUHeaders);
sort(lookup.begin(), lookup.end(), compareRAbunds);
for (int i = 0; i < lookup.size(); i++) {
if (m->getControl_pressed()) { break; }
lookup[i]->print(output);
}
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "print");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::printTidy(ostream& output, bool& printOTUHeaders, bool keepZero){
try {
vector<string> otuLabels = getOTUNames();
if (printOTUHeaders) { output << "label\tsample\tOTU\tabundance\n"; printOTUHeaders = false; }
sort(lookup.begin(), lookup.end(), compareRAbunds);
for (int i = 0; i < lookup.size(); i++) {
if (m->getControl_pressed()) { break; }
vector<int> data = lookup[i]->get();
string thisGroup = lookup[i]->getGroup();
//if keepzero=false, zeroed otus are not outputted
for (int j = 0; j < data.size(); j++) {
if ((data[j] != 0) || (keepZero)) {
output << label << '\t' << thisGroup << '\t' << otuLabels[j] << '\t' << data[j] << endl;
}
}
}
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "printTidy");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::setOTUNames(vector<string> names){
try {
currentLabels.clear();
currentLabels = names;
getOTUNames();
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "setOTUNames");
exit(1);
}
}
/***********************************************************************/
string SharedRAbundVectors::getOTUName(int bin){
try {
if (currentLabels.size() > bin) { }
else { getOTUNames(); }
return currentLabels[bin];
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "getOTUName");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::setOTUName(int bin, string otuName){
try {
if (currentLabels.size() > bin) { currentLabels[bin] = otuName; }
else {
getOTUNames(); //fills currentLabels if needed
if (currentLabels.size() > bin) { currentLabels[bin] = otuName; }
else {
m->setControl_pressed(true);
m->mothurOut("[ERROR]: " + toString(bin) + " bin does not exist\n");
}
}
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "setOTUName");
exit(1);
}
}
/***********************************************************************/
vector<string> SharedRAbundVectors::getOTUNames(){
try {
util.getOTUNames(currentLabels, numBins, otuTag);
return currentLabels;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "getOTUNames");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::printHeaders(ostream& output, bool& printSharedHeaders){
try {
if (printSharedHeaders) {
getOTUNames();
output << "label\tGroup\tnum" + otuTag + "s";
for (int i = 0; i < numBins; i++) { output << '\t' << currentLabels[i]; } output << endl;
printSharedHeaders = false;
}
}
catch(exception& e) {
m->errorOut(e, "SharedVector", "printHeaders");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::push_back(SharedRAbundVector* thisLookup){
try {
if (numBins == 0) { numBins = thisLookup->getNumBins(); }
else if (numBins != thisLookup->getNumBins()) {
m->mothurOut("[ERROR]: Number of bins does not match. Expected " + toString(numBins) + " found " + toString(thisLookup->getNumBins()) + ".\n");
m->setControl_pressed(true); return 0;
}
lookup.push_back(thisLookup);
sort(lookup.begin(), lookup.end(), compareRAbunds);
if (label == "") { label = thisLookup->getLabel(); }
groupNames.clear();
for (int i = 0; i < lookup.size(); i ++) { groupNames[lookup[i]->getGroup()] = i; }
return ((int)lookup.size());
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "push_back");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::push_back(vector<int> abunds, string binLabel){
try {
if (abunds.size() != lookup.size()) { m->mothurOut("[ERROR]: you have provided " + toString(abunds.size()) + " abundances, but mothur was expecting " + toString(lookup.size()) + ", please correct.\n"); m->setControl_pressed(true); return 0; }
for (int i = 0; i < lookup.size(); i ++) { lookup[i]->push_back(abunds[i]); }
if (binLabel == "") { //create one
int otuNum = 1; bool notDone = true;
//find label prefix
string prefix = "Otu";
if (currentLabels.size() != 0) {
if (currentLabels[currentLabels.size()-1][0] == 'P') { prefix = "PhyloType"; }
string tempLabel = currentLabels[currentLabels.size()-1];
string simpleLastLabel = util.getSimpleLabel(tempLabel);
util.mothurConvert(simpleLastLabel, otuNum); otuNum++;
}
string potentialLabel = toString(otuNum);
while (notDone) {
if (m->getControl_pressed()) { notDone = false; break; }
potentialLabel = toString(otuNum);
vector<string>::iterator it = find(currentLabels.begin(), currentLabels.end(), potentialLabel);
if (it == currentLabels.end()) {
potentialLabel = prefix + toString(otuNum);
it = find(currentLabels.begin(), currentLabels.end(), potentialLabel);
if (it == currentLabels.end()) {
notDone = false; break;
}
}
otuNum++;
}
binLabel = potentialLabel;
}
currentLabels.push_back(binLabel);
numBins++;
return lookup.size();
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "push_back");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::getOTUTotal(int bin){
try {
int totalOTUAbund = 0;
for (int i = 0; i < lookup.size(); i++) { totalOTUAbund += lookup[i]->get(bin); }
return totalOTUAbund;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "getOTUTotal");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::getOTUTotal(string otuLabel){
try {
//find bin number
int binNumber = -1;
getOTUNames();
for (int i = 0; i < currentLabels.size(); i++) {
if (util.getSimpleLabel(currentLabels[i]) == util.getSimpleLabel(otuLabel)) {
binNumber = i; break;
}
}
if (binNumber == -1) { return 0; }
return getOTUTotal(binNumber);
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "getOTUTotal");
exit(1);
}
}
/***********************************************************************/
vector<int> SharedRAbundVectors::getOTU(int bin){
try {
vector<int> abunds;
for (int i = 0; i < lookup.size(); i++) { abunds.push_back(lookup[i]->get(bin)); }
return abunds;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "push_back");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::setLabels(string l){
try {
label = l;
for (int i = 0; i < lookup.size(); i++) { lookup[i]->setLabel(l); }
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "setLabels");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::get(int bin, string group){
try {
int abund = 0;
map<string, int>::iterator it = groupNames.find(group);
if (it == groupNames.end()) { m->mothurOut("[ERROR]: can not find group " + group + ".\n"); m->setControl_pressed(true); }
else { abund = lookup[it->second]->get(bin); }
return abund;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "get");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::getNumSeqs(string group){
try {
int numSeqs = 0;
map<string, int>::iterator it = groupNames.find(group);
if (it == groupNames.end()) { m->mothurOut("[ERROR]: can not find group " + group + ".\n"); m->setControl_pressed(true); }
else { numSeqs = lookup[it->second]->getNumSeqs(); }
return numSeqs;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "getNumSeqs");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::set(int bin, int binSize, string group){
try {
map<string, int>::iterator it = groupNames.find(group);
if (it == groupNames.end()) { m->mothurOut("[ERROR]: can not find group " + group + ".\n"); m->setControl_pressed(true); }
else { lookup[it->second]->set(bin, binSize); }
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "set");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::removeOTU(int bin){
try {
int totalOTUAbund = 0;
for (int i = 0; i < lookup.size(); i ++) {
totalOTUAbund += lookup[i]->remove(bin);
}
currentLabels.erase(currentLabels.begin()+bin);
numBins--;
return totalOTUAbund;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "removeOTU");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::removeOTUs(vector<int> bins, bool sorted){
try {
if (bins.size() == 0) { return 0; }
if (!sorted) { sort(bins.begin(), bins.end()); }
int totalOTUAbund = 0;
for (int i = 0; i < lookup.size(); i ++) {
totalOTUAbund += lookup[i]->remove(bins);
}
vector<string> newLabels; int binIndex = 0;
for (int i = 0; i < currentLabels.size(); i++) {
if (m->getControl_pressed()) { break; }
if (i != bins[binIndex]) {
newLabels.push_back(currentLabels[i]);
}else if (i == bins[binIndex]) {
binIndex++;
if (binIndex > bins.size()) { //removed all bins
newLabels.insert(newLabels.end(), currentLabels.begin()+i, currentLabels.end()); //add rest of good bins
break;
}
}
}
currentLabels = newLabels;
numBins = currentLabels.size();
return totalOTUAbund;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "removeOTUs");
exit(1);
}
}
/***********************************************************************/
vector<string> SharedRAbundVectors::getNamesGroups(){
try {
vector<string> names; names.clear();
for (int i = 0; i < lookup.size(); i ++) { names.push_back(lookup[i]->getGroup()); }
return names;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "getNamesGroups");
exit(1);
}
}
/***********************************************************************/
SharedOrderVector SharedRAbundVectors::getSharedOrderVector(){
try {
SharedOrderVector order;
for (int i = 0; i < lookup.size(); i++) {
for (int j = 0; j < lookup[i]->getNumBins(); j++) {
int abund = lookup[i]->get(j);
if (abund != 0) {
for (int k = 0; k < abund; k++) { order.push_back(j, lookup[i]->getGroup()); }
}
}
}
return order;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getSharedOrderVector");
exit(1);
}
}
/***********************************************************************/
void SharedRAbundVectors::removeGroups(vector<string> g){
try {
bool remove = false;
for (vector<SharedRAbundVector*>::iterator it = lookup.begin(); it != lookup.end();) {
//if this sharedrabund is not from a group the user wants then delete it.
if (util.inUsersGroups((*it)->getGroup(), g)) {
remove = true;
delete (*it); (*it) = nullptr;
it = lookup.erase(it);
}else { ++it; }
}
if (remove) { eliminateZeroOTUS(); }
groupNames.clear();
for (int i = 0; i < lookup.size(); i ++) { groupNames[lookup[i]->getGroup()] = i; }
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "removeGroups");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::removeGroups(int minSize, bool silent){
try {
vector<string> Groups;
bool remove = false;
for (vector<SharedRAbundVector*>::iterator it = lookup.begin(); it != lookup.end();) {
if ((*it)->getNumSeqs() < minSize) {
if (!silent) { m->mothurOut((*it)->getGroup() + " contains " + toString((*it)->getNumSeqs()) + ". Eliminating.\n"); }
delete (*it); (*it) = nullptr;
it = lookup.erase(it);
remove = true;
}else {
Groups.push_back((*it)->getGroup());
++it;
}
}
if (remove) { eliminateZeroOTUS(); }
groupNames.clear();
for (int i = 0; i < lookup.size(); i ++) { groupNames[lookup[i]->getGroup()] = i; }
return lookup.size();
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "removeGroups");
exit(1);
}
}
/***********************************************************************/
int SharedRAbundVectors::getNumSeqsSmallestGroup(){
try {
int smallest = MOTHURMAX;
for (int i = 0; i < lookup.size(); i++) {
if (m->getDebug()) { m->mothurOut("[DEBUG]: " + lookup[i]->getGroup() + " numSeqs = " + toString(lookup[i]->getNumSeqs()) + "\n"); }
if (lookup[i]->getNumSeqs() < smallest) { smallest = lookup[i]->getNumSeqs(); }
}
return smallest;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getNumSeqsSmallestGroup");
exit(1);
}
}
/***********************************************************************/
vector<SharedRAbundVector*> SharedRAbundVectors::getSharedRAbundVectors(){
try {
vector<SharedRAbundVector*> newLookup;
for (int i = 0; i < lookup.size(); i++) {
if (m->getControl_pressed()) { return newLookup; }
SharedRAbundVector* temp = new SharedRAbundVector(*lookup[i]);
newLookup.push_back(temp);
}
return newLookup;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getSharedRAbundVectors");
exit(1);
}
}
/***********************************************************************/
vector<SharedRAbundFloatVector*> SharedRAbundVectors::getSharedRAbundFloatVectors(){
try {
eliminateZeroOTUS();
vector<SharedRAbundFloatVector*> newLookup;
for (int i = 0; i < lookup.size(); i++) {
if (m->getControl_pressed()) { return newLookup; }
vector<float> abunds;
vector<int> data = lookup[i]->get();
string group = lookup[i]->getGroup();
for (int j = 0; j < data.size(); j++) { abunds.push_back((float)data[j]); }
SharedRAbundFloatVector* temp = new SharedRAbundFloatVector(abunds);
temp->setLabel(label);
temp->setGroup(group);
newLookup.push_back(temp);
}
return newLookup;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getSharedRAbundVectors");
exit(1);
}
}
/***********************************************************************/
RAbundVector SharedRAbundVectors::getRAbundVector(){
try {
RAbundVector rav; rav.setLabel(label);
for (int i = 0; i < numBins; i++) {
int abund = getOTUTotal(i);
rav.push_back(abund);
}
return rav;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getSharedRAbundVectors");
exit(1);
}
}
/***********************************************************************/
RAbundVector SharedRAbundVectors::getRAbundVector(string group){
try {
RAbundVector rav;
rav.setLabel(label);
for (vector<SharedRAbundVector*>::iterator it = lookup.begin(); it != lookup.end();) {
//if this sharedrabund is not from a group the user wants then delete it.
if ((*it)->getGroup() == group) {
for (int i = 0; i < (*it)->getNumBins(); i++) { rav.push_back((*it)->get(i)); }
return rav;
}else { ++it; }
}
return rav;
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getRAbundVector");
exit(1);
}
}
/***********************************************************************/
SAbundVector SharedRAbundVectors::getSAbundVector(){
try {
RAbundVector rav = getRAbundVector();
return rav.getSAbundVector();
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getSAbundVector");
exit(1);
}
}
/***********************************************************************/
SAbundVector SharedRAbundVectors::getSAbundVector(string group){
try {
RAbundVector rav = getRAbundVector(group);
return rav.getSAbundVector();
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVector", "getSAbundVector");
exit(1);
}
}
/**********************************************************************************************************************/
void SharedRAbundVectors::eliminateZeroOTUS() {
try {
if (currentLabels.size() != numBins) { currentLabels = getOTUNames(); }
if (lookup.size() > 1) {
vector<int> otusToRemove;
for (int i = 0; i < lookup[0]->getNumBins(); i++) {
if (m->getControl_pressed()) { break; }
int total = getOTUTotal(i);
//if they are not all zero add this bin
if (total == 0) { otusToRemove.push_back(i); } //sorted order
}
removeOTUs(otusToRemove, true); //sorted
}
}
catch(exception& e) {
m->errorOut(e, "SharedRAbundVectors", "eliminateZeroOTUS");
exit(1);
}
}
/***********************************************************************/
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